7 results about "House dust mite" patented technology

The invention discloses a construction method and application of a machin allergic rhinitis animal model, and belongs to the field of disease animal model construction. According to the method, house dust mites are firstly used for sensitization, then 10% hydrogen peroxide and house dust mites are combined for excitation modeling, the 10% hydrogen peroxide destroys nasal mucosa and accelerates occurrence of allergic rhinitis, and the monkeys with allergic rhinitis are obtained after 13 periods of modeling. According to the method, the animal model which is highly similar to human allergic rhinitis in behavior, inflammatory symptom and phenotype is successfully constructed. The model provides guarantee for observing nasal symptoms and rhinitis behaviors of allergic rhinitis, inducing an organism to generate allergy-related immune response, establishing a sensitization basis, simulating a pathogenesis process, researching pathogenesis of allergic rhinitis, screening allergic rhinitis drugs and the like.

The invention discloses a construction method of an animal model for combined induction and exacerbation of asthma. The method comprises the following steps: injecting ovalbumin or dermatophagoides pteronyssinus extract into an intraperitoneal cavity for sensitization; aerosol inhalation of allergen is carried out for airway re-exposure; in the aggravating stage, pathogenic microorganisms such as pseudomonas aeruginosa, aspergillus fumigatus or respiratory syncytial virus are given and exposed to 500 mu g / m PM2.5 aerosol; lung tissue and bronchoalveolar lavage fluid are then detected. The established model shows that the airway resistance is obviously enhanced and persistent, the mucous thrombus formation rate is greater than 50%, the smooth muscle is obviously thickened, and the model is accompanied by eosinophilic granulocyte and neutrophil infiltration and high expression of various inflammatory factors. The model is good in stability and high in repeatability, can truly simulate clinical asthma acute exacerbation and steroid insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The invention relates to the field of allergen detection, in particular to an anti-Der p 2 antibody and application thereof. The invention provides an anti-Der p 2 antibody. A heavy chain variable region of the anti-Der p 2 antibody comprises an amino acid sequence of a complementarity determining region: CDR1 as shown in SEQ ID No.1, CDR2 as shown in SEQ ID No.2, and CDR3 as shown in SEQ ID No.3; or the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.8. According to the protein, a New Zealand rabbit is immunized by utilizing Der p 2 protein of a main allergen of Dermatophagoides pteronyssinus, and a group of monoclonal antibodies with high affinity and high sensitivity to Der p 2 are obtained through screening. The antibody is further subjected to humanized IgE modification and is applied to a magnetic particle chemiluminescence quantitative detection reagent of Der p 2 specific IgE. According to the reagent, a standard curve for Der p 2 independent calibration can be established, so that accurate and repeatable absolute quantitative analysis is realized, and the detection sensitivity can reach 0.1 IU / mL. The detection method formed on the basis of the anti-Der p 2 antibody provided by the invention has important value for clinical diagnosis of anaphylactic reaction.

The invention relates to a method for obtaining a probiotic product for neutralizing allergens, released by house dust mites, product composition and method of use, which is from the technical field of providing a healthy microclimate based on a probiotic product. The product concerns the maintenance of household stuff in immediate and direct use by humans, such as shoes, blankets, upholstered furniture, clothes, carpets and mats, the air in domestic and office premises, as well as in bathrooms, toilets and vehicles. The product composition consists of a spore concentrate, containing minimum of 1x109 CFU / ml fermentation bacteria class 1 (one), ethanol, hypoallergenic perfume mixture, preservative and water, softened, processed by reverse osmosis and cavitation, ultra-filtered, and microbiologically clean in terms of bacteria and viruses.

The present invention relates to a pharmaceutical composition for preventing, treating or ameliorating respiratory diseases, comprising an Agrimonia pilosa extract as an active ingredient. The pharmaceutical composition can alleviate inflammatory responses occurring in lung tissues and reduce allergic responses, and thus can be effectively used for preventing, ameliorating or treating various respiratory diseases, particularly lung diseases caused by house dust mites.

The application discloses a kind of construction methods of combined induction aggravation asthma animal model.The method includes: abdominal cavity injection egg white protein or house dust mite extract is sensitized;Allergen is inhaled by atomization and is exposed to airway;In aggravation stage, give pseudomonas aeruginosa, lung fumagine or respiratory syncytial virus and expose to 500 μg / m 3 PM2.5 aerosol;Subsequently, lung tissue and bronchoalveolar lavage fluid are detected.The model shows that airway resistance is significantly enhanced and continues, the rate of mucus plug formation is greater than 50%, smooth muscle is significantly thickened, accompanied by eosinophil and neutrophil infiltration and high expression of various inflammatory factors.The model of the application has good stability, strong repeatability, can truly simulate clinical asthma acute exacerbation and steroid-insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The present invention provides a composition and method for treating an allergic disease. The composition comprises a ligand for asialoglycoprotein receptor 1 (Asgr1). The allergic disease may be atopic dermatitis, allergic rhinitis, urticaria, allergic asthma, allergic conjunctivitis, allergic gastrointestinal inflammation, or anaphylactic shock. The allergic disease may be caused by house dust mites. The present invention also provides a method for determining if a test compound activates human Asgr1.