45 results about "Dust mite allergen" patented technology

Methods for producing nucleic acid fragment libraries that express highly diverse peptide domains, wherein the nucleic acid fragments are derived form microorganisms or eukaryotes with compact genomes. Also peptides derived form the libraries wherein the peptides are selected on the basis of their abilities to bind selected target proteins, including c-jun and antibodies raised against dust mite allergens.

Isolated nucleic acids encoding an allergen of Dermatophagoides pteronyssinus, Der p III, are disclosed. A cDNA encoding a peptide having a Der p III activity and a predicted molecular weight of about 24,985 daltons is also described. The nucleic acids can be used as probes to detect the presence of Der p III nucleic acid in a sample or for the recombinant production of peptides having an activity of Der p III. Peptides having an activity of Der p III can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mites.

The invention provides a dust mite allergen and application thereof. The dust mite allergen provided by the invention belongs to a congener of cathepsin D, can be used for diagnosis, treatment and prevention of allergic diseases and especially dust mite allergic diseases, and is especially used for diagnosis, treatment and prevention of allergic diseases caused by the 34th component of the dust mite allergen. The dust mite allergen recombinant protein is prepared by gene cloning and protein expression, and has the advantages of high protein purity, high specificity and high yield. When being used for preparing reagents for diagnosing, preventing or treating allergic diseases caused by the dust mite allergen, the dust mite allergen provided by the invention has the characteristics of high specificity and low cost. Especially, the dust mite allergen can be efficiently used for diagnosing whether the patient is allergic to the 34th component of the dust mite allergen.

The invention discloses a kit and a method for detecting a dermatophagoides pteronyssinus allergen specificity IgE antibody. The kit comprises a calibration material, a quality control material, a biotin-marked dermatophagoides pteronyssinus allergen solution, an alkaline phosphatase-marked mouse-antihuman IgE second antibody solution and a streptavidin-marked nanometer magnetic particle suspension liquid. The kit is utilized to detect the dermatophagoides pteronyssinus allergen specificity IgE antibody. In such a mode, all reagent components in the kit are excellent in stability; the period of validity can reach over one year; the detecting sensitivity is high, the peculiar performance is excellent and the variation is small; a perfect uniform technique is acquired through technical optimization in a large number of experiments; the production is performed in strict accordance with standard production operation specification and quality control regulation; a user can acquire a reliable result by only performing standard operation according to the operation specification; in the clinical research, the conforming relevance with a foreign imported reagent reaches up to over 90% and the cost of the kit is only 1/2 of the cost of the foreign imported reagent.

The invention relates to model systems for allergic conditions, and in particular to in vivo model systems in a large animal. The model systems of the invention are especially useful for providing large numbers of activated or non-activated eosinophils, for the discovery and evaluation of novel anti-inflammatory drug targets and for providing a model for the in vivo study of asthma and the effects of allergy treatments. In a preferred embodiment the animal is a sheep. In one embodiment, repeated infusion of house dust mite allergen (HDM) into the mammary gland is used to induce a specific allergic response, which is characterised by the recruitment of inflammatory cells, particularly eosinophils, into the mammary lumen; these cells can be harvested from peripheral blood and mammary lavage (MAL). In a second embodiment, the mammal is immunised with soluble antigen, for example by repeated subcutaneous immunisation, and then subjected to a single challenge with the same antigen administered directly to the lung.

The invention provides a test strip for screening specific dust mite allergen IgE. The test strip is coated with dust mite allergen labeled by colloidal gold. The colloidal gold labeling technique is utilized, the colloidal gold is used for labeling the specific dust mite allergen for the first time, then the labeled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane in a line manner, and the prepared test strip is used for allergen detection, the operation is simple and convenient, the result is accurate, the cost is low and other free detection can be realized.

The present invention relates to method of preparing dust mite allergen vaccine with nanometer particle as carrier. Dust mite is one of the main allergen causing allergic diseases. The present invention applies biodegradable nanometer PLGA particle as the allergen carrier to coat recombinant dust mite allergen Der p2/Der f2 in preparing nanometer level dust mite allergen vaccine. Specifically, by means of gene engineering technology, recombinant Der p2/Der f2 protein is prepared through cloning and expression in colibacillus and PLGA coated nanometer particle is prepared through re-emulsifying and solvent volatizing process. The form, average size, coating rate, medicine carrying amount and extracorporeal accumulation and release curve of the nanometer particle are obtained, and test shows that Der p2/Der f2-PLGA nanometer particle has certain curative effect on allergic asthma.

The invention provides a dust mite allergen and an application thereof. The dust mite allergen belongs to the TEF (translation elongation factor) protein family, can be used for diagnosing, treating and preventing allergic diseases, particularly dust mite allergic diseases, and is particularly used for diagnosing, treating and preventing the allergic disease caused by a thirty-fifth component of the dust mite allergen. Recombinant protein of the dust mite allergen is prepared through gene cloning and protein expression, the protein purity is high, the specificity is better, and the yield is high. The dust mite allergen is used for preparing a reagent for diagnosing, preventing or treating the allergic disease caused by the dust mite allergen, has the characteristics of high specificity and low cost, and particularly can be used for effectively diagnosing whether a patient is allergic to the thirty-fifth component of the dust mite allergen.

The invention discloses a semi-quantitative detection kit for house dust mite-specific IgE and its application. The test kit includes a detection fluorescent liquid and an immunochromatographic test strip for semi-quantitative detection of house dust mite-specific IgE; wherein, the detection fluorescent liquid is obtained by adding carboxyl-containing quantum dot nanospheres on the surface It is covalently coupled with goat anti-human sIgE and mouse anti-rabbit IgG, and prepared after mixing; the immunochromatography test strip includes sequentially connected sample pads, nitrocellulose membranes, water-absorbing pads, and an assembly platform for providing On the lower plastic backing, the nitrocellulose membrane has a test line coated with dust mite allergen protein and a quality control line coated with rabbit IgG, and the sample pad provides a place for the sample to be tested to be added. The semi-quantitative detection of house dust mite-specific IgE can be realized by using the kit established by the invention, which has a good correlation with clinical symptoms, high detection sensitivity, easy to use, and is suitable for large-scale population allergen screening and family detection Wait for the environment.

The present invention comprises a method, materials and apparatus for investigating asthma in humans using dust mite allergen. The dust mite allergen is prepared to be of a controlled size such that particles are respirable, effectively aerosolized, and deliver a significant amount of allergen. The dust mite allergen is applied in a controlled manner within an environmental exposure chamber to elucidate the etiological links between dust mite allergen concentration and asthma response in humans. The environmental exposure chamber is specially-designed to promote homogeneity of allergen concentration. Preferably, the dust mite allergen preparation comprises particles having an average diameter of less than 25 microns, and more preferably 5-10 microns. Correlation between aerosolized particle count and allergen concentration enables “real-time” allergen concentration estimates without the use of expensive and time-consuming assay techniques.

The invention relates to an optimized gene mderp2 obtained by carrying out codon optimization onto a gene of primary allergen Derp2 of the Dermatophagoides pteronyssinus, a recombinant expression plasmid pN8148-SD constructed based on the gene for secreting and expressing allergen Derp2 and recombinant lactococcus lactis L.lactis SD (preservation number as CGMCC (China General Microbiological Culture Collection Center) No.8223). Western blot results show that the recombinant lactococcus lactis L.lactis SD disclosed by the invention can realize secretion and expression of the allergen Derp2; meanwhile, an animal experiment result shows that orally-taken recombinant lactococcus lactis L.lactis SD can increase level of regulatory T cells in a mouse spleen, and can lower anaphylactic reaction caused by dust mite allergen Derp2 in the mouse body, and has a better preventive effect.

This present invention discloses a monoclonal antibody which specifically recognizes and binds to a epitope of group 2 allergen of Dermatophagoides pteronyssiuns, usually named Der p 2, and a hybridoma cell line producing thereof. Furthermore, this invention also discloses a strip, kit and method utilizing said monoclonal antibody for the detection of the presence of dust mite allergens and the calculation of dust mite number in the environment.

The present invention concerns the use of IL-10 as a biological marker for predicting the responsiveness of a house dust mite allergic patient to house dust mite allergen immunotherapy.

The invention relates to dust mite allergen Derf28 and genes and an application thereof, and belongs to the field of biomedicine. A new natural allergen Derf28 in a manner of separating and purifying from the dust mites is obtained, and encoding genes of the allergen are cloned. The dust mite allergen Derf28 disclosed by the invention comprises 659 amino acids. The first-stage structure of the amino acid sequence of the allergen is shown in SEQ ID No.1. The sequences of the encoding genes of the allergens comprise 1980 nucleotides, wherein the serial number of the gene nucleotide sequence in GenBank is KC305502. The dust mite allergen has the following beneficial effects that the dust mite allergen Derf28 and the genes thereof are provided, and the dust mite allergen Derf28 can be applied to preparation of drugs for treating dust mite anaphylactic diseases.

The invention discloses a method for detecting dust mite allergen proteins Der f1 and Der p1 and nitrification products thereof in dust. The invention provides the detection method capable of simultaneously determining two main dust mite allergen proteins Der f1 and Der p1 and the nitrification products Nitrated Der f1 and Nitrated Der p1 thereof in the dust. The dust mite allergen proteind and the nitrification products thereof in a dust sample are digested into small-molecular polypeptide through enzymolysis; one polypeptide with the highest abundance is selected as the characteristic polypeptide of the dust mite allergen proteins and the nitrification products of the dust mite allergen proteins, qualitative and quantitative analysis is conducted on the characteristic polypeptide throughultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and qualitative and quantitative analysis is conducted on the dust mite allergen proteins and the nitrification products of the dust mite allergen proteins through conversion. The method is good in selectivity and high in accuracy and analysis flux, and overcomes the defects that a traditional ELISA (enzyme-linkedimmunosorbent assay) method is low in detection flux, cross reaction of structural analogues possibly exists, and nitroproteins cannot be accurately quantified.

The present invention provides a process for producing dust mite allergen comprising the steps of: (a) constructing a vector that comprises a DNA sequence encoding the dust mite allergen operably linked to a plant-specific promoter; (b) transforming a plant cell or tissue with the vector of step (a); and (c) obtaining the dust mite allergen from the transgenic plant of step (b). A process for producing an antigenic composition and an antigenic composition are also provided.

The invention discloses a rapid detecting room dust mite allergen second group constituent content florescent real time ration PCR method. The method utilizes Der f 2 reorganized granule as the standard, designing the Der f 2 real time florescent ration PCR primer, and TaqMan probe, adopting the real time florescent TaqMan probe ration PCR method, through the construction of Der f 2 real time florescent ration PCR standard curve, to realize a rapid ration detection of the room dust mite allergen Der f 2 content. The method applying the environmentally friendly and cheap Der f 2 real time florescent ratio PCR method and commercialized expensive ELISA reagent kit respectively conducts detections on the same room dust samples, the correlation coefficient between the two methods is R = 0.81, P< 0.05, implying the linear relationship of the two methods is excellent.

A process for detecting the content of main allergen Der f2 for dust mite features that the capillary electrophoresis and electrochemical detection technique with long fused quartz capillary tube andthree-electrode system in dectecting pool is used. Its advantages are high effect and high speed.