45 results about "Dust mite allergen" patented technology

The invention provides a humidity control system (HCS) to control indoor humidity within a specified range so as to reduce indoor house-dust mite allergen levels. The invention also provides a hydrogel absorber / desorber for reversibly absorbing water vapor from air releasing water vapor to air. The invention employs a reversible hydrogel desiccant as the active element for absorption and desorption of water, thus rendering the HCS more effective and energy-efficient than traditional humidification / dehumidification systems. The HCS of the invention treats indoor air by drawing fresh air in from outside and adjusting the humidity of the air-stream. In certain embodiments, fresh air is drawn in from the outside and mixed with recirculated air. The HCS uses a controller to introduce fresh air into the interior space and to control air circulation within it. Relative humidity is monitored remotely throughout the interior space using wired or wireless sensors.

Methods for producing nucleic acid fragment libraries that express highly diverse peptide domains, wherein the nucleic acid fragments are derived form microorganisms or eukaryotes with compact genomes. Also peptides derived form the libraries wherein the peptides are selected on the basis of their abilities to bind selected target proteins, including c-jun and antibodies raised against dust mite allergens.

Isolated nucleic acids encoding an allergen of Dermatophagoides pteronyssinus, Der p III, are disclosed. A cDNA encoding a peptide having a Der p III activity and a predicted molecular weight of about 24,985 daltons is also described. The nucleic acids can be used as probes to detect the presence of Der p III nucleic acid in a sample or for the recombinant production of peptides having an activity of Der p III. Peptides having an activity of Der p III can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mites.

The invention discloses a method for developing a novel indoor acaricide. The novel acaricide uses clove and other Chinese medicinal materials as raw materials, the main active component thereof is eugenic acid, and the novel acaricide is a pure natural chemical substance, has no notable harms to human bodies and other organic bodies, has no potential hazards to the environment too, and can be widely applied to killing, preventing and controlling various dust mites of dermatophagoides pteronyssinus, dermatophagoides farinae and the like in various regions and various indoor environments. The novel acaricide can effectively control the number of the dust mites in the living environments of people, greatly reduce the density of dust mite allergens in the indoor environments, and significantly reduce the disease incidence of dust mite anaphylactic diseases. Besides, the novel acaricide has outstanding effects of preventing and performing adjuvant therapy on various anaphylactic diseases taking the dust mites as the allergens, such as allelgic asthma, rhinitis and the like.

The invention provides a dust mite allergen and application thereof. The dust mite allergen provided by the invention belongs to a congener of cathepsin D, can be used for diagnosis, treatment and prevention of allergic diseases and especially dust mite allergic diseases, and is especially used for diagnosis, treatment and prevention of allergic diseases caused by the 34th component of the dust mite allergen. The dust mite allergen recombinant protein is prepared by gene cloning and protein expression, and has the advantages of high protein purity, high specificity and high yield. When being used for preparing reagents for diagnosing, preventing or treating allergic diseases caused by the dust mite allergen, the dust mite allergen provided by the invention has the characteristics of high specificity and low cost. Especially, the dust mite allergen can be efficiently used for diagnosing whether the patient is allergic to the 34th component of the dust mite allergen.

The invention discloses a kit and a method for detecting a dermatophagoides pteronyssinus allergen specificity IgE antibody. The kit comprises a calibration material, a quality control material, a biotin-marked dermatophagoides pteronyssinus allergen solution, an alkaline phosphatase-marked mouse-antihuman IgE second antibody solution and a streptavidin-marked nanometer magnetic particle suspension liquid. The kit is utilized to detect the dermatophagoides pteronyssinus allergen specificity IgE antibody. In such a mode, all reagent components in the kit are excellent in stability; the period of validity can reach over one year; the detecting sensitivity is high, the peculiar performance is excellent and the variation is small; a perfect uniform technique is acquired through technical optimization in a large number of experiments; the production is performed in strict accordance with standard production operation specification and quality control regulation; a user can acquire a reliable result by only performing standard operation according to the operation specification; in the clinical research, the conforming relevance with a foreign imported reagent reaches up to over 90% and the cost of the kit is only 1 / 2 of the cost of the foreign imported reagent.

The invention relates to model systems for allergic conditions, and in particular to in vivo model systems in a large animal. The model systems of the invention are especially useful for providing large numbers of activated or non-activated eosinophils, for the discovery and evaluation of novel anti-inflammatory drug targets and for providing a model for the in vivo study of asthma and the effects of allergy treatments. In a preferred embodiment the animal is a sheep. In one embodiment, repeated infusion of house dust mite allergen (HDM) into the mammary gland is used to induce a specific allergic response, which is characterised by the recruitment of inflammatory cells, particularly eosinophils, into the mammary lumen; these cells can be harvested from peripheral blood and mammary lavage (MAL). In a second embodiment, the mammal is immunised with soluble antigen, for example by repeated subcutaneous immunisation, and then subjected to a single challenge with the same antigen administered directly to the lung.

The invention provides a test strip for screening specific dust mite allergen IgE. The test strip is coated with dust mite allergen labeled by colloidal gold. The colloidal gold labeling technique is utilized, the colloidal gold is used for labeling the specific dust mite allergen for the first time, then the labeled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane in a line manner, and the prepared test strip is used for allergen detection, the operation is simple and convenient, the result is accurate, the cost is low and other free detection can be realized.

A method is provided for deactivating a Der-p and / or Der-f allergen which comprises volatilizing into a space to be treated a deactivating amount of a volatile oil selected from cajeput oil (tea tree oil) or an oil comprising one or more terpene hydrocarbons.

The present invention relates to method of preparing dust mite allergen vaccine with nanometer particle as carrier. Dust mite is one of the main allergen causing allergic diseases. The present invention applies biodegradable nanometer PLGA particle as the allergen carrier to coat recombinant dust mite allergen Der p2 / Der f2 in preparing nanometer level dust mite allergen vaccine. Specifically, by means of gene engineering technology, recombinant Der p2 / Der f2 protein is prepared through cloning and expression in colibacillus and PLGA coated nanometer particle is prepared through re-emulsifying and solvent volatizing process. The form, average size, coating rate, medicine carrying amount and extracorporeal accumulation and release curve of the nanometer particle are obtained, and test shows that Der p2 / Der f2-PLGA nanometer particle has certain curative effect on allergic asthma.

The invention provides a dust mite allergen and an application thereof. The dust mite allergen belongs to the TEF (translation elongation factor) protein family, can be used for diagnosing, treating and preventing allergic diseases, particularly dust mite allergic diseases, and is particularly used for diagnosing, treating and preventing the allergic disease caused by a thirty-fifth component of the dust mite allergen. Recombinant protein of the dust mite allergen is prepared through gene cloning and protein expression, the protein purity is high, the specificity is better, and the yield is high. The dust mite allergen is used for preparing a reagent for diagnosing, preventing or treating the allergic disease caused by the dust mite allergen, has the characteristics of high specificity and low cost, and particularly can be used for effectively diagnosing whether a patient is allergic to the thirty-fifth component of the dust mite allergen.

The invention provides a pharmaceutical composition for treating allergic diseases, which comprises a dust mite allergen, a dust mite allergen and a pharmaceutically acceptable carrier. The invention also relates to a preparation method of the pharmaceutical composition. The composition of the invention can be used for the treatment of allergic asthma, allergic rhinitis, allergic dermatitis, chronic urticaria and the like.

The present invention relates to an allergic rhinitis animal model, wherein a mouse allergic rhinitis (AR) animal model is established by using a dust mite allergen protein extract as an allergen, allergizing through peritoneal injection, and exciting through nose dripping. According to the present invention, the method for inducing mouse allergic rhinitis through dust mite protein is provided, and by optimizing the allergizing number, the allergizing doge, the exciting dose and the exciting mode, the mature and stable mouse AR model for treatment drug screening is prepared; compared to the traditional atomization excitation, the nose dripping excitation of the present invention has the following advantages that the allergen consumption at the excitation stage is avoided, and the waste of the allergen is avoided; and the prepared mouse AR model has advantages of low cost, simple operation and good reproducibility, and is the ideal model for studying allergic rhinitis.

The invention discloses a semi-quantitative detection kit for house dust mite-specific IgE and its application. The test kit includes a detection fluorescent liquid and an immunochromatographic test strip for semi-quantitative detection of house dust mite-specific IgE; wherein, the detection fluorescent liquid is obtained by adding carboxyl-containing quantum dot nanospheres on the surface It is covalently coupled with goat anti-human sIgE and mouse anti-rabbit IgG, and prepared after mixing; the immunochromatography test strip includes sequentially connected sample pads, nitrocellulose membranes, water-absorbing pads, and an assembly platform for providing On the lower plastic backing, the nitrocellulose membrane has a test line coated with dust mite allergen protein and a quality control line coated with rabbit IgG, and the sample pad provides a place for the sample to be tested to be added. The semi-quantitative detection of house dust mite-specific IgE can be realized by using the kit established by the invention, which has a good correlation with clinical symptoms, high detection sensitivity, easy to use, and is suitable for large-scale population allergen screening and family detection Wait for the environment.

The present invention comprises a method, materials and apparatus for investigating asthma in humans using dust mite allergen. The dust mite allergen is prepared to be of a controlled size such that particles are respirable, effectively aerosolized, and deliver a significant amount of allergen. The dust mite allergen is applied in a controlled manner within an environmental exposure chamber to elucidate the etiological links between dust mite allergen concentration and asthma response in humans. The environmental exposure chamber is specially-designed to promote homogeneity of allergen concentration. Preferably, the dust mite allergen preparation comprises particles having an average diameter of less than 25 microns, and more preferably 5-10 microns. Correlation between aerosolized particle count and allergen concentration enables “real-time” allergen concentration estimates without the use of expensive and time-consuming assay techniques.

The invention relates to an optimized gene mderp2 obtained by carrying out codon optimization onto a gene of primary allergen Derp2 of the Dermatophagoides pteronyssinus, a recombinant expression plasmid pN8148-SD constructed based on the gene for secreting and expressing allergen Derp2 and recombinant lactococcus lactis L.lactis SD (preservation number as CGMCC (China General Microbiological Culture Collection Center) No.8223). Western blot results show that the recombinant lactococcus lactis L.lactis SD disclosed by the invention can realize secretion and expression of the allergen Derp2; meanwhile, an animal experiment result shows that orally-taken recombinant lactococcus lactis L.lactis SD can increase level of regulatory T cells in a mouse spleen, and can lower anaphylactic reaction caused by dust mite allergen Derp2 in the mouse body, and has a better preventive effect.

This present invention discloses a monoclonal antibody which specifically recognizes and binds to a epitope of group 2 allergen of Dermatophagoides pteronyssiuns, usually named Der p 2, and a hybridoma cell line producing thereof. Furthermore, this invention also discloses a strip, kit and method utilizing said monoclonal antibody for the detection of the presence of dust mite allergens and the calculation of dust mite number in the environment.

The invention relates to combinations of peptides or variants thereof derived from a portion of an amino sequence of a house dust mite allergen, e.g. the allergens Der p 1, Der f 1, Der p 2 and / or Der f 2. Such peptides comprise at least one T cell epitope and a significant high number of patients in a worldwide population will have HLA alleles with the potential to bind the peptides of the peptide combinations. The invention also relates to the use of such peptide combinations in relieving an immune response caused by a dust mite.

The present invention concerns the use of IL-10 as a biological marker for predicting the responsiveness of a house dust mite allergic patient to house dust mite allergen immunotherapy.

The invention relates to dust mite allergen Derf28 and genes and an application thereof, and belongs to the field of biomedicine. A new natural allergen Derf28 in a manner of separating and purifying from the dust mites is obtained, and encoding genes of the allergen are cloned. The dust mite allergen Derf28 disclosed by the invention comprises 659 amino acids. The first-stage structure of the amino acid sequence of the allergen is shown in SEQ ID No.1. The sequences of the encoding genes of the allergens comprise 1980 nucleotides, wherein the serial number of the gene nucleotide sequence in GenBank is KC305502. The dust mite allergen has the following beneficial effects that the dust mite allergen Derf28 and the genes thereof are provided, and the dust mite allergen Derf28 can be applied to preparation of drugs for treating dust mite anaphylactic diseases.

A method is provided for deactivating a Der-p and / or Der-f allergen which comprises volatilizing into a space to be treated a deactivating amount of a volatile oil selected from cajeput oil (tea tree oil) or an oil comprising one or more terpene hydrocarbons.

Vaccination with the DNA encoding T-cell epitopes to the house dust mite Dermatophagoides pteronyssimus (Der p) and Dermatophagoides farinae (Der f were effective in the inhibition of the allergen induced IgE synthesis. Gene therapy using T-cell epitope encoding DNA is useful in combating allergic disease.

The invention discloses a method for detecting dust mite allergen proteins Der f1 and Der p1 and nitrification products thereof in dust. The invention provides the detection method capable of simultaneously determining two main dust mite allergen proteins Der f1 and Der p1 and the nitrification products Nitrated Der f1 and Nitrated Der p1 thereof in the dust. The dust mite allergen proteind and the nitrification products thereof in a dust sample are digested into small-molecular polypeptide through enzymolysis; one polypeptide with the highest abundance is selected as the characteristic polypeptide of the dust mite allergen proteins and the nitrification products of the dust mite allergen proteins, qualitative and quantitative analysis is conducted on the characteristic polypeptide throughultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), and qualitative and quantitative analysis is conducted on the dust mite allergen proteins and the nitrification products of the dust mite allergen proteins through conversion. The method is good in selectivity and high in accuracy and analysis flux, and overcomes the defects that a traditional ELISA (enzyme-linkedimmunosorbent assay) method is low in detection flux, cross reaction of structural analogues possibly exists, and nitroproteins cannot be accurately quantified.

The present invention provides a process for producing dust mite allergen comprising the steps of: (a) constructing a vector that comprises a DNA sequence encoding the dust mite allergen operably linked to a plant-specific promoter; (b) transforming a plant cell or tissue with the vector of step (a); and (c) obtaining the dust mite allergen from the transgenic plant of step (b). A process for producing an antigenic composition and an antigenic composition are also provided.

The invention discloses an indoor botanical miticide as well as a use method and application thereof. The miticide comprises the following components in parts by weight: 2-4.5 parts of motherwort extract, 1-2 parts of white paeony root extract, and 1-2 parts of paeony root extract, with the balance being deionized water. The miticide belongs to a pure natural plant extract, has no harm to human bodies and other organisms, has no residue after being used, and can be widely used for killing dust mites in different indoor environments. Dust mite allergens are main allergens causing allergic reaction diseases, about 80%-100% of asthma patients are allergic to dust mites, and the use of the miticide can effectively control the density of indoor dust mites, so that the allergens related to indoor dust mites are reduced, the occurrence of dust mite allergic diseases is favorably prevented, and effective help is provided for clinical treatment of dust mite allergic diseases.

The invention discloses a rapid detecting room dust mite allergen second group constituent content florescent real time ration PCR method. The method utilizes Der f 2 reorganized granule as the standard, designing the Der f 2 real time florescent ration PCR primer, and TaqMan probe, adopting the real time florescent TaqMan probe ration PCR method, through the construction of Der f 2 real time florescent ration PCR standard curve, to realize a rapid ration detection of the room dust mite allergen Der f 2 content. The method applying the environmentally friendly and cheap Der f 2 real time florescent ratio PCR method and commercialized expensive ELISA reagent kit respectively conducts detections on the same room dust samples, the correlation coefficient between the two methods is R = 0.81, P< 0.05, implying the linear relationship of the two methods is excellent.

The invention discloses the field of chemical modification of bovine serum albumin, in particular to a method for enhancing immunity of Lp-PLA2, NGAL and Derf24, mainly using 1,5-pentamethylenediamine and 1,10-decane Diamine-modified bovine serum albumin was used as a carrier protein, which was combined with recombinant human lipoprotein-associated phospholipase A2 protein (Lp‑PLA2), recombinant neutrophil gelatinase-associated lipocalin (NGAL) and recombinant dust mite allergen The Derf24 protein antigen was coupled to prepare the immunogen, which was used to immunize mice, and the antibody titer with higher specific titer was obtained, which was used as a diagnostic reagent.

A process for detecting the content of main allergen Der f2 for dust mite features that the capillary electrophoresis and electrochemical detection technique with long fused quartz capillary tube andthree-electrode system in dectecting pool is used. Its advantages are high effect and high speed.

The present invention provides a modified Dermatophagoides pteronyssinus allergen Der p 5 protein which has ability to inhibit IgE binding when exposed against to the antigen. A method for treating allergy comprising administrating a therapeutically effective dose of the modified D. pteronyssinus allergen Der p 5 protein to a subject suffering from allergy Der p 5 is also provided.