The invention discloses a preparation method of a
hypoallergenic acellular matrix implant, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: swelling animal soft tissues in an alkaline solution for a short time, quickly freezing and crushing; placing the crushed sample in a hypertonic-hypotonic
solution system, and performing efficient and mild
decellularization treatment through circulating oscillation; then, endotoxin and other sensitizers are specifically removed by adopting a
chromatography technology in sequence, denatured
protein is washed by utilizing a
salt solution, and finally, purification is performed through
ultrafiltration or
dialysis. According to the method,
cell debris,
nucleic acid and endotoxin can be deeply removed through multi-step
synergy and combination of hypertonic-hypotonic circulating
decellularization and chromatographic purification, the
immunogenicity of an
implant is remarkably reduced, and the natural three-dimensional structure and
biological activity of an
extracellular matrix are reserved to the maximum extent. The
implant prepared by the method has the characteristics of low
DNA residual quantity, extremely low endotoxin content and excellent
biocompatibility, and is suitable for
soft tissue repair and regeneration.