The invention provides nutrient peanut butter and a preparation method of the nutrient peanut butter. The nutrient peanut butter comprises the following ingredients in weight part: 50-70 parts of peanuts, 10-20 parts of sesames, 10-20 parts of cashew nuts, 8-15 parts of walnuts, 5-10 parts of pine nuts, 20-30 parts of honey, 6-8 parts of apples, 6-8 parts of bananas, 3-5 parts of carrots, 4-9 parts of broccoli, 4-6 parts of soybean oil, 0.5-1.2 parts of salt, 0.3-0.6 part of chicken powder, 0.8-1.1 parts of white vinegar, 0.8-1.3 parts of cooking wine, 4-7 parts of garlic, and 0.2-0.4 part of ginger. The preparation method comprises the steps of washing and cutting the apples, bananas, carrots and broccoli into particles, then putting the other ingredients in a stirrer for stirring, and then blending the fruit and vegetable particles with the mixture. As the fruit and vegetable particles are added in the composite peanut butter, the peanut butter is high in nutritional value, special in flavor, fine and smooth in taste, simple and convenient to manufacture, low in manufacturing cost, and suitable for large-scale production.