545 results about "Enzyme agent" patented technology

The invention relates to an intelligent regulation and control method and system for fermentation parameters, a terminal and a medium, and belongs to the technical field of enzyme preparation fermentation. The intelligent regulation and control method for the fermentation parameters comprises the following steps: determining a current fermentation stage of a fermentation process; determining a dissolved oxygen target value and a pH target value according to the fermentation stage; acquiring monitoring data, wherein the monitoring data comprises a dissolved oxygen actual value, a dissolved oxygen change rate, a pH actual value, a pH change rate, a thallus concentration and a fermentation volume; if the change rate of the dissolved oxygen is a negative value, predicting a pH predicted value in future n minutes according to the actual value of the dissolved oxygen and the thallus concentration; judging whether an error value between the pH predicted value and the pH target value is not greater than an error threshold value or not; if yes, parameter intervention is carried out, and parameters comprise the ventilation capacity, the stirring speed and the alkali supplementing amount. The method has the beneficial effects that the fermentation parameters can be precisely regulated and controlled, so that the quality of a fermentation product is precisely improved.

A method for preparing a high-purity yeast β-glucan, comprising the following steps: treating a yeast cell wall mixed liquid by adding amylase at a certain temperature, then adjusting the pH, and treating the mixed liquid by adding lipase at a certain temperature; adding an alkali and a nonionic surfactant, carrying out heating and thermal insulation treatment, and cooling to obtain an alkali-treated liquid; washing and separating the alkali-treated liquid, adding water to a precipitate, then adding an acid to adjust the pH to 3-5, carrying out heating and thermal insulation treatment to obtain an acid-treated liquid, and performing separation, washing, and drying on the acid-treated liquid to obtain a yeast β-glucan product; and subjecting the yeast β-glucan to a combined action of a mixed enzyme preparation and ultrasound to obtain an enzymatic hydrolysate, and performing separation treatment on the enzymatic hydrolysate to obtain an insoluble yeast β-glucan and a water-soluble yeast β-glucan, respectively. In the present invention, a high-purity yeast β-glucan product having a content of 90% or more and a water-soluble yeast β-glucan product with biological activity can be prepared, the preparation method is simple, large-scale production can be achieved, and the quality and additional value of the product can be improved.

The invention relates to the technical field of biological agents, and discloses a passivating enzyme soluble concentrate and a preparation process thereof.The soluble concentrate takes a specially-designed multifunctional deep eutectic solvent as a unique carrier, and the solvent has the multiple functions of stabilizing enzyme conformation, resisting oxidation, providing plant metabolism and increasing energy and the like. An active component is composite passivating enzyme powder which is subjected to vacuum freeze drying, and is compounded with a plant-derived phloem conduction enhancer in a synergetic manner. The preparation process adopts a core strategy of curing first and then dissolving, and the enzyme freeze-dried powder is dispersed in the non-aqueous solvent under low-temperature mild conditions. By constructing a water-free environment and adopting an activity protection process, the problems that a traditional water-based enzyme preparation is unstable in storage and the activity is easy to lose are fundamentally solved, and meanwhile, the bioavailability is remarkably improved by virtue of a targeted conduction component. The preparation has the remarkable advantages of ultra-long stability at normal temperature, high initial enzyme activity and strong targeting property.

The utility model discloses a stirring device for industrial enzyme preparation production, which relates to the technical field of enzyme preparation production and comprises a tank body, outer channel steel for reinforcement is sleeved at the bottom of the tank body, a cover plate is hermetically connected to the top of the tank body, and a feed pipe penetrates through the cover plate. A discharging pipe communicated with the interior of the tank body is arranged on one side of the tank body, and an observation window is formed in the side wall of the tank body. The conveying rod is driven by the output shaft of the motor to rotate in the conveying pipe, fermentation liquor at the bottom of the tank body can be conveyed to the upper portion along the conveying pipe, damage of shear force to zymoprotein molecules is reduced, the fermentation liquor flows all around under the guidance of the guide plate and the arc-shaped groove, the circulation speed of the fermentation liquor in the tank body is increased, and the fermentation efficiency is improved. The cells are in full contact with nutrient substances, it can be ensured that temperature distribution in fermentation liquor is more uniform, local overheating or supercooling is avoided, and the growth speed and enzyme production efficiency of the cells are improved.

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

A laundry composition and a preparation method therefor are provided, relating to the field of household necessities. The laundry composition includes a wrapping material, a functional material enveloped in the wrapping material, and an adhesive for bonding the wrapping material; the wrapping material is a film-forming material with laundry function; the functional material is selected from one or more of a softening particle, a fragrant particle, an active oxygen particle, and an enzyme preparation. By enveloping the functional material in the wrapping material with a detergent function, the laundry composition has integrated functions of cleaning, decontamination, pliancy, and fragrance-retaining. Furthermore, since the granular softening particle, a granular fragrant particle, a granular active oxygen particle, and a granular enzyme preparation are not directly added in the wrapping material, even if the wrapping material is formed by high-temperature drying, the functional materials will not fail.

SOLUTION: A laundry composition comprises an encapsulation material, a functional component encapsulated within the encapsulation material, and a binder used for bonding the encapsulation material, wherein the encapsulation material is a film-forming material having a washing function, and the functional component comprises one or more of softening particles, fragrance-retaining particles, active oxygen particles, and an enzyme preparation.EFFECT: By encapsulating the functional component with the encapsulation material having the washing function, the laundry composition can simultaneously achieve a cleaning and stain removal function, a softening function, and a fragrance-retaining function. In addition, since the softening particles, the fragrance-retaining particles, the active oxygen particles, and the enzyme preparation in the functional component are not directly added to the encapsulation material, it is possible to prevent the functional component from losing effectiveness when the encapsulation material is subjected to high-temperature press-forming, thereby solving a problem that effects are poor or costs are high when functional components are directly added to conventional laundry sheets.SELECTED DRAWING: Figure 1

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The invention provides an enzyme protective agent for a continuous glucose sensor and the continuous glucose sensor, the enzyme protective agent comprises the following components: poloxamer and a zwitterionic polymer, and the mass percent concentration of the poloxamer is 0.1%-2%, and the mass percent concentration of the zwitterionic polymer is 0.5%-5%. By adopting the technical scheme provided by the invention, the poloxamer and the zwitterionic polymer are introduced, so that an enzyme layer coating process can be optimized, a coffee ring effect can be inhibited, and the consistency and monitoring accuracy of the sensor can be improved; and on the premise of keeping the signal transmission performance of the sensor unchanged, the activity and long-term stability of the biological enzyme preparation in the production, storage, transportation and use processes are remarkably improved.

The invention discloses a preparation method of an enzyme preparation microcapsule with an intestinal tract slow release function. According to the method, coating wall materials in a specific proportion are adopted, the coating wall materials comprise 10-12 parts of Arabic gum, 4-8 parts of gelatin, 3-6 parts of whey protein, 8-16 parts of beta-cyclodextrin and 5-10 parts of trehalose, the coating wall materials are mixed with 10-15 parts of an enzyme agent, and the enzyme preparation microcapsule is prepared through a spray drying technology. The enzyme preparation microcapsule has excellent thermal stability and gastric juice tolerance, can maintain more than 90% of enzyme activity under the condition of 90 DEG C, and still maintains more than 65% of enzyme activity after being treated in simulated gastric juice for 4 hours. In addition, the enzyme preparation microcapsule has an intestinal directional slow-release function, the enzyme activity is quickly released after entering the intestinal tract for 15 minutes, and the peak value is reached after the enzyme preparation microcapsule enters the intestinal tract for 60 minutes. The method effectively improves the stability and bioavailability of the enzyme preparation in feed processing and animal digestive tract environment, and has good application prospect and market value.

The present invention discloses a method for preparing uric acid-lowering diglyceride by enzymatic hydrolysis, as well as its product and application, belonging to the technical field of diglyceride production. The method comprises the following steps: (1) mixing vegetable oil, glycerol, water, and an enzyme preparation to react to obtain a reaction solution; (2) allowing the reaction solution to stand for stratification and collecting the supernatant; (3) distilling and separating the supernatant, decolorizing, and deodorizing the supernatant to obtain diglyceride; the enzyme preparation in step (1) comprises lipase, esterase, and phospholipase C in a weight ratio of 5-10:0.1-0.3:1. Compared with the existing technology, the method of the present invention is simple and low-cost, and the prepared diglyceride has greater advantages in lowering uric acid.

The invention discloses a neutral phytase mutant as well as a gene and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems that the existing neutral phytase is poor in thermal stability, poor in acidic environment stability, narrow in pH application range, low in low-temperature catalytic efficiency and the like. According to the technical scheme, the phytase preparation PHY06 with high stability and the gene and application of the phytase preparation PHY06 are provided. The method comprises the following steps: carrying out T329G, D333V, T353F and A364F mutation on a PHY03 sequence of which the amino acid sequence is shown as SEQ ID NO.1, so as to obtain a mutant PHY06;

The invention relates to the technical field of biological catalysis, and discloses an M88F mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88F: methionine of the 88th amino acid sequence of UGT76G1 is mutated into phenylalanine; and the substrate rebaudioside A (RA) can be efficiently and directionally converted into rebaudioside I (RI) with higher value. In an optimized reaction system, the conversion rate stably reaches 40%, and the catalytic efficiency is improved by more than 5-8 times compared with the common reference enzyme. The enzyme preparation has the characteristics of high catalytic activity, mild reaction conditions, simplicity and convenience in operation and the like, shows good stability and reproducibility in laboratory research and large-scale production, and has a wide industrial application prospect.

A method for preparing theasinensin A (TSA) by means of a low-temperature enzyme process. The method comprises using a plant polyphenol oxidase enzyme extract or polyphenol oxidase enzyme formulation as an enzyme source, using epigallocatechin gallate (EGCG) as a substrate, using as a reaction system a buffer solution having a certain pH value, and performing an enzymatic reaction at a low temperature and subsequent steps such as column chromatography, distillation and concentration, liquid phase preparation, and freeze drying, thereby synthesizing TSA in a directional enzymatic manner. The method achieves EGCG enzymatic oxidation at a low temperature and obtains dimeric TSA by means of a conversion reaction, laying a theoretical and practical basis for green, safe, efficient and large-scale industrial production and utilization of TSA.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a phosphorylcholine cytidyltransferase mutant and application thereof. On the basis of phosphorylcholine cytidyltransferase MsCCT001, any one or more of N194D, K91E, G98S, S148A and C156V are mutated respectively, a series of phosphorylcholine cytidyltransferase mutants are obtained through screening, and the obtained mutants have the advantages that in the reaction of catalyzing phosphorylcholine and cytidine triphosphate to generate citicoline, the yield of the obtained mutants is increased, and the yield of the obtained mutants is increased. The catalytic efficiency of the compound is 1.90-2.41 times that of initial phosphorylcholine cytidyltransferase, and a biological enzyme preparation with higher catalytic capacity is provided for preparing citicoline by a biological conversion method.

The invention discloses a method for simultaneously producing ergothioneine and an enzyme preparation by utilizing trichoderma reesei, and belongs to the technical field of biological engineering. By optimizing the culture medium, a culture medium formula capable of enabling the trichoderma reesei which has almost no ergothioneine production capacity originally to synthesize the ergothioneine is screened out, and the trichoderma reesei can simultaneously produce the ergothioneine and an enzyme preparation. By further increasing the number of ergothioneine gene clusters of the trichoderma reesei, under the condition of not introducing any exogenous gene, an industrial production optimized strain of the ergothioneine and the enzyme preparation conforming to food safety is constructed, the yield of the ergothioneine and the enzyme preparation can be remarkably improved, and the production cost of the ergothioneine and the enzyme preparation is reduced. Meanwhile, the problems of biological safety, transgenosis safety and the like are solved, and technical support is provided for industrial production of ergothioneine and enzyme preparations.

The invention discloses an enzyme preparation for catalytic synthesis of D-chiro-inositol and a preparation method of D-chiro-inositol, and belongs to the technical field of genetic engineering. The enzyme preparation for catalytic synthesis of D-chiral inositol has enzyme activity for catalyzing the reaction of muscle inositol and NADP < + > to generate D-chiral inositol, and comprises inositol dehydrogenase and keto isomerase, the amino acid sequence of the inositol dehydrogenase is shown as any one of SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and the amino acid sequence of the keto isomerase is shown as any one of SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8. The invention further discloses a preparation method of the enzyme preparation for catalytic synthesis of D-chiral inositol. The inositol dehydrogenase and the keto isomerase in the enzyme preparation are mutated on the basis of wild types, so that the enzyme activity of the inositol dehydrogenase and the keto isomerase is improved, and the conversion rate of D-chiral inositol is further improved.

The invention relates to the technical field of sludge treatment and recycling, in particular to a novel sludge conditioner for a novel filter press, which is prepared from the following components in percentage by weight: 15 to 30 percent of modified humate, 5 to 12 percent of compound enzyme preparation, 8 to 20 percent of zwitterionic polymeric flocculant, 20 to 40 percent of inorganic skeleton construct, 3 to 10 percent of oxidant and 10 to 25 percent of hydrophobic modified mineral particles. And the balance of pH regulator and deionized water. According to the novel sludge conditioner disclosed by the invention, by compounding modified humate and other core components, a synergistic action mechanism is constructed, so that the water content of a sludge filter cake is less than or equal to 50-60%, the weight of the filter cake is reduced by over 20%, the COD (Chemical Oxygen Demand) of filtrate is reduced by over 30%, the novel sludge conditioner is adaptive to various filter presses, the filter pressing period is shortened by over 15% through an optimized conditioning process, the productivity is improved, and the treatment cost and the environmental protection pressure are reduced.

The present invention belongs to the field of tobacco products and relates to a method for evaluating the effectiveness of an enzyme preparation in improving the quality of tobacco leaves, including: applying the enzyme preparation to tobacco leaves of a certain grade; detecting the tobacco leaves of the certain grade, the treated tobacco leaves of the certain grade and the tobacco leaves of a higher grade by Raman spectroscopy to obtain Raman spectral curves; selecting a Raman shift range of 800-1800 cm ‑1 on the curve, connecting the two ends to obtain a straight line segment; subtracting the intensity corresponding to the straight line segment from the intensities corresponding to multiple Raman shifts on the curve segment to obtain a data set I R , dividing by the maximum intensity I max on the curve segment to obtain a data set I; calculating the first root mean square error of the data set I between the treated tobacco leaves of the certain grade and the tobacco leaves of the certain grade, and the second root mean square error of the data set I between the treated tobacco leaves of the certain grade and the tobacco leaves of a higher grade; if the former is greater than the latter, it is effective in improving the quality of tobacco leaves, otherwise it is ineffective. The present invention also relates to the application of the enzyme preparation in improving the quality of tobacco leaves. The method of the present invention can quickly and accurately evaluate the effectiveness of the enzyme preparation in improving the quality of tobacco leaves.

The present invention discloses a transaminase and its use in preparing optically pure chiral amines. The amino acid sequence of the transaminase of the present invention has one or more mutations at positions 65, 318, 399, 419, 426, 465, and 466 compared to SEQ ID NO: 1, wherein the mutation is an addition, deletion, or substitution of an amino acid residue. The present invention also discloses a nucleic acid molecule encoding the transaminase, a nucleic acid construct comprising the nucleic acid molecule, a recombinant vector, and a host cell. The present invention also discloses an enzyme preparation comprising the transaminase of the present invention. The present invention also discloses a method for preparing chiral amines using the transaminase. In the industrial production process of optically pure chiral amines, the enzyme provided by the present invention has advantages such as substrate specificity, enantiomeric selectivity, and high conversion rate. The method for preparing optically pure chiral amines provided by the present invention has high reaction efficiency, stereoselectivity, and yield.

The utility model discloses a convenient on-line enzyme preparation adding device which comprises a wort conveying pipe, one side of the surface of the wort conveying pipe is fixedly communicated with an inlet pipe, one end of the inlet pipe is fixedly communicated with a mixing tank, one end of the mixing tank is fixedly communicated with an outlet pipe, and one end of the outlet pipe is fixedly communicated with the other side of the surface of the wort conveying pipe. One side of the top end of the mixing tank is fixedly communicated with an adding pipe, the top end of the adding pipe is fixedly connected with a funnel, a screen mesh is clamped at the top of the inner wall of the funnel, the other side of the top end of the mixing tank is fixedly communicated with a drainage pipe, and a stirring mechanism is arranged in the mixing tank. After addition, the enzyme preparation is brought into the fermentation tank through wort circulation, so that the enzyme preparation is convenient to add and free of residues, the stirring mechanism is arranged, the enzyme preparation and the wort can be rapidly and fully mixed, the decomposition efficiency is improved, and the adding device is convenient to clean.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The utility model provides a precipitation integrated biological enzyme treatment device, and belongs to the technical field of purification and separation equipment. Comprising a first tank body, a top cover is arranged at the top of the first tank body, a mounting base is coaxially and rotatably arranged in the first tank body, a second tank body is detachably connected to the mounting base, a stirring assembly, a liquid inlet pipe, a feeding port and an extraction assembly are arranged on the top cover, a plurality of liquid drainage holes are formed in the side wall of the second tank body in a penetrating mode, and filtering layers are arranged in the liquid drainage holes; a plugging assembly for sealing the filter layer is arranged in the second tank body, and a liquid discharge pipe is arranged at the bottom of the first tank body. And carrying out precipitation treatment in the second tank body, after the treatment is completed, extracting supernatant liquid, then opening the plugging assembly, driving the second tank body to rotate, and carrying out centrifugal separation to obtain separated enzyme-containing precipitates. The method has the effect of improving the yield of the enzyme preparation.

The utility model discloses an efficient precipitation separation device for an enzyme preparation, and relates to the technical field of enzyme preparation processing. An organic solvent such as acetone or ethyl alcohol and an enzyme preparation are injected into the medicine box through the feeding pipe, after the driving machine is started, the driving wheel on the outer side of the driving machine can conduct circular motion around a circle, and therefore the limiting plate and the movable column can conduct circular motion in the vertical direction; furthermore, the medicine box can do reciprocating motion in the box body in the vertical direction, precipitation of the enzyme preparation can be accelerated under the cooperation of the organic solvent, and the subsequent effect is guaranteed; after the motor is started, the separation barrel can be driven to do circular motion, so that liquid medicament in the separation barrel can be centrifuged, the purpose of efficient separation can be achieved, and when the separation barrel rotates, the limiting seat synchronously does circular motion on the inner bottom wall of the box body, so that effective supporting of the separation barrel can be achieved, and inclination or shaking is avoided; and the actual use effect can be ensured.

The invention relates to the field of enzyme engineering, and provides a BhrPETase variant capable of improving stability through site-specific replacement under the background of Bhr6M (SEQ ID NO: 1) and application of the BhrPETase variant, and the replacement is M57I and / or S184H, preferably double mutation M57I / S184H. The variant shows remarkable thermal stability and storage stability under a unified measurement caliber: the condition t1 / 2 at 90 DEG C is greater than or equal to 400 minutes, the condition t1 / 2 at 70 DEG C is greater than or equal to 7 days, and the residual activity after 30 days of storage at 30 DEG C is greater than or equal to 90%. The invention also discloses a nucleic acid encoding the variant, a vector, a recombinant host, an enzyme preparation, and a method for depolymerizing PET and a copolymer thereof in a process temperature range of 70-90 DEG C. Compared with a control Bhr6M, the variant maintains more lasting activity in high-temperature and long-period operation, is suitable for PET substrates with different forms and crystallinity, reduces the enzyme supplementing frequency and improves the stability of a device.

The invention discloses an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. The enzyme-containing anti-inflammatory composition comprises an enzyme preparation, a hair follicle maintenance agent, a functional auxiliary agent and a carrier raw material. The core innovation lies in that a hair follicle targeted enzymolysis technology is adopted, and a specific enzyme modified by polyethylene glycol can efficiently dredge hair follicles; the anti-inflammatory and hair follicle repairing dual effects are achieved through anti-inflammatory-maintenance synergistic matching and adding sequence optimization; the damaged barrier is rapidly repaired by combining a composite barrier repairing agent and a gradient permeation process; enzyme activity and mildness are guaranteed by adopting a microcapsule embedding and low-temperature preparation technology. The composition can specifically solve the problems of hair follicle blockage, inflammation and unhairing, is suitable for pets of all ages and skin types, and is high in practicability.

The invention relates to the technical field of oxygen free radical detection, in particular to a manufacturing process of a portable and reliable oxygen free radical detector. Comprising the following steps: mixing superoxide dismutase, potassium ferricyanide, an isothiazolinone preservative, hexammine ruthenium chloride, polyethylene glycol octylphenol ether, trehalose and bovine serum albumin, and uniformly stirring to prepare an enzyme preparation; mixing an enzyme preparation with the high polymer material solution to form reagent layer slurry with viscosity; uniformly coating the reagent layer slurry on a substrate material, and drying; printing an electrode material on the substrate material to form a working electrode and a counter electrode; the silver electrode is placed in an electrolyte containing chloride ions, potential is applied, the silver surface is subjected to a chlorination reaction, and a silver / silver chloride electrode is formed; covering the reagent layer above the electrode, tightly combining the reagent layer with the electrode in a sticking or hot pressing manner, and cutting to obtain the test paper; the prepared portable oxygen free radical detector can be used for conveniently and efficiently detecting oxygen free radicals of a human body at low cost.

A preparation method of N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate enzyme belongs to the field of medical intermediates, and comprises the following steps: step 1, preparing a buffer solution of a compound enzyme preparation; step 2, adding (2S)-2-benzyloxy-3-pentanone into a solvent A, and then adding a buffer solution of the compound enzyme preparation for reaction; 3, filtering, and carrying out an activation reaction on the filtrate; 4, filtering, removing the solvent, adding the obtained solid into a solvent B, and then adding formylhydrazine for reaction; 5, filtering, removing the solvent, adding the obtained solid into a solvent C, and then adding oxalic acid for reaction; and 6, drying the solid obtained by filtering to obtain the N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate. According to the method, the yield of the N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate can be increased, and the production cost is effectively reduced.