13 results about "Collagen fibril" patented technology

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

A system for visualizing a target site in an eye using a polarization sensitive optical coherence tomography (PS-OCT) device includes a controller having at least one processor and at least one non-transitory tangible memory on which instructions are recorded. The target site is one or more vitreous opacity in vitreous fluid of the eye. The controller is configured to receive the PS-OCT data and determine, based on the PS-OCT data, at least one parameter corresponding to a birefringence characteristic of collagen fibrils in the vitreous humor. The at least one parameter includes a respective pitch of collagen fibrils. The controller is configured to determine a respective location of the one or more vitreous opacity when the at least one parameter exceeds a predefined range, and to generate a control signal adapted to direct the treatment beam at the respective location of the one or more vitreous opacity.

This invention relates to the field of congenital heart disease treatment technology, and discloses the application of sapropterin in the prevention and treatment of bisphenol fluorene-induced congenital heart disease in fetuses. The congenital heart disease includes cardiac structural defects, heart failure, or cardiomyocyte lesions, specifically selected from ventricular septal defects, atrial septal defects, tetralogy of Fallot, myocardial hypertrophy, decreased cardiac ejection fraction, decreased short-axis contraction rate, cardiomyocyte ferroptosis, myocardial tissue inflammatory infiltration, or myocardial collagen fibrosis. Sapropterin is used as a direct supplement to biological tetrahydropterin (BH4). Through exogenous intervention, it precisely reverses the endogenous BH4 deficiency caused by bisphenol fluorene exposure, restores the expression of GTP cyclase 1 (GCH1) and the dynamic balance of RNA m6A, and upregulates the level of YTH domain family protein 2 (YTHDF2), thereby blocking ferroptosis signal transduction, reducing oxidative stress and inflammatory response, and inhibiting the process of myocardial tissue fibrosis at the molecular level.

The invention discloses application of overexpressed AQP1 mitochondria in preparation of products for treating bone defect, stem cells of overexpressed AQP1 are constructed through a lentiviral transfection method, overexpressed AQP1 mitochondria is obtained, and the obtained mitochondria has mitochondrial structure and function integrity and is enriched with AQP1 protein; in an in-vitro experiment, the overexpressed AQP1 mitochondria can inhibit the polarization and osteoclast differentiation capability of macrophages and relieve inflammation; a periodontitis animal model research shows that overexpression AQP1 mitochondrial transplantation can regulate and control a bone immune microenvironment and relieve tissue inflammation damage; the bone resorption can be inhibited, the damage of tissue collagen fibers is reduced, and the bone defect repair and regeneration of diseases such as periodontitis are promoted. Therefore, the overexpressed AQP1 mitochondria can be used for precise treatment of diseases such as periodontal tissue or bone tissue defects, the treatment effect is remarkably superior to that of common mitochondria, and the overexpressed AQP1 mitochondria has good transformation application prospects in the fields of tissue engineering and cell-free treatment.

The present technique pertains to a method of preparation of a succinylated collagen-fibrinogen hydrogel and a succinylated collagen-fibrinogen hydrogel prepared thereby. The method of preparation of a succinylated collagen-fibrinogen hydrogel according to the present technique can produce a collagen-based bio-substance in a simple process, wherein collagen can be prevented from being entangled and an improvement can be brought about in hydrophilicity, cell growth rate, cell compatibility, and cell diffusion capability, whereby the collagen-based bio-substance exhibits excellent biocompatibility in vivo.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to application of herba artemisiae scopariae and an ethanol herba artemisiae scopariae extract in a medicine for treating autoimmune hepatitis, the ethanol herba artemisiae scopariae extract can reduce ALT and AST of a patient suffering from the autoimmune hepatitis, can effectively reverse liver tissue lesions of the patient suffering from the autoimmune hepatitis, and has the advantages that the curative effect is good, and the curative effect is good. The content of collagen fibrinogen in animal livers is reduced, so that hepatic fibrosis of patients with the autoimmune hepatitis is effectively reversed, proinflammatory factors TNF-alpha, IL-6 and IL-1beta in serum are reduced, the content of an anti-inflammatory factor IL-10 is increased, and the composition has a protective effect on inflammatory injury of liver tissues with the autoimmune hepatitis and can be used for treating the liver with the autoimmune hepatitis. And the content of serum immune globulins IgG and IgM can also be reduced. Through the effects, the oriental wormwood and the ethanol oriental wormwood extract can effectively improve autoimmune hepatitis.

The invention relates to a method for preparing a tensile test on an elongate, more particularly fibrous, specimen, for example on a collagen fibril, comprising the steps of:—providing the elongate specimen;—attaching a handling particle to the elongate specimen;—providing a force sensor, on which a retainer for the handling particle on the elongate specimen is disposed;—connecting a handling apparatus to the handling particle on the elongate specimen; and—connecting the handling particle on the elongate specimen to the retainer on the force sensor by means of the handling apparatus. The invention also relates to a method and a device for performing a tensile test on an elongate specimen.

The invention relates to a composition capable of intervening collagen fibrosis and a preparation method and application thereof.The composition comprises gallic acid or gallic acid derivatives, and when the composition is used for repairing damaged skin, fibroblast differentiation can be intervened; the composition can also comprise silicone and Ectoine, and the three components cooperate to interfere with collagen fibrosis, so that the composition can be further used for treating or preventing skin diseases such as scars, scleroderma or skin photoaging caused by collagen fibrosis.

The present invention discloses a type I collagen fibril with highly bone-mimicking mineralization and a preparation method thereof. The preparation method is to dissolve collagen in an acid solution to prepare a collagen acid solution, and then successively add a calcium salt solution and a phosphate solution dropwise into the collagen acid solution for thorough mixing, with continuous stirring and a certain temperature condition throughout the process. Then, under the condition of maintaining stirring, the pH is adjusted, and then the stirring is stopped and the mixture is allowed to stand. The precipitated part is separated, washed, and dried to obtain a type I collagen fibril with highly bone-mimicking mineralization. This preparation method realizes uniform, continuous, and sufficient mineralization both inside and on the outer surface of the collagen fibril, achieving a high degree of biomimicry of the basic unit of natural bone tissue, namely its secondary microstructure (bone-mineralized collagen fibril). The collagen fibril prepared by this preparation method can provide a simulated three-dimensional scaffold microenvironment for osteoblasts and supply calcium ions and phosphate ions to promote bone regeneration and repair.

The invention relates to the technical field of biomimetic materials, in particular to a multiphase biomimetic mineralized collagen scaffold as well as a preparation method and application thereof. The preparation method comprises the following steps: self-assembling an I-type collagen solution and solid-phase copper sulphide nanoparticles, and carrying out intra-fiber mineralization on collagen fibrils by virtue of a liquid-phase mineralization technology, so as to form the multiphase biomimetic mineralized collagen scaffold which is similar to a natural bone nanostructure and Young modulus. The scaffold can improve energy metabolism of cells by regulating oxidative phosphorylation of bone marrow mesenchymal stem cells, so as to promote osteogenic differentiation of the bone marrow mesenchymal stem cells; in addition, copper ions are released through the stent to play the activity of mimic enzyme, and a large amount of active oxygen is generated in bacteria through catalysis, so that membrane rupture and content overflow of the bacteria are caused, and the antibacterial effect is achieved. The scaffold can be used for treating simple bone defects or infectious bone defects by regulating energy metabolism of cells and exerting mimic enzyme activity, and more novel methods for realizing bone regeneration and preparing anti-infection drugs are provided for clinic.

Products, processes, compositions, kits, and methods are provided for cartilage-derived implants. The implants can exhibit resistance to enzyme (e.g., collagenase, protease, etc.) digestion compared to the source tissue from which they were derived while still having one or more mechanical properties comparable to the source tissue from which they were derived. The implants can also have a plurality of molecular bridges between molecules of the cartilaginous material. The molecular bridges can connect one or more collagen fibrils and / or / with one or more glycosaminoglycans. The implants can also be treated with cationic detergent, packaged and sterilized with or without additional components, and surgically implanted into subjects.

The invention discloses collagen-fibrin composite gel for cartilage defect repair and a preparation method of the collagen-fibrin composite gel, and belongs to the technical field of gel preparation. The preparation method of the collagen-fibrin composite gel comprises the following steps: mixing a collagen solution with a thrombin solution to obtain a collagen-thrombin mixed solution; preparing a fibrinogen solution; and respectively filling the collagen-thrombin mixed solution and the fibrinogen solution into a duplex syringe, synchronously extruding and mixing to obtain a composite gel precursor, and standing to obtain the collagen-fibrin composite gel. The prepared gel has good mechanical properties, can induce bone marrow mesenchymal stem cells and surrounding autologous cartilage cells to migrate inwards, enables the cells to be amplified according to the three-dimensional structure of the gel, and further can be used as a raw material for repairing defective cartilage to achieve repairing of the defective cartilage.