Molecularly-imprinted nanoparticle for glycoprotein and synthesis method thereof

A nanoparticle and molecular imprinting technology, applied in the field of analytical chemistry, which can solve the problems of high polymerization reaction type and conditions, uneven diffusion, and particle agglomeration.

Active Publication Date: 2021-04-20
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]Technical problems to be solved: Aiming at the existing problems in the prior art such as non-uniform diffusion of the conventional stirred reaction solution, resulting in particle agglomeration, and higher requirements for the type and conditions of the polymerization reactio...

Method used

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  • Molecularly-imprinted nanoparticle for glycoprotein and synthesis method thereof
  • Molecularly-imprinted nanoparticle for glycoprotein and synthesis method thereof
  • Molecularly-imprinted nanoparticle for glycoprotein and synthesis method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] In this example, a polytetrafluoroethylene capillary microfluidic reactor with an inner diameter of 800 μm and a length of 5 m was used. The functional monomer is a phenylboronic acid silylating reagent, and its preparation method is as follows: 3-formylphenylboronic acid (FPBA, 7.5 mg, 0.5mmol) is dissolved in 25 mL of ethanol, and then 3-aminopropyltriethoxy silane (APTES, 120 μL, 0.5 mmol), stirred at room temperature for 12 h, and finally removed the solvent ethanol by a rotary evaporator under vacuum to obtain phenylboronic acid silylating reagent FPBA-APTES, which can be used as Functional monomers are used to prepare glycoprotein molecularly imprinted polymers. The elution solution used was 100 mM acetic acid solution (containing 60% methanol, v / v) acetic acid solution.

[0032] Ovalbumin molecularly imprinted nanoparticles were prepared according to the following steps, and the synthesis process diagram can be found in figure 1 :

[0033] Step 1. Preparation...

Embodiment 2

[0038] Same as Example 1, the difference is that the inner diameter of the polytetrafluoroethylene capillary microfluidic reactor in this example is 500 μm.

[0039] The glycoprotein molecularly imprinted nanoparticles prepared in this example were named MIP-500. The yield of prepared MIP-500 was 17.8%.

Embodiment 3

[0041] Same as Example 1, the difference is that the inner diameter of the polytetrafluoroethylene capillary microfluidic reactor in this example is 250 μm.

[0042] The glycoprotein molecularly imprinted nanoparticles prepared in this example were named MIP-250. The yield of prepared MIP-250 was 13.6%.

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Abstract

The invention discloses a molecularly-imprinted nanoparticle for glycoprotein and a synthesis method thereof, belongs to the field of analytical chemistry, and relates to synthesis of molecularly-imprinted polymers. The synthesis method comprises the following steps: firstly, preparing a solution phase A from template glycoprotein molecules, preparing a solution phase B from a functional monomer and a cross-linking agent, and preparing solution phase C from an initiator; secondly, respectively fixing the solution phase A, the solution phase B and the solution phase C on three micro-fluidic injection pumps with adjustable flow rates, connecting and mixing the solution phase A and the solution phase B through a Y-shaped three-way valve, connecting and mixing the mixed solution and the solution phase C through another Y-shaped three-way valve, and enabling the obtained mixed solution and the solution phase C to flow into a reactor for a polymerization reaction; and finally, collecting a polymerized nanoparticle at the tail end of the reactor, and removing the glycoprotein template molecules to obtain the molecularly-imprinted nanoparticle for glycoprotein. The molecularly-imprinted nanoparticle for glycoprotein is prepared in a microfluidic mode, operation is easy, continuous production can be achieved, and the obtained molecularly-imprinted nanoparticle can be used for specific recognition of target glycoprotein molecules.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to the synthesis of molecularly imprinted nanoparticles, in particular to a glycoprotein molecularly imprinted nanoparticle and a synthesis method thereof. Background technique [0002] Molecularly Imprinted Polymer (MIP) is a high molecular polymer with special molecular recognition ability. The formation principle of its selective recognition ability is: firstly, the template molecule and the functional Covalently interact to form a complex, then add a cross-linking agent and an initiator to obtain a molecularly imprinted pre-polymerization solution; then, initiate a polymerization reaction to obtain a high molecular weight polymer containing template molecules; finally, remove the template molecules in the polymer, so that in An imprinted cavity complementary to the shape of the template molecule will be left in the polymer, and the functional group of the functional monomer re...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30C08G12/06G01N21/33
Inventor 李前进金雨王婷婷王奋英李建林
Owner NANJING NORMAL UNIVERSITY
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