880 results about "Cross-Linking Reagents" patented technology

The invention discloses immobilized bacteria-algae microspheres, a preparation method thereof and application of the immobilized bacteria-algae microspheres in removal of ammonia nitrogen and total phosphorus in aquaculture water. The preparation method comprises the following steps: adding polyvinyl alcohol and sodium alginate into deionized water, heating and sterilizing while fully dissolving polyvinyl alcohol and sodium alginate to obtain an embedding medium; adding the iron modified biochar powder into a mixed solution of chlorella and photosynthetic bacteria for adsorption to obtain a bacteria-algae-biochar mixture; adding the bacteria-algae-biochar mixture into an embedding medium to form a gel solution; adding the gel solution into a cross-linking agent for cross-linking, and after cross-linking, separating a solid and cleaning to obtain immobilized bacteria-algae microspheres; the preparation method of the iron-modified biochar powder comprises the following steps: adjusting the pH value of a mixed solution containing biochar powder, Fe < 2 + > and Fe < 3 + > to 9.5-10.5, carrying out heat preservation stirring at 75-85 DEG C for 1-5 hours, carrying out solid-liquid separation, taking a solid, washing, and drying to obtain the iron-modified biochar powder.

The invention relates to the technical field of biological adhesives, in particular to a pH response type chitosan medical biological adhesive and a preparation and application method thereof.The preparation method includes the steps that phenylboronic acid modified chitosan, polyphenol compounds, cross-linking agents with different functional groups and polyethylene glycol with different molecular weights are evenly mixed in a PBS buffer solution with the pH equal to 7-8; and adjusting the pH value of the system to 7.5-8 by using a KOH aqueous solution, and standing at room temperature until the pH-responsive chitosan medical biological adhesive is formed. The chitosan biological adhesive disclosed by the invention still keeps excellent adhesive power in a moist environment, is rapid in adhesion, is non-toxic and biodegradable in material, has good biocompatibility, and effectively avoids secondary stitch removal pain caused by non-degradable materials. Due to the charge characteristic of the chitosan, the chitosan has good affinity with human tissues, is suitable for adhesion of various tissues, and has wide clinical application potential.

The invention provides a preparation method of immobilized D-psicose-3-epimerase, which comprises the following steps: firstly aminating inorganic silicon nanoflowers, then activating by using a cross-linking agent glutaraldehyde, finally adding enzyme liquid, reacting in a shaking table for a period of time, and centrifuging and washing the reactant to obtain the product, namely the immobilized D-psicose-3-epimerase. The enzymatic activity and the stability of the immobilized enzyme are obviously improved, the specific enzyme activity of the immobilized enzyme is 446.44-1033.09 U / g protein, the activity of the immobilized enzyme is 163.09% and 181.53% of that of free enzyme after the immobilized enzyme is incubated for 6 h under the condition that the temperature is 30 DEG C or the pH value is 5, 63.18-70.78% of the initial activity is still reserved after the immobilized enzyme is repeatedly used for 10 times, and 46.27-54.38% of the initial activity is still reserved after the immobilized enzyme is stored for 15 days. The immobilized enzyme can catalyze fructose to generate psicose, and a green and efficient preparation technology is provided for production of psicose.

The invention relates to the technical field of hydrogel, in particular to a preparation method and application of EGCG-mediated hydrogel, a lysozyme solution is heated under the acidic condition to form lysozyme amyloid fibrils LAFs, EGCG is dissolved in a buffer solution and mixed with the LAFs solution obtained in the step 1 in proportion to form an LAFs-EGCG compound, and the EGCG-mediated hydrogel is prepared. The chitosan CS is dissolved in an acetic acid buffer solution, glutaraldehyde is added to serve as a cross-linking agent, the mixture is mixed with the LAFs-EGCG compound obtained in the step 2 and stirred, the composite hydrogel is formed, the concentration of EGCG is 0.2-1.0 wt%, the concentration of LAFs is 5 wt%, the concentration of chitosan is 2 wt%, the pH value of the lysozyme solution in the step 1 is 2.0, the heating temperature is 77 DEG C, the heating time is 10 hours, the concentration of EGCG in the step 2 is 0.4-0.8 wt%, and the pH value of the lysozyme solution in the step 2 is 1.5-2.5. In the step 3, the concentration of glutaraldehyde is 2%, the storage modulus G'of the hydrogel is 325-480 Pa, the hardness is 2176.71 g, and the bacteriostasis rate on staphylococcus aureus and salmonella exceeds 95%.

The invention belongs to the technical field of biomedical materials, and discloses injectable heterogeneous cascade nano-enzyme antibacterial hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: in a process of preparing cerium dioxide nano-enzyme by a reverse micelle method, introducing molybdenum disulfide nano-particles with a nano flower-shaped structure so as to deposit the cerium dioxide nano-enzyme on the surfaces of the molybdenum disulfide nano-particles in situ to obtain nano-composite particles; through electrostatic interaction, glucose oxidase is loaded on the surfaces of the nano composite particles to form a heterogeneous cascade nano enzyme material; the preparation method comprises the following steps: uniformly mixing sodium alginate and hyaluronic acid in water, and sequentially adding an NO donor, a heterogeneous cascade nano-enzyme material and a cross-linking agent to obtain the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel. The preparation method is simple, and the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel has a strong antibacterial effect and can be used for preparing the antibacterial hydrogel. Particularly, the compound has a remarkable killing effect on gram-negative bacteria and gram-positive bacteria.

The invention provides a preparation method of a recombinant collagen dressing, and belongs to the technical field of medical dressings. According to the technical scheme, stacked short sequences and continuous human collagen amino acid short sequences are repeatedly combined for multiple times to form recombinant collagen with specificity, and high similarity of the recombinant collagen with human collagen is ensured. On the basis, 4-FPBA is used as a cross-linking agent to respectively form a Schiff base bond responsive to pH and a boric acid ester bond responsive to glucose with CMCS and two kinds of HA with different molecular weights, and then the double-dynamic covalent cross-linked network adhesive hydrogel is synthesized. The triple-helix structure of the recombinant collagen is combined with a specific receptor on a cell membrane, and cell behaviors are regulated and controlled by inducing signal transduction through a cytoskeleton, so that skin microcirculation can be effectively improved, and a skin barrier can be repaired; the hydrogel dressing matrix has the effects of resisting inflammation, resisting oxidation, resisting bacteria, promoting healing and the like, the biological performance of the dressing is remarkably improved, and a better curative effect is achieved.

The invention provides a preparation method of starch hydrogel microspheres, and belongs to the technical field of embolism microsphere manufacturing. The preparation method of the starch hydrogel microspheres comprises the following steps: adding a first cross-linking agent into a starch solution, and mixing to obtain a water-phase solution; adding the oil phase solution into the water phase solution, and mixing to obtain a mixed liquid; adding a second cross-linking agent into the mixed liquid, mixing and reacting to obtain the starch hydrogel microspheres, and the starch hydrogel microspheres prepared by the method have the advantages of long in-vivo degradation time and adjustable degradation time, and can be suitable for more disease types.

The invention relates to the technical field of hydraulic fracturing exploitation of deep / ultra-deep oil and gas reservoirs, and discloses a gel-coated temporary plugging agent and a preparation method and application thereof.The gel-coated temporary plugging agent comprises a proppant inner core and a degradable gel shell coating the outer surface of the proppant inner core; the degradable gel shell is formed by a gel shell gelling solution, the gel shell gelling solution comprises small-molecule comonomers and a degradable cross-linking agent, and the small-molecule comonomers comprise acrylamide, N, N-dimethylformamide, N, N-dimethylformamide, N, N-dimethylformamide, N, N-dimethylformamide, N, N-dimethylformamide, N, N-dimethylformamide the initiator is one or more of N, N-dimethylacrylamide, acrylic acid, hydroxyethyl acrylate, benzyl acrylate, 2-propyl acrylic acid, 2-acrylamide-2-methylpropanesulfonic acid and N-vinyl pyrrolidone; and the initiator is one or more of N, N-dimethylacrylamide, acrylic acid, hydroxyethyl acrylate, benzyl acrylate, 2-propyl acrylic acid, 2-acrylamide-2-methylpropanesulfonic acid and N-vinyl pyrrolidone. The gel-coated temporary plugging agent can be used for effectively plugging in a fracturing time period at high temperature / ultrahigh temperature, and the flow conductivity of fractured cracks can be enhanced by exposing an internal propping agent after the gel-coated temporary plugging agent is degraded.

The invention discloses a kind of preparation method of amino hyperbranched siloxane and its modified polyimide aerogel material.First, alkoxysilane X with amino active functional group, alkoxysilane Y of inactive functional group and water are carried out autocatalytic controlled hydrolysis condensation under sub-stoichiometric ratio, obtain multifunctional group (such as other active groups, alkoxyl group, amino) amino hyperbranched siloxane liquid (HPSi NH2) with controllable amino content, then using HPSi NH2 as cross-linking agent and anhydride-terminated polyamic acid solution reaction to obtain functionalized polyamic acid, which is stirred with tertiary amine and deionized water to obtain functionalized polyamic acid wet gel, which is passed through freeze drying, high temperature imidization treatment to obtain amino hyperbranched siloxane modified polyimide aerogel material. This material has excellent flexibility, resilience, heat resistance and flame retardancy, and can be applied to aerospace, communication, catalysis and filtration fields.

A nanostructured low-immunogenic biological artificial blood vessel, a preparation method therefor and a use thereof. The preparation method comprises: decellularizing a pre-treated animal blood vessel to obtain a decellularized blood vessel; treating the decellularized blood vessel in an enzyme solution to obtain an enzyme-treated blood vessel, wherein the enzyme solution comprises nuclease and / or a biological enzyme; and using a cross-linking agent to cross-link the enzyme-treated blood vessel to obtain a nanostructured low-immunogenic biological artificial blood vessel, which is used as a blood vessel transplantation material. The artificial blood vessel overcomes the defects of decreased mechanical properties in decellularized biological tissues, in-vivo calcification after long-term use, and the presence of immunogenicity, antigenic components such as vascular wall cells and cell nuclei are fully removed, and original collagen and other extracellular matrix structural proteins in the tissue are retained to a greater extent, avoiding the in-vivo calcification of the blood vessel upon long-term implantation, enhancing the durability of use of the blood vessel; and the method has a short preparation cycle, low cost and high long-term patency rate.

The invention discloses an enzyme immobilization method based on charge directed crosslinking, and belongs to the technical field of enzyme immobilization. The method sequentially comprises the following steps: reacting an enzyme with a modifier containing an anionic group to generate an electronegative modified enzyme; dissolving a protonated amino carrier in an acidic buffer solution to form a first solution, and dispersing a dissociation anion group-containing carrier and a modification enzyme in an alkaline buffer solution to form a second solution; mixing the two solutions, and constructing a gel network immobilized enzyme through electrostatic crosslinking; and finally, strengthening the gel network by using a multivalent metal ion solution. Directional anchoring of the enzyme in the gel is achieved through charge matching, the network stability and the enzyme microenvironment are synchronously optimized in combination with metal ion coordination, use of a covalent cross-linking agent is avoided in the whole process, conformation damage of an enzyme active center is avoided, the activity retention rate, the operation half-life period and the substrate mass transfer efficiency of the immobilized enzyme are remarkably improved, and the immobilized enzyme has good application prospects. The method is suitable for efficient immobilization of industrial enzymes such as beta-glucosidase, and has high biocompatibility and industrial potential.

The invention discloses an oral monoclonal antibody micro-coated nanopolysaccharide microsphere preparation for targeted therapy of inflammatory bowel diseases and a preparation method thereof. According to the method, oxidized mannan and an anti-TNF-alpha monoclonal antibody are mixed and cross-linked by a cross-linking agent containing a diselenide bond to form the drug-loaded nanoparticles. Mixing the drug-loaded nanoparticles with pectin, dropwise adding the mixture into a calcium chloride solution through an electrostatic spinning device, and carrying out ionic crosslinking to obtain the oral monoclonal antibody micro-coated nano polysaccharide microsphere preparation with the particle size range of 180-200 microns. The drug-loaded polysaccharide microsphere delivers the wrapped drug-loaded nanoparticles to a colitis disease part through electrostatic interaction. The drug-loaded nanoparticles are targeted to macrophages, release of the monoclonal antibody is accelerated under the triggering of high-concentration active oxygen at an inflammation part, and the oral stability and the treatment effect of the monoclonal antibody drug are improved. The polysaccharide is used as a monoclonal antibody targeted delivery carrier, raw materials are cheap and easy to obtain, the preparation process is simple and convenient, reaction conditions are mild, and application and development are easy.

The invention discloses a recombinant III-type human collagen composition. The recombinant III-type human collagen composition comprises recombinant III-type human collagen, a first buffer solution and a stabilizer, the stabilizer is selected from at least one of glycerol, butanediol, pentanediol, mannitol and trehalose. According to the recombinant III type human collagen composition, a chemical cross-linking agent or an enzymatic promoter is not added for cross-linking, and the used reagents are free of cytotoxicity, sensitization and other safety risks. Therefore, the recombinant III-type human collagen composition has no crosslinking agent residue, can form a three-dimensional network structure, can avoid the problem that the recombinant III-type human collagen and the polysaccharide are compounded to generate precipitates, also has good biocompatibility, degradability and high stability, especially has relatively high thermal stability, and can be used for preparing the recombinant III-type human collagen composition. The wound healing and repairing can be promoted, the healthy skin ecology can be reconstructed, and a new choice is provided for a biomedical material.

The invention discloses a preparation method of a polyphenol-containing medical adhesive. The preparation method comprises the following steps: respectively preparing a gelatin solution, a gallic acid solution and a tea polyphenol solution; mixing the gelatin solution and the gallic acid solution, and reacting at 40-60 DEG C for 6-12 hours to obtain a modified gelatin solution; mixing the tea polyphenol solution with the modified gelatin solution, adding an alkaline solution to adjust the pH value to 6.5-8.0, and uniformly mixing to obtain a mixed solution; the mixed solution is aged at 20-40 DEG C, water generated in the aging process is removed, and the polyphenol-containing medical adhesive is obtained. According to the preparation method of the polyphenol-containing medical adhesive, a gelatin protein molecular chain is used as a framework material, gallic acid is introduced into the protein molecular chain, tea polyphenol is used as a main chain cross-linking agent, the polyphenol-containing medical adhesive is prepared, a phenolic hydroxyl group can penetrate through a water boundary layer on the surface of a base material to form an adhesive bond with the base material, and the medical adhesive has the advantages that the water boundary layer can penetrate through the water boundary layer; good wet adhesion is shown on tissues.

The invention relates to the field of hydrogel preparation, and discloses anti-scale zwitter-ion hydrogel as well as a preparation method and application thereof. The method comprises the following steps: firstly, initiating an oxazine derivative monomer to polymerize through a double-end initiator to generate dihydroxyl polyoxazine; and then, removing a side chain of the polymer to obtain the dihydroxy polypropylene imine. Then, dihydroxy polypropylene imine is selected for further reaction to generate a zwitter-ion precursor polymer, and the zwitter-ion precursor polymer is hydrolyzed to obtain the polypropylene imine zwitter-ion derivative. And finally, converting hydroxyl groups at two ends, synthesizing a zwitterionic macromolecular cross-linking agent, and carrying out polymerization cross-linking in an aqueous solution to prepare the anti-scale zwitterionic hydrogel. The hydrogel is simple in formula, is completely composed of the zwitterionic cross-linking agent, has excellent anti-scaling performance such as protein adsorption resistance, bacterial adsorption resistance and cell adsorption resistance, and is suitable for biological materials, medical devices, implant materials and cell delivery materials.

The invention discloses a cationized silk fibroin / oxidized sodium hyaluronate injectable hydrogel as well as a preparation method and application thereof.The cationized silk fibroin / oxidized sodium hyaluronate injectable hydrogel is prepared by taking silk fibroin (SF) and sodium hyaluronate (SH) as main raw materials, performing cationization modification on the SF to obtain CSF, performing oxidation treatment on the SH to obtain OSH, and then adding the CSF and the OSH into the injectable hydrogel. The CSF / OSH injectable hydrogel with good adhesion, mechanical property, self-healing property, biocompatibility and antibacterial property can be formed through crosslinking by utilizing a Schiff base reaction between an amino group contained in the CSF and an aldehyde group contained in the OSH without adding any crosslinking agent. The CSF / OSH injectable hydrogel provided by the invention can play an important role in the treatment fields of wound healing, bone defects, cardiovascular diseases, brain diseases and the like, and has a wide application prospect in the fields of biomedicine and tissue engineering.

The invention relates to the technical field of adhesives, and provides a wet state maintaining type vegetable protein adhesive as well as a preparation method and application thereof. The hyperbranched catechol polymer adhesive filler disclosed by the invention is prepared by carrying out ring-opening reaction on epoxy groups of neopentyl glycol diglycidyl ether and pentaerythritol tetraglycidyl ether and amino groups of dopamine hydrochloride. Meanwhile, the vegetable protein adhesive is prepared from vegetable protein, a cross-linking agent and the adhesive filler according to a certain proportion. According to the adhesive filler and the corresponding vegetable protein adhesive, the defect that in the prior art, a bio-based adhesive, especially a vegetable protein adhesive, is poor in product performance due to poor moisture retention can be overcome.

The present invention belongs to the technical field of material preparation, and specifically relates to the preparation and application of a hydroxyapatite microsphere composite injection. The present invention first provides a method for preparing hydroxyapatite composite microspheres, wherein the hydroxyapatite composite microspheres are modified hydroxyapatite microspheres with amino groups and carboxylic acid groups, and the method comprises preparing hydroxyapatite microspheres, preparing modified hydroxyapatite microspheres with amino groups, and preparing modified hydroxyapatite microspheres with amino groups and carboxylic acid groups. Without introducing a toxic crosslinking agent, the electrostatic adsorption between the groups is utilized to achieve uniform dispersion of the hydroxyapatite microspheres in an aqueous solution.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention discloses a preparation method and application of a marine high-molecular polysaccharide hydrogel wound dressing. The hydrogel is formed by cross-linking the marine polysaccharide composition and the cross-linking agent, and the gelation time is short and is about 15 seconds; pores are uniform and moderate; the self-healing property, the adhesion property and the swelling property are high; the antibacterial effect is strong, and the inhibition rates on escherichia coli and staphylococcus aureus are both higher than 90%; skin irritation and skin allergy are avoided, and the biological safety is high; hemostasis can be rapidly carried out within 10s, and the bleeding amount is obviously reduced; the hydrogel dressing can rapidly shrink and scab a wound surface, has the effect of promoting wound healing, and has the effects of stopping bleeding and promoting wound healing which are obviously superior to those of commercially available hydrogel dressings (German Hydrosorb Gel) (Plt; 0.05), and is a safe and effective high-performance wound dressing. In addition, the hydrogel is simple in formula and high in industrialization prospect, is expected to be developed into novel wound dressings and medical instruments, and breaks through the trapping that domestic hydrogel dressings are mostly dependent on import.

The present invention belongs to the technical field of waterborne polyurethane, and specifically relates to a method for synthesizing an internally cross-linked cationic waterborne polyurethane emulsion. The method comprises the following steps: 1) reacting glycidol and diethanolamine until the infrared spectrum analysis shows that the glycidol and diethanolamine are at 908 cm ‑1 and 845cm ‑1 The epoxy groups disappear, the reaction is stopped, and the product EDEOA, a tertiary amino crosslinking agent, is obtained as a light yellow viscous liquid; 2) polyol and diisocyanate are added to a reactor and heated and kept warm under stirring for reaction; 3) solvent, tertiary amino crosslinking agent, tertiary amino small molecule alcohol amine chain extender, solvent and catalyst are then added to react to obtain a polyurethane intermediate; 4) a neutralizing agent is then added to the reaction system for neutralization reaction, and the temperature is lowered after the reaction is completed; 5) water is added to quickly disperse the mixture uniformly, and the solvent is removed in vacuo to obtain a waterborne polyurethane emulsion. The present invention mainly solves the technical problems existing in the synthesis of cationic waterborne polyurethanes, such as poor emulsion stability, low film mechanical properties, and poor water and solvent resistance.

The invention relates to the technical field of cleaning agents, and discloses a biodegradable environment-friendly cleaning agent, which comprises: a composite surfactant system comprising at least one nonionic surfactant, an ampholytic surfactant and a chelating functional surfactant; the plant extract oil system comprises limonene and a eucalyptus oil mixture; the functional particle system comprises nanoscale enzyme microcapsules and slow-release type oxidized particles; the natural polysaccharide stable network system comprises at least two polysaccharide thickeners and a bio-based cross-linking agent, and the assistant system comprises a metal ion chelating agent, a buffer acid system and water. By introducing sodium polyaspartate as a green chelating surfactant, efficient chelating of calcium and magnesium ions under a hard water condition is realized, the cleaning power is remarkably improved, water stain residues are reduced, and the cleaning performance with wider adaptability is obtained.

The invention relates to the technical field of fertilizers and pesticides for agricultural production, and discloses a liquid pesticide fertilizer for preventing and treating root-knot nematodes in a water-fertilizer integrated system and application thereof, and the liquid pesticide fertilizer is prepared from the following components: a plant source nematicide, a biocontrol inoculant, a macroelement and medium trace element fertilizer, a boron cross-linking agent, a modified polycarboxylic acid dispersing agent, a microbial protective agent and the like. The preparation method comprises the steps of biological phase pretreatment, matrix phase construction, oil phase micro-emulsification, multiphase fusion and dynamic crosslinking. Through trehalose gradient permeation pretreatment, the survival rate of biocontrol bacteria in a high-salt matrix is increased; a boric acid ester bond dynamic cross-linked network is constructed by using a variable pH adjusting process, so that the system is endowed with shear thinning characteristics, and the contradiction between suspension stability and drip irrigation fluidity is solved; and the chelating dispersion effect of the modified polycarboxylic acid dispersing agent is matched, so that the generation of precipitates is effectively inhibited, and stable coexistence and synergistic interaction of the pesticide, the fertilizer and the bacteria are realized.

The invention discloses a capsule medicament for endoscopic treatment of ulcerative colitis and a preparation method thereof, and belongs to the technical field of biological medicine, the capsule medicament is composed of a capsule matrix and a drug matrix; by adopting a dual control mechanism of an enteric layer coating and a pH response layer coating, accurate release of a medicine at a colon diseased region is ensured, medicine waste of the stomach and the small intestine is reduced, the medicine concentration at a focus is improved, mesalazine or budesonide is combined with the composite composition for treatment, inflammatory response is rapidly inhibited, acute symptoms are controlled, and the curative effect is good. A multi-mechanism synergistic treatment mode is adopted, the anti-inflammatory and repairing effects are enhanced, the clinical remission rate and the mucous membrane healing rate are remarkably improved, the pH response layer is dissolved in a colon alkaline environment, and the MMP-9 peptide cross-linking agent is subjected to enzymolysis at an inflammation part, so that the medicine is continuously released at a focus, the administration frequency is reduced, the compliance of a patient is improved, and the curative effect is good. In addition, the spherical gel particles in the medicament can prevent the medicament from being disintegrated too early in the intestinal tract, and the long-acting effect can be maintained.

The invention discloses a tough and mildew-proof formaldehyde-free soybean protein adhesive as well as a preparation method and application thereof, defatted soybean powder is taken as a main agent, an epoxy compound is taken as a cross-linking agent, polyethyleneimine modified chitosan is used for synthesizing a modifier to prepare the modified soybean protein adhesive, and a compact cross-linked network structure is constructed, so that the toughness and mildew-proof formaldehyde-free soybean protein adhesive is obtained. According to the soybean protein-based adhesive, the performance of the soybean protein-based adhesive is successfully improved, the soybean protein-based adhesive is high in bonding strength, relatively good in water resistance and good in mildew resistance, the production process is simple and convenient, the production cost is greatly reduced, chitosan and polyethyleneimine are subjected to graft modification, the living environment of mildew is destroyed, and the mildew resistance of the soybean protein-based adhesive is improved. The epoxy compound and the chitosan-polyethyleneimine are compounded and cooperated, so that a stable net-shaped cross-linked structure is formed in the adhesive, and the water resistance and the mildew-proof capability of the adhesive are greatly improved.

A treatment composition that includes a treatment adjunct and a population of core / shell delivery particles, where the shell is made from an acid-treated chitosan and a cross-linking agent, where the acid-treated chitosan results from treating chitosan with a weak acid, or even a mixture of a strong acid and a weak acid. Related methods of making and using such compositions.

The invention belongs to the technical field of gel dressings, and particularly relates to a bio-based gel dressing for wound treatment and a preparation method thereof.The bio-based gel dressing is prepared from, by weight, 10-17 parts of mussel mucin, 13-20 parts of silk fibroin, 1-5 parts of plant vesicles, 2-4 parts of traditional Chinese medicine polysaccharide, 0.05 part of antibacterial peptide, 1.5 parts of glycerinum and 0.3 part of cross-linking agent; nano plant vesicles which are extracted from a traditional Chinese medicine prescription and have the effect of promoting wound healing are used as a carrier for encapsulating antibacterial peptide, and then the antibacterial peptide is encapsulated in a three-dimensional gel network formed by natural protein, so that controlled release and slow release of antibacterial and anti-inflammatory components are realized, and efficient wound healing is promoted.

The invention discloses a preparation method of PBA nanoparticle in-situ modified halloysite nanotube-polyethyleneimine cross-linked composite aerogel, which comprises the following steps: modifying halloysite nanotubes by using an epoxy silane coupling agent to obtain epoxidized HNTs, and further grafting ethylenediamine on the surfaces of the epoxidized HNTs to obtain HNTs rich in amino groups and hydroxyl groups; the preparation method comprises the following steps: respectively stirring and dissolving K4Fe (CN) 6.3 H2O and polyvinylpyrrolidone in a hydrogen chloride solution, then adding HNTs rich in amino groups and hydroxyl groups, ultrasonically dispersing uniformly, and reacting to obtain PBA-coated HNTs; the preparation method comprises the following steps: taking polyethyleneimine as a raw material, taking 1, 3-dichloropropanol as a cross-linking agent, adding an acid-binding agent, carrying out a cross-linking reaction on the polyethyleneimine and PBAatHNTs, finally freeze-drying a reaction solution, then removing the residual acid-binding agent and salts thereof by soaking and cleaning, and drying again to obtain a finished product of aerogel. According to the invention, the problems that polyethyleneimine is easy to remain in a solution and a nano-powder material is not easy to recover and reuse are solved, and the adsorption performance on heavy metal ions is improved.

The invention is applicable to the technical field of biomedicine, and provides dipeptide-photosensitizer molecule co-assembled nanoparticles as well as a preparation method and application thereof. According to the invention, cationic diphenylalanine (CDP) and a biological cross-linking agent genipin are self-assembled to form a dipeptide carrier, and then the dipeptide carrier and a photosensitizer molecule chlorin e6 (Ce6) are co-assembled, so that nanoparticles with controllable size, controllable drug loading capacity and good biocompatibility are successfully prepared. The preparation method is simple and easy to implement, effectively solves the key problems that the traditional photosensitizer molecule Ce6 is poor in biocompatibility and easy to gather, not only can efficiently deliver Ce6, but also obviously improves the application safety and biocompatibility of Ce6. The Ce6 is almost free of dark toxicity under a dark condition, can play a strong photodynamic killing role under illumination, and greatly improves the possibility and application potential of the Ce6 in tumor photodynamic therapy by virtue of the flexible regulation and control advantages of the size and the drug loading capacity.

The invention discloses potato starch nano-carrier immobilized lipase and a preparation method thereof, and relates to the technical field of biological catalysis. The immobilized lipase comprises a lipase and a carrier, and the lipase is immobilized on the carrier; the carrier is potato starch modified magnetic nanoparticles. The immobilized lipase provided by the invention takes the magnetic potato starch nanoparticles as a carrier and has the advantages of being green and environment-friendly and easily available in materials, and the lipase is successfully immobilized on the magnetic nanoparticles by using the potato starch and the traditional glutaraldehyde as cross-linking agents. The method not only improves the activity and stability of the immobilized enzyme, but also has good biocompatibility and safety. By optimizing parameters such as potato starch dosage, enzyme concentration and immobilization time, the obtained immobilized enzyme shows excellent storage stability and repeated utilization rate. In addition, the method provides reference for preparation of other high-performance immobilized enzymes.