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Method for extracting hemoglobin and superoxide dismutase from livestock blood

A technology of superoxide and hemoglobin, which is applied in the preparation methods of peptides, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of incapability of large-scale production, inability to be reused, waste of protein resources, etc., and achieve production Low cost, high repeatability, simple and convenient operation

Inactive Publication Date: 2009-01-28
抚顺丹阳生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two methods for extracting SOD. One method uses organic solvents to extract SOD. This method has relatively small processing capacity, high production costs, and cannot be produced on a large scale; the other method uses copper salts to precipitate hemoglobin to purify SOD. This method generates The hemoglobin contains a lot of copper ions, which cannot be reused, wasting a lot of precious protein resources, and the discharge of these proteins will also cause environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take one ton of fresh pig blood from the slaughterhouse, add pre-dissolved 3.33kg of sodium citrate trihydrate, 1.2kg of citric acid, and 5kg of glucose for anticoagulant treatment, centrifuge to obtain 400kg of blood cells, add 800kg of water and stir for 1 hour, adjust the pH with hydrochloric acid To 3.0, add 210kg of sodium chloride, stir to dissolve, then slowly heat to 60°C, keep warm for 60min, and press filter. The filter residue was dried to obtain 130kg of hemoglobin. Add 5kg of copper sulfate to the filtrate, stir to dissolve, heat to 75°C for 30 minutes, cool to room temperature, press filter to remove residue, and obtain 1 ton of blue filtrate. The filtrate is concentrated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 20,000. When it is concentrated to about 100kg, add 100kg of water, continue to concentrate by ultrafiltration, repeat five times, and finally concentrate to a volume of 100kg. Add 100kg of 0.2M disodiu...

Embodiment 2

[0022] Take 10kg of fresh duck blood from the slaughterhouse, quickly add 400g of sodium citrate for anticoagulation, centrifuge to obtain 4.5L of blood cells, add 4.5L of water and stir for 1 hour, adjust the pH to 5.5 with hydrochloric acid, add 5g of potassium chloride, stir to dissolve, slowly Heat to 50°C, keep warm for 180min, and press filter. The filter residue was dried to obtain 1.4 kg of hemoglobin. Add 2.25 g of copper sulfate to the filtrate, stir to dissolve, heat to 85°C for 10 minutes, cool to room temperature, press filter to remove residue, and obtain 8 L of blue filtrate. The filtrate is concentrated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 3000. When the concentration reaches 500ml to 800ml, add 800ml of water, continue to concentrate by ultrafiltration, repeat five times, and finally concentrate to a volume of 500ml, add 1.86g of ethylenediaminetetraacetic acid, and stir to dissolve. After adding 1L of water, ...

Embodiment 3

[0024] Take 500kg of fresh beef blood from the slaughterhouse, anticoagulate with potassium oxalate, centrifuge to get 200kg of blood cells, add 1000kg of water and stir for 1 hour, adjust the pH to 3.0 with phosphoric acid, add 700g of iodine-free salt, stir to dissolve, and slowly heat to 60°C. After heat preservation for 180min, filter. The filter residue was dried to obtain 60 kg of hemoglobin. Add 11ml of 1M copper chloride solution to the filtrate, stir evenly, heat to 55°C and keep it warm for 120min, cool to room temperature, press filter to remove residue, and obtain 1100kg of blue filtrate. The filtrate is concentrated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 1000, desalted with the same method as in Example 1, and after removing free copper ions, concentrates to 50kg. Adjust the pH of the concentrated solution to 8.5, add 40L of acetone for pre-cooling at 4°C, stir for half an hour and then centrifuge, take the supernat...

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Abstract

The invention discloses a method for extracting hemoy lobin and superoxide dismutase from bloods of livestock, which comprises the steps that: after going through an anti-freezing treatment, the fresh bloods of livestock is centrifuged to collect corpuscles, and after hemolysis, heating treatment is carried out so as to extract the hemoy lobin and SOD crude extracted liquid; SOD refined products are obtained after the SOD crude extracted liquid goes through treatments of thermal denaturation, super-filtration concentration, fractionated precipitation of acetone and absorption by ion exchanger. The method of the invention has simple operation and low production cost, is suitable for large-scale industrialized production, and can be used for simultaneously extracting the SOD and hemoy lobin in the bloods of livestock, thereby achieving the highly effective recovery of a plurality of protein resources from the blood of livestock. The extracted hemoy lobin contains salts such as sodium chloride and potassium chloride, etc which are harmless to the human body without containing copper ions. Therefore, the extracted hemoy lobin can be served as food for human beings and livestock, and also can be used for further producing hemoy lobin products such as hemachrome and decolored globin, etc.

Description

technical field [0001] The invention relates to a method for extracting hemoglobin and superoxide dismutase from poultry blood, and belongs to the fields of deep processing of agricultural and sideline products and production of biochemical products. Background technique [0002] Poultry blood is rich in nutrients, and its protein content is as high as about 20%. It is known as "liquid meat". Poultry blood can be centrifuged to obtain plasma and blood cells. Plasma mainly contains albumin, and blood cells contain hemoglobin, superoxide dismutase (SOD) and catalase, etc., of which hemoglobin accounts for more than 90% of the total hemoglobin protein , SOD accounts for 0.3% to 0.5%. SOD can specifically remove superoxide free radicals in the body, reduce the oxidative stress of the body, and have various physiological effects such as anti-radiation, anti-aging, and anti-inflammatory. SOD is widely used in health care products, cosmetics and medicines, especially Dabao SOD, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C12N9/08
Inventor 龚兴国郦剑勇叶跃国曾冬云秦勇
Owner 抚顺丹阳生物科技股份有限公司
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