35 results about "Thermal denaturation" patented technology

The invention discloses a method for separating and purifying gamma-aminobutyric acid from whole cells or enzymatic conversion liquid. The method comprises the following steps: removing thalli and large-particle impurities, denaturating, decolorizing and removing impurities, crystallizing for the first time, washing for the first time, crystallizing for the second time, washing for the second time and drying. According to the present invention, the thermal denaturation of the protein, the active carbon decoloration and the flocculation impurity removal are integrated, such that the solid-liquid separation frequency is reduced, the problems of difficult filtration and easy leakage of the powdered active carbon are solved, and the purity and the yield of the gamma-aminobutyric acid are significantly improved. In the whole separation and purification process, the pH value is not adjusted, the cost is saved, impurities are reduced, and the ion removal process is omitted. And collecting the primary crystallization mother liquor and washing liquor for secondary crystallization, so that the yield of the final product is greatly improved. According to the separation and purification method, the purity of the obtained gamma-aminobutyric acid is greater than 99%, the yield is greater than 92%, and the crystal light transmittance is greater than 99%. The method provided by the invention is simple, efficient and low in cost, and has a wide application prospect.

The invention relates to the technical field of bioengineering, in particular to an SOD (superoxide dismutase) impurity removal and purification process which comprises the following steps: extracting SOD enzyme from fresh animal blood, separating high-purity red blood cells through gradient centrifugation, preparing a crude extract by combining hypotonic lysis and freeze thawing strengthening, then selectively precipitating hemoglobin and other heat-sensitive impurity proteins by adopting a gradient temperature rise thermal denaturation method, and purifying to obtain the SOD impurity removal and purification process. The method comprises the following steps: extracting SOD, removing pigments and lipid impurities by using a mixed adsorbent, further enriching SOD by using ammonium sulfate salting-out, and finally obtaining high-purity SOD powder through vacuum freeze drying by combining ion exchange chromatography and ultrafiltration concentration technologies. The method has the advantages of high efficiency, rapidness and low cost, impurities can be effectively removed through a combined process of hypotonic / freeze-thaw cracking, hemoglobin removal through thermal denaturation, ammonium sulfate salting-out, ion exchange chromatography and ultrafiltration concentration, the purity and the recovery rate of the SOD enzyme are remarkably improved, the whole purification process is carried out at 4 DEG C, and the method is suitable for industrial production. The influence of high temperature on the activity of the SOD enzyme is avoided.

The invention discloses a biosensor based on an ultraviolet light triggered bio-barcode release technology (OBCA) and construction and application of the biosensor. The biosensor comprises nucleic acid functionalized silver nanoparticles, nucleic acid functionalized magnetic beads and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a detection system, the DNA bar code modified on the surface of the silver nanoparticle contains a light cleavable linker, a CRISPR / Cas12a activation sequence and a target capture sequence I, and a DNA capture probe containing a target capture sequence II is fixed on the surface of the magnetic bead through biotin-streptavidin action. During detection, target miRNA mediates two capture sequences to form a sandwich compound, after magnetic separation and thermal denaturation elution, 365nm ultraviolet radiation releases DNA bar code fragments, and the Cas12a trans-cleavage fluorescence quenching reporter probe is activated to generate a signal. According to the method, nucleic acid amplification and reverse transcription are not needed, the detection limits of miR-21, miR-155 and mi-210 respectively reach 158.94 aM, 763.56 aM and 197.82 aM, single-base mismatch and precursor miRNA can be distinguished, the serum sample detection result is highly consistent with that of RT-PCR, and the method is suitable for high-sensitivity quantitative detection of miRNA.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

The invention relates to the technical field of biomedical materials, in particular to polynucleotide / macromolecular protein composite gel capable of tolerating terminal moist heat sterilization as well as a preparation method and application of the polynucleotide / macromolecular protein composite gel. The product provided by the invention is a composite gel of polynucleotide PN and a specific type of high-molecular protein, and under the conditions of a specific ratio and molecular weight, the high-thermal denaturation temperature is higher, and the high-molecular protein with a specific structure has a better protection effect on the polynucleotide PN; the stability and biological activity of polynucleotide PN molecules can be kept under the condition of high-temperature terminal sterilization, the safety of a sterilized product can achieve the effect of thoroughly inactivating viruses, and the filling and supporting properties of the product serving as a stent material are completely reserved; the compound can be directly used in drugs, medical instruments or cosmetics in the field of tissue engineering, and has high safety and lasting effect.

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

The invention discloses differential temperature comparison single molecule detection based on a reversible thermal change renaturation query probe, and relates to the field of single molecule immunodiagnosis. According to the invention, the property of repeated thermal denaturation / renaturation of the query probe is utilized, and the specific binding activity of the query probe is controlled, so that a specific binding molecular signal and a non-specific binding molecular signal of the query probe are distinguished, and a novel high-specificity single-molecule immunodiagnosis method is provided.

This application discloses an ultrasonic thermal strain measurement method and system based on equivalent thermal dose correction, relating to the field of thermotherapy monitoring: acquiring the initial temperature of biological tissue; during the heating process of biological tissue, acquiring ultrasonic echo signal sequences of the target area at preset time intervals; calculating the cumulative thermal strain of the target area at each measurement moment based on the ultrasonic echo signal sequence; coupling and predicting the temperature and equivalent thermal dose at the current moment based on the equivalent thermal dose and temperature at the previous measurement moment, obtaining the predicted values ​​of the temperature and equivalent thermal dose at the current moment; establishing a temperature inversion equation including cumulative thermal strain and equivalent thermal dose, iteratively solving the equation using the predicted value as the initial value, and outputting the corrected temperature value and updated equivalent thermal dose value at the current moment. This application achieves dynamic compensation for the nonlinear changes in acoustic parameters caused by tissue thermal denaturation, thereby improving the temperature measurement accuracy during the ablation stage, expanding the temperature measurement range, and realizing real-time synchronous monitoring of temperature and equivalent thermal dose.

Disclosed are MHC Class II constructs (CIICs) comprising DQ and DR gene products that can present peptide epitopes associated with cancers, allergies, autoimmune diseases (e.g., T1D and celiac disease), GVHD, HGVD, and infections to T cell receptors. The CIICs may also comprise sequences of immunomodulatory molecules (MODs) such as IL-2 or PD-L1 that can modulate receptors on the surface of T cells. CIICs are expressible at levels up to about 350 mg / I in culture, and are substantially stable to multiple freeze thaw cycles and to thermal denaturation at 42° C. The stability of CIICs and their ability to present peptide epitopes and MODs to T cells and their renders them useful as therapeutics for in vitro and in vivo methods of treating various cancers, allergies, autoimmune diseases (e.g., T1D and celiac disease), GVHD, HGVD, and infections.

A medical device includes a processor including hardware, the processor being configured to acquire setting information in which a region of interest is set for a biological tissue and thermal denaturation information regarding a thermally denatured region in which thermal denaturation has occurred by heat treatment for the biological tissue, determine whether or not there is the thermally denatured region outside the region of interest based on the setting information and the thermal denaturation information, and output support information indicating that there is the thermally denatured region outside the region of interest when it is determined that there is the thermally denatured region outside the region of interest.

This invention relates to the field of biodetection technology, specifically to a non-isolated digital multiplex detection method based on a DNA walker and TdT enzyme amplification. The method first utilizes coding microspheres with capture elements coupled to their surface to specifically bind to target miRNAs or proteins in the sample; then, specific guide DNA (gDNA) corresponding to the target molecule is released through enzymatic digestion or thermal denaturation; the gDNA then... Pf Under the action of Ago endonuclease, the DNA walker on the surface of the encoded microspheres is activated, cyclically cutting the orbital strand to generate multiple DNA fragments with 3'-hydroxyl ends. Finally, TdT enzyme is used to extend fluorescently labeled nucleotides at the hydroxyl ends, achieving in-situ signal amplification, and digital quantitative analysis is performed on the fluorescence signal of the encoded microspheres. This invention achieves high-sensitivity detection of multiple targets in a single reaction without chamber isolation, with detection limits for each target molecule reaching the fM or fg / mL level, providing an effective technical means for the early screening of major diseases such as Alzheimer's.

The invention belongs to the technical field of bioactive protein peptides / protein hydrolysates, and particularly relates to an anti-aging soybean protein peptide and a preparation method thereof. Soybean protein is used as a raw material, the soybean protein is firstly subjected to thermal denaturation treatment to change the secondary structure of the soybean protein, and then the alkaline subunit amount of the thermal denaturation soybean protein is increased by utilizing high static pressure physical treatment, so that the exposure of hydrophobic groups in soybean protein molecules and the increase of enzyme action sites are facilitated, and the proteolysis rate is further increased. And finally, carrying out enzymolysis on the treated soybean protein by using alkaline protease to obtain the soybean protein peptide. The soybean protein peptide obtained by the preparation method disclosed by the invention has very strong antioxidant activity and a good anti-aging effect.

The medical device (4) is provided with processors (406, 409) that process captured images in which fluorescent light generated from biological tissue by irradiating the biological tissue with excitation light has been captured. A processor (406, 409) performs: a process for acquiring a tissue image including a site to be thermally treated in biological tissue; acquiring range information indicating a treatment target range of thermal treatment in the tissue image; determining a state of thermal denaturation caused by the thermal treatment on the basis of the captured image; and causing a notification unit (3) to notify thermal denaturation information indicating the state of thermal denaturation within the treatment target range.

A medical device (4) is provided with a processor (409) for processing a captured image in which fluorescence generated from biological tissue by irradiating the biological tissue with excitation light has been captured. The processor (409) determines a change in the state of the thermal denaturation on the basis of the captured image, and, on the basis of the determination result of the change in the state of the thermal denaturation, transmits a control signal for controlling the operation of the perfusion device (6) to the perfusion device (6) that perfuses the perfusion liquid.

Provided are a medical device, a medical system, a learning device, a method for operating a medical device, and a program with which it is possible to confirm the state of thermal denaturation in a specific region. A medical device is provided with a processor that performs: a process for acquiring a thermally denatured image including at least a thermally denatured region; determining a specific area contained in the thermal denaturation image; setting a detection range, which is a target range in which a thermally denatured region is detected, in the thermally denatured image on the basis of a specific region; and outputting thermal denaturation information based on the detection range and the thermal denaturation region.

The present application discloses a method for analyzing the interaction between strigolactone hormones and receptor proteins based on mass spectrometry and Tycho instrument, which relates to the field of biotechnology and includes the following steps: Step S1: Incubate the receptor protein solution with the strigolactone compound solution or the blank solvent to obtain the test incubation solution and the blank incubation solution respectively; Step S2: Detect the test incubation solution and the blank incubation solution respectively by the Tycho instrument to obtain the thermal denaturation curve of the test incubation solution and the thermal denaturation curve of the blank incubation solution; Step S3: Analyze the test incubation solution and the blank incubation solution respectively using mass spectrometry to obtain the mass spectrometry of the test incubation solution and the mass spectrometry of the blank incubation solution. By comparing the peak shift of the thermal denaturation curve and the difference in protein signals in the mass spectrometry Δ m / z to determine whether the strigolactone hormone interacts with the receptor protein and whether a hydrolysis reaction occurs. This method can not only be used for the detection of the interaction between plant hormones and receptors, but also for the discovery of target proteins of natural active small molecules.

A medical device includes a processor configured to acquire a first image that includes layer information of a biological tissue including a plurality of layers, acquire a second image that is a fluorescence image and includes thermal denaturation information regarding thermal denaturation caused by heat treatment on the biological tissue, acquire correlation information indicating a preset relationship between an emission intensity and a depth of thermal denaturation from a surface layer in the biological tissue, determine a presence or absence of the thermal denaturation of a predetermined layer in the biological tissue based on the first image and the second image, determine a depth of thermal denaturation of the predetermined layer from the surface layer in the biological tissue based on an emission intensity of the fluorescence image and the correlation information, and output depth information regarding the determined depth.

Provided are a medical device, a medical system, a method for operating a medical device, and a program with which it is possible to confirm the state of coagulation degeneration with respect to blood vessels. This medical device is provided with a processor that performs: a process for acquiring a display image in which a blood vessel region of a blood vessel in a subject is identified, and a fluorescence image that overlaps at least a part of a field-of-view region of the display image; determining thermal denaturation information in the blood vessel region on the basis of the display image and the fluorescence image; and outputting the thermal denaturation information.

A method for determining or verifying the identity of a protein based on a melting curve obtained from a nano-differential scanning fluorimeter (nanoDSF). The method uses a machine learning model trained on a set of proteins of known identity and includes acquiring, through use of nanoDSF, a set of data points representative of a thermal denaturation curve (melting curve) of a test protein, generating a set of features derived from the melting curve, and providing the set of features as inputs to the trained machine learning model to obtain a corresponding output indicative of an identity of the test protein. Methods for training the machine learning model are provided, as well as systems including processor-executable instructions that, when executed by a processor, cause the system to perform the method for determining or verifying protein identity.

The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and / or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The application discloses a preparation and separation and purification method of deer antler peptide by directional enzymolysis, and belongs to the technical field of bioactive peptide preparation. Deer antler collagen is subjected to mechanical energy auxiliary treatment in an alkaline medium under low-temperature conditions lower than the thermal denaturation temperature of the deer antler collagen, so that the triple helix structure is reversibly unfolded to increase the accessible surface area of the protease and the conformation of the active segment of the ginseng protein is retained. The treated deer antler collagen and the ginseng protein are prepared into the same substrate solution and subjected to synergistic enzymolysis. Alkaline protease, collagenase and aminopeptidase are used for directional enzymolysis in steps, so that the product is directionally enriched with two characteristic dipeptides, prolyl-hydroxyproline and hydroxyprolyl-glycine. The dipeptides are separated by three steps of ultrafiltration, gradient elution of a macroporous resin and reverse phase preparative chromatography in series, so that the low-temperature mechanical energy reversible unfolding replaces the high-temperature pre-denaturation, the contradiction between the two types of raw materials in the treatment temperature window is eliminated, the two types of raw materials can be synergistically treated in the same system, and the dipeptide yield is higher than that of the separate enzymolysis.

The invention relates to the technical field of collagen oligopeptides, in particular to a collagen oligopeptide and an extraction process thereof. The collagen oligopeptide provided by the invention overcomes the problems of poor water retention capacity and poor antioxidant activity of traditional collagen oligopeptide. Pretreatment of the fish skin and the fish bone is combined with critical thermal denaturation treatment; the collagen oligopeptide is endowed with high water holding rate, high moisture absorption rate and high water retention capacity by optimizing the ratio of various substances in a mixing and homogenizing process and combining step-by-step enzymolysis to preliminarily screen the oligopeptide meeting the requirements; through the synergistic effect of step-by-step enzymolysis and ultrafiltration treatment, 'directional enzymolysis-active peptide enrichment-molecular weight accurate control 'is realized; the collagen oligopeptide with a porous freeze-drying network is formed through the synergistic effect of nanofiltration treatment and post-modification treatment, and the oxidation resistance of the collagen oligopeptide is improved.

The invention provides an octagonal duck meat marinating parameter detection, regulation and control system, relates to the technical field of food production equipment, and solves the problem that a traditional model is difficult to consider the oily degree and the tasty depth of duck meat by constructing a two-dimensional dynamic marinating state space containing a fluid shear resistance characteristic entropy and a system reheating impedance phase angle. The fluid characteristics and the muscle fiber thermal denaturation degree are solved by utilizing motor current harmonic analysis and thermal impulse response cross-correlation analysis, so that non-intrusive accurate judgment on the marinating liquid emulsification grade and the duck meat curing stage is realized; aiming at the working condition of heat retardation, the cooperative closed-loop regulation and control module reduces the viscosity by improving the frequency of a circulating pump and utilizing the shear thinning characteristic, and limits the heating duty ratio to prevent coking, so that the limitation of optical and contact type detection under the severe working condition is overcome, the taste and flavor of marinated duck meat are ensured, and the whole-process self-adaptive closed-loop regulation and control are realized.

The invention provides an amplification library building method for high-throughput sequencing verification of synthetic genes. The amplification library building method comprises the following steps: obtaining a joint connection product of which two ends are connected with double-chain joints after a to-be-detected synthetic gene sequence, and carrying out first PCR (Polymerase Chain Reaction) amplification by adopting a first gene specific primer by taking the joint connection product as a template to obtain a first amplification product; taking the first amplification product as a template, and carrying out second PCR amplification by adopting a second gene specific primer to obtain a second amplification product; performing thermal denaturation single-chain separation and single-chain cyclization on the second amplification product to obtain single-chain circular DNA; the preparation method comprises the following steps: carrying out DNB preparation on single-stranded circular DNA to obtain DNB nanospheres; and sequencing the DNB nanospheres to obtain synthetic gene sequence information. The method verifies that the synthesized gene is high in flux, and the flux requirement of large-scale gene synthesis can be met.

The invention relates to a preparation method of high-heat-resistance ferritin, and relates to the field of food processing. A salt bridge network structure is added on a ferritin C3 interface, so that the thermal denaturation temperature of the ferritin is remarkably improved. In the aspect of application, due to the high thermal stability of the mutant ferritin, the mutant ferritin has a more remarkable protection effect on the loaded functional factors at high temperature, which is of great significance to the application of the functional factors in the food processing industry.

The invention discloses a shark source single-domain antibody targeting LRP6 and application of the shark source single-domain antibody. The single-domain antibody has an amino acid sequence as shown in SEQ ID NO: 1, shows nanomole-level high affinity to LRP6 protein, has an affinity constant KD value of 1.8 * 10 <-9 > M, shows excellent physical and chemical stability, has a thermal denaturation temperature as high as 75.6 DEG C, and can still retain 92% or more of binding activity after being incubated for 2 hours under a strong acidic condition. Meanwhile, the antibody has high specificity, and the cross reaction rate of the antibody with homologous protein LRP5 and homologous protein LDLR is lower than 4%. The invention also provides a composition for reducing hypertension, hyperglycemia and hyperlipidemia, which contains the single-domain antibody and a specific natural plant extract composite component. Experiments show that the composition can synergistically act on a plurality of pathological links of the metabolic syndrome, the symptoms of hyperglycemia, hyperlipidemia and hypertension are remarkably improved in an animal model, the effect is better than that of a single component and a traditional control drug, and a brand new multi-target treatment scheme is provided for prevention and treatment of the metabolic syndrome.

The application relates to the technical field of preparation of canine interferon, and particularly provides a preparation method and a preparation device of canine interferon. The preparation device of the canine interferon comprises a frame body assembly, a barrel body assembly, a driving assembly, a homogenate synchronization assembly and a homogenate assembly. The application can realize controllable grading crushing, guarantee sufficient crushing of bacteria bodies, complete release of products, improve crushing efficiency, avoid excessive crushing to cause fine crushing of the bacteria bodies, effectively absorb instantaneous impact force generated by material impact, avoid excessive crushing, reduce abrasion and vibration of the equipment, provide uniform and stable temperature control for the barrel body assembly, effectively guarantee the biological activity and purity of the products. In addition, IPTG inducer is used in cooperation with low-temperature constant-temperature induction to guarantee stable and high expression of target proteins, reduce the risk of protein thermal denaturation, reduce the difficulty of inclusion body treatment, and use multi-step chromatography purification to guarantee product quality and long-term stability of the products.

Provided are a medical device, a medical system, a learning device, a method for operating a medical device, and a program with which it is possible to confirm the depth of biological tissue to which thermal denaturation has reached. This medical device is provided with a processor that performs: a process for acquiring a first image that includes layer information of a biological tissue comprising a plurality of layers, and a second image that includes thermal denaturation information relating to thermal denaturation caused by thermal treatment of the biological tissue; determining whether there is thermal denaturation reaching a predetermined layer in the biological tissue on the basis of the first image and the second image; and outputting support information indicating thermal denaturation reaching a predetermined layer on the basis of the determination result of determining whether thermal denaturation exists or not.

A purpose of the present invention is to provide a recombinant expression technique for producing a protein glutaminase in which decreases in thermostability are suppressed. A complex protein glutaminase according to the present invention is a thrombin cleavage product of a mutant propolypeptide comprising a propolypeptide sequence which includes a pro-sequence and a mature sequence of protein glutaminase, wherein a thrombin recognition sequence is introduced into a C-terminal region of the pro-sequence. The complex protein glutaminase includes: (A) a pro-sequence peptide having an N-terminal cleavage fragment of the thrombin recognition sequence at the C-terminal thereof; and (B) a mature protein glutaminase having a C-terminal cleavage fragment of the thrombin recognition sequence at an N-terminal thereof. Said complex protein glutaminase has an improved thermal denaturation temperature compared to non-complex protein glutaminases.

The invention discloses a PCR (Polymerase Chain Reaction) method for detecting Lawsonia intracellular bacteria in excrement, which is characterized in that a Lawsonia intracellular bacteria detection method is optimized through primer screening, concentration dilution, different thermal denaturation procedures and high-speed centrifugation selection, and the PCR method which is most suitable for detecting Lawsonia intracellular bacteria in a common pig farm is selected. The method disclosed by the invention only needs simple direct dilution and centrifugation, is simple to operate, low in technical requirements on operators, high in repeatability, economical in scheme, simple to operate, low in instrument requirements and suitable for detection of Lawsonia intracellular bacteria in excrement of a common pig farm.