Chemiluminescence quantitative detection kit for food allergens, and preparation method and detection method thereof

Abstract

The invention relates to a chemiluminescence quantitative detection kit for food allergens, which comprises a magnetic separation reagent (a 0.1-1.0 mg/ml food-allergen-labeled magnetic particle suspension) and an ELIASA reagent (a 0.5-1 mu g/ml anti-human IgE antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the food-allergen-labeled magnetic particle suspension and anti-human IgE antibody solution containing alkaline phosphatase label is up to 0.008 IU/ml, and the cross reaction rates of the kit with other immunoglobulins are respectively less than 0.04%; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

Description

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AI Technical Summary

Benefits of technology

This new technology uses magnetized particles suspended by certain substances called allergen or human serum albumin (HSA) instead of traditional reagents like enzymes for detecting specific proteins found on blood cells. These technologies have several benefits over existing methods such as being more sensitive at low concentrations but also easy to use without any extra steps needed beforehand.

Problems solved by technology

Technical Problem: Current methods used for detecting chemical weapons called histamine play important roles in reducing symptoms associated with allergy conditions like respiratory illnesses caused by certain types of substances found naturally within our environment that cause harmful effects including inflammation and autoimmune responses. Existing tests involve invasively administering these agents into humans which may lead to complications ranging from severe side effectings through systematic adverse events. Therefore, it would benefit us to find ways to make non-invasive diagnosis tools more effective without compromising their reliability over existing ones.

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