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Human umbilical cord mesenchymal stem cell exosome type external cream and preparation method thereof

A technology of stem cells and human umbilical cord, applied in the field of stem cells, can solve problems such as inability to cure the root cause, and achieve the effect of improving skin quality, moisturizing skin tissue, and scavenging free radicals

Inactive Publication Date: 2018-03-30
南京九圣生物医学科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, external cosmetics on the market only achieve the purpose of beauty by covering up defects or acting on the surface of the skin, but they only treat the symptoms, not the root cause

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Prepare DMEM / F12 culture solution, which contains 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0043] Soak the umbilical cord in 75% ethanol for 4 minutes, soak it in PBS and wash it 3 times, remove the blood vessels and cut them into 1cm 2 Small pieces, immersed in 0.1% type II collagenase, digested at 37°C for 50 minutes, added 0.25% trypsin, digested at 37°C for 10 minutes, added serum to stop trypsin digestion, filtered the aforementioned cell tissue fluid, centrifuged, and discarded Supernatant, remove the lower layer of cells;

[0044] Add DMEM / F12 culture medium to the lower layer of cells for cell counting, reaching 10 6 / ml after primary culture, according to 5×10 6 cells / ml were inoculated into T75 cell culture flasks, and the volume of DMEM / F12 culture medium in each cell culture flask was 20 ml, placed in CO 2 Culture in a 37°C incubator with a concentration of 5% by volume. Change the medium after 3 days to remove non-adherent cells, and then...

Embodiment 2

[0056] Prepare DMEM / F12 culture solution, which contains 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0057] Soak the umbilical cord in 75% ethanol for 4 minutes, soak it in PBS and wash it 3 times, remove the blood vessels and cut them into 2cm 2 Small pieces, submerged in 0.1% type II collagenase, digested at 37°C for 70 minutes, added 0.25% trypsin, digested at 37°C for 20 minutes, added serum to stop trypsin digestion, filtered the aforementioned cell tissue fluid, centrifuged, and discarded Supernatant, remove the lower layer of cells;

[0058] Add DMEM / F12 culture medium to the lower layer of cells for cell counting, reaching 10 7 / ml after primary culture, press 1×10 6 cells / ml were inoculated into T2 cell culture flasks, and the volume of DMEM / F12 culture medium in each cell culture flask was 5 ml, placed in CO 2 Culture in a 37°C incubator with a concentration of 5% by volume, change the medium after 3 days to remove non-adherent cells, then change the ...

Embodiment 3

[0070] Prepare DMEM / F12 culture solution, which contains 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0071] Soak the umbilical cord in 75% ethanol for 4 minutes, soak it in PBS and wash it 3 times, remove the blood vessels and cut into 1.5cm 2 Small pieces, immersed in 0.1% type II collagenase, digested at 37°C for 60 minutes, added 0.25% trypsin, digested at 37°C for 15 minutes, added serum to stop trypsin digestion, filtered the aforementioned cell tissue fluid, centrifuged, and discarded Supernatant, remove the lower layer of cells;

[0072] Add DMEM / F12 culture medium to the lower layer of cells for cell counting, reaching 10 6 / ml after primary culture, press 3×10 6 cells / ml was inoculated into T75 cell culture flasks, and the volume of DMEM / F12 culture medium in each cell culture flask was 15 ml, placed in CO 2 Culture in a 37°C incubator with a concentration of 5% by volume. Change the medium after 3 days to remove non-adherent cells, and then change the...

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Abstract

The invention provides human umbilical cord mesenchymal stem cell exosome type external cream and a preparation method thereof, and aims at the solving the problem in effectively inducing human umbilical cord mesenchymal stem cells to massively synthesize and secrete mesenchymal stem cell exosome capable of specifically promoting epidermis cell and hypodermal cell to proliferate, divide and differentiate, promoting the formation of collagenous fibers, reticular fibers and elastic fibers, and adjusting the secreting functions of collagen and mucopolysaccharide. The cream comprises human umbilical cord mesenchymal stem cell exosome extracting liquid and a base cream formula based on the mass ratio ranging from (1:20) to (3:10), wherein the human umbilical cord mesenchymal stem cell exosome extracting liquid is prepared by performing hungry culture on P2-P5 generations of human umbilical cord mesenchymal stem cells and stimulating through epidermal growth factors. The prepared cream has the effects of improving the skin quality, resisting the aging of skin cells, recovering the normal structure and physiological function of the skin, reducing the skin atrophy exsiccosis, and moisturizing the skin tissue.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a human umbilical cord mesenchymal stem cell exosome cream and a preparation method thereof. Background technique [0002] With the gradual improvement of people's material living standards, the affluence of life makes people begin to pursue a higher level of life. Everyone has a love for beauty, and everyone hopes to be young and beautiful forever. As the largest tissue and organ of the human body, the skin covers the appearance of the human body and is the main tissue and organ of aesthetic beauty research. [0003] How to delay and prevent the aging of skin cells is not only the fundamental issue of beauty and anti-aging, but also the key to skin care. With age, the ability of basal cells to divide gradually decreases, the level of skin growth factors decreases, and the effect weakens, apoptosis increases with age, fibroblasts gradually decrease, and the collagen secreted by fibrobl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/36A61K8/64A61K8/65A61K8/67A61K8/73A61Q19/00A61Q19/08
CPCA61K8/36A61K8/64A61K8/65A61K8/673A61K8/735A61K8/99A61K2800/805A61Q19/00A61Q19/08
Inventor 祁守岗胡建华王晶晶程琴
Owner 南京九圣生物医学科技股份有限公司
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