Chemiluminescence quantitative detection kit for food allergens, and preparation method and detection method thereof

A food allergy and chemiluminescence technology, applied in the field of immunoassay, can solve the problems of pain, high risk, and poor substitution of the subject, and achieve the effects of simple operation, time saving, wide detection range and high precision

Active Publication Date: 2014-07-02
SUZHOU HAOOUBO BIOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology uses magnetized particles suspended by certain substances called allergen or human serum albumin (HSA) instead of traditional reagents like enzymes for detecting specific proteins found on blood cells. These technologies have several benefits over existing methods such as being more sensitive at low concentrations but also easy to use without any extra steps needed beforehand.

Problems solved by technology

Technical Problem: Current methods used for detecting chemical weapons called histamine play important roles in reducing symptoms associated with allergy conditions like respiratory illnesses caused by certain types of substances found naturally within our environment that cause harmful effects including inflammation and autoimmune responses. Existing tests involve invasively administering these agents into humans which may lead to complications ranging from severe side effectings through systematic adverse events. Therefore, it would benefit us to find ways to make non-invasive diagnosis tools more effective without compromising their reliability over existing ones.

Method used

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  • Chemiluminescence quantitative detection kit for food allergens, and preparation method and detection method thereof
  • Chemiluminescence quantitative detection kit for food allergens, and preparation method and detection method thereof
  • Chemiluminescence quantitative detection kit for food allergens, and preparation method and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of kit

[0034] (1) Preparation of magnetic separation reagents:

[0035] Materials and Instruments:

[0036] 1. Magnetic particle: Contains carboxyl group (COOH) active group, the carboxyl group content per gram (g) magnetic particle (dry weight) is 1 millimole (mmol), has superparamagnetism, and the diameter is 1 μm.

[0037] 2. Food allergens: Dissolve 10 mg of freeze-dried peanut allergens in 2 ml of phosphate buffer (pH7.2, 0.02M) to obtain an allergen solution with a concentration of 5 mg / ml. Put the resulting solution into a dialysis bag and fasten it for dialysis After the bag, dialyze with 500ml of phosphate buffer solution at 4°C for 4-8 hours, change the solution 3-4 times, collect the dialyzed product, and the purity of the allergen protein is 90%.

[0038] 3. 2-Morpholineethanesulfonic acid (MES), carbodiimide (EDC), trishydroxymethylaminomethane (TRIS) and other reagents should be chemically pure.

[0039] Steps:

[0040]...

Embodiment 2

[0055] Embodiment 2: the implementation of detection and the evaluation of detection effect:

[0056] (1) Implementation of testing

[0057] Materials and Instruments:

[0058] 1. Magnetic separation reagent and enzyme-labeled reagent, prepared by Example 1.

[0059] 2. IgE calibrators, quality control products, luminescent substrates, cleaning solutions, and specific IgE assay kits (fluorescence method) are produced by Pharmacia.

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Abstract

The invention relates to a chemiluminescence quantitative detection kit for food allergens, which comprises a magnetic separation reagent (a 0.1-1.0 mg/ml food-allergen-labeled magnetic particle suspension) and an ELIASA reagent (a 0.5-1 mu g/ml anti-human IgE antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the food-allergen-labeled magnetic particle suspension and anti-human IgE antibody solution containing alkaline phosphatase label is up to 0.008 IU/ml, and the cross reaction rates of the kit with other immunoglobulins are respectively less than 0.04%; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

Description

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Claims

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Application Information

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Owner SUZHOU HAOOUBO BIOPHARML
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