641 results about "Enzyme agent" patented technology

The invention relates to an intelligent regulation and control method and system for fermentation parameters, a terminal and a medium, and belongs to the technical field of enzyme preparation fermentation. The intelligent regulation and control method for the fermentation parameters comprises the following steps: determining a current fermentation stage of a fermentation process; determining a dissolved oxygen target value and a pH target value according to the fermentation stage; acquiring monitoring data, wherein the monitoring data comprises a dissolved oxygen actual value, a dissolved oxygen change rate, a pH actual value, a pH change rate, a thallus concentration and a fermentation volume; if the change rate of the dissolved oxygen is a negative value, predicting a pH predicted value in future n minutes according to the actual value of the dissolved oxygen and the thallus concentration; judging whether an error value between the pH predicted value and the pH target value is not greater than an error threshold value or not; if yes, parameter intervention is carried out, and parameters comprise the ventilation capacity, the stirring speed and the alkali supplementing amount. The method has the beneficial effects that the fermentation parameters can be precisely regulated and controlled, so that the quality of a fermentation product is precisely improved.

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention relates to an automatic temperature control type concentration device for an industrial biological enzyme preparation, in particular to the technical field of enzyme preparation purification devices. The technical problem that a heating plate cannot conduct targeted heating on the position where enzyme-containing liquid is located is solved. Comprising a support, a heating shell is slidably connected to the interior of the support, a liquid storage tank is fixedly connected to the interior of the heating shell, a hot air pump is installed on the support, the hot air pump is fixedly connected with a first air conveying pipe, the first air conveying pipe is fixedly connected and communicated with the heating shell, and a sealing ring is slidably connected to the interior of the heating shell in a sealed mode. The sealing ring is connected with the outer side of the liquid storage tank in a sealed and sliding mode. The position of the sealing ring is changed along with the change of the liquid level of the stock solution in the liquid storage tank, so that the volume of hot air stored between the heating shell and the liquid storage tank is changed, the heating position of the liquid storage tank is further changed, the volume of the stock solution in the liquid storage tank is heated, and waste of the hot air is avoided.

The invention relates to the field of enzyme preparations, and discloses an enzyme preparation for catalytic degradation of bioplastics and a preparation method thereof, the enzyme preparation for catalytic degradation of bioplastics comprises the following components by weight: 25-50 parts of an enzyme, 1-8 parts of a catalytic additive, 2-10 parts of a stable dispersant, and 30-50 parts of a buffer solution; the catalytic additive is prepared by using calcium bentonite as a carrier to load nano titanium dioxide; the stabilizing dispersant is prepared by introducing polyethylene glycol monoallyl ether into a sodium lignin sulfonate structure by utilizing an initiator through an aqueous solution polymerization method, and by adding the catalytic additive and the stabilizing dispersant, the enzyme preparation is endowed with good degradation efficiency on bio-based plastics and the stability of an enzyme preparation system is improved.

The invention belongs to the technical field of biology, and discloses a halohydrin dehalogenase mutant, a coding gene, a recombinant vector, a recombinant strain, an enzyme preparation and application of the halohydrin dehalogenase mutant, the coding gene, the recombinant vector, the recombinant strain and the enzyme preparation in synthesis of chiral (S)-5-phenoxymethyl-2-oxazolidinone compounds. The halohydrin dehalogenase mutant is obtained by performing combined mutation on an enzyme with an amino acid sequence as shown in SEQ ID NO.1 at the 15th site, the 137th site and the 179th site. The mutant can obviously improve the ee value of a chiral (S)-5-phenoxymethyl-2-oxazolidinone compound synthesized by catalyzing phenyl glycidyl ether, the purity of catalytic synthesis and resolution is higher, and industrial production is facilitated.

It is an object of the present invention to provide a highly safe protease (collagenase) that is useful for food or medical applications, such as collagen tripeptide production or cartilage degradation, the intended use thereof, and the like. According to the present invention, provided is an enzyme agent for collagen tripeptide production, comprising, as an active ingredient, a collagenase consisting of the amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence equivalent thereto.

A method for preparing a high-purity yeast β-glucan, comprising the following steps: treating a yeast cell wall mixed liquid by adding amylase at a certain temperature, then adjusting the pH, and treating the mixed liquid by adding lipase at a certain temperature; adding an alkali and a nonionic surfactant, carrying out heating and thermal insulation treatment, and cooling to obtain an alkali-treated liquid; washing and separating the alkali-treated liquid, adding water to a precipitate, then adding an acid to adjust the pH to 3-5, carrying out heating and thermal insulation treatment to obtain an acid-treated liquid, and performing separation, washing, and drying on the acid-treated liquid to obtain a yeast β-glucan product; and subjecting the yeast β-glucan to a combined action of a mixed enzyme preparation and ultrasound to obtain an enzymatic hydrolysate, and performing separation treatment on the enzymatic hydrolysate to obtain an insoluble yeast β-glucan and a water-soluble yeast β-glucan, respectively. In the present invention, a high-purity yeast β-glucan product having a content of 90% or more and a water-soluble yeast β-glucan product with biological activity can be prepared, the preparation method is simple, large-scale production can be achieved, and the quality and additional value of the product can be improved.

The invention relates to the technical field of biological agents, and discloses a passivating enzyme soluble concentrate and a preparation process thereof.The soluble concentrate takes a specially-designed multifunctional deep eutectic solvent as a unique carrier, and the solvent has the multiple functions of stabilizing enzyme conformation, resisting oxidation, providing plant metabolism and increasing energy and the like. An active component is composite passivating enzyme powder which is subjected to vacuum freeze drying, and is compounded with a plant-derived phloem conduction enhancer in a synergetic manner. The preparation process adopts a core strategy of curing first and then dissolving, and the enzyme freeze-dried powder is dispersed in the non-aqueous solvent under low-temperature mild conditions. By constructing a water-free environment and adopting an activity protection process, the problems that a traditional water-based enzyme preparation is unstable in storage and the activity is easy to lose are fundamentally solved, and meanwhile, the bioavailability is remarkably improved by virtue of a targeted conduction component. The preparation has the remarkable advantages of ultra-long stability at normal temperature, high initial enzyme activity and strong targeting property.

The utility model discloses a stirring device for industrial enzyme preparation production, which relates to the technical field of enzyme preparation production and comprises a tank body, outer channel steel for reinforcement is sleeved at the bottom of the tank body, a cover plate is hermetically connected to the top of the tank body, and a feed pipe penetrates through the cover plate. A discharging pipe communicated with the interior of the tank body is arranged on one side of the tank body, and an observation window is formed in the side wall of the tank body. The conveying rod is driven by the output shaft of the motor to rotate in the conveying pipe, fermentation liquor at the bottom of the tank body can be conveyed to the upper portion along the conveying pipe, damage of shear force to zymoprotein molecules is reduced, the fermentation liquor flows all around under the guidance of the guide plate and the arc-shaped groove, the circulation speed of the fermentation liquor in the tank body is increased, and the fermentation efficiency is improved. The cells are in full contact with nutrient substances, it can be ensured that temperature distribution in fermentation liquor is more uniform, local overheating or supercooling is avoided, and the growth speed and enzyme production efficiency of the cells are improved.

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

A laundry composition and a preparation method therefor are provided, relating to the field of household necessities. The laundry composition includes a wrapping material, a functional material enveloped in the wrapping material, and an adhesive for bonding the wrapping material; the wrapping material is a film-forming material with laundry function; the functional material is selected from one or more of a softening particle, a fragrant particle, an active oxygen particle, and an enzyme preparation. By enveloping the functional material in the wrapping material with a detergent function, the laundry composition has integrated functions of cleaning, decontamination, pliancy, and fragrance-retaining. Furthermore, since the granular softening particle, a granular fragrant particle, a granular active oxygen particle, and a granular enzyme preparation are not directly added in the wrapping material, even if the wrapping material is formed by high-temperature drying, the functional materials will not fail.

SOLUTION: A laundry composition comprises an encapsulation material, a functional component encapsulated within the encapsulation material, and a binder used for bonding the encapsulation material, wherein the encapsulation material is a film-forming material having a washing function, and the functional component comprises one or more of softening particles, fragrance-retaining particles, active oxygen particles, and an enzyme preparation.EFFECT: By encapsulating the functional component with the encapsulation material having the washing function, the laundry composition can simultaneously achieve a cleaning and stain removal function, a softening function, and a fragrance-retaining function. In addition, since the softening particles, the fragrance-retaining particles, the active oxygen particles, and the enzyme preparation in the functional component are not directly added to the encapsulation material, it is possible to prevent the functional component from losing effectiveness when the encapsulation material is subjected to high-temperature press-forming, thereby solving a problem that effects are poor or costs are high when functional components are directly added to conventional laundry sheets.SELECTED DRAWING: Figure 1

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The present invention provides a feed enzyme preparation activity detection instrument, and particularly relates to the technical field of detection equipment, the feed enzyme preparation activity detection instrument comprises a detection box and a detection assembly arranged in the detection box, one side of the top surface of the detection box is provided with a display and a controller, and the center of the top surface of the detection box is provided with a placement port. The detection assembly comprises a transmitting device and a receiving device which are arranged on the two sides of the interior of the detection box respectively, and two sealing plates are arranged between the transmitting device and the receiving device, so that the cuvette can be sealed after the cuvette is placed by a detector and the sealing cover is closed; and then the transmitting device and the receiving device are driven to approach to each other for detection, so that unnecessary pollution on the surfaces of the transmitting device and the receiving device due to accidental contact between the detecting personnel and the transmitting device and the receiving device in the process of manually placing the cuvette is avoided, and the detection effect of the transmitting device and the receiving device during detection operation is ensured.

The invention provides an enzyme protective agent for a continuous glucose sensor and the continuous glucose sensor, the enzyme protective agent comprises the following components: poloxamer and a zwitterionic polymer, and the mass percent concentration of the poloxamer is 0.1%-2%, and the mass percent concentration of the zwitterionic polymer is 0.5%-5%. By adopting the technical scheme provided by the invention, the poloxamer and the zwitterionic polymer are introduced, so that an enzyme layer coating process can be optimized, a coffee ring effect can be inhibited, and the consistency and monitoring accuracy of the sensor can be improved; and on the premise of keeping the signal transmission performance of the sensor unchanged, the activity and long-term stability of the biological enzyme preparation in the production, storage, transportation and use processes are remarkably improved.

The invention discloses a preparation method of an enzyme preparation microcapsule with an intestinal tract slow release function. According to the method, coating wall materials in a specific proportion are adopted, the coating wall materials comprise 10-12 parts of Arabic gum, 4-8 parts of gelatin, 3-6 parts of whey protein, 8-16 parts of beta-cyclodextrin and 5-10 parts of trehalose, the coating wall materials are mixed with 10-15 parts of an enzyme agent, and the enzyme preparation microcapsule is prepared through a spray drying technology. The enzyme preparation microcapsule has excellent thermal stability and gastric juice tolerance, can maintain more than 90% of enzyme activity under the condition of 90 DEG C, and still maintains more than 65% of enzyme activity after being treated in simulated gastric juice for 4 hours. In addition, the enzyme preparation microcapsule has an intestinal directional slow-release function, the enzyme activity is quickly released after entering the intestinal tract for 15 minutes, and the peak value is reached after the enzyme preparation microcapsule enters the intestinal tract for 60 minutes. The method effectively improves the stability and bioavailability of the enzyme preparation in feed processing and animal digestive tract environment, and has good application prospect and market value.

The present invention discloses a method for preparing uric acid-lowering diglyceride by enzymatic hydrolysis, as well as its product and application, belonging to the technical field of diglyceride production. The method comprises the following steps: (1) mixing vegetable oil, glycerol, water, and an enzyme preparation to react to obtain a reaction solution; (2) allowing the reaction solution to stand for stratification and collecting the supernatant; (3) distilling and separating the supernatant, decolorizing, and deodorizing the supernatant to obtain diglyceride; the enzyme preparation in step (1) comprises lipase, esterase, and phospholipase C in a weight ratio of 5-10:0.1-0.3:1. Compared with the existing technology, the method of the present invention is simple and low-cost, and the prepared diglyceride has greater advantages in lowering uric acid.

The invention discloses a neutral phytase mutant as well as a gene and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems that the existing neutral phytase is poor in thermal stability, poor in acidic environment stability, narrow in pH application range, low in low-temperature catalytic efficiency and the like. According to the technical scheme, the phytase preparation PHY06 with high stability and the gene and application of the phytase preparation PHY06 are provided. The method comprises the following steps: carrying out T329G, D333V, T353F and A364F mutation on a PHY03 sequence of which the amino acid sequence is shown as SEQ ID NO.1, so as to obtain a mutant PHY06;

The invention relates to the technical field of biological catalysis, and discloses an M88F mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88F: methionine of the 88th amino acid sequence of UGT76G1 is mutated into phenylalanine; and the substrate rebaudioside A (RA) can be efficiently and directionally converted into rebaudioside I (RI) with higher value. In an optimized reaction system, the conversion rate stably reaches 40%, and the catalytic efficiency is improved by more than 5-8 times compared with the common reference enzyme. The enzyme preparation has the characteristics of high catalytic activity, mild reaction conditions, simplicity and convenience in operation and the like, shows good stability and reproducibility in laboratory research and large-scale production, and has a wide industrial application prospect.

A method for preparing theasinensin A (TSA) by means of a low-temperature enzyme process. The method comprises using a plant polyphenol oxidase enzyme extract or polyphenol oxidase enzyme formulation as an enzyme source, using epigallocatechin gallate (EGCG) as a substrate, using as a reaction system a buffer solution having a certain pH value, and performing an enzymatic reaction at a low temperature and subsequent steps such as column chromatography, distillation and concentration, liquid phase preparation, and freeze drying, thereby synthesizing TSA in a directional enzymatic manner. The method achieves EGCG enzymatic oxidation at a low temperature and obtains dimeric TSA by means of a conversion reaction, laying a theoretical and practical basis for green, safe, efficient and large-scale industrial production and utilization of TSA.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a phosphorylcholine cytidyltransferase mutant and application thereof. On the basis of phosphorylcholine cytidyltransferase MsCCT001, any one or more of N194D, K91E, G98S, S148A and C156V are mutated respectively, a series of phosphorylcholine cytidyltransferase mutants are obtained through screening, and the obtained mutants have the advantages that in the reaction of catalyzing phosphorylcholine and cytidine triphosphate to generate citicoline, the yield of the obtained mutants is increased, and the yield of the obtained mutants is increased. The catalytic efficiency of the compound is 1.90-2.41 times that of initial phosphorylcholine cytidyltransferase, and a biological enzyme preparation with higher catalytic capacity is provided for preparing citicoline by a biological conversion method.

The invention discloses a method for simultaneously producing ergothioneine and an enzyme preparation by utilizing trichoderma reesei, and belongs to the technical field of biological engineering. By optimizing the culture medium, a culture medium formula capable of enabling the trichoderma reesei which has almost no ergothioneine production capacity originally to synthesize the ergothioneine is screened out, and the trichoderma reesei can simultaneously produce the ergothioneine and an enzyme preparation. By further increasing the number of ergothioneine gene clusters of the trichoderma reesei, under the condition of not introducing any exogenous gene, an industrial production optimized strain of the ergothioneine and the enzyme preparation conforming to food safety is constructed, the yield of the ergothioneine and the enzyme preparation can be remarkably improved, and the production cost of the ergothioneine and the enzyme preparation is reduced. Meanwhile, the problems of biological safety, transgenosis safety and the like are solved, and technical support is provided for industrial production of ergothioneine and enzyme preparations.

The invention discloses an enzyme preparation for catalytic synthesis of D-chiro-inositol and a preparation method of D-chiro-inositol, and belongs to the technical field of genetic engineering. The enzyme preparation for catalytic synthesis of D-chiral inositol has enzyme activity for catalyzing the reaction of muscle inositol and NADP < + > to generate D-chiral inositol, and comprises inositol dehydrogenase and keto isomerase, the amino acid sequence of the inositol dehydrogenase is shown as any one of SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and the amino acid sequence of the keto isomerase is shown as any one of SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8. The invention further discloses a preparation method of the enzyme preparation for catalytic synthesis of D-chiral inositol. The inositol dehydrogenase and the keto isomerase in the enzyme preparation are mutated on the basis of wild types, so that the enzyme activity of the inositol dehydrogenase and the keto isomerase is improved, and the conversion rate of D-chiral inositol is further improved.

The invention discloses a multi-enzyme preparation for industrial cell dissociation as well as a use method and application thereof, and belongs to the technical field of extraction of seed cells for industrial culture of meat. According to the invention, a multi-enzyme preparation composed of a component 1 and a component 2 is adopted; the component 1 comprises neutral protease, hyaluronidase and elastase; and the component 2 comprises matrix metalloproteinase, papain and plasmin. The invention also discloses a preparation method and application of the multienzyme preparation. The reasonable enzyme ratio and the optimized treatment conditions are beneficial to protecting the integrity of cell membranes, maintaining high cell activity and remarkably improving the dissociation efficiency and the cell recovery amount; the complex enzyme preparation is high in adaptability, can be used for various tissue types and dissociation requirements, and is convenient for standardized and large-scale production; in combination with automatic dissociation equipment, the compound enzyme preparation can realize efficient and stable industrial operation, and provides important technical support and wide application prospects for the fields of meat culture industry, tissue engineering, cell therapy and the like.

The invention provides a hair drug detection reagent and a detection device, and belongs to the technical field of drug detection, the reagent comprises a lysis solution and a specific antibody labeling detection reagent; the lysate comprises the following components: a surfactant, a chelating agent, a reducing agent, an enzyme preparation, a stabilizer, a preservative and a buffer solution. The device comprises the reagent. The components of the lysate have a synergistic effect, so that a hair sample can be quickly and effectively lysed, and drugs or metabolites thereof can be released.

The invention discloses an enzyme preparation for catalytic synthesis of scyllo-inositol and a preparation method of scyllo-inositol, and belongs to the technical field of genetic engineering. The enzyme preparation has enzyme activity of catalyzing myo-inositol reaction to generate scyllo-inositol and comprises inositol dehydrogenase and scyllo-inositol dehydrogenase, and the amino acid sequence of the inositol dehydrogenase is shown as any one of (1) the amino acid sequence shown as SEQ ID NO.3; (2) an amino acid sequence as shown in SEQ ID NO.4; (3) an amino acid sequence as shown in SEQ ID NO.5; (4) an amino acid sequence as shown in SEQ ID NO.6; the amino acid sequence of the scyllo-inositol dehydrogenase is as shown in SEQ ID NO. 7. The specific sites of the wild type inositol dehydrogenase and the wild type scyllo-inositol dehydrogenase are mutated respectively, after mutation, the enzyme activity of the inositol dehydrogenase is higher, and the applicable pH range of the scyllo-inositol dehydrogenase is wider.

The invention relates to the technical field of sludge treatment and recycling, in particular to a novel sludge conditioner for a novel filter press, which is prepared from the following components in percentage by weight: 15 to 30 percent of modified humate, 5 to 12 percent of compound enzyme preparation, 8 to 20 percent of zwitterionic polymeric flocculant, 20 to 40 percent of inorganic skeleton construct, 3 to 10 percent of oxidant and 10 to 25 percent of hydrophobic modified mineral particles. And the balance of pH regulator and deionized water. According to the novel sludge conditioner disclosed by the invention, by compounding modified humate and other core components, a synergistic action mechanism is constructed, so that the water content of a sludge filter cake is less than or equal to 50-60%, the weight of the filter cake is reduced by over 20%, the COD (Chemical Oxygen Demand) of filtrate is reduced by over 30%, the novel sludge conditioner is adaptive to various filter presses, the filter pressing period is shortened by over 15% through an optimized conditioning process, the productivity is improved, and the treatment cost and the environmental protection pressure are reduced.

The invention discloses an organic waste fermentation method, which comprises the following steps: raw material pretreatment, rapid strain screening and domestication, dynamic segmented temperature control fermentation, efficient oxygen supply system, by-product collection and resource utilization, and final product collection and detection. Therefore, the carbon-nitrogen ratio, the water content and the pH value are optimized through raw material pretreatment, an efficient oxygen supply system and a decomposition enzyme preparation are combined, decomposition of complex organic matter is accelerated, product stabilization is optimized, a fully-closed fermentation reactor and a gas collection system effectively reduce environmental pollution, meanwhile, by-products such as heat energy and percolate are subjected to resource utilization, and the environmental pollution is reduced. Through rapid degradation of complex organic matters and real-time environment control, the fermentation period is obviously shortened to 10-14 days; by optimizing the microbial activity and the synergistic effect of the enzyme preparation, the problem that the microbial activity is limited in the prior art is solved; through resource utilization of by-products and full-closed design, pollution and resource waste are reduced, and the fermentation efficiency and the environmental protection performance are comprehensively improved.