The invention provides an RPA (
recombinase polymerase amplification) method, an PRA special primer and an RPA probe for detecting universal human adenovirus, and application of the PRA special primerand RPA probe in detection of the universal human adenovirus. The detection method, the special primer and the probe are designed based on a common
conserved sequence of type 3, type 7, type 11, type21 and type 55 human adenovirus hexon genes, and have the
oligonucleotide sequences shown as SEQ ID NO. 3, SEQ ID NO. 4 and SEQ ID NO. 5. According to the method, the novel isothermal amplification technology is applied to detection of the universal human adenovirus for the first time; the enzymatic reaction process of in-vivo
DNA replication is simulated, a
specific enzyme and
protein combination(
recombinase, single-stranded
binding protein and
DNA polymerase) is used for
DNA template amplification, amplification of a specific
nucleotide sequence can be realized at a constant temperature of37 DEG C, and amplification products can be visually judged through a lateral chromatographic test strip. The detection method has high sensitivity, and the
detection limit can reach 101 copies / reaction; the specificity is high, and no cross-reaction with other pathogens exists; the detection speed is high, and the method is simple and convenient to operate.