842 results about "Enzyme agent" patented technology

The invention relates to a chitosanase mutant capable of changing the polymerization degree of a product, a plasmid, a recombinant bacterium and application of the chitosanase mutant, and belongs to the technical field of enzyme engineering, the mutant is Csn-BJ-A134C or Csn-BJ-Q97K, the amino acid sequence of the mutant Csn-BJ-A134C is SEQ ID NO: 1, the amino acid sequence of the mutant Csn-BJ-Q97K is SEQ ID NO: 2, and the amino acid sequence of wild chitosanase Csn-BJ is shown as SEQ ID NO: 3. The invention also provides a recombinant vector, a recombinant bacterium and an enzyme preparation containing the mutant gene, and application of the recombinant vector, the recombinant bacterium and the enzyme preparation. The mutant disclosed by the invention is derived from GH46 family chitosanase Cs-BJ, molecular mutation modification is performed on the chitosanase, so that the catalytic activity of the chitosanase is improved, the polymerization degree of a final product chitosan oligosaccharide is changed, the polymerization degree of a minimum hydrolysis substrate is increased, and a foundation is laid for industrial application of the mutant.

The invention relates to the technical field of fermentation monitoring, in particular to a fermentation monitoring method, device and equipment. The method comprises the following steps: acquiring adjustment equipment information, equipment application data and real-time environment information, processing the adjustment equipment information, the equipment application data and the real-time environment information to obtain an equipment monitoring model, and performing application monitoring on each piece of environment regulation and control equipment based on the equipment monitoring model, judging whether at least one environment regulation and control device is abnormal in application or not, if yes, performing abnormality analysis on the at least one environment regulation and control device based on the device monitoring model and the real-time environment information to obtain an abnormal device set and a weight value corresponding to each abnormal device in the abnormal device set, and binding and marking the abnormal equipment set and the weight value according to the equipment structure position and the equipment monitoring model, and controlling and displaying the equipment monitoring model after binding and marking. The fermentation efficiency of the enzyme preparation fermentation process is improved.

The invention relates to the technical field of production monitoring, in particular to a method, a system and equipment for monitoring key links of enzyme preparation production. The method comprises the following steps: acquiring equipment node information of an enzyme preparation production key link and equipment operation information corresponding to the equipment node information, and determining set operation parameters corresponding to each production equipment node in the production key link based on the equipment node information, determining an actual operation parameter corresponding to each production equipment node based on the equipment operation information, and performing backtracking calibration on the set operation parameter according to the actual operation parameter and a preset calibration algorithm to obtain a calibration operation parameter corresponding to each production equipment node, and determining whether the calibration operation parameters are consistent with the set operation parameters based on the production equipment nodes, if not, generating a parameter calibration instruction based on the calibration operation parameters and the set operation parameters, and controlling the production equipment corresponding to the set operation parameters to perform parameter calibration. The production quality of the enzyme preparation is improved.

The invention relates to an intelligent regulation and control method and system for fermentation parameters, a terminal and a medium, and belongs to the technical field of enzyme preparation fermentation. The intelligent regulation and control method for the fermentation parameters comprises the following steps: determining a current fermentation stage of a fermentation process; determining a dissolved oxygen target value and a pH target value according to the fermentation stage; acquiring monitoring data, wherein the monitoring data comprises a dissolved oxygen actual value, a dissolved oxygen change rate, a pH actual value, a pH change rate, a thallus concentration and a fermentation volume; if the change rate of the dissolved oxygen is a negative value, predicting a pH predicted value in future n minutes according to the actual value of the dissolved oxygen and the thallus concentration; judging whether an error value between the pH predicted value and the pH target value is not greater than an error threshold value or not; if yes, parameter intervention is carried out, and parameters comprise the ventilation capacity, the stirring speed and the alkali supplementing amount. The method has the beneficial effects that the fermentation parameters can be precisely regulated and controlled, so that the quality of a fermentation product is precisely improved.

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention relates to an automatic temperature control type concentration device for an industrial biological enzyme preparation, in particular to the technical field of enzyme preparation purification devices. The technical problem that a heating plate cannot conduct targeted heating on the position where enzyme-containing liquid is located is solved. Comprising a support, a heating shell is slidably connected to the interior of the support, a liquid storage tank is fixedly connected to the interior of the heating shell, a hot air pump is installed on the support, the hot air pump is fixedly connected with a first air conveying pipe, the first air conveying pipe is fixedly connected and communicated with the heating shell, and a sealing ring is slidably connected to the interior of the heating shell in a sealed mode. The sealing ring is connected with the outer side of the liquid storage tank in a sealed and sliding mode. The position of the sealing ring is changed along with the change of the liquid level of the stock solution in the liquid storage tank, so that the volume of hot air stored between the heating shell and the liquid storage tank is changed, the heating position of the liquid storage tank is further changed, the volume of the stock solution in the liquid storage tank is heated, and waste of the hot air is avoided.

The invention relates to the field of enzyme preparations, and discloses an enzyme preparation for catalytic degradation of bioplastics and a preparation method thereof, the enzyme preparation for catalytic degradation of bioplastics comprises the following components by weight: 25-50 parts of an enzyme, 1-8 parts of a catalytic additive, 2-10 parts of a stable dispersant, and 30-50 parts of a buffer solution; the catalytic additive is prepared by using calcium bentonite as a carrier to load nano titanium dioxide; the stabilizing dispersant is prepared by introducing polyethylene glycol monoallyl ether into a sodium lignin sulfonate structure by utilizing an initiator through an aqueous solution polymerization method, and by adding the catalytic additive and the stabilizing dispersant, the enzyme preparation is endowed with good degradation efficiency on bio-based plastics and the stability of an enzyme preparation system is improved.

The invention belongs to the technical field of biology, and discloses a halohydrin dehalogenase mutant, a coding gene, a recombinant vector, a recombinant strain, an enzyme preparation and application of the halohydrin dehalogenase mutant, the coding gene, the recombinant vector, the recombinant strain and the enzyme preparation in synthesis of chiral (S)-5-phenoxymethyl-2-oxazolidinone compounds. The halohydrin dehalogenase mutant is obtained by performing combined mutation on an enzyme with an amino acid sequence as shown in SEQ ID NO.1 at the 15th site, the 137th site and the 179th site. The mutant can obviously improve the ee value of a chiral (S)-5-phenoxymethyl-2-oxazolidinone compound synthesized by catalyzing phenyl glycidyl ether, the purity of catalytic synthesis and resolution is higher, and industrial production is facilitated.

The invention belongs to the technical field of kitchen waste treatment, and particularly relates to a resource recovery method for strengthening rapid enzyme hydrolysis of kitchen waste and synchronously producing a solid organic fertilizer and a denitrification carbon source. Alkaline substances (namely plant ash and biochar) are added in the enzyme hydrolysis process of the kitchen waste, hydrolysate with high organic matter concentration and excellent storage performance is produced and directly used as a denitrification carbon source, and corresponding hydrolysis residues have high nutrient content and can be directly converted into ecological organic fertilizer. Based on a self-made bio-enzyme preparation technology, alkaline substances are doped into a kitchen waste hydrolysis system, the purpose of efficient resource recovery of kitchen waste is expected to be achieved, and the effect of treating waste with waste is achieved.

The invention discloses a ganoderma lucidum extraction method based on a reusable magnetic enzyme. The ganoderma lucidum extraction method comprises the following steps: S1, preparing a nanoscale amination modified Fe3O4-SiO2 magnetic composite carrier; s2, on the basis of the amination modified Fe3O4-SiO2 magnetic composite carrier, cellulase is added, and a magnetic immobilized enzyme buffer solution is obtained; s3, the ganoderma lucidum sporocarp is put into a magnetic immobilized enzyme buffer solution to be subjected to enzymolysis, extraction and filtration, effective components of ganoderma lucidum are obtained, and magnetic immobilized enzyme is recycled for repeated utilization; according to the method, the magnetic nanoparticles are modified, so that the surfaces of the magnetic nanoparticles have specific active groups, meanwhile, the magnetic response advantage of an inorganic magnetic material is reserved, the nanoscale amination-modified Fe3O4-SiO2 magnetic composite carrier is prepared, enzyme molecules can be directly anchored on the surfaces of the magnetic nanoparticles, and enzyme activity is improved. The immobilized enzyme can be conveniently recycled from a reaction system, so that the cost of an enzyme preparation can be reduced, and the enzyme activity stability can be improved.

The invention belongs to the technical field of feed additives, and particularly provides a compound preparation for improving the digestibility of dry coconut shreds, which comprises a compound enzyme preparation, the compound enzyme preparation comprises xylanase with the enzyme activity of 0.5 million U / g to 25 million U / g, glucanase with the enzyme activity of 0.8 million U / g to 200 million U / g, mannase with the enzyme activity of 10,000 U / g to 200 million U / g, cellulase with the enzyme activity of 1.2 million U / g to 25 million U / g, pectinase with the enzyme activity of 10,000 U / g to 25 million U / g, galactosidase with the enzyme activity of 10,000 U / g to 300 million U / g and phytase with the enzyme activity of 0.8 million U / g to 200 million U / g. According to the compound preparation provided by the invention, macromolecular substances such as starch in the dry coconut shreds can be degraded into micromolecular substances which are easy to digest, so that the digestion utilization rate is greatly increased; fat is degraded, fatty acid components such as palmitic acid, oleic acid and lauric acid in the coconut shreds are released, and the effects of reducing blood pressure, promoting digestion and the like are achieved; meanwhile, the generated active substances can improve the disease resistance of animals, improve the health state of organisms, improve the nutritional value of the dried shredded coconut and increase the variety of unconventional feed raw materials.

It is an object of the present invention to provide a highly safe protease (collagenase) that is useful for food or medical applications, such as collagen tripeptide production or cartilage degradation, the intended use thereof, and the like. According to the present invention, provided is an enzyme agent for collagen tripeptide production, comprising, as an active ingredient, a collagenase consisting of the amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence equivalent thereto.

The invention discloses a method for producing nicotinamide mononucleotide through double-enzyme'one-pot process' catalysis. According to the method, under the optimal conditions that EBY-NRK with the concentration of 25 mg / mL, EBY-PPK with the concentration of 10 mg / mL, NR with the concentration of 5 mM, ATP with the concentration of 1.5 mM, a phosphoric acid donor with the concentration of 15 mM and a Mg < 2 + >-containing substance with the concentration of 2 mM can be catalytically generated into 82.8% of NMN at the temperature of 30 DEG C and the pH value of 6.5, the ATP cost is reduced by 75%, and after a whole-cell enzyme preparation is repeatedly used for five times, the enzyme activity is still larger than 50%. The invention further develops a whole-cell catalyst displayed on the surface of the recombinant yeast, the problem of conversion inhibition caused by accumulation feedback of by-products in the NMN biosynthesis process is solved by coupling an ATP regeneration system, and the cost is effectively reduced while a good NMN product generation rate is obtained.

The invention relates to the field of pharmaceutical chemicals, in particular to a method for producing medicinal ethanol through fermentation of waste molasses. The method for producing the medicinal ethanol by fermenting the waste molasses comprises the following steps: adding a compound enzyme preparation into cane molasses, and treating to obtain enzyme treated molasses; inoculating wine-making active dry yeast for fermentation to obtain cooked mash; and carrying out crude distillation, dealdehyding and rectification on the prepared cooked mash to obtain the medicinal ethanol. The method for producing the medicinal ethanol through fermentation of the waste molasses is simple in technological process, the prepared fermentation liquor is high in alcoholic strength, the production efficiency is greatly improved, meanwhile, the use amount of corrosive strong acid is reduced, and the effects of saving energy, improving efficiency and reducing emission can be achieved for enterprises.

The present invention provides a liquid enzyme preparation containing a protein deamidase and having excellent activity stability, the liquid enzyme preparation comprising the protein deamidase together with another enzyme.　A liquid enzyme preparation comprising a protein deamidase and a protease is excellent with respect to the stability of the activity of the protein deamidase.

A method for preparing a high-purity yeast β-glucan, comprising the following steps: treating a yeast cell wall mixed liquid by adding amylase at a certain temperature, then adjusting the pH, and treating the mixed liquid by adding lipase at a certain temperature; adding an alkali and a nonionic surfactant, carrying out heating and thermal insulation treatment, and cooling to obtain an alkali-treated liquid; washing and separating the alkali-treated liquid, adding water to a precipitate, then adding an acid to adjust the pH to 3-5, carrying out heating and thermal insulation treatment to obtain an acid-treated liquid, and performing separation, washing, and drying on the acid-treated liquid to obtain a yeast β-glucan product; and subjecting the yeast β-glucan to a combined action of a mixed enzyme preparation and ultrasound to obtain an enzymatic hydrolysate, and performing separation treatment on the enzymatic hydrolysate to obtain an insoluble yeast β-glucan and a water-soluble yeast β-glucan, respectively. In the present invention, a high-purity yeast β-glucan product having a content of 90% or more and a water-soluble yeast β-glucan product with biological activity can be prepared, the preparation method is simple, large-scale production can be achieved, and the quality and additional value of the product can be improved.

The invention relates to the technical field of biological agents, and discloses a passivating enzyme soluble concentrate and a preparation process thereof.The soluble concentrate takes a specially-designed multifunctional deep eutectic solvent as a unique carrier, and the solvent has the multiple functions of stabilizing enzyme conformation, resisting oxidation, providing plant metabolism and increasing energy and the like. An active component is composite passivating enzyme powder which is subjected to vacuum freeze drying, and is compounded with a plant-derived phloem conduction enhancer in a synergetic manner. The preparation process adopts a core strategy of curing first and then dissolving, and the enzyme freeze-dried powder is dispersed in the non-aqueous solvent under low-temperature mild conditions. By constructing a water-free environment and adopting an activity protection process, the problems that a traditional water-based enzyme preparation is unstable in storage and the activity is easy to lose are fundamentally solved, and meanwhile, the bioavailability is remarkably improved by virtue of a targeted conduction component. The preparation has the remarkable advantages of ultra-long stability at normal temperature, high initial enzyme activity and strong targeting property.

The utility model discloses a stirring device for industrial enzyme preparation production, which relates to the technical field of enzyme preparation production and comprises a tank body, outer channel steel for reinforcement is sleeved at the bottom of the tank body, a cover plate is hermetically connected to the top of the tank body, and a feed pipe penetrates through the cover plate. A discharging pipe communicated with the interior of the tank body is arranged on one side of the tank body, and an observation window is formed in the side wall of the tank body. The conveying rod is driven by the output shaft of the motor to rotate in the conveying pipe, fermentation liquor at the bottom of the tank body can be conveyed to the upper portion along the conveying pipe, damage of shear force to zymoprotein molecules is reduced, the fermentation liquor flows all around under the guidance of the guide plate and the arc-shaped groove, the circulation speed of the fermentation liquor in the tank body is increased, and the fermentation efficiency is improved. The cells are in full contact with nutrient substances, it can be ensured that temperature distribution in fermentation liquor is more uniform, local overheating or supercooling is avoided, and the growth speed and enzyme production efficiency of the cells are improved.

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

A laundry composition and a preparation method therefor are provided, relating to the field of household necessities. The laundry composition includes a wrapping material, a functional material enveloped in the wrapping material, and an adhesive for bonding the wrapping material; the wrapping material is a film-forming material with laundry function; the functional material is selected from one or more of a softening particle, a fragrant particle, an active oxygen particle, and an enzyme preparation. By enveloping the functional material in the wrapping material with a detergent function, the laundry composition has integrated functions of cleaning, decontamination, pliancy, and fragrance-retaining. Furthermore, since the granular softening particle, a granular fragrant particle, a granular active oxygen particle, and a granular enzyme preparation are not directly added in the wrapping material, even if the wrapping material is formed by high-temperature drying, the functional materials will not fail.

SOLUTION: A laundry composition comprises an encapsulation material, a functional component encapsulated within the encapsulation material, and a binder used for bonding the encapsulation material, wherein the encapsulation material is a film-forming material having a washing function, and the functional component comprises one or more of softening particles, fragrance-retaining particles, active oxygen particles, and an enzyme preparation.EFFECT: By encapsulating the functional component with the encapsulation material having the washing function, the laundry composition can simultaneously achieve a cleaning and stain removal function, a softening function, and a fragrance-retaining function. In addition, since the softening particles, the fragrance-retaining particles, the active oxygen particles, and the enzyme preparation in the functional component are not directly added to the encapsulation material, it is possible to prevent the functional component from losing effectiveness when the encapsulation material is subjected to high-temperature press-forming, thereby solving a problem that effects are poor or costs are high when functional components are directly added to conventional laundry sheets.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of enzyme engineering and biology, and discloses an astragaloside beta-xylosidase mutant as well as a coding gene, a recombinant vector, a recombinant strain, an enzyme preparation and application thereof. The amino acid sequence of the mutant is mutated from at least one of the 23rd site, the 699th site and the 712th site of the amino acid sequence as shown in SEQ ID NO.1. The astragaloside beta-xylosidase mutant has the advantages that the enzyme catalytic activity and the hydrolysis conversion rate are obviously improved, so that the production efficiency of preparing cycloastragenol-6-O-glucoside from astragaloside through enzyme catalysis is obviously improved; good industrialization potential is realized.

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The present invention provides a feed enzyme preparation activity detection instrument, and particularly relates to the technical field of detection equipment, the feed enzyme preparation activity detection instrument comprises a detection box and a detection assembly arranged in the detection box, one side of the top surface of the detection box is provided with a display and a controller, and the center of the top surface of the detection box is provided with a placement port. The detection assembly comprises a transmitting device and a receiving device which are arranged on the two sides of the interior of the detection box respectively, and two sealing plates are arranged between the transmitting device and the receiving device, so that the cuvette can be sealed after the cuvette is placed by a detector and the sealing cover is closed; and then the transmitting device and the receiving device are driven to approach to each other for detection, so that unnecessary pollution on the surfaces of the transmitting device and the receiving device due to accidental contact between the detecting personnel and the transmitting device and the receiving device in the process of manually placing the cuvette is avoided, and the detection effect of the transmitting device and the receiving device during detection operation is ensured.

The invention provides an enzyme protective agent for a continuous glucose sensor and the continuous glucose sensor, the enzyme protective agent comprises the following components: poloxamer and a zwitterionic polymer, and the mass percent concentration of the poloxamer is 0.1%-2%, and the mass percent concentration of the zwitterionic polymer is 0.5%-5%. By adopting the technical scheme provided by the invention, the poloxamer and the zwitterionic polymer are introduced, so that an enzyme layer coating process can be optimized, a coffee ring effect can be inhibited, and the consistency and monitoring accuracy of the sensor can be improved; and on the premise of keeping the signal transmission performance of the sensor unchanged, the activity and long-term stability of the biological enzyme preparation in the production, storage, transportation and use processes are remarkably improved.

The invention discloses a preparation method of an enzyme preparation microcapsule with an intestinal tract slow release function. According to the method, coating wall materials in a specific proportion are adopted, the coating wall materials comprise 10-12 parts of Arabic gum, 4-8 parts of gelatin, 3-6 parts of whey protein, 8-16 parts of beta-cyclodextrin and 5-10 parts of trehalose, the coating wall materials are mixed with 10-15 parts of an enzyme agent, and the enzyme preparation microcapsule is prepared through a spray drying technology. The enzyme preparation microcapsule has excellent thermal stability and gastric juice tolerance, can maintain more than 90% of enzyme activity under the condition of 90 DEG C, and still maintains more than 65% of enzyme activity after being treated in simulated gastric juice for 4 hours. In addition, the enzyme preparation microcapsule has an intestinal directional slow-release function, the enzyme activity is quickly released after entering the intestinal tract for 15 minutes, and the peak value is reached after the enzyme preparation microcapsule enters the intestinal tract for 60 minutes. The method effectively improves the stability and bioavailability of the enzyme preparation in feed processing and animal digestive tract environment, and has good application prospect and market value.

The present invention discloses a method for preparing uric acid-lowering diglyceride by enzymatic hydrolysis, as well as its product and application, belonging to the technical field of diglyceride production. The method comprises the following steps: (1) mixing vegetable oil, glycerol, water, and an enzyme preparation to react to obtain a reaction solution; (2) allowing the reaction solution to stand for stratification and collecting the supernatant; (3) distilling and separating the supernatant, decolorizing, and deodorizing the supernatant to obtain diglyceride; the enzyme preparation in step (1) comprises lipase, esterase, and phospholipase C in a weight ratio of 5-10:0.1-0.3:1. Compared with the existing technology, the method of the present invention is simple and low-cost, and the prepared diglyceride has greater advantages in lowering uric acid.

The invention discloses an efficient deinking compound bio-enzyme preparation and application thereof, and belongs to the field of pulping and papermaking. The xylanase and the pectinase are adopted, xylan and pectic substances of paper fibers are destroyed through the synergistic effect of the xylanase and the pectinase, a fiber-ink binding area in paper pulp is hydrolyzed, and removal of ink particles is promoted; one or more of amylase, lipase and cellulase are also adopted, so that the degradation and removal effects of the enzymes on the ink are ensured, and the fiber strength is also improved; the enzyme stabilizer is added, so that the stability of the biological enzyme in the liquid preparation is ensured, and the efficient utilization rate of the deinking agent is realized; and the combination and the dosage ratio of the compound biological enzyme preparation are optimized, so that the deinking effect of the biological enzyme is remarkably improved. The method can improve the problem that the existing biological enzyme method deinking cannot obviously improve the physical properties of the paper, and has the advantages of environmental protection, low pollution and the like compared with a chemical method.

The invention discloses a neutral phytase mutant as well as a gene and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems that the existing neutral phytase is poor in thermal stability, poor in acidic environment stability, narrow in pH application range, low in low-temperature catalytic efficiency and the like. According to the technical scheme, the phytase preparation PHY06 with high stability and the gene and application of the phytase preparation PHY06 are provided. The method comprises the following steps: carrying out T329G, D333V, T353F and A364F mutation on a PHY03 sequence of which the amino acid sequence is shown as SEQ ID NO.1, so as to obtain a mutant PHY06;