Chemiluminescence quantitative detection kit for inhaled allergens, and preparation method and detection method thereof

A Quantitative Detection, Chemiluminescence Technology

Active Publication Date: 2014-07-02
SUZHOU HAOOUBO BIOPHARML
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the skin test has the fatal disadvantages of pain for the examinee, high risk, failure to check during the attack period, and dependence on the judgment of the supervisor. There have been cases of death of patients due to skin tests in Jiangsu Province and Beijing
Moreover, there is currently no legal skin test product approved by the Food and Drug Administration in the country, so its substitution is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemiluminescence quantitative detection kit for inhaled allergens, and preparation method and detection method thereof
  • Chemiluminescence quantitative detection kit for inhaled allergens, and preparation method and detection method thereof
  • Chemiluminescence quantitative detection kit for inhaled allergens, and preparation method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of the kit

[0034] (1) Preparation of magnetic separation reagent:

[0035] Materials and instruments:

[0036] 1. Magnetic particles: containing carboxyl group (COOH) active group, the content of carboxyl group per gram (g) of magnetic particles (dry weight) is 1 millimoles (mmol), with superparamagnetism, diameter of 1μm.

[0037] 2. Inhalation of allergens: Dissolve 10 mg of the lyophilized dust mite allergen in 2 ml of phosphate buffer (pH 7.2, 0.02M) to obtain an allergen solution with a concentration of 5 mg / ml. Put the obtained solution into a dialysis bag and tighten it. After the dialysis bag, perform dialysis with 500ml of phosphate buffer solution at 4°C for 4-8 hours, change the fluid 3-4 times, and collect the dialysis product. The purity of allergen protein is 90%.

[0038] 3. 2-Morpholine ethanesulfonic acid (MES), carbodiimide (EDC), tris (TRIS) and other reagents should be chemically pure.

[0039] Steps:

[0040] 1. Take 100 mg of magneti...

Embodiment 2

[0055] Example 2: Implementation of testing and evaluation of testing results:

[0056] (1) Implementation of testing

[0057] Materials and instruments:

[0058] 1. Magnetic separation reagent and enzyme-labeled reagent were prepared in Example 1.

[0059] 2. IgE calibrators, quality control products, luminescent substrates, cleaning solutions, and specific IgE determination kits (fluorescence method) are produced by Pharmacia.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a chemiluminescence quantitative detection kit for inhaled allergens, which comprises a magnetic separation reagent (a 0.1-1.0 mg / ml inhaled-allergen-labeled magnetic particle suspension) and an ELIASA reagent (a 0.5-1 mu g / ml anti-human IgE antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the inhaled-allergen-labeled magnetic particle suspension and anti-human IgE antibody solution containing alkaline phosphatase label is up to 0.008 IU / ml, and the cross reaction rates of the kit with other immunoglobulins are respectively less than 0.04%; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.

Description

Technical field [0001] The invention relates to the field of immunoanalysis, in particular to a chemiluminescence quantitative detection kit for inhaled allergens, and a preparation method and detection method thereof. Background technique [0002] Chemiluminescence technology is relatively mature in developed countries in Europe and the United States. It is represented by imported brands such as Roche, Abbott, Beckman, and Siemens. It has reliable quality and stable performance. It is mainly developed in domestic tertiary and some secondary hospitals. However, due to the different product characteristics and domestic registration status of each reagent, generally tertiary hospitals will use different series of products from the above manufacturers at the same time. [0003] Allergic diseases, also known as allergic diseases, refer to the body's inhalation, ingestion, injection, or contact with a substance containing allergenic ingredients (called allergens or allergens) that trigg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531G01N21/76
CPCG01N21/76G01N33/53G01N33/535
Inventor 李庆春孙婵丁俊荣宋孟杰李永红左云国
Owner SUZHOU HAOOUBO BIOPHARML
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap