814 results about "Enzyme agent" patented technology

A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.

Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.

The invention relates to a sucking pig compound feed and a preparation method thereof. The feed comprises the following raw materials in parts by weight: 30-90 parts of vegetable feed raw material, 25-80 parts of vegetable puffed feed, 5-25 parts of fermentation bean pulp, 2-10 parts of fish meal, 6-30 parts of dairy product, 1-4 parts of oil, 0.2-3 parts of amino acid, 0.01-0.05 part of liquid additive and 1-3 parts of solid additive. The preparation method of the feed provided by the invention can ensure the best use of the nutrient components of different materials, can overcome the instability of enzyme agents and microbial ecological agents caused by heating, and can effectively eliminate the indigestion caused by hyposecretion of digestive enzymes before and after the weaning of thesucking pig and improve the intestinal canal health as well as the pig immunity. Through the sucking pig compound feed provided by the invention, the problem of weaning stress of the sucking pig is solved; and compared with the common piglet feed, the morbidity is greatly reduced, the feed intake and daily gain are increased, and the material-meat ratio, morbidity and mortality are reduced.

The invention relates to a detergent and a preparation method thereof and particularly provides a tableware detergent with enzyme and a preparation method thereof. The raw materials of the detergent comprise the following components: surfactant, enzyme preparation, enzyme stabilizer, preservative, deionized water and the like. The tableware detergent with enzyme provided by the invention can maintain higher enzyme activity during the service life of the detergent and can remove stubborn marks and stains on the tableware better at room temperature which are hard to remove in the condition of not hurting the surface of the porcelain, glass and metal and decorations. The preparation method provided in the invention is easy and feasible and is applicable to industrialized production.

The invention discloses a bio-organic fertilizer which is prepared from the following components in parts by weight: 800-820 parts of agricultural wastes, 12-18 parts of calcium superphosphate, 4-6 parts of potassium mineral powder, 4-6 parts of fermenting enzyme agent, 40-60 parts of mineral powder, 90-110 parts of boron soil powder, 8-12 parts of humic acid and 4-6 parts of culture. The preparation method comprises the following steps: uniformly mixing all raw materials, adding water for uniformly stirring, stacking the materials into cubic piles at the temperature of more than or equal to 15 DEG C, and making air holes on the cubic piles; turning for 1-2 times per day when the temperature of the material piles reaches 50 DEG C, turning for 4-5 times per day when the temperature of the material piles is more than or equal to 65 DEG C, controlling the fermentation temperature to be 70 DEG C or below; and preparing the fertilizer when the materials in the material piles are loose and the original odor of the materials is avoided. The bio-organic fertilizer has the advantages that the bio-organic fertilizer is full in nutrient elements and is green and environment-friendly, the soil can be improved, and soil hardening caused by use of the fertilizer is improved.

The invention relates to a kind of low-sugar and low-fat biscuit and the preparation method. The method comprises following steps: preparing flour dough with yeast and medium-strength (or high-strength)flour, getting fermented flour dough, adding egg, sugar and oil and other findings into flour dough, mixing with fermented flour dough and enzyme agent, adding low-strength flour, shaping through roll marking, cutting or roll cutting, drying, cooling and getting finished product. The invention employs low-sugar and low-fat formulation, combines biological techniquie such as fermentation and enzyme agent and produces crisp biscuit.

The invention relates to a cooperative pretreatment method for biological catalysis and controlled oxidation of cotton and cotton-type fabrics, comprising the following steps: (1) preparing a biological compound enzyme preparation, a biological enzyme synergist and a non-ionic surfactant into an enzyme working solution, wherein the pH of a processing bath is 6-7.5; padding the fabrics into the working solution; carrying out heat preservation; (2) washing the processed fabrics with cold water; padding the fabrics into a hydrogen peroxide bleaching solution containing the controlled decomposition additive of hydrogen peroxide; and steaming, washing with water and drying to obtain the finished products. In the method, high-density strong base is not required to serve as a scouring agent, thepH value of pretreatment waste water is 7-8, the chromaticity is low, the COD (Chemical Oxygen Demand) value is lowered by 30% compared with that of the traditional alkali kiering pretreatment technology, the water is saved by 10-30%, and the steam and electricity can be saved by 20%. The indexes (whiteness, capillary effect, water absorption, cotton seed hull strainaway rate, desizing rate, strong force and the like) of the processed cotton and cotton-type fabric all reach the quality indexes of a semi-product, cotton seed hulls can be completely removed, and the fabrics have vivid color andgood levelling property after being dyed.

The invention relates to the genetic engineering field, especially to a strain Bispora sp.MEY-1 which produces an acidophilic beta-mannanase and the acidophilic beta-mannanase MAN5A got from the strain having an amino acid sequence shown as SEQ ID NO.1 or 2, a gene man5A for coding said beta-mannanase having a nucleotide sequence shown as SEQ ID NO.1 or 2, and a recombinant vector containing said gene and applications. The beta-mannanase of the invention has the following advantages: the optimum pH 1.0-1.5, the optimum temperature 65 DEG C, good pH stability and thermostability, high specific activity, good protease resistance and easy for industrial fermentation production. The product of the invention can be widely used for the animal feeding-stuffs, the food, the medicament, the brewing and the energy industry as a novel enzyme preparation.

The invention provides an enzyme preparation for degumming fresh ramie, a preparation method thereof and a method for degumming the fresh ramie. Alkaline pectinase and hemicellulase are mixed, and the obtained enzyme preparation can be directly used for the method for degumming the fresh ramie, so that the problem that the fresh ramie cannot be effectively degummed by a chemical degumming method and a biological bacterium degumming method is solved; meanwhile, the process for degumming the fresh ramie is simple, and preparation and enzyme immersion steps of the fresh ramie are performed in a farmland; the using amount of chemical medicaments in high-pressure cooking treatment fluid is small, and toxic chemicals are not used; and the chemical oxygen demand in wastewater obtained after ramie fibers are prepared is low, and the wastewater contains a large number of ions such as phosphorus, potassium, sodium and the like, so the wastewater can be directly used for irrigating the farmland, and the cost is saved and the environment is also protected.

The invention relates to application of an alpha-L-rhamnoside enzyme in the directional synthesis of isoquercitrin by the biological conversion of rutin. Because the crude enzyme preparation or the immobilized enzyme preparation of the alpha-L-rhamnoside enzyme is used for catalyzing and hydrolyzing the rutin and directionally and biologically synthesizing the isoquercitrin, the application has the advantages of wide source, easy preparation and low cost of catalysts, high stability, and high catalysis efficiency and specificity of the enzyme preparation, and is easy to store. Thus, the alpha-L-rhamnoside enzyme can greatly reduce the production cost of quercetin, the catalysis and conversion rate of the enzymes is high, and the products are single.

The invention discloses a bio-enzyme degerming and cleaning effervescent tablet for clothes. The bio-enzyme degerming and cleaning effervescent tablet for the clothes is prepared from the following components in percentage by weight: 20 percent to 55 percent of alkaline effervescing agent, 15 percent to 40 percent of surface active agent, 10 percent to 30 percent of acid effervescing agent, 2 percent to 10 percent of adhesion agent, 1 percent to 5 percent of complex enzyme preparation, 0.5 percent to 3 percent of decolorizer, 0.5 percent to 5 percent of disintegrating agent, and 0.5 percent to 2.5 percent of enzyme stabilizer. The bio-enzyme degerming and cleaning effervescent tablet for the clothes is good in cleaning effect, fast in effervescence time and intense in sense effect; when being used for cleaning the clothes, the components of the bio-enzyme degerming and cleaning effervescent tablet for the clothes are synergized, and especially the enzyme preparation, the surface active agent and various additives are synergized, so that various stubborn stains in the clothes can be quickly removed, the surface active agent has no residue after cleaning, and the bio-enzyme degerming and cleaning effervescent tablet for the clothes is healthy and safe.

The invention discloses an enzyme-bacterium duplex straw decomposing agent and its preparation and application methods. The decomposing agent is made by mixing a composite microbial agent and a composite enzyme agent in a weight ratio of 3-4:1. The composite microbial agent includes Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Brevibacillus laterosporus and a fused Saccharomyces cerevisiae, having a weight ratio of (1-2):(1-2):(1-2):(2-3):(2-3). The composite enzyme agent includes cellulase produced by the fermentation of Trichoderma reesei, hemicellulase produced by the fermentation of Bacillus subtilis, and ligninase produced by the fermentation of Pleurotus ostreatus, having a weight ratio of (1-2):(1-2):(1-2). The decomposing agent has a double straw degrading function, allowing more efficient and more thorough biological degradation, increasing the straw degrading speed to a higher extent and shortening degrading time, and can fully decompose crop straw to fields within about a week, without affecting the planting and transplanting of next-stubble crops.

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergilllus host. In a specific example, the specific performance of the formulation using puriified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

The invention discloses a compound enzyme for bear, and belongs to the field of enzyme preparation processing. The compound enzyme adopts a natural concentrated maltase as a main material, and is complexed with a mixed enzyme scientifically, so that not only are full enzyme sources filled, but also more importantly under the synergistic effect of a protective agent and an activating agent, the enzyme activity is enough, the storage performance is good, a nitrogen source is rich, the malt flavor is strong, and the loss rate of the enzyme activity after 2-year storage at normal temperature is less than 3%. The compound enzyme disclosed by the invention has the advantages that the complexing is scientific, the preparation process is simple, the function of a product is complete, the storage period is long, the transportation, the storage and the use are convenient, especially a solid foundation is laid for carrying out added enzyme-preparation saccharification with the auxiliary-material ratio, reducing the production cost of the bear and producing the high-quality traditional bear with rich nutritional values and natural-pure taste and flavor.

The invention discloses a preparing method of resistant malt dextrin, which comprises the following steps: adopting starch as raw material; generating dextrin through acid heat; hydrolyzing through biological enzyme agent; decolouring through activated carbon; desalting through ionic exchanging resin; spraying and drying to obtain the product.

The present invention relates to enzyme preparation, and is especially one kind of composite enzyme preparation for degrading agricultural waste effectively and its preparation process. The composite enzyme preparation includes xylanase with enzyme activity not lower than 100 KU / g, beta-dextranase with enzyme activity not lower than 80 KU / g, cellulose with enzyme activity not lower than 28 KU / g, acid protease with enzyme activity not lower than 56 KU / g, lipase with enzyme activity not lower than 15 KU / g, pectase with enzyme activity not lower than 6 KU / g, and alpha-amylase with enzyme activity not lower than 12 KU / g. Its preparation process includes the steps of spawn preparation, enzyme preparation production, compounding, etc. The present invention may be used in feed production, methane production and other fields.

The invention provides an anti-aging method for deep-fried glutinous rice cake stuffed with bean paste, which belongs to the technical field of agro-product processing and food additive. The invention mainly relates to preparation of an anti-aging compound emulsifying agent and anti-aging application of the compound emulsifying agent and an enzyme agent to the deep-fried glutinous rice cake stuffed with bean paste. In the invention, glutinous rice flour is taken as a main raw material, certain amount of the compound emulsifying agent, the enzyme agent, sugar and water are added, and the product, namely deep-fried glutinous rice cake stuffed with bean paste is produced by stewing and forming processes. The compound emulsifying agent is mainly produced by an extrusion method. A plurality of powder emulsifying agents with good anti-aging effect is evenly distributed on the surface of starch particles. The compound emulsifying agent with good anti-aging effect is obtained by controlling extrusion parameters. The optimal adding amount of the compound emulsifying agent is between 0.2 and 3 percent. The enzyme agent mainly adopts maltose amylase with the adding amount of between 0.02 and 0.15g / kg. The adding amount of the water and the sugar is respectively 110 to 120 percent and 20 to 50 percent of the weight of the glutinous rice flour. Through adding the compound emulsifying agent and the enzyme agent, the deep-fried glutinous rice cake stuffed with bean paste has good anti-aging function, and ensures that the guarantee period is more than 60 months.

The invention provides a steam-explosion combined bio-composite enzyme preparation, and a method for producing ginsenoside by using ginseng, American ginseng, ginseng stems and leaves, American ginseng stems and leaves and the like as raw materials. Compared with a conventional method, the method has the characteristics of high yield of ginseoside extract, high content of extract saponin, high extraction rate of gnsenoside and the like.

The invention discloses a soil conditioner for acidified land. The soil conditioner for the acidified land is prepared from the following materials in parts by weight: 50 to 100 parts of fulvic acid, 80 to 150 parts of diatomite, 50 to 100 parts of charcoal, 1 to 5 parts of biological microbial agent, 1 to 5 parts of biological enzyme agent, 80 to 150 parts of illegal cooking oil, 50 to 80 parts of kitchen waste, 20 to 50 parts of lime powder, 30 to 60 parts of bentonite, 30 to 60 parts of decomposed pig manure, 50 to 100 parts of bagasse and the like. Weakly-alkaline amino acid chelating fluid added into the soil conditioner and aluminum ions of acidified soil effectively form a chelate, and thereby ionic aluminum is significantly reduced; at the same time, papermaking waste liquid and the illegal cooking oil are also added into the soil conditioner, the papermaking waste liquid is alkaline and can generate organic matter by fermentation to be absorbed by the soil, and nutrients and the pH value of the soil are improved.

The present invention provides an enzyme preparation containing, as the active ingredient, an acidic or alkaline substance capable of shifting the pH value of the preparation toward a pH range wherein the expression of activity of a transglutaminase is inhibited when the transglutaminase is dissolved in the presence of collagen in a solution. The present invention also provides a process for producing a food by using the aforementioned enzyme preparation.

The invention discloses a feed fermentation agent and a fermented feed, the feed fermentation agent includes the following components by mass: 20-25% of pichia pastoris, 15-20% of lactobacillus, 10-15% of enterococcus faecalis, 3-10% lactobacillus plantarum, 10-15% of cellobiose lactobacillus and 15%-25% a compound enzyme agent. The fermented feed is a fermented product of soybean meal, corn and wheat bran, mulberry-rich plants and a mixture of mulberry-rich plants and soybean meal by use of the feed fermentation agent, after fermentation of the soybean meal or corn or wheat bran and other traditional feed by use of the feed fermentation agent, harmful materials such as accumulated stool and the like cumulated in pig intestines can be decomposed and removed by cooperation of crude fiber in the traditional feed and microorganism, so that the intestinal absorption efficiency of nutrients can be provided.

The present disclosure relates to enzyme formulations and methods of using the enzyme formulations to reduce the viscosity of fluids used in hydrocarbon recovery. Some embodiments provide particles for well treatment, where the particles comprise an acidifier carrier and an enzyme co-encapsulated within a shell. The particles can, for example, allow a delayed or controlled release of the enzyme in a high temperature, high pressure environment.

The invention relates to the field of the genetic engineering, and in particular relates to xylosidase Xyl43B with high xylose tolerance, and a gene and an application thereof. The invention provides xylosidase Xyl43B which is from humicola sp.L8, and an amino acid sequence of xylosidase Xyl43B is shown as SEQ ID No.1. The invention provides a coding gene xyl43B for coding xylosidase. The xylosidase has the following properties that the xylose tolerance is high (ki value is 292mM), the xylosidase is stable at the preferable condition that pH is 7.0 and between 5.5 and pH 10.0, and the preferable temperature is 50DEG C and xylosidase has good thermal stability at the temperature of 50DEG C. As a novel zymin, the xylosidase can be widely applied to the food, feed and energy industries.

The present invention relates to the field of microbial engineering technology. The enzyme preparation is prepared through the process including slant culture and storing, shaking culture, seeding tank culture and fermenting tank production of high enzyme activity bacteria strain. Bacillus subtilis of No.13 strain and preservation number CCTCC No.M200038 is selected. The hemp degumming process includes the fermentation of enzyme liquid filtering residue, diluting bacteria liquid to 3-6 times, regulating the pH value of fermented enzyme liquid in degluing tank to 7.5-9.0, hemp material pre-treatment, degumming with degumming liquid for 2-10 hr. The process of the present invention has no environmental pollution, and can produce hemp top with length, fineness and breaking strength superior to those of chemical process.

The invention relates to a method for preparing the biological enzymed corn rice powder, which comprises that immerging the corn or corn slag, using alkali liquid to disinfect, exchanging the immerge solution, adjusting pH value, adding enzyme agent and galactenzyme to process the biological enzyme for 40-50h, taking out the enzyme corn or corn slag, spraying water, dewatering eccentrically at high speed, drying, breaking fermented corn or corn slag, mixing it with rice powder and modified amidon, compressing the aged corn rice bar via high temperature and high pressure, breaking, screening the 100-200 deals, to prepare the final product with strong viscoelasticity. The invention powder can be used to prepare cake, or the like, which is abundant in food fiber.

The present invention relates to a low protein laying hen concentrated feed with amino acid balance. The feed has the following advantages that: the animal digestive utilization rate is high, the flora balance in the digestive tract can be effectively maintained, the cost is low, the resource is saved, and the environmental pollution is reduced. The feed of the present invention is prepared by the following raw materials, wherein the raw materials comprise, by weight, 320-390 parts of soybean meal, 100-140 parts of cottonseed meal, 30-70 parts of rapeseed meal, 65.4-105.4 parts of corn pulp protein, 20-60 parts of corn protein powder, 235-275 parts of calcium carbonate, 28-36 parts of calcium hydrogen phosphate, 0.8-1.4 parts of a compound vitamin, 1.2-1.7 parts of compound trace element,13-17 parts of grease, 6.5-10.5 parts of lysine, 2-6 parts of liquid methionine, 0.8-1.2 parts of threonine, 6.5-10.5 parts of salt, 0.3-0.7 parts of an antioxidant, 1-5 parts of sodium bicarbonate, 1.2-1.7 parts of a microecology agent, 0.2-0.6 parts of phytase, 0.3-0.7 parts of a complex enzyme agent and 1-3 parts of choline choride.

The invention discloses a soil improvement agent for a saline-alkali land. The soil improvement agent is prepared from the following raw materials in parts by weight: 100-200 parts of humic acid, 50-100 parts of algae mud, 50-100 parts of biochar, 1-5 parts of a biological fungicide, 1-5 parts of a biological enzyme agent, 50-100 parts of pine needle meal, 50-100 parts of brewer's grain, 20-50 parts of sulfur slag, 20-50 parts of bentonite, 100-200 parts of chicken manure, 30-50 parts of straw, 20-30 parts of feather meal and 20-30 parts of earthworm powder. The sulfur ions and sulfuric acid contained in the added sulfur slag are firstly separated out by hot water, are attached to the biological fungicide by virtue of the biological fungicide and then are bound with ions in the saline-alkali land. By further adding an acidic amino acid chelating solution and a bamboo vinegar solution, the pH value of the saline-alkali land is decreased; and by adding the biochar, coconut chaff and rice bran, moisture and water preservation effects are achieved, and soil erosion is prevented.

The invention discloses a production method of a heat-resistant xylanase that utilizes a thermophilic fungus DSM10635 and the usage of the heat-resistant xylanase in feedstuff. As a feedstuff addition agent, the heat-resistant xylanase can be directly used or mixed with other enzymes so as to prepare a composite enzyme agent. The heat-resistant xylanase production method has following advantages: a mixture of dry corncob powder and flour water is used as a culture medium, thus being very simple and cheap; processing of raw materials is simple; product extraction is simple, product can be obtained by crashing materials after solid fermentation, and the whole process does not have waste discharge; the culture medium contains little water and consequently has reduced chances of bacteria contamination.

The invention relates the method for producing mature vinegar, comprising the following steps: adding biochemistry enzyme agent and microbial population, and adjusting temperature. The invention has the advantages of shortening production circle and reducing cost.

A process using a multicomponent enzyme preparation to treat screened once refined pulps and reduces the specific energy consumption and / or increasing production while maintaining or increasing handsheet physical properties. The enzyme preparation has a major endoglucanase activity, a significant mannanase activity and a relatively small cellobiohydrolase activity. This enzyme mixture is prepared from a genetically modified strain of Trichoderma reseii.