105 results about "Allergens food" patented technology

There is described a substrate to which is applied, coupled or cross-linked to at least one part of said substrate an allergen wherein said allergen is a protein derived from an extract selected from at least one of the following food groups: cereal, legume, cocoa bean, nut, fruit, vegetable, shellfish, fish, yeast, dairy product, egg and meat.

The present invention is directed to a method and apparatus that satisfies the need for a bioelectronic tongue for food allergy detection. The method of detecting concentration of food allergen incorporates antibodies into an electronic tongue to create a bioelectronic tongue. Additionally the method uses impedance, capacitance, and / or other related electrochemical methods for detecting analyte in complex media. Furthermore the method additionally includes methods to subtract out non-specific interactions. The method also subtracts non-specific interactions. The device / apparatus is a Bioelectronic Tongue for detecting allergen in diluted food samples. The device includes: a sensor array; an impedance or capacitance analyzer; a preprocessor; a feature extractor; a pattern recognizer; and an output device indicating an allergen concentration. In order to implement the method of detecting food allergens on a bioelectronic tongue a computer readable medium containing an executable program is used for performing the analysis of a food sample. The executable program performs the acts of: preprocessing data from an impedance analyzer; extracting a feature pattern; recognizing a pattern of features of data representing a concentration of food allergen contained is the food sample; and outputs allergen concentration data.

The invention discloses an ELISA detection kit of food allergen specificity IgG4 antibody and preparation method thereof, using enzyme target rod coated by purified food allergen protein. The kit comprises: enzyme target rod coated by food allergen protein, human IgG2 titer, enzymic-lableled antibody, TMB substrate developing liquid, sample diluent, concentrated cleaning solution and termination reaction liquid. The specificity IgG4 antibodies in sample serum are bonded with the corresponding antigens fixed on the micropore surface of the enzyme target rod and the enzymic-lableled antibodies identify and bond with the IgG4 to form antigen-IgG4-antibody-enzyme composite. The absorbency is measured by the development reaction between the enzyme and substrate and the specificity IgG4 antibody concentration in sample serum is measured by standard curve. The allergen specificity IgG4 antibody concentration in sample serum is used as biology index of exposure state of food allergen and for judging the patient immune deviation and immune tolerance and monitoring the immunotherapy effectiveness of special food selected by patients.

An internet based system for analyzing menu items offered by restaurants / food providers to identify menu items which are acceptable to person is disclosed. The system includes an online system having a server in communication with a database of food ingredients for menu items offered by a food provider. The system compares the food ingredients of menu items offered by food providers and compares it with the dietary and / or allergen information of one or more people to identify menu items offered by food provider that are acceptable to the each person.

A complete nutritional composition comprising Lactococcus strains or probiotic is provided for reducing the symptoms of allergies in different groups of patients such as allergies originating from food allergens in young children or infants and respiratory allergens in children, adults and household pets. Preferably the composition reduces symptoms of allergies (secondary prevention) while not significantly affecting sensitization (primary prevention). The composition comprises a probiotic of the genus Lactococcus.

The present invention provides allergen detection molecules and devices useful in on-site and rapid detection of allergens, including food allergens.

This document provides methods and materials related to detecting allergens (e.g., food allergens) and / or specific antibodies in individualized consumers that are specific to an allergen (e.g., a food allergen). For example, methods and materials for assessing a food sample for the presence or absence of food allergens are provided. In addition, methods and materials for assessing if a particular human will react with those exact food allergens given his / her antibody profile are provided.

The invention provides a chemiluminiscence diagnostic kit for sensitization allergens and a preparation method thereof. The diagnostic kit comprises a microporous plate coated with the sensitization allergens, a horseradish peroxidase-labeled anti-human IgE (immunoglobulin e) antibody, chemiluminiscence substrate solutions A and B, and a washing solution, wherein the sensitization allergens comprise one or more of an insect allergen, a pollen allergen, a fungus allergen and a food allergen. According to the diagnostic kit, the difference of the sensitivities of different proteins in one diagnostic kit is overcome, and the detection sensitivity of each allergen is enhanced to the maximum extent by repeatedly testing and adjusting the coating amount of the allergens, the concentration of the enzyme-labeled antibody and the formulae and the concentrations of the chemiluminiscence substrate solutions. The chemiluminiscence diagnostic kit is higher in detection sensitivity, safe, reliable, simple and convenient to operate and low in cost in comparison with the ELISA (enzyme linked immunosorbent assay), western-blot and RAST (radioallergo-sorbent test) diagnostic kits for the allergens.

A method of applying a topical preparation of at least one of a mast cell stabilizer, an antihistamine, and a leukotriene inhibitor is disclosed for prevention and / or treatment of oral allergy syndrome of the lips, including lip itchiness and / or swelling. For example, topical application of Cromolyn Sodium to the lips can be used to prevent and / or treat allergic reaction to consumption or other contact with raw fruits and / or raw vegetables. The topical administration can be performed by using applicator devices that apply at least one of a mast cell stabilizer and an antihistamine in the form of a liquid, or a gel, or a butter, or a wax-like solid, or a liposome suspension. Applicator devices can include at least one of: a roller, a brush, a sponge, a swab, a tube, a lipstick. The taste of the at least one of a mast cell stabilizer and an antihistamine can be masked by flavors.

The invention discloses preparation of a bionic molecular imprinting electrochemical sensor and based on click chemistry and a detection method of food allergen. The method includes selecting nitrine alkyl sulfhydryl fixed with the surface of an electrode to prepare a self-assembly single layer film of a nitrine end group, utilizing click chemistry reaction to conduct end group alkenyl functionalization on the self-assembly single layer film on the nitrine end group, selecting a function single body capable of reacting with the allergen to combine molecularly imprinted polymers (MIPs) of an allergen artificial antibody, utilizing the existing method to prepare a graphene material, mixing the graphene material to prepare the artificial antibody, evenly mixing template molecules of the food allergen, the function single body, the graphene, crosslinked agent, pore-foaming agent, initiator and organic solvent according to certain substance amount to prepare the bionic MIPs mingled with the graphene, connecting a prepared MIP modification work electrode to an electrochemical working station and detecting the food allergen in food extraction liquid.

The invention relates to novel compositions and methods for diagnosing or treating food allergies. The invention particularly discloses new approaches for delivering food allergens to allergic patients by oral administration of formulations which dissolve and release proteins in the stomach. The invention allows the treatment of food allergies by delivering food allergens to the gut immune system with controlled exposure of the esophagus or oral cavity. The invention also allows to perform food challenges to assess the threshold of clinical reactivity without exposing the esophagus and oral cavity. The invention may be used in any subject, particularly human subjects, and is applicable to any food allergen.

Assays for detecting antibodies to pharmaceutical preparations, food allergens and environmental allergens are described.

The invention belongs to the technical field of biology and particularly relates to a detecting kit for a specific IgE antibody (quantitative) of a food allergen as well as preparation and detecting methods of the detecting kit. The detecting kit is used for quantitatively detecting the content of the specific IgE antibody (quantitative) of the food allergen in human serum in vitro. The detecting kit for the specific IgE antibody of the food allergen contains the following components: a magnetic separation reagent, an allergen reagent, an enzyme reactant, a calibrated product, a quality control product, a concentrated cleaning solution and a substrate solution, wherein the magnetic separation reagent contains magnetic particles on which human IgE antibodies are coated, and the allergen reagent contains a biotin-labeled specific allergen. The detecting method of the detecting kit for the specific IgE antibody of the food allergen is realized by combining a chemiluminescence immune assay technology and a magnetic particle separation technology, and meanwhile, an enzyme-free capturing method is adopted, so that the detecting method has the advantages of convenience in operation, high sensitivity, good accuracy, high speed and no pollution.

The invention provides a constant temperature amplification detection kit and a method for detecting a food allergen crustacean gene. The kit comprises DNA lysis solution and constant temperature amplification reaction liquid, wherein the constant temperature amplification reaction liquid comprises a forward peripheral primer, a backward peripheral primer, two probes, a cross-species amplification primer, 1*Thermol buffer, MgSO4, dNTPs, Bst DNA polymerase and sterile double-distilled water. The invention also provides the method for detecting the food allergen crustacean gene by using the kit. The method comprises the steps of extracting DNA from a sample to be detected with the DNA lysis solution, performing amplification reaction and detecting. The method for detecting the food allergen crustacean gene has the advantages of accuracy, flexibility and capability of rapidly detecting the food allergen crustacean gene and is suitable for field rapid detection.

The present invention provides a method for detecting food allergens, antibodies and antigens to prepare the antibodies. The antigens of this invention are a mixture comprising multiple native and / or heated food allergens that IgE antibodies of food-allergy patients recognize. The antibodies of this invention are prepared by immunizing animals with the above-mentioned antigens. The food allergen-detecting method of this invention relates to the above-mentioned antibodies. As the method can detect food allergens and food allergy-inducing foods, it can provide safety to food allergy patients.

The invention discloses a real-time fluorescent PCR method for testing the allergen genes of aquatic food, comprising the following steps: total RNA of samples is extracted; the allergen genes Par and TM of aquatic food, and internal standard gene Beta-actin are respectively tested by SYBR Green or TaqMan probe real-time fluorescent PCR technology; and result analysis is carried out to the tested samples according to the amplification kinetic curve and the Ct value. As the real-time fluorescent PCR technology is used for testing the allergen genes Par and TM of aquatic food, compared with other methods, the method has the advantages of high sensitivity, short testing time, available quantitative analysis, and the like, and can be used in the qualitative screening, quantitative analysis and confirmation identification of allergen genes in unknown species or mixed samples of aquatic food.

The invention provides a gene chip and a detection kit for detecting common food allergens, which mainly aim at eight common food allergens of celery, chicken, fish, peanut, sesame, soybean, wheat and almond. The gene chip comprises a solid phase carrier and an oligonucleotide probe which is fixed on the solid carrier and comprises DNA (Deoxyribonucleic Acid) fragments selected from MTD (Maximum Tolerated Dose) gene of celery, mitochondrial DNA of chicken, mitochondrial 16S of fish, Arah1 gene of peanut, Sal gene of sesame, Lectin gene of soybean, GAG56D gene of wheat and Prudul gene of almond. The gene chip and the detection kit can be used for detecting the common food allergens and have the characteristics of being convenience in operation, high flux, high accuracy, high repeatability and the like, and can be used for clinical detection on food anaphylaxis.

The invention discloses a high-sensitivity food allergen detecting method and a detecting kit. The detecting method comprises the following steps: (1) coupling food allergen proteins with magnetic beads, wherein food allergens include but are not limited to food allergen proteins of aquatic products, milk, eggs, meat, cereals, nuts, fruits, vegetables and beans; (2) building a standard curve, namely diluting an anti-food-allergen IgG (Intravenous Gamma Globulin) antibody standard substance in a gradient manner, immunologically reacting the diluted standard substance with the food allergen protein coupled magnetic beads, performing signal amplification by an anti-human IgG secondary antibody, and performing linear regression according to an average absorbance value and corresponding concentration to build the standard curve; and (3) screening food allergens, namely immunologically reacting the food allergen protein coupled magnetic beads with serum of an allergy patient, performing signal amplification by the anti-human IgG secondary antibody, and then determining the types of the food allergens as well as the concentration of the corresponding antibody. The range of sensitivity is not higher than 2pg / mL, and the method and the kit can be applicable to detection on allergens of genetically modified foods.

The invention relates to a food allergen wheat component LAMP (loop-mediated isothermal amplification) field quick detection method. The invention discloses a food allergen wheat component LAMP detection method for the first time. The invention uses wheat GAG56D gene as a target gene for identification, and designs LAMP amplification primers with favorable specificity. The method provided by the invention can be well used for identifying the food allergen wheat component, and has favorable repeatability and sensitivity.

The invention relates to a specific primer and a kit for detecting common food allergens, and particularly relates to a specific PCR (Polymerase Chain Reaction) primer for detecting food allergens and a method and application thereof for rapidly detecting the common food allergens with a multiplex-PCR kit. The kit comprises a 10*PCR reaction solution, MgC12, dNTP (Diethyl-Nitrophenyl Thiophosphate), a primer and DNA (Deoxyribonucleic Acid) polymerase, wherein the primer comprises the following specific nucleotide sequences: (1) MTD (Maximum Tolerated Dose) gene of celery, (2) mitochondrial 16S of fish, (3) Lectin gene of soybean and (4) Prudul gene of almond. The specific nucleotide, the PCR kit with the nucleotide, and the gene chip or a micro-array which are used for detecting the common food allergens are high in practicability; and the PCR kit is simple and convenient to prepare, needs a short detection period, has a high speed and high accuracy, is high in operability, can reduce detection costs and is prone to industrial production.

The invention discloses a tester for testing the allergen in the food, which can process qualitative and quantitative test on the allergen of food. Wherein, using buffer liquid to extract food sample to be marked by fluorescence with FITC; arranging the sample on the sample holes of capillary separate test chip; via the affinity capillary electrophoresis that combined with allergen single anti or multi anti of different foods, separating the allergen that needed to be tested; then using capillary electrophoresis to purify more; the capillary electrophoresis part uses coat hollow tube to reduce the protein adsorption of tube wall; at least, using rotary scan laser to induce the fluorescence to process multi-channel parallel test, and using photoelectric multiplier (PMT) to adsorb the fluorescence signal emitted by the sample; the data of PMT via analogue / digital convert card is stored in the control computer; and via relative software working station, the data of each channel is separated to be processed with peak identification and integral, to compare the peak parameter of sample and attain the qualitative and quantitative information of allergen component.

The invention relates to a primer, a probe and a kit of detecting real-time fluorescence PCR of four allergen gluten components in foods and a detection method. The primer and the probe are respectively a detection primer and a detection probe of barley Hordein genes with sequences of SEQ ID NO.1-3, barley Gliadin genes with sequences of SEQ ID NO.4-6, rye Secl genes with sequences of SEQ ID NO.7-9 and oat Avenin genes with sequences of SEQ ID NO.10-12. Based on the condition, the invention discloses the kit for detecting the real-time fluorescence PCR of the food allergen gluten components and the corresponding detection method. The probe and the primer have strong specificity, and the detection kit and the method have simple and easy use, accurate result and high specificity and flexibility.

The invention discloses a food allergen dynamic digestion model and an in-vitro simulation evaluation method. The method mainly comprises the steps of establishing an allergen dynamic digestion modeland an in-vitro simulation evaluation method, simulating a biochemical reaction condition of the stomach and duodenum of a human body through a digestive system simulation device, providing a method for constructing an allergen in-vitro simulated dynamic digestion experiment in the simulated evaluation method, constructing a Caco-2 (human cloned colonic adenocarcinoma cells) small intestine epithelial cell model to simulate small intestine absorption and transport effects, and constructing a KU812 (human peripheral blood basophilic leukemia cells) cell model to evaluate the ability of mast cell degranulation induced by food allergen digestion and transport products. The method of the invention is suitable for evaluating the digestion and sensitization of a purified food allergen or an allergen extracting solution containing a complex food matrix.

The invention discloses establishment and application of a mastocyte-macrophage-coculture-based microfluidic chip, belonging to the field of food quality analysis and detection. Special flow channel design and tensile valve channels are adopted to implement non-direct contact coculture on the two cells, thereby accurately controlling the coculture process of the two cells and effectively researching the cell paracrine secretion mechanism. The electrochemical technique is combined with the microfluidic chip, and electrochemical resistance signals are adopted on a PDMS (polydimethylsiloxane) substrate surface electrogilding electrode to monitor physiological activities of cells in the coculture in real time. By adopting the design above, the cell coculture microfluidic chip platform for food allergen identification evaluation studies is established. The two-dimensional cell PDMS microfluidic chip platform is established and successfully applied to food allergen identification evaluation studies.

The invention discloses a method for screening detected food allergens rapidly and efficiently. Sixteen common allergen ingredients including eggs, milk, peanuts, buckwheat, cashew nuts, pistachio nuts, walnuts, carrots, filbert, almonds, wheat, soybeans, sesame, shrimps and crabs, fish and celery in life are screened and detected simultaneously according to the method. The method includes the steps of extracting DNA (deoxyribonucleic acid) from a sample, subjecting the DNA of the sample to PCR (polymerase chain reaction) amplification detection, screening and detecting the sixteen allergen ingredients simultaneously by single reaction tube through multiple channels of a PCR instrument at a time, and determining the allergen ingredients after two turns of PCR. The method has the advantages that multiple kinds of allergens are screened and detected rapidly at a time, so that a great amount of time can be saved, reagent consumption can be reduced and cost can be lowered.

The invention discloses a group of proteins with allergen activity from metupcllu ensis, a preparation technology thereof and application thereof in medicine, and belongs to the field of natural bioactive substances and preparation technologies and medical applications thereof. The invention provides a group of proteins with allergen activity in a tissue of the metupcllu ensis, and molecular weights of the proteins are 21.6KDa, 36KDa, 46.2KDa, 60KDa, 64.8KDa, 68KDa, 78KDa and 88.5KDa, respectively. The operation according to the invention can obtain main allergen protein compositions of the metupcllu ensis which have high-purity and maintain good immunobiologic activity. The main allergen protein compositions of the metupcllu ensis produced by the invention can be used to prepare diagnostic reagents and desensitizing preparations for clinical shrimp-induced anaphylaxis and prepare reagents used to detect shrimp food allergen.