Method for extracting hemoglobin and superoxide dismutase from livestock blood

Abstract

The invention discloses a method for extracting hemoy lobin and superoxide dismutase from bloods of livestock, which comprises the steps that: after going through an anti-freezing treatment, the fresh bloods of livestock is centrifuged to collect corpuscles, and after hemolysis, heating treatment is carried out so as to extract the hemoy lobin and SOD crude extracted liquid; SOD refined products are obtained after the SOD crude extracted liquid goes through treatments of thermal denaturation, super-filtration concentration, fractionated precipitation of acetone and absorption by ion exchanger. The method of the invention has simple operation and low production cost, is suitable for large-scale industrialized production, and can be used for simultaneously extracting the SOD and hemoy lobin in the bloods of livestock, thereby achieving the highly effective recovery of a plurality of protein resources from the blood of livestock. The extracted hemoy lobin contains salts such as sodium chloride and potassium chloride, etc which are harmless to the human body without containing copper ions. Therefore, the extracted hemoy lobin can be served as food for human beings and livestock, and also can be used for further producing hemoy lobin products such as hemachrome and decolored globin, etc.

Description

technical field

[0001] The invention relates to a method for extracting hemoglobin and superoxide dismutase from poultry blood, and belongs to the fields of deep processing of agricultural and sideline products and production of biochemical products. Background technique

[0002] Poultry blood is rich in nutrients, and its protein content is as high as about 20%. It is known as "liquid meat". Poultry blood can be centrifuged to obtain plasma and blood cells. Plasma mainly contains albumin, and blood cells contain hemoglobin, superoxide dismutase (SOD) and catalase, etc., of which hemoglobin accounts for more than 90% of the total hemoglobin protein , SOD accounts for 0.3% to 0.5%. SOD can specifically remove superoxide free radicals in the body, reduce the oxidative stress of the body, and have various physiological effects such as anti-radiation, anti-aging, and anti-inflammatory. SOD is widely used in health care products, cosmetics and medicines, especially Dabao SOD, w...