Kit for detecting 30 food allergen IgG antibodies and preparation method of kit
A food allergy and kit technology is applied in the field of biomedical in vitro diagnosis to achieve the effects of improving detection efficiency, high stability and high sensitivity
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[0058] The embodiment of the present invention provides a preparation method for detecting 30 food allergen IgG antibody kits, including a method for preparing a membrane strip, and the preparation method for the membrane strip includes the following steps:
[0059] S1, using coating buffer, broccoli, cabbage, carrots, cauliflower, celery, cucumber, eggplant, green peas, green peppers, lettuce, mushrooms, onions, parsley, variegated peas, potatoes, spinach, squash, green beans, Sweet potato, coriander, tomato, cabbage, barley (whole grain), buckwheat, malt, millet, oats, rice, wheat, corn, a total of 30 common food-specific allergens and anti-sheep IgG, human IgG diluted to the relative package Concentration, preparation of allergen coating solution; wherein, the allergen adopts lyophilized powder, and the coating buffer is dissolved in 30mmol / L tris buffer containing stabilizer, and the allergen The concentration of the original coating solution is 0.1mg / ml-1mg / ml;
[0060] ...
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