8 results about "Taq polymerase" patented technology

The invention provides a ready-to-use PCR tube preloaded with Taq polymerase and reaction components and stable at room temperature, and belongs to the field of molecular biology and nucleic acid diagnosis. The invention provides a ready-to-use PCR (polymerase chain reaction) tube which can be stored at room temperature and is pre-filled with a premix for carrying out polymerase chain reaction (PCR) amplification on all necessary components in advance. The kit is characterized in that each tube is pre-filled with locally prepared Taq polymerase, a PCR buffer solution, dNTPs, MgCl and target specific primers (only a customized PCR kit-ready-to-use PCR plus tube contains the primers), and all the components are dried and stored in a dry powder form, so that the shelf life can be prolonged, and the tedious steps of on-ice operation can be avoided. According to the method, the error rate in the PCR preparation process is reduced to the maximum extent, and the diagnosis and research work flow is simplified.

The invention discloses a preparation method and application of a Taq DNA polymerase monoclonal antibody based on structural domain analysis, which are specifically different from a traditional method using full-length Taq polymerase as an immunogen (the method generally cannot generate a functional blocking antibody due to a hidden epitope which is difficult to approach). According to the invention, by adopting a structure-oriented and domain-specific strategy, the exposure of epitopes related to active sites is remarkably enhanced, and a novel antigen Taq-P protein is developed. Based on the antigen, a high-specificity inhibitory monoclonal antibody is prepared, and the antibody is expected to become a core component of a high-performance hot start PCR reagent. According to the invention, a novel and generalizable mode is established for the development of a core diagnostic reagent, a key raw material is provided for gene detection and molecular diagnosis, and a valuable methodological framework is contributed to the development of propulsor engineering and biotechnology reagents.

This invention relates to a real-time fluorescent PCR solid detection reagent and its preparation method. The real-time fluorescent PCR solid detection reagent is prepared by freeze-drying a mixture of a protective system, a glycerol-free buffer solution and dNTPs premix, specific primers and probes, and glycerol-free Taq polymerase. The real-time fluorescent PCR solid detection reagent of this invention can maintain its original diagnostic efficacy and sensitivity for at least two years at room temperature, and can detect nucleic acid molecules at a minimum of two copies. This solves the problems of existing real-time PCR detection reagents requiring immediate preparation and strict cold chain technology during long-distance transportation, which significantly increases transportation and storage costs, leading to long transportation times and insufficient reagent inventory in some underdeveloped areas.

This disclosure relates to a Taq polymerase-conjugated antibody composition and a method for nucleic acid amplification.

This invention relates to the field of molecular biology and nucleic acid diagnostics: specifically, compositions and methods to facilitate convenient, error- proof, reliable, portable and cost-effective PCR reactions. This universal PCR kit is suitable for use in clinical diagnostics, pathogen detection, genetic screening, forensic testing, and research laboratories, particularly in resource-limited settings or wherever cold-chain maintenance is impractical or difficult to maintain. The present invention provides ready-to-use, storeable at room temperature PCR tubes including master mixes containing all essential components for polymerase chain reaction (PCR) amplification. Each tube is preloaded with an indigenously prepared Taq polymerase enzyme, PCR buffer, dNTPs, MgCl2, and target-specific primers (only in case of customized PCR kits) in a dried formulation for extended shelf-life. This invention aims to reduce cold-chain requirements, minimize handling and preparation errors, streamline diagnostic and research workflows. Conventional PCR workflows require multiple liquid reagents stored under refrigeration or freezing conditions. Preparing each reaction requires multiple and precise aliquoting which is time-consuming specially when dealing with large sample numbers and prone to errors and contamination due to multiple pipetting and repeated actions. While commercial lyophilized PCR kits exist, they typically rely on expensive imported enzymes, require lengthy procurement procedures, and several handling / rehydration steps involving extensive cold chain and difficult to maintain lab resources. The present invention has a locally produced Taq polymerase and a novel drying process that confers extended stability at ambient temperatures, eliminating the need for ultra-low temperature shipping and storage. The ready to use nature in single tube format, room temperature storage / shipment and availability at comparatively low cost makes this technology a viable product for commercialization with ideal solution for biology work having bright commercial potential.

This invention provides a method for detecting telomerase activity in plants of the genus *Catella*, belonging to the field of plant telomerase detection technology. Specifically, it includes the following steps: telomerase solution samples are added to a premixed solution, and DEPC-treated water is added to make up the volume. This premixed solution includes TRAP buffer, dNTP mixture, leader primer, reverse primer, and Taq polymerase. PCR amplification is then performed, with the following amplification program: PCR extension at 26 °C for 30 min; pre-denaturation at 95 °C for 2 min; 30 cycles of 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 60 s; 72 °C for 10 min; and storage at 4 °C. After amplification, the PCR amplification products are subjected to agarose gel electrophoresis, stained with SYBR Green I staining solution, and observed under a gel imaging system. This invention proposes a method for detecting telomerase activity in plants of the genus *Catella*, applicable to various plants in the genus, and the testing process is safe and reliable.

This disclosure relates to Taq polymerase binding antibody compositions and methods of use for nucleic acid amplification.

This disclosure relates to Taq polymerase binding antibody compositions and methods of use for nucleic acid amplification.