314 results about "Enzyme agent" patented technology

A laundry composition and a preparation method therefor are provided, relating to the field of household necessities. The laundry composition includes a wrapping material, a functional material enveloped in the wrapping material, and an adhesive for bonding the wrapping material; the wrapping material is a film-forming material with laundry function; the functional material is selected from one or more of a softening particle, a fragrant particle, an active oxygen particle, and an enzyme preparation. By enveloping the functional material in the wrapping material with a detergent function, the laundry composition has integrated functions of cleaning, decontamination, pliancy, and fragrance-retaining. Furthermore, since the granular softening particle, a granular fragrant particle, a granular active oxygen particle, and a granular enzyme preparation are not directly added in the wrapping material, even if the wrapping material is formed by high-temperature drying, the functional materials will not fail.

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The invention relates to the technical field of biological catalysis, and discloses an M88F mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88F: methionine of the 88th amino acid sequence of UGT76G1 is mutated into phenylalanine; and the substrate rebaudioside A (RA) can be efficiently and directionally converted into rebaudioside I (RI) with higher value. In an optimized reaction system, the conversion rate stably reaches 40%, and the catalytic efficiency is improved by more than 5-8 times compared with the common reference enzyme. The enzyme preparation has the characteristics of high catalytic activity, mild reaction conditions, simplicity and convenience in operation and the like, shows good stability and reproducibility in laboratory research and large-scale production, and has a wide industrial application prospect.

The invention relates to the technical field of sludge treatment and recycling, in particular to a novel sludge conditioner for a novel filter press, which is prepared from the following components in percentage by weight: 15 to 30 percent of modified humate, 5 to 12 percent of compound enzyme preparation, 8 to 20 percent of zwitterionic polymeric flocculant, 20 to 40 percent of inorganic skeleton construct, 3 to 10 percent of oxidant and 10 to 25 percent of hydrophobic modified mineral particles. And the balance of pH regulator and deionized water. According to the novel sludge conditioner disclosed by the invention, by compounding modified humate and other core components, a synergistic action mechanism is constructed, so that the water content of a sludge filter cake is less than or equal to 50-60%, the weight of the filter cake is reduced by over 20%, the COD (Chemical Oxygen Demand) of filtrate is reduced by over 30%, the novel sludge conditioner is adaptive to various filter presses, the filter pressing period is shortened by over 15% through an optimized conditioning process, the productivity is improved, and the treatment cost and the environmental protection pressure are reduced.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The utility model discloses an efficient precipitation separation device for an enzyme preparation, and relates to the technical field of enzyme preparation processing. An organic solvent such as acetone or ethyl alcohol and an enzyme preparation are injected into the medicine box through the feeding pipe, after the driving machine is started, the driving wheel on the outer side of the driving machine can conduct circular motion around a circle, and therefore the limiting plate and the movable column can conduct circular motion in the vertical direction; furthermore, the medicine box can do reciprocating motion in the box body in the vertical direction, precipitation of the enzyme preparation can be accelerated under the cooperation of the organic solvent, and the subsequent effect is guaranteed; after the motor is started, the separation barrel can be driven to do circular motion, so that liquid medicament in the separation barrel can be centrifuged, the purpose of efficient separation can be achieved, and when the separation barrel rotates, the limiting seat synchronously does circular motion on the inner bottom wall of the box body, so that effective supporting of the separation barrel can be achieved, and inclination or shaking is avoided; and the actual use effect can be ensured.

The invention relates to the field of enzyme engineering, and provides a BhrPETase variant capable of improving stability through site-specific replacement under the background of Bhr6M (SEQ ID NO: 1) and application of the BhrPETase variant, and the replacement is M57I and / or S184H, preferably double mutation M57I / S184H. The variant shows remarkable thermal stability and storage stability under a unified measurement caliber: the condition t1 / 2 at 90 DEG C is greater than or equal to 400 minutes, the condition t1 / 2 at 70 DEG C is greater than or equal to 7 days, and the residual activity after 30 days of storage at 30 DEG C is greater than or equal to 90%. The invention also discloses a nucleic acid encoding the variant, a vector, a recombinant host, an enzyme preparation, and a method for depolymerizing PET and a copolymer thereof in a process temperature range of 70-90 DEG C. Compared with a control Bhr6M, the variant maintains more lasting activity in high-temperature and long-period operation, is suitable for PET substrates with different forms and crystallinity, reduces the enzyme supplementing frequency and improves the stability of a device.

The invention discloses an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. The enzyme-containing anti-inflammatory composition comprises an enzyme preparation, a hair follicle maintenance agent, a functional auxiliary agent and a carrier raw material. The core innovation lies in that a hair follicle targeted enzymolysis technology is adopted, and a specific enzyme modified by polyethylene glycol can efficiently dredge hair follicles; the anti-inflammatory and hair follicle repairing dual effects are achieved through anti-inflammatory-maintenance synergistic matching and adding sequence optimization; the damaged barrier is rapidly repaired by combining a composite barrier repairing agent and a gradient permeation process; enzyme activity and mildness are guaranteed by adopting a microcapsule embedding and low-temperature preparation technology. The composition can specifically solve the problems of hair follicle blockage, inflammation and unhairing, is suitable for pets of all ages and skin types, and is high in practicability.

A preparation method of N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate enzyme belongs to the field of medical intermediates, and comprises the following steps: step 1, preparing a buffer solution of a compound enzyme preparation; step 2, adding (2S)-2-benzyloxy-3-pentanone into a solvent A, and then adding a buffer solution of the compound enzyme preparation for reaction; 3, filtering, and carrying out an activation reaction on the filtrate; 4, filtering, removing the solvent, adding the obtained solid into a solvent B, and then adding formylhydrazine for reaction; 5, filtering, removing the solvent, adding the obtained solid into a solvent C, and then adding oxalic acid for reaction; and 6, drying the solid obtained by filtering to obtain the N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate. According to the method, the yield of the N '-((2S, 3S)-2-(benzyloxy) pent-3-yl) formylhydrazine oxalate can be increased, and the production cost is effectively reduced.

The invention discloses xylanase XynA00838, a truncated body of the xylanase XynA00838 for removing CBM1 and application of the xylanase XynA00838, and belongs to the technical field of microorganisms. The invention provides the XynA00838, the amino acid sequence of the truncation of the XynA00838 and the nucleotide sequence of the coding gene of the XynA00838, and provides characterization information, the XynA00838 and the truncation of the XynA00838 have obvious xylanase activity, and the specific enzyme activity of the truncation of the XynA00838 is obviously higher than that of wild type xylanase XynA00838 when six different plant polysaccharides are degraded, so that the XynA00838 and the truncation of the XynA00838 can be used for degrading various plant polysaccharides. When the truncated body degrades low-viscosity wheat arabinoxylan as a substrate, the specific enzyme activity reaches 4.46 + / -0.12 U / mg and is remarkably higher than that of wild type xylanase XynA00838. The CBM1 in the xylanase XynA00838 disclosed by the invention has a relatively good maintaining effect on the temperature stability of an enzyme catalysis module of the xylanase XynA00838. The truncated body XynA00838 delta CBM1 of the xylanase XynA00838 without the CBM1 has high enzyme activity and pH stability, so that the truncated body XynA00838 delta CBM1 has relatively great development and application potential, and a basic theoretical basis is provided for preparing a commercial feed enzyme preparation.

The invention relates to the technical field of preservation, in particular to a biological enzyme preparation preservation device.The biological enzyme preparation preservation device comprises a test tube, a sealing plug, a clamping jaw, a probe rod, a tank body and a heat preservation tape. Through the three-section tank body, a liquid enzyme preparation can be conveniently and rapidly taken and placed, an appropriate preservation environment can be provided for the liquid enzyme in cooperation with an internal temperature adjusting mode, and the preservation effect is good. Negative effects caused by external illumination and temperature are reduced, and the optimal activity is ensured; by means of the ball cage immersed in the liquid enzyme reagent, liquid enzyme can be promoted to flow below the liquid level, while bubbles are avoided, enzyme dry powder in a wall-hanging or precipitation state can be scoured and scattered through the acceleration effect of the spiral belt on the liquid enzyme preparation, and the dissolution rate of the enzyme dry powder can be further increased in cooperation with conical path swinging of the test tube; and the quality of the liquid enzyme is improved.

The invention discloses a pH response type endoscope biofilm cleaning agent based on polyether block phenylboronic acid ester and a preparation method of the pH response type endoscope biofilm cleaning agent, and the pH response type endoscope biofilm cleaning agent is prepared from the following components in percentage by mass: 0.05-0.5% of pH response type polyether block phenylboronic acid ester, 5-14% of a compound enzyme preparation, 0.5-2.0% of a sterilizing agent, 1.5-4.0% of a buffering agent, 0.8-2.5% of a chelating agent, 0.3-1.2% of a wetting agent, 3-10% of an enzyme stabilizer and the balance of water. And the balance of deionized water. A pH response type dynamic material is created for the first time, and intelligent switching of a single preparation between a storage state (pH 8.0-8.8) and a use state (pH 9.2-9.6) is achieved. While the long-term stability of the enzyme is guaranteed, the alkaline cleaning effect and instantaneous foam collapse are accurately triggered, and the long-term puzzling problems of the surfactant addition amount and the foam level are solved.

The utility model relates to the technical field of medical treatment and health, in particular to a stirring device for industrial enzyme preparation production, which comprises a production cylinder, a stirring device and a stirring device, the stirring mechanism is arranged in the production cylinder, and the stirring mechanism comprises a stirring assembly and a mounting mechanism; according to the industrial enzyme production device, production raw materials of industrial enzyme can be stirred through the stirring assembly, after the industrial enzyme at the production position is discharged, the cleaning mechanisms can be installed on the stirring assembly, water is added into the production barrel, and the industrial enzyme can be cleaned through the stirring assembly. The enzyme liquid matrix adhered to the inner wall of the production cylinder can be cleaned through the cleaning mechanism and taken out, the upper enzyme liquid matrix can be cleaned, and cross contamination caused by the residual enzyme liquid matrix to enzyme-producing strains in the next batch is avoided.

The invention relates to a preparation method of a lucid ganoderma and flax cake concentrated solution and a product. The preparation method comprises the following steps: S1, pretreating raw materials; s2, synergistic extraction: adding a compound enzyme preparation into the raw materials pretreated in the step S1, adjusting the pH value of the system to 4.5-5.5, and carrying out enzymolysis at 50-55 DEG C for 60-90 minutes, wherein the enzyme addition amount is 0.3%-0.5% of the mass of the mixed powder; raising the temperature of the system to 85-90 DEG C, carrying out constant-temperature extraction for 90-120 minutes, stirring once every 20 minutes during the extraction, and filtering by adopting a plate-and-frame filter after the extraction is finished, so as to obtain a polysaccharide extracting solution; s3, compounding and blending: respectively adding 5% of concentrated pear juice and 0.1% of concentrated lemon juice according to the mass percent of the polysaccharide extracting solution, uniformly stirring, adding deionized water to adjust the polysaccharide concentration to 10-15 mg / mL, and adjusting the pH value of the system to 4.5-5.0; and S4, sterilizing and filling: filtering the blended oral liquid stock solution through a 0.22 mu m microporous filter membrane, carrying out ultrahigh-temperature instantaneous sterilization, quickly cooling to 30 DEG C or below after sterilization, filling into a sterile oral liquid bottle in a sterile environment, sealing, and labeling to obtain the product.

The present application belongs to the technical field of cleaning supplies, and particularly relates to a high-efficiency blood-stain removing powder and a preparation method thereof. The high-efficiency blood-stain removing powder provided by the present application comprises the following raw materials and mass fractions: 45.0-65.0 parts of sodium percarbonate, 12.0-18.0 parts of an auxiliary agent, 11.0-18.0 parts of an auxiliary cleaning agent, 4.0-7.0 parts of anhydrous sodium metasilicate, 1.0-3.0 parts of a non-ionic surfactant, 1.5-2.5 parts of an enzyme preparation, 0.2-1.0 parts of an antibacterial agent, 1.0-3.0 parts of a thickening agent, and 0.1-0.5 parts of a fragrance; the auxiliary cleaning agent is composed of sodium carbonate and sodium alpha-alkenyl sulfonate at a mass ratio of 8.0-12.0:3.0-6.0. When the high-efficiency blood-stain removing powder of the present application is used, no complex operation is needed, and blood stains can be easily removed through simple soaking, without laborious scrubbing, so that the effect of 'quick cleaning and complete stain removal' is truly achieved.

The invention relates to the technical field of biological waste treatment, in particular to a preparation method and application of a silkworm sand feed additive. The invention provides a technical scheme for preparing a silkworm and mulberry sand feed additive rich in acid protease, pectinase and cellulase by taking silkworm sand as a core matrix and performing directional pretreatment, adaptive strain fermentation and standardized post-treatment. According to the invention, silkworm sand-corncob powder is used as a composite substrate, and the silkworm sand and the corncob powder complement each other in function, so that the problems of unbalanced nutrition and poor air permeability of a single substrate are solved. Specific aspergillus niger is used for segmented solid-state fermentation, parameters are set in a differentiated mode, and full-amount utilization of the silkworm sand is achieved; the product quality and enzyme activity are improved by optimizing the process, and the resource value is improved from the low-added-value field to the high-added-value enzyme preparation industry.

The application belongs to the technical field of enzyme preparation processing, and specifically provides a preparation method of a gastric-stable enzyme preparation, which comprises the following steps: (1) placing enzyme liquid in a stirrer, adding stabilizers and carriers into the enzyme liquid, uniformly stirring, then adding emulsifiers and emulsion stabilizers, uniformly stirring again, obtaining enzyme mixture, and heating and keeping warm; (2) heating hot-melt type packaging materials to completely melt into liquid state, and keeping the packaging materials in liquid state; (3) starting the stirrer, slowly pouring the liquid hot-melt type packaging materials into the stirrer while stirring, and obtaining a material liquid; (4) spray drying the material liquid, and obtaining the gastric-stable enzyme preparation. The preparation method provided by the application effectively avoids the destruction of the prepared enzyme preparation by pepsin after the enzyme preparation enters the stomach of an animal, improves the enzyme activity retention rate of the enzyme preparation product after passing through the stomach, and further improves the stability of the enzyme preparation in the storage process, especially the storage stability in a humid environment.

The application discloses a marine-derived cholesterol oxidase and a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the marine-derived cholesterol oxidase (SkChOx) is shown in SEQ ID NO. 1 or 3, the specific enzyme activity of the marine-derived cholesterol oxidase can reach 31.9 U / mg, the marine-derived cholesterol oxidase has excellent anti-reverse performance, maintains high stability in the pH range of 5.0-10.0, has good temperature tolerance, organic solvent tolerance and metal ion tolerance, and exhibits unique salt activation effect and salt tolerance. The enzyme is compounded with dithiothreitol (DTT) and nonylphenol polyoxyethylene ether (NP-40) to form a liquid enzyme preparation, and after incubation at 37 DEG C for 7 days under accelerated conditions, the catalytic activity of the liquid enzyme preparation still remains about 87%, the liquid enzyme preparation has excellent long-acting storage stability, and has wide application prospects in the fields of clinical diagnosis, food physical and chemical inspection, biosensor development and industrial preparation of steroid drugs.

The invention discloses a fabric sand washing process and a sand-washed fabric, and relates to the technical field of fabric treatment.The fabric sand washing process comprises the following steps that S1, a wet fabric is placed in a biological enzyme treatment tank, a biological enzyme preparation, a penetrating agent and a buffering agent are added, and oscillation treatment is conducted; s2, adding a nano silicon-based grinding material into the biological enzyme treatment tank, and starting ultrasonic waves to carry out wear treatment; s3, rinsing the treated fabric, and filtering the wastewater in the biological enzyme treatment tank. According to the invention, by introducing bio-enzyme pretreatment in combination with a synergistic mechanism of the nano silicon-based abrasive and ultrasonic waves, strong mechanical friction of traditional pumice on the light and thin fabric is avoided, and the risk of damage of the fabric in the treatment process is reduced while the sand washing effect is ensured.

The invention belongs to the technical field of biology, relates to a zearalenone degrading enzyme, and particularly relates to a bacillus subtilis source zearalenone degrading enzyme, an engineering strain thereof and an enzyme preparation prepared from the bacillus subtilis source zearalenone degrading enzyme. Compared with the known zearalenone degrading enzyme, the zearalenone degrading enzyme not only has obvious structure and sequence differences, but also shows an excellent degrading effect at low concentration. The lactone hydrolase derived from bacillus is found for the first time, and the biological resource basis of zearalenone degrading enzyme is expanded. The enzyme not only has significant difference from other degrading enzymes in structure, but also shows unique characteristics in degradation mechanism. The capability of efficiently degrading zearalenone shows that the bacillus subtilis has a wide application prospect in the fields of agriculture, food industry and the like. In addition, the invention also covers the structure of a degradation product generated by the zearalenone enzyme, and provides a new perspective for understanding a zearalenone degradation mechanism.

The invention discloses a colorless capsicum oleoresin microemulsion and a preparation method and application thereof, and belongs to the technical field of food processing.The preparation method of the colorless capsicum oleoresin microemulsion comprises the following steps that oil-soluble capsicum oleoresin and an emulsifier are heated, then water is added for mixing, and water-soluble capsicum oleoresin is obtained; adding an enzyme preparation into the water-soluble capsicum oleoresin, and carrying out ultrasonic enzymolysis reaction to obtain an enzymolysis solution; and carrying out enzyme inactivation treatment on the enzymolysis solution, and then centrifuging to remove precipitate to obtain supernate, namely the colorless capsicum oleoresin microemulsion. According to the method, colored capsanthin is degraded into colorless substances through mild enzymolysis reaction, and meanwhile, capsaicin molecules are prevented from being damaged. The colorless capsicum oleoresin microemulsion prepared by the invention has the advantages of high stability, improved fluidity and few impurities, and is beneficial to application in colorless or light-colored food.

The invention discloses a demulsifier based on enzyme-nano composite microspheres as well as a preparation method and a cascade application method thereof, and belongs to the technical field of sewage treatment. The invention aims to solve the technical problems that the existing demulsifier is poor in sewage degradation effect, poor in free enzyme stability, easy to inactivate and the like. According to the key point of the technical scheme, the demulsifier based on the enzyme-nano composite microspheres comprises the following components in percentage by dry weight of the composite microspheres: 30-60% of a polymer matrix, 10-40% of an enzyme preparation and 15-50% of a nano silicon-based material.

The present application relates to the field of shoe leather production, and particularly relates to a production process of fluorine-free dynamic waterproof suede shoe leather. First, the suede microfiber base leather is soaked in a composite biological enzyme preparation to remove surface impurities and open the fiber gap, and then is treated by helium-oxygen mixed gas normal pressure plasma to build micro-pits and active groups on the fiber surface. Subsequently, the base leather is sequentially soaked in waterproof agents containing various modified substances, and a two-stage gradient temperature and pressure padding process is adopted to realize the gradient distribution and crosslinking of the waterproof agent from inside to outside. Finally, a three-stage temperature oscillation drying is adopted, the crosslinking reaction is triggered in the medium temperature section, the siloxane chain is induced to directional arrangement to form a stress buffer layer in the high temperature section combined with mechanical oscillation, and the finished product is obtained through low temperature polishing and setting. The process completely avoids fluorine-containing compounds, solves the problem of easy cracking of traditional waterproof leather in dynamic bending, and maintains the softness of suede. The process is suitable for high-end shoe materials, outdoor equipment and other fields.

The present invention relates to a method for preparing an enzyme blend, the method comprising the steps of: providing a fermentation-derived preparation of a first enzyme of interest; exposing the first enzyme of interest preparation to a temperature higher than the fermentation temperature; purifying the first enzyme from the fermentation-derived preparation; and adding at least one second enzyme of interest to the purified first enzyme; thereby obtaining an enzyme blend. Furthermore, the present invention relates to an enzyme blend obtainable or obtained by the method according to the present invention.

The present application belongs to the field of leather industry production, and particularly relates to a method for high absorption chrome tanning by using cross-linking enzyme preparation. The method comprises the following steps: water immersion, unhairing and liming, deliming, softening, enzyme-assisted tanning, and acid immersion tanning. The enzyme-assisted tanning is assisted by using cross-linking enzyme preparation. The cross-linking enzyme preparation is not used in combination with the hydrolytic enzyme in the softening step. The cross-linking enzyme comprises glutamine transaminase (TG enzyme). The comprehensive activity of the cross-linking enzyme is controlled to be 1-30 U / ml in the application process, and the activity of the TG enzyme is controlled to be 1-20 U / ml. The process in the present application meets the environmental protection requirements. Firstly, the tanning agent absorption rate in the tanning process is successfully improved, and the waste discharge amount in the tanning process is reduced. Secondly, the cleaner cross-linking enzyme preparation is successfully used to replace the chemical auxiliary agent to improve the tanning agent absorption rate. Thirdly, the process operation is simplified.

The invention relates to the technical field of bioengineering and environmental biology, and particularly discloses high-temperature-resistant polylactic acid depolymerases and application thereof in degradation of polylactic acid plastics. The nucleotide sequence of the coding gene of the high-temperature-resistant polylactic acid depolymerases B01 is as shown in SEQ ID NO: 1, and the amino acid sequence of the coding gene is as shown in SEQ ID NO: 2. The enzyme is derived from metagenome mining, the sequence similarity of the enzyme and the known PLA depolymerases PAM is only 34.45%, and the enzyme has remarkable novelty. According to the present invention, the secretory expression and the purification of the B01 are successfully achieved in the bacillus subtilis SCK6; an enzymatic property research shows that the optimal reaction temperature of the B01 is 65 DEG C, the half-life period at 60 DEG C is as long as 150 minutes, and the B01 shows excellent thermal stability; and the optimum pH value of the strain is 9.0. The invention provides a novel enzyme preparation with important application potential for high-temperature biological recovery treatment of PLA plastic waste.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The invention belongs to the technical field of enzyme engineering, and particularly relates to an immobilized enzyme preparation containing D-psicose-3-epimerase cells as well as a preparation method and application of the immobilized enzyme preparation. The preparation method comprises the following steps: carrying out fermentation culture on genetically engineered bacteria containing thermal-stability D-psicose-3-epimerase to obtain wet thalli, mixing the thalli with egg shells and a diatomite carrier, immobilizing the obtained mixture on the carrier through a cross-linking agent, and carrying out separation, drying and granulation to obtain the immobilized enzyme preparation. According to the invention, the affinity between the immobilized carrier and microorganisms is stronger; the prepared immobilized enzyme preparation is high in mechanical strength and stable in enzyme activity, is used for preparing D-psicose through D-fructose isomerization, and is high in conversion efficiency. Under the condition of 60 DEG C, after the batch reaction is repeated for 10 batches, the conversion rate is still greater than 28%; 700g / L fructose solution is used as a substrate, the reaction is continuously carried out for 31 days, the average conversion rate is 28% or above, and the method has relatively high industrial advantages.

A brightening and stain-removing laundry sheet and a preparation method therefor. The laundry sheet comprises: a film-forming agent, an anionic surfactant, a nonionic surfactant, a fluorescent brightening agent, an anti-fouling agent, an enzyme formulation, a plant extract, a chelating agent, and an auxiliary agent. The film-forming agent comprises a film-forming agent A and a film-forming agent B, the film-forming agent A being polyvinyl alcohol, and the film-forming agent B comprising one or more of polyvinylpyrrolidone and modified polyvinyl alcohol. The anionic surfactant comprises sodium alkyl sulfate, sodium fatty acid methyl ester sulfonate, and sodium α-alkenyl sulfonate; and the nonionic surfactant comprises polyglycerol ester of fatty acid, fatty alcohol-polyoxyethylene ether, fatty acid methyl ester ethoxylate, and alkyl polyglycoside. The laundry sheet has high solubility, achieves a remarkable brightening effect on fabrics while having stain-removing capability, and further possesses the advantages of good portability, convenient use, no solid matter remaining after use, etc.