Western blot kit for detecting antibody of autoimmune disease and preparation method thereof

Abstract

The invention provides a western blot kit for detecting the antibody of autoimmune disease and a preparation method of the western blot kit, and relates to a western blot kit for detecting related antibodies of various autoimmune diseases, aiming at overcoming the technical defect that a western blot product is unavailable for testing and screening various autoimmune diseases in the prior art. The nitrocellulose membrane or the nylon membrane contains at least two parallel detection lines coated by at least two of ten natural antigens or recombinant antigens, i.e. dsDNA (deoxyribonucleic acid), Sm / RNP (ribonucleoprotein), CCP (critical compression pressure), SSA (sulfosalicylic acid), SSB (single-strand binding protein), GAD (glutamic acid decarboxylase), ICA (islet cell antibody), IA-2A (islet cell), TG (triglyceride) and AMA-M2 (anti-mitochondrial antibody), a high-concentration quality control band, a median-concentration quality control band and a low-concentration quality control band. The deficiency of the detection sensitivity and the specificity of the single autoantibody can be overcome, the operating complexity for independently detecting the related autoantibody of various diseases one by one can be overcome, and the detection efficiency and the result judging accuracy degree can be greatly improved.

Description

technical field [0001] The invention relates to an immunoblotting kit for detecting antibodies related to various autoimmune diseases, and also relates to a preparation method and a use method of the immunoblotting kit, belonging to the field of biomedicine. Background technique [0002] Autoimmune diseases (autoimmune diseases) refer to diseases caused by the body's immune response to self-antigens, resulting in damage to its own tissues. WHO (World Health Organization, World Health Organization) once pointed out that the number of patients with this disease accounts for 3~3% of the total population. According to authoritative statistics, the incidence of autoimmune diseases in my country is more than 60 million. Autoimmune diseases tend to occur in women and middle-aged and elderly people. They are characterized by slow onset, long course of disease and repeated attacks. The middle and late stages of the disease are very harmful to health, the quality of life of patients is...

AI Technical Summary

Problems solved by technology

At present, there are a few western blot products for the detection of antibodies related to a single autoimmune disease in the field of clinical testing at home and abroad, but there are no western blot products for the physical examination screening of multiple autoimmune diseases

The existing immunoblotting reagents basically use SDS-PAGE technology to transfer antigens to the blot membrane according to their molecular weights. During this process, the conformation of many antigenic determinants may be destroyed, and they cannot bind to or form specific antibodies in clinical samples. Non-specific binding, resulting in misjudgment of results

Moreover, the existing product quality control system is not perfect, and it is not convenient for the operator to quickly, intuitively and accurately judge the color rendering results

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