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Collagen and extraction method thereof

A collagen and extraction method technology, applied in the field of medical biology, can solve the problems of high extraction rate, large amount of raw materials used, destruction of collagen molecular structure, etc., and achieve the effect of efficient extraction

Pending Publication Date: 2019-05-24
WUHAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a natural protein, the integrity of the triple helix structure of natural collagen is crucial to the application of collagen. There are still many shortcomings in the current extraction technology, such as the large amount of raw materials used and the low extraction rate of collagen, or the high extraction rate and the molecular structure of collagen Therefore, it is of great practical significance to seek a simple and convenient extraction method that can retain the triple helix structure of collagen

Method used

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  • Collagen and extraction method thereof
  • Collagen and extraction method thereof
  • Collagen and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Remove the hair and impurities from the fresh pigskin, degrease and remove impurities, and air-dry at low temperature for later use.

[0046] Freeze the air-dried degreased pigskin in liquid nitrogen for 24 hours and then take it out, weigh a certain quality of pigskin sample, chop it up, and transfer it to a cleaned 25mL grinding tank (containing two After the grinding tank is locked and sealed, the grinding tank is clamped on the grinder, and it is ground at a fixed frequency of 25Hz for 3 minutes to obtain a uniform powder sample, and then the pigskin collagen is extracted by the traditional acid / enzyme method Protein, the steps include: acid extraction: soak the ground sample with 0.5mol / L acetic acid (the ratio of solid to liquid is 1:20, that is, 1g of the ground sample is dissolved in 20mL of acetic acid with a concentration of 0.5mol / L) and mechanically stir for 4d (carried out in a low-temperature operating table), add pepsin to make the concentration of pepsi...

Embodiment 2

[0050] The difference from Example 1 is that the time for grinding the defatted pigskin is 5 minutes.

Embodiment 3

[0052] The difference from Example 1 is that the time for grinding the defatted pigskin is 10 minutes.

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Abstract

The invention discloses collagen and an extraction method thereof. The method includes the steps: 1) refrigerating and rubbing animal tissues containing the collagen in liquid nitrogen; 2) extractingthe collagen from the rubbed animal tissues by an acid / enzyme method to obtain collagen solution; 3) dialyzing and freeze-drying the collagen solution to obtain the powdery collagen. By the refrigeration-rubbing pretreatment method, the animal tissues containing the collagen is refrigerated in the liquid nitrogen in advance for a period of time and rubbed into powder under a certain frequency by arubbing instrument, a triple-helical structure and thermal denaturation temperature cannot be affected after treatment by the method, compared with an original acid-extraction method without refrigeration-rubbing, the extraction rate of the method is increased by 18%-82%, and the method is of great significance for application of the collagen and collagen-based biological materials.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and more specifically relates to a collagen and an extraction method thereof. Background technique [0002] Collagen is the most abundant structural protein in animals, widely distributed in tissues and organs such as bones, cartilage, tendons, skin, intima, and blood vessels. Among the 28 known collagen types, type I collagen is the most abundant and widely distributed. A typical type I collagen molecule consists of two α1 and one α2 chains, forming a rope-like triple helix structure with a molecular weight of about 300kDa. Collagen has excellent biocompatibility, suitable degradability, good biomechanical properties, etc. As a natural biological framework component, it has huge application space in clinical medicine, tissue engineering, medical bioengineering and other fields. With the rapid development of molecular biology, collagen has become the most promising green renewable material....

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/30C07K1/14
Inventor 徐玉玲汪海波何浪李盛张军涛未本美
Owner WUHAN POLYTECHNIC UNIVERSITY
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