1063 results about "Cross-Linking Reagents" patented technology

One aspect of the present invention generally relates to methods of sealing a wound or tissue plane or filling a void splace. In a preferred embodiment, the wound is an ophthalmic, pleural or dural wound. In certain instances, the compositions used to seal the wound or tissue plane comprises a polyalkyleneamine. In a preferred embodiment, the polyalkyleneamine is polyethyleneimine. Treatment of the polyethyleneimine with a cross-linking reagent causes the polyethyleneimine polymers to polymerize forming a seal. In certain instances, the cross-linking reagent is a polyethylene glycol having reactive terminal groups. In certain instances, the reactive terminal groups are activated esters, such as N-hydroxy succinimide ester. In certain instances, the reactive terminal groups are isocyanates. In certain instances, the polyethyleneimine has a lysine, cysteine, isocysteine or other nucleophilic group attached to the periphery of the polymer. In certain instances, the polyethyleneimine is mixed with a second polymer, such as a polyethylene glycol containing nucleophilic groups. In certain instances, the compositions used to seal the wound or tissue plane are formed by reacting a polyalkyleneamine bearing electrophilic groups with a cross-linking reagent containing nucleophilic groups. In certain instances, the electrophilic groups on the polyalkyleneamine are activated esters, such as N-hydroxy succinimide ester. In certain instances, the compositions used to seal the wound or tissue plane are formed by reacting a polyalkyleneamine bearing photopolymerizable groups with ultraviolet or visibile light. Compositions used to seal the wound which contain PEI or a derivative of PEI are found to adhere tightly to the tissue. Other aspects of the present invention relate to methods of filling a void of a patient or adhering tissue. In certain instances, the methods use a polyalkyleneamine. In a preferred embodiment, the polyalkyleneamine is polyethyleneimine. Another aspect of the present invention relates to a polymeric composition formed by exposing a polyalkyleneamine to an activated polyalkylene glycol. In certain instances, the composition is attached to mammalian tissue.

Methods for preparing cross-linked polysaccharide matrices by cross-linking one or more amino group containing polysaccharides or amino-functionalized polysaccharides with reducing sugars and/or reducing sugar derivatives. The resulting matrices may include polysaccharide matrices and composite cross-linked matrices including polysaccharides cross-linked with proteins and/or polypeptides. Additives and/or cells may also be included in or embedded within the matrices. Various different solvent systems and reducing sugar cross-linkers for performing the cross-linking are described. The resulting matrices exhibit various different physical, chemical and biological properties.

The invention discloses a biomacromolecule interpenetrating polymer network hydrogel and a preparation method of the biomacromolecule interpenetrating polymer network hydrogel. The biomacromolecule interpenetrating polymer network hydrogel is formed by crosslinking two kinds of enzymes in a catalysis mode, one is a polysaccharide macromolecule network formed by crosslinking polysaccharide macromolecules with introduced phenolic hydroxyl groups in an oxidation mode as oxidase and hydrogen peroxide catalyze phenolic hydroxyl groups, and the other is a protein or polypeptide macromolecule network formed crosslinking protein or polypeptide containing amino acid residues as transferase catalyzes peptide bonds. The two networks interpenetrate each other and form the novel interpenetrating polymer network hydrogel without bonding of chemical bonds. No chemical crosslinking agent is used in the hydrogel, and the hydrogel has excellent biocompatibility and mechanical property, can be shaped like a dry or wet film, like porous sponge or fibers, and can serve as a contact lens, a medicine release carrier, a scaffold for tissue engineering or materials for tissue repair.

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell/tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

The shape memory polymers disclosed are a reaction product of at least one reagent containing two active amino-hydrogen or two active phenolic-hydrogen with at least one multifunctional cross linking reagent which contains at least three or more active amino- or phenolic-hydrogen or is a reagent containing at least three glycidyl ether moieties which is then further mixed with at least one diglycidyl ether reagent whereupon the resulting mixture is cured and has a glass transition temperature higher than 00C. This reaction creates crosslinking between the monomers and polymers such that during polymerization they form a crosslinked thermoset network.

A gel depilatory composition obtainable by mixing (a) a keratin reducing compound, (b) a hydrophilic high molecular compound having an ionic group, (c) a crosslinking agent and (d) water and has a high effect on the removal of body hairs and is decreased in odor peculiar to chemicals.

The invention provides novel food compositions having a realistic meat-like appearance, feel, and texture. The compositions comprise from about 40 to about 90% functional proteins, from about 0.05 to about ²% of one or more cross-linking agents, and from about 60 to about 10% of a meat slurry, wherein the meat slurry comprises meat and one or more humectant plasticizers in a meat:humectant plasticizer ratio of from about 20:80 to about 80:20. The compositions are produced by heating a preconditioned mixture of the food components under pressure and then expanding the heated composition to form the food composition.

The present invention provides both crosslinked polymer compositions capable of forming hydrogels upon exposure to an aqueous environment and thiosulfonate hydrogel-forming components. The thiosulfonate hydrogel-forming components of the invention are preferably multi-arm thiosulfonate polymer derivatives that form a crosslinked polymer composition when exposed to a base without requiring the presence of a second cross-linking reagent, redox catalyst, or radiation. Methods for forming hydrogel compositions, as well as methods for using the hydrogels, are also provided.

Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semicylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method has patches of capture molecules, each specific for a different analyte disposed adjacently within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers, which in turn are coupled to the waveguide surface or to a nonspecific binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers, the selective irradiation involving a mask, a laser light source, or the like.

The invention relates to a novel composite material, and particularly relates to a preparation method and application of a nitrogen-doped graphene and cobaltosic oxide hollow nanosphere composite material. The novel composite material comprises a doped graphene substrate and cobaltosic oxide hollow nanospheres which is attached to the surface of the doped graphene substrate. Melamine resin is taken as a cross-linking reagent for integrating graphite oxide with Co<2+> into a single coordination precursor. The preparation method comprises the following steps: in a pyrolysis process of the precursor, taking the melamine resin as a new nitrogen source to uniformly dope the graphene with nitrogen, fixing cobalt oxide which is generated in situ, and finally preparing the nitrogen-doped graphene/Co3O4 hollow nanosphere composite material with a sandwich structure. The composite material has a graded porous structure, is high in specific surface area, more in active sties, good in electron conductivity and ion conductivity, and good in application prospect in the field of new energy resources and catalysis.

Disclosed are methods and systems for determining the three-dimensional structure of chromatin in eukaryotic cells. More specifically, disclosed are methods and systems for obtaining chromatin structural information by surface immobilization, i.e tethering crosslinked protein:DNA complexes and/or ligated DNA complexes to media such as beads, gels, and or matrices during the conformation capture assay. In general, the method includes contacting a cell with a cross-linking reagent to cross-link DNA and protein in the cell; lysing the cell, producing cross-linked protein:DNA complexes by cutting the chromatin using a chemical, physical or enzymatic method, substantially immobilizing the cross-linked protein:DNA complexes, ligating the cross-linked protein:DNA complexes intramolecularly such that the ligated protein:DNA complexes represent structural organization of the chromatin; characterizing the ligated DNA by sequencing or other methods; and identifying any structural organization of the chromatin. The structural organization preferably includes information relating to interacting loci of the chromatin.

The invention discloses an application of injectable hydrogel in preparing intraocular filling materials. The application is characterized by being application of the injectable hydrogel as intraocular filling materials in vitreoretinal surgery as well as application of the injectable hydrogel as an intraocular drug carrier, wherein the injectable hydrogel is composed of two agents; the first agent is a glue solution containing oxidized polysaccharide; the second agent is a glue solution containing a chitin derivative and /or collagen; the two agents are separately contained in two injection tubes of a duplicate injector; the two glue solutions are simultaneously pushed and injected by the duplicate injector; in the pushing and injecting process, mixing and cross-linking are generated, namely, a dialdehyde group of the oxidized polysaccharide generates cross-linking reaction with amino of the chitin derivative and/or the collagen to form hydrogel with viscoelasticity. The constituents of the hydrogel are biomacromolecules different from chemical macromolecules, and a micromolecule cross-linking agent is not introduced, so that the biocompatibility is good, the hydrogel can be degraded and absorbed, toxic and side effects are avoided, and the safety is good.

The invention discloses a multifunctional composite adsorbent. Cross-linked chitosan is used as a matrix and is formed by cross-linking heavy metal ion imprinting chitosan by using a cross-linking agent; the cross-linked chitosan matrix is filled with nano Fe3O4 and nano TiO2; and the surface of the multifunctional composite adsorbent is modified by thiocarbamide so as to form a large amount of free amino groups. The invention correspondingly discloses a preparation method for the multifunctional composite adsorbent. The preparation method comprises the following steps of: performing photocatalysis loading, performing heavy metal ion imprinting to ensure the metal imprinting ions are fully reacted with chitosan in the heavy metal imprinting ions to form complexes, and adding the cross-linking agent and reacting; and adding epichlorohydrin and an acetone solution containing thiocarbamide, and modifying and grafting, finally oscillating and desorbing, and regenerating by using an alkaline solution to prepare the multifunctional composite adsorbent. By using the multifunctional composite adsorbent, heavy metal can be selectively separated and/or organic pollutants can be degraded.

An embodiment of the invention provided herein is a pharmaceutical composition comprising a gastrin compound having an extended activity upon administration to a subject in comparison with native gastrin. Methods are provided of conjugating portions of the amino acid sequence of gastrin having functional ability to bind to the gastrin/CCK receptor, to various carrier moieties, including the use of amino acid spacer regions, and use of bifunctional cross-linking reagents. Methods of treating a diabetes patient with the compositions are provided.

The invention discloses a preparation method of a lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe. The preparation method adopts quantum dots as carriers and lysozyme as a template molecule and modifies a molecular imprinting polymer layer on surfaces of the quantum dots to synthesize the novel lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe through combining selectivity of a molecular imprinting technology and fluorescent characteristics of the quantum dots. Specially, the preparation method comprises the following steps of preparing quantum dots, modifying surfaces of the quantum dots by denatured bovine serum albumin, forming a molecular imprinting layer through a polymerization reaction initiated by a selected functional monomer and cross-linking reagents, and washing away the template molecule by selected eluent. The lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe can combine accuracy and specific identification characteristics of molecular imprinting and high sensitivity characteristics of quantum dot fluorescence detection and has high selectivity and sensitivity of template molecule identification. The preparation method has simple processes, good reproducibility between different batches, good application prospects in selective identification and detection of lysozyme in an actual sample.

The invention discloses a preparation method of an injectable hyaluronic acid hydrogel. The method comprises the following steps: initiating a condensation reaction of an amino group of adipic dihydrazide and a carboxyl group of the main chain of HA (hyaluronic acid) by 1-ethyl-3-(3-dimethylamino)carbodiimide hydrochloride to obtain a hyaluronic acid-adipic dihydrazide (HA-ADH) derivative with the main chain possessing the amino group, and reacting N-acryloxysuccinimide with the amino group of the HA-ADH to obtain an acrylate functionalized hyaluronic acid derivative; and cross-linking the acrylate functionalized hyaluronic acid derivative with a bismercapto cross-linking agent to form the injectable hyaluronic acid hydrogel. The above acrylate functionalized HA-AC synthesis method is simple and easy, and is suitable for industrial amplified production; the environmental protection, biological inertness and reaction speed controllability of a Michael addition reaction lay a good foundation for the application of the Michael addition reaction in the injectable hyaluronic acid hydrogel; and the HA-AC chemically cross-linked hydrogel has better stability and mechanical performances than physically associated injectable gels.

The invention relates to an analytical method for researching protein structure or protein-protein interaction. The method comprises the following steps: performing crosslinking and enzymolysis on a protein composite in a cell by using a crosslinking agent with reactive groups on two sides and a breakable group, and taking a part of the enzymatic hydrolysate for a derivatization reaction for mass spectrometry; after breaking the crosslinking agent by means of a chemical method for the other part of the enzymatic hydrolysate, enriching peptide sections with an enriching material, and performing mass spectrometry on the enzymatic hydrolysate without the enriched peptide sections; determining the crosslinked peptide sections according to a library searching result so as to establish a peptide section library; finding out a candidate peptide section from the peptide section library according to N-terminal amino acid information of the crosslinked peptide section determined in the mass spectrogram of the crosslinked peptide section; and determining the crosslinked peptide section sequence by combining the mass spectrogram m/z of the crosslinked peptide section and the characteristic ions of the peptide section so as to obtain the protein structure and protein-protein interaction information. The method has the advantage of being simple to operate, and is applied to structural analysis of proteins and analysis of protein-protein composite interaction.

The protein fiber spinning solution is produced by using animal and plant material and through the processes of acid pickling, extraction, pH regulation to obtain protein curd, dissolving with cosolvent to form water solution, mixing, modifying side chain of amino acid with modifying agent, copolymerization with coloring monomer, mixing with PVA water solution and addition crosslinking agent to regualte viscosity. It may be used in spinning fiber with the same strengt has chemical fiber, the skin friendship, air penetrability and hygroscopicity the same as natural protein fiber, and improved hot water resistance, shrinkage and color.

The invention discloses a decomposable crosslinking polyethylene imine and making method and application under physiological condition, which is characterized by the following: adopting one or more composition of glycidyl ester of each kind diacid, acroleic acid/methacrylic acid glycidyl ester or polyol or polyatomic condensed alcohol/polybasic ester of methyl acroleic acid as crosslinking agent; crosslinking with linear or branched polyethylene imine to form non-virus gene infection-transmitting carrier for each cell and biological tissue.

The invention discloses a long acting slow-release drug carrier material for therapy and repair of bone disease and preparation method; wherein, the components of the drug carrier material are 4-8 weight account of strontium-doped calcium polyphosphate, 1-3 weight account of chitosan drug-loaded microspheres and 1-3 weight account of chitosan; the preparation method of the compound drug carrier material for therapy of osteomyelitis and other bone diseases is that (1) the chitosan-acetic acid solution is prepared, (2) the strontium-doped calcium polyphosphate powder is put into the chitosan-acetic acid solution and dispersed evenly, (3) the prepared chitosan drug-loaded microspheres is added, (4) the cross-linking agent is added for cross-linking, (5) freezing at temperature of 2-6 DEG C is processed for 12-48 hours, and (6) heating and drying are processed at temperature of 40-100 DEG C. The drug carrier material has the advantages of being applicable to therapy and repair of osteomyelitis and other bone diseases, releasing antibiotic for a long period, bone repair function, good biocompatibility and degradability, and ideal double function of therapy and repair of osteomyelitis and other bone diseases.

The invention discloses an injectable type collagen-based soft tissue filling material and preparation method thereof, wherein the filling material mainly comprises collagen 0.5-20%, heparin 0.1%-10%, cell growth factor 0-1%, cushion liquid 70-99.4%, and its preparation comprises extracting soluble collagen from animal connective tissue, cutting off antigen deciding family from the collagen molecules through proteinase hydrolysis, purifying treatment, crosslinking by utilizing safety and nontoxic crosslinking agent, charging biologically active components, kneading the mixture repeatedly into grease form, sealing under asepsis condition and storing at low temperature.