Molecularly-imprinted nanoparticle for glycoprotein and synthesis method thereof

Abstract

The invention discloses a molecularly-imprinted nanoparticle for glycoprotein and a synthesis method thereof, belongs to the field of analytical chemistry, and relates to synthesis of molecularly-imprinted polymers. The synthesis method comprises the following steps: firstly, preparing a solution phase A from template glycoprotein molecules, preparing a solution phase B from a functional monomer and a cross-linking agent, and preparing solution phase C from an initiator; secondly, respectively fixing the solution phase A, the solution phase B and the solution phase C on three micro-fluidic injection pumps with adjustable flow rates, connecting and mixing the solution phase A and the solution phase B through a Y-shaped three-way valve, connecting and mixing the mixed solution and the solution phase C through another Y-shaped three-way valve, and enabling the obtained mixed solution and the solution phase C to flow into a reactor for a polymerization reaction; and finally, collecting a polymerized nanoparticle at the tail end of the reactor, and removing the glycoprotein template molecules to obtain the molecularly-imprinted nanoparticle for glycoprotein. The molecularly-imprinted nanoparticle for glycoprotein is prepared in a microfluidic mode, operation is easy, continuous production can be achieved, and the obtained molecularly-imprinted nanoparticle can be used for specific recognition of target glycoprotein molecules.

Description

technical field

[0001] The invention belongs to the field of analytical chemistry and relates to the synthesis of molecularly imprinted nanoparticles, in particular to a glycoprotein molecularly imprinted nanoparticle and a synthesis method thereof. Background technique

[0002] Molecularly Imprinted Polymer (MIP) is a high molecular polymer with special molecular recognition ability. The formation principle of its selective recognition ability is: firstly, the template molecule and the functional Covalently interact to form a complex, then add a cross-linking agent and an initiator to obtain a molecularly imprinted pre-polymerization solution; then, initiate a polymerization reaction to obtain a high molecular weight polymer containing template molecules; finally, remove the template molecules in the polymer, so that in An imprinted cavity complementary to the shape of the template molecule will be left in the polymer, and the functional group of the functional monomer re...

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