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Kit for detecting 30 food allergen IgG antibodies and preparation method of kit

A food allergy and kit technology is applied in the field of biomedical in vitro diagnosis to achieve the effects of improving detection efficiency, high stability and high sensitivity

Inactive Publication Date: 2017-05-10
SIDERUN BEIJING MEDICAL DIAGNOSTIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a test kit and preparation method for detecting 30 food allergen IgG antibodies, thereby solving the aforementioned problems existing in the prior art

Method used

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  • Kit for detecting 30 food allergen IgG antibodies and preparation method of kit
  • Kit for detecting 30 food allergen IgG antibodies and preparation method of kit
  • Kit for detecting 30 food allergen IgG antibodies and preparation method of kit

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Embodiment 2

[0058] The embodiment of the present invention provides a preparation method for detecting 30 food allergen IgG antibody kits, including a method for preparing a membrane strip, and the preparation method for the membrane strip includes the following steps:

[0059] S1, using coating buffer, broccoli, cabbage, carrots, cauliflower, celery, cucumber, eggplant, green peas, green peppers, lettuce, mushrooms, onions, parsley, variegated peas, potatoes, spinach, squash, green beans, Sweet potato, coriander, tomato, cabbage, barley (whole grain), buckwheat, malt, millet, oats, rice, wheat, corn, a total of 30 common food-specific allergens and anti-sheep IgG, human IgG diluted to the relative package Concentration, preparation of allergen coating solution; wherein, the allergen adopts lyophilized powder, and the coating buffer is dissolved in 30mmol / L tris buffer containing stabilizer, and the allergen The concentration of the original coating solution is 0.1mg / ml-1mg / ml;

[0060] ...

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Abstract

The invention discloses a kit for detecting 30 food allergen IgG antibodies and a preparation method of the kit and relates to the technical field of biomedicine in-vitro diagnostics. Line immunoassay is adopted, and totally 30 food specific IgG allergens such as broccoli, cabbage, carrot, cauliflower, celery, cucumber, eggplant, green pea, green pepper, lettuce, mushroom, onion, parsley, colorful pea, potato, spinach, pumpkin, green soybean, sweet potato, caraway, tomato, pakchoi, barley (all kernel), buckwheat, malt, millet, oat, rice, wheat and corn as well as a quality control line and a critical value line are coated on a membrane strip prepared on a nitrocellulose membrane in parallel, so that the kit is high in sensitivity (signal value) and high in stability. Moreover, due to a clinical sample and primary detection reaction, the IgG antibodies of the 30 food allergens in human serum can be detected, the complicated operation in the process of detecting the Elisa methodology kit antibodies one by one is avoided, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of biomedical in vitro diagnosis, in particular to a kit and a preparation method for detecting 30 food allergen IgG antibodies. Background technique [0002] At present, food allergens in clinical laboratory samples are generally detected by enzyme-linked immunosorbent assay (Elisa). [0003] This method is to coat allergens in 96 microwell plates, incubate and react clinical samples, and finally interpret the results with a microplate reader. This method can usually detect 14 food allergen IgG at one time, and one experiment can only The detection of a single indicator has low throughput and high detection cost, which has limitations in expanding the allergen detection project. Contents of the invention [0004] The purpose of the present invention is to provide a kit and preparation method for detecting 30 food allergen IgG antibodies, thereby solving the aforementioned problems in the prior art. [0...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/535
CPCG01N33/558G01N33/535G01N33/54306
Inventor 张逸南吴利娅孙冬娜刘立敏李高扬
Owner SIDERUN BEIJING MEDICAL DIAGNOSTIC PROD CO LTD
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