230 results about "Birdsrape Mustard" patented technology

The invention relates to a technique of remediation of slightly and medio- metal-contaminated vegetable soil with production. The specific steps are as follows: firstly, Sedum alfredii and Elsholtzia splendens are intercropped in vegetable soil contaminated by heavy metal such as cadmium, copper and zinc in spring with a with inter plant distance of ten to fifteen centimeters, row spacing of thirty centimeters; secondly, the Sedum alfredii is harvested periodically, a growth period of Sedum alfredii is three to four months during a first harvest, from then on, the Sedum alfredii is harvested every three months or when growing to a height of thirty to forty centimeters, stems of a height of two to three centimeters are left above the ground in harvest; the Elsholtzia splendens is harvested in October; thirdly, a cadmium-enriched rape is planted after the harvest of the Elsholtzia splendens with the spacing in the row of fifteen to twenty centimeters and is harvested in April of the next year; fourthly, a cucumber is planted after the harvest of the rape with the spacing in the row of twenty to twenty-five centimeters, a cabbage is planted after the harvest of the cucumber in September, the Elsholtzia splendens is planted after the harvest of the cabbage, then a restoring period is completed. Adopting a super accumulator plant for absorbing a plurality of heavy metal from the soil, the invention achieves reducing the heavy metal content in the vegetables and guaranteeing the safety of the farm produce.

The invention discloses a multi-functional composite conditioner for controlling cadmium-polluted soil and a remediation method for soil cadmium pollution. The multi-functional composite conditioner comprises an inorganic passivation agent for soil heavy metal cadmium, an organic passivation agent and a cadmium antagonist, wherein the inorganic passivation agent is selected from red mud, hydroxyapatite, bentonite, zeolite or dolomite; the organic passivation agent comprises cruciferous plant residues or liliaceous plant residues. The cruciferous plant residues are Chinese cabbage plant residues, rape plant residues, leaf mustard plant residues or cabbage plant residues, and the liliaceous plant residues are garlic plant residues, onion plant residues or Chinese chive plant residues. The cadmium antagonist is a compound containing Zn, Ca or Fe. The multi-functional composite conditioner adopts a composite cadmium remediation mode, can reduce the content of cadmium in plant bodies, and provides an effective, feasible and economic means for the planting of agricultural products with normal content of cadmium.

The invention discloses nitrilase from brassica rapa, encoding genes and an application of the encoding genes to the preparation of pregabalin chiral intermediate (S)-3-cyan-5-methylhexanoic acid through biocatalysis, and an amino acid sequence of the nitrilase is shown as SEQ ID NO:1. The invention provides novel nitrilase for preparing the (S)-3-cyan-5-methylhexanoic acid with high region and high stereoselectivity through hydrolysis, the concentration of hydrolysis substrates of the nitrilase can reach more than 1 M, and an ee (enantiomeric excess) value is kept more than 99 percent. The pregabalin important chiral intermediate (S)-3-cyan-5-methylhexanoic acid can be prepared through the nitrilase; a preparation method of the (S)-3-cyan-5-methylhexanoic acid has the advantages of high atom economy, mild conditions, environmental friendliness and the like and has high industrial application potential.

The invention relates to a usage of Brassica rapa polysaccharide which is extracted from Brassica rapa plants in the preparation of anti-hypoxia drugs, the average molecular weight of the Brassica rapa polysaccharide is 30,000-80,000, and the total polysaccharide content is 60-95 percent by weight. The Brassica rapa polysaccharide can be prepared into pharmaceutical acceptable formulations, such as granules, oral liquid, capsules, tablets, injections or powder. The Brassica rapa polysaccharide obtained by the method has available raw materials, simple method, low production cost, high polysaccharide content in the product, stable quality and easy control. The Brassica rapa polysaccharide has obvious anti-hypoxia activity. The Brassica rapa polysaccharide can be used for preparing the drugs or health care foods for prevention or / and treatment of low oxygen, hypoxia, hemic hypoxia, circulatory hypoxia and toxic hypoxia.

Disclosed is peanut sauce with tuber magnatum and a preparation method thereof. Raw materials of the sauce comprise, by weight, 80-90 parts of peanuts, 3-4 parts of acanthopanax, 2-3 parts of perilla leaves, 3.5-4 parts of camellia sinensis (L.) O.Kuntzethea sinensis L., 4-5 parts of motherwort, 4.5-5.5 parts of elderberry flowers, 1-2 parts of pleurospermum hookeri C.B.Clarde var. thomsonii C.B.Clarke, 2-3 parts of viola formosana hayata, 10-12 parts of cherries, 20-23 parts of Chinese cabbage, 10-11 parts of pistachio nuts, 1-1.2 parts of tuber magnatum, 4-5 parts of beer, 7-8 parts of dried shrimps, 2-3 partos of oyster sauce, 15-17 parts of shiitake mushrooms and 4-5 parts of nutrition additives. The peanut sauce is fine in mouthfeel, simple in preparation technology, and reasonable and scientific in formula. With the added tuber magnatum, the sauce is rich in fragrance and is unique in flavor. With the pistachio nut which is rich in vitamin E and fat, the sauce has effects of antisenescence and moistening the intestines and relaxing bowel. In addition, with addition of various Chinese herbal medicines, the sauce has effects of activating blood and freeing collateral vessels, moving qi and harmonizing the middle, and tonifying kidney and strengthening stomach.

The invention relates to a pesticide, and especially relates to a complex bactericide composed of bacillus subtilis and a chemical bactericide. The bactericide is used for controlling brassica rapa pekinensis downy mildew. The bactericide comprises the chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, and auxiliary materials. The bactericide is characterized in that the chemical bactericide in the active components is any one selected from metalaxyl, propamocarb, and mancozeb. A biocontrol bacterium is bacillus subtilis XM16, with the strain collection number of CGMCC No.0372. A weight ratio of metalaxyl to bacillus subtilis XM16 is 1-8:1-10. A weight ratio of propamocarb to bacillus subtilis XM16 is 1-10:15-140. A weight ratio of mancozeb to bacillus subtilis XM16 is 1-15:1-10. A form of the bactericide is usually hydrophilic powder or aqueous solution. The complex agent of the biocontrol bacterium and the chemical bactericide has a substantial synergetic effect in controlling brassica rapa pekinensis downy mildew. The biocontrol bacterium bacillus subtilis XM16 is advantaged in high bacteriostatic activity, high stress resistance, and wide bactericidal spectrum. The biocontrol bacterium is complexed with the oomycetes chemical bactericide, such that the pesticide dosage is reduced, and controlling effect is improved. The bactericide is an environment-friendly bactericide. The bactericide is advantaged in high biological safety, low cost, and substantial economic benefit.

The invention discloses a feed for raising stone frog, which contains the following materials: (1) feeds for hatched tadpole: selenium-enriched tea, pumpkin powder, sweet potato flour and rice; (2) feeds for transformed froglet: yellow mealworm, barley pest, silkworm chrysalis meal, earthworm, Chinese cabbage, corn flour, bitter chinaberry leave and Aster striatus, wherein yellow mealworm, barley pest and earthworm by weight parts are aired under the blazing sun until being dry; the dried yellow mealworm, barley pest and earthworm are mixed with silkworm chrysalis meal, Chinese cabbage, corn flour, bitter chinaberry leave and Aster striatus by weight parts, 2-3 times of water is added, and the mixture is ground and dried at normal temperature; and the feed formula disclosed by the invention is formed by conventional and economical materials, thereby meeting the requirements that the actual growth of stone frog needs to be scientific and economical, and improving the output and quality of the raised stone frog.

The invention relates to a molecular marker method of a non-heading Chinese cabbage turnip-mosaic-virus-resistance gene, including screening and cultivating of turnip-mosaic-virus-resistance non-heading Chinese cabbage parent materials, parent hybridization and F1 selfing, vaccination and testing of turnip mosaic viruses, DNA extraction of non-heading Chinese cabbage materials and the establishment of a resistance pool, the molecular marker screening of resistance genes, calculation of exchange rate and distance of resistance gene linked markers and establishment of a resistance gene linked marker map and the like; wherein, the molecular marker technology is applied to screen the parents of the turnip-mosaic-virus-resistance non-heading Chinese cabbage and the marking of the linked genes of the resistance genes; and different molecular markers have different reaction systems and amplification procedures. The method constructs the molecular marker procedures and systems of turnip-mosaic-virus-resistance genes with non-heading Chinese cabbage as material and provides bases for positioning the non-heading Chinese cabbage turnip-mosaic-virus-resistance gene on chromosome, resistance gene cloning and assisted breeding research of resistance molecules.

The invention discloses a method for preventing and treating celery cabbage clubroot. The method includes the following steps of 1) species selection and seed pretreatment, 2) sowing, 3) large field soil preparation and fertilization, 4) hole excavation and application of organic bacterial manure and basic converter steelmaking slag mixture, 5) cabbage seedling planting and 6) field management including 6.1) additional fertilizer application and 6.2) pest control. By applying the organic bacterial manure in a large field, pathogenic bacteria can be removed, and beneficial bacteria are multiplied. Micro elements of the basic converter steelmaking slag with M0 larger than 1 are utilized to improve acid soil and prevent and treat the celery cabbage clubroot, application quantity of chemical pesticide can be reduced, and the waste converter steelmaking slag can be turned into wealth. The method is a harmless celery cabbage clubroot preventing and treating technology. By means of the method, celery cabbage yield and quality are improved, and remarkable economical, social and ecological benefits can be obtained.

The invention relates to a health care cigarette. Various vegetables such as spinach, green Chinese onion and Chinese cabbage, wild vegetables such as dandelion, purslane, Athyrium multdentatum (Doell) Ching and vetch (also referred to as Kochia scoparia) as well as tea such as black tea, green tea and flow tea, which are harmless to human body and have health care effect, are taken as main materials. Various seasonings, spices such as dry red pepper, rhizoma zingiberis, fennel, bay leaf, aniseed, pepper, cassia bark and cumin as well as dried fruits such as almond, pistachio nuts and peanuts, which also have health care effect, are taken as auxiliary materials. The auxiliary materials are dried in the sun or dried in the air or dried by fire, and then ground to powder, the powder is mixed with the main materials to be stirred together, and then the mixture can be wrapped to various delicious and refreshing novel health-care cigarettes.

The invention discloses oral liquid which is mainly prepared from Tibetan medicine-food dual-purpose herbal materials, and in particular relates to oral liquid for treating high altitude disease (HAD), which overcomes defects that most of the medicines for treating AHAD need to be taken a few days in advance and have no effect on acute high altitude disease (AHAD) in the prior art. The oral liquid is mainly prepared from brassica rapa linn. juice, hippophae thibetana juice, rheum emodi wall juice, rhodiola crenulata extracting solution and potentilla anserine extracting solution; and the medicine-food dual-purpose herbal materials fully exert efficacy of adjusting physiological functions of a human body by means of reasonable matching of a plurality of components so that the oral liquid has the function of rapidly and efficiently treating HAD. The invention further provides a method for preparing the oral liquid for treating AHAD. The oral liquid can be used for rapidly and effectively relieving adverse symptoms of AHAD and improving sign index without any adverse reaction or toxic or side effect; and the oral liquid is easy to produce and the preparation method is very simple.

The invention relates to pesticide filed, and particularly discloses a new application of a cocklebur fruit water extract in a weeding aspect, namely the cocklebur fruit water extract plays a complete inhibition role for brassica rapa L.Chinensis group under a certain concentration, and can be used as plant biological pesticides and weed killers.

The invention relates to a solid beverage and particularly relates to a brassica rapa L. extract composite solid beverage with a health function. The brassica rapa L. extract composite solid beverage comprises the following components in percentage by mass: 5%-50% of brassica rapa L. extract, 5%-20% of momordica grosvenori powder, 0.5%-3% of tea powder, 20%-85% of fructo-oligosaccharide, 0.1%-0.5% of stevioside and 0.2%-2% of essence. According to the brassica rapa L. extract composite solid beverage, the brassica rapa L. extract is successfully used in the composite solid beverage for the first time; and brassica rapa L. has a health function similar with rhodiola rosea, and can replace rhodiola rosea to a certain extent, so that the strong earthy smell of rhodiola rosea is made up and the development of a food industry is promoted.

The invention provides a pollen-specific promoter and application thereof. The promoter is derived from Brassica rapa and is an upstream sequence of a Delta8 sphingolipid desaturase gene. The promoter provided by the invention is a stable pollen-specific promoter having high specificity. The promoter can be used to promote the expression of various genes in pollen, and can be used for cultivatingmale sterile crop varieties and cultivating landscaping plants and the like with pollen eliminated or greatly reduced.

The invention relates to a seed selection method of a cabbage type brassica juncea cytoplasmic male sterile line. The invention belongs to the technical field of crop seed breeding. The method is characterized in that: a brassica juncea cytoplasmic sterile line WJS1A (AABB) is hybridized with a Chinese cabbage variety of Shanghai cabbage or Beijing cabbage with a genome of AA; in an F2 generation, sterile plants with cabbage type rape characteristics are selected as female parents, and are subject to male parent hybridization with elite cabbage type rape varieties such as Huashuang No.4 (AACC), such that male parent selfed seeds and hybridization seeds are obtained; the seeds are seeded, and F1 plant fertility is assessed during a flowering phase; sterile plants shown infertility combinations are backcrossed for 3-4 generations by using the original male parent, until a cabbage type brassica juncea cytoplasmic male sterile line WNJ01A with stable male sterile characteristics is obtained. The recurrent parent Huashuang No.4 is a maintainance line of the sterile line. As a result of the restoring and maintaining of the relationship, the WNJ01A sterile line is a novel sterile cytoplasm type different from polima cytoplasmic male sterility (Pol CMS), radish cytoplasmic male sterility (Ogu CMS), and Kos cytoplasmic male sterility (Kos CMS). With the method provided by the invention, the obtained WNJ01A sterile line is a novel cytoplasmic male sterile type of rapes and cruciferae crops, and assists in establishing a firm germplasm basis for heterosis utilization of the crops.

A production method of a germplasm resource of ornamental flowering Chinese cabbages is characterized by comprising steps as follows: a conventional variety of common cabbages, that is, Suzhongqing Q8, used as a female parent and Guangdong cabbage selfing line G1 used as a male parent are hybridized to form first hybrid generation seeds F1; first hybrid generation seeds F1 have selfing to form F2; single plants with shortest petioles are selected from F2 to have selfing to form F3; single plants with compact plant types, short petioles and small leaves are selected from F3 to have selfing to form F4; single plants with compact plant types, short petioles, small leaves and glossy dark green cabbage color are selected to have selfing to form F5; single plants with compact plant types, short petioles, small leaves, glossy dark green cabbage color and short joints are selected to have selfing to form F6; single plants with compact plant types, short petioles, small leaves, glossy dark green cabbage color, short joints and upright leaves are selected to have selfing to form F7; single plants with lotus-throne-shaped leaf clusters, unobvious base jointing during cabbage drawing, short stems, long flowering period and large flower amount are selected to has selfing to form F8. The ornamental period of the germplasm leaves and flowers of the ornamental flowering Chinese cabbages is 40-60 days, leaves and flowers can be enjoyed during the New Year's Day and the Spring Festival, the flower amount is large, and the ornamental effect is good.

The present invention provides a composition comprising a green-yellow vegetable (e.g., broccoli, spinach, parsley, komatsuna (Brassica rapa L.), Japanese radish leaves) and a light-colored vegetable (e.g., lettuce, cabbage, celery), the composition having the following effects: (1) inhibiting the generation of blood lipid peroxide; (2) lowering blood TBARS levels or suppressing the elevation of blood TBARS levels; (3) increasing blood vitamin E levels; (4) enhancing blood antioxidant activity; (5) enhancing blood TRAP levels; (6) lowering blood active oxygen levels or suppressing the elevation of blood active oxygen levels; and (7) preventing or treating diabetic complications.

The invention discloses a method for cultivating pakchoi which is highly rich in vitamin C. The method includes the steps of accelerating germination, growing seedlings, transplanting the pakchoi into the land for growing field crops to be harvested, and spraying an ascorbic acid solution onto the pakchoi transplanted into the land for growing field crops to be strengthened before harvesting. According to the method, the Wufengkangreqing variety is preferably selected, the pakchoi with the content of the vitamin C being high can be produced in the short term through the method, the problems happening when artificially synthesized vitamin C preparations are conventionally taken to strengthen vitamin C nutrient of a human body are solved, the content of the vitamin C in the pakchoi is improved through the agronomic measure approach, the dietary structure of the human body is not changed, and the pakchoi can be received by people easily.

The invention discloses a chicken feed for improving meat quality of chicken and a preparation method thereof. The chicken feed is prepared from the following raw materials in parts by weight: 200-250 parts of corn flour, 180-220 parts of soybean flour, 30-40 parts of chicken livers, 20-30 parts of duck blood, 10-20 parts of fish intestines, 1-2 parts of table salt, 80-90 parts of pine leaves, 50-70 parts of pineapple peel, 40-50 parts of Chinese chestnut leaves, 20-30 parts of vanilla juice, 8-9 parts of pear flowers, 5-7 parts of yoghourt, 2-3 parts of glucose, 30-40 parts of bamboo shoots, 40-50 parts of lettuces, 20-30 parts of onions, 3-5 parts of fresh gingers, 7-8 parts of cashew nuts, 10-13 parts of almonds, 20-30 parts of apple seeds, 2-3 parts of schizonepeta, 1-2 parts of divaricate saposhnikovia roots, 2-3 parts of purple bergenia herb, 2-3 parts of gastrodia elata, 10-15 parts of phagostimulant and a proper amount of water. The feed disclosed by the invention is nutritional and safe and green and healthy, the nutrition of chicken is enhanced and the meat quality of chicken is improved, and the chicken meat is fresh and tender, small in muscle fibers, uniform in distribution of chicken fat, rich in flavor and unique in flavor.

The invention belongs to the field of molecular markers and detection thereof and in particular relates to a method for identifying authenticity of a brassica rapa variety and a special SSR (simple sequence repeat) primer combination of the method. An SSR locus for identifying authenticity of a brassica rapa variety is obtained through data digging according to a reference genome Brassica_rapa V1.5 of brassica rapa and a reference genome Brassica olerecea V2.1 of brassica oleracea together with whole genome resequencing data of 40 genetic resources; the SSR primer combination is selected froma first SSR primer pair to a twenty-third SSR primer pair which are respectively applied to PCR (polymerase chain reaction) amplification on a first SSR locus to a twenty-third SSR locus, and are respectively optimally selected as nucleotide sequences which have 85-100% of homology with nucleotide sequences of SEQ ID NO:1-46 in a sequence table. The method can be applied to authenticity identification on the brassica rapa variety for a whole life cycle from seeds, and technical support is provided for protection on genetic resources and new varieties of brassica rapa.

The invention discloses a Lactobacillus plantarum and application thereof in the fermenting cabbage wrapper leaf. The invention provides a Lactobacillus plantarum Z5-5. The Lactobacillus plantarum Z5-5 provided by the invention has the preservation number of CGMCC No.5028 (China General Microbiological Culture Collection Center). The Lactobacillus plantarum Z5-5CGMCC No.5028 can quickly ferment the cabbage wrapper leaf and can effectively inhibit escherichia coli. The Z5-5 is inoculated by 5% to ferment the cabbage wrapper leaf; the cabbage wrapper leaf is fermented for 24h at 30DEG C; the pH of fermented vegetable juice is 2.07; and no escherichia coli can be detected. Compared with natural fermentation, Z5-5CGMCC No.5028 is inoculated to ferment the cabbage wrapper leaf; the content of various types of amino acids is increased to different degrees; the total amount of amino acid is increased by 16.03%. Thus, nutritional value is obviously improved.

The invention discloses a making method of spicy cabbages preserved with microorganism bacteria. The making method comprises the following specific steps of (1) thoroughly cleaning Chinese cabbages, removing bad leaves from external surfaces, and performing draining to remove water; (2) cutting off roots of the drained Chinese cabbages, and dividing the treated Chinese cabbages into 4 parts; (3) wrapping the Chinese cabbages with gauze, spraying the wrapped Chinese cabbages with spraying liquid, and in the spraying course, performing intermittent infrared radiation; (4) airing the Chinese cabbages after the intermittent infrared radiation, uniformly preserving the aired Chinese cabbages, then putting the preserved Chinese cabbages in jars, adding apple granules, pear granules, the microorganism bacteria, wheat germs, spirulina and a trace element solution, and performing sealed fermentation; and (5) after fermentation is completed, draining the fermented Chinese cabbages, putting the drained Chinese cabbages in a preserving container, then adding a chili sauce, table salt, a fish sauce, minced garlic, ginger granules and chopped scallions, performing sealing, and then performing preservation for 7-13d, so that the spicy cabbages are made. The spicy cabbages made by the making method not only are rich in nutrients and low in nitrite content, but also are complete in five flavorsof acid, sweet, pungent, hot and crisp in mouth feel, people eating the spicy cabbages do not suffer from excessive internal heat easily, and the spicy cabbages are a healthy food being appropriate in mouth feel.

Provided is a cultivation method capable of preventing celery cabbage clubroot diseases from occurring. The accumulated number of soil pathogenic bacteria is increased generally due to the succession cropping cultivation of celery cabbages, and thus the clubroot diseases in soil infection occur seriously. The cultivation method capable of preventing the celery cabbage clubroot diseases from occurring comprises the steps that tiller onions and garlic which are planted in an earlier stage are planted in a every other year rotation mode, and the celery cabbages are planted at the identical positions in an automatic crop stage; plant ash applied before the celery cabbages are planted is required to be freshly made when used, and the plant ash is prevented from being wetted by rainwater before being applied; routine management is carried out until the celery cabbages are harvested. The cultivation method capable of preventing the celery cabbage clubroot diseases from occurring is applied to open field celery cabbage production in the autumn crop stage.

The invention relates to a zinc-rich Chinese cabbage cultivating method. The zinc-rich Chinese cabbage cultivating method includes soaking seeds before sowing by seed soaking fluid made by aloe juice, distilled vinegar and water. The seed soaking fluid can treat and kill downy mildew and black rot, germination rate of seeds is increased, morbidity of downy mildew and black spot is decreased during cultivation, shell powder, compost peanut shells and boggy soil are used as cultivating soil, nutrient is adequate, and leaf rolls and wrappage due to insufficient nutrient are avoided during cultivation. Without additional soil fertilizer during cultivation, cultivating cost is saved, specially made leaf fertilizer sprayed during the seedling stage, the pod stage and the heading stage can promote growth of Chinese cabbage, the cabbage has compact inside, cabbage leaves have high water content, natural withering of the leaves is prevented, soft rot caused by natural breaches is reduced, disease resistance of the cabbage is improved, yield of cabbage is increased by more than 30% as compared with that of a common cultivation technology, soft rot and tip burn of the cabbage are avoided, incidence of the mature cabbage is reduced to 2% during the cultivation stage, and zinc content of the ripen cabbage in each 100 g is up to 8.32 to 9.01 mg.

The invention discloses a medicine for treating parasitic diseases of pigs and a preparation method thereof. The medicine comprises the following raw medicinal materials: Mexican tea herb, cyrtomium rhizome, dark plum fruit, pomegranate root, bigfruit Elm product, bark of David christmashush, areca seed, pumpkin seed, herb of Wilson passionflower, amur corktree bark, stem and leaf of bird rape, seedling in cushaw, pepperweed seed, leaf of betel pepper, honeysuckle stem, heterophylly falsestarwort root, rangooncreeper fruit, radix astragali, coptis root, malt sugar, honey, bistort rhizome, common burreed rhizome, zedoray rhizome, aloe, dried tangerine peel, parslane herb and rhizoma dioscoreae. The medicine has the beneficial effects that the medicine has the functions of killing and expelling parasites, has good curative effects mainly on parasitic diseases of pigs, has strong pertinence and direct expelling property, is cheap, is nontoxic and harmless, does not have residues, is safe and convenient, has comprehensive functions and can achieve the effects of improving the physiques of pigs, boosting the immunities of pigs, increasing the survival rate and increasing the market benefits.

The invention discloses an overexpressing brassica napus and application of MYB43 of parental species of the overexpressing brassica napus in improving plant type and increasing yield. The gene familyof the MYB43 of brassica napus comprises BnMYB43-1, BnMYB43-2, BnMYB43-3 and BnMYB43-4 member genes; the parental specie brassica rapa has BrMYB43-1 and BrMYB43-2 member genes; the parental specie brassica oleracea has BoMYB43-1 and BoMYB43-2 member genes. After overexpression in plants, the roots of the plants become more developed and longer, the number of branch roots is more, the ratio of root to crown is increased, the plants are slightly higher, the stems become thicker, the number of effective branches per plant is increased, the number of effective pods per plant is increased, the yield of seeds per plant is increased, and the folding resistance and lodging resistance are also improved significantly. According to the overexpressing brassica napus and the application of the MYB43 of the parental species of the overexpressing brassica napus in improving the plant type and increasing the yield, a new method is provided for application of the MYB43 in improving the plant type, lodging resistance and seed yield of brassica napus, brassica rapa, brassica oleracea and other plants.

The invention discloses SSR molecular marker primers interlinked with a cabbage yellow testa gene Brsc-ye and an application of the primers. The marker primer is prepared by the following steps: with genome DNA of yellow-seed cabbages and ordinary brown-seed cabbage as well as a Fe segregation group thereof as a template, carrying out polymorphic primer screening on a yellow-seed cabbage parent and a brown-seed cabbage parent by using 520 SSR primer pairs, subsequently analyzing single plant of the F2 group, screening molecular markers interlinked with the cabbage yellow testa gene Brsc-ye so as to successfully obtain 7 pairs of SSR marker primers interlinked with Brsc-ye gene, and building a molecular genetic map of the cabbage yellow testa gene Brsc-ye. The interlink analysis discovers that the genetic distances of two SSR markers which are in close linkage with two sides of the Brsc-ye gene are 0.17cM and 0.29cM respectively; the molecular markers have the advantages of convenience in detection, stability in amplification and high in repeatability and accuracy; the reference is provided for molecular-assisted selective breeding of the yellow testa characteristics of cabbage and rape; the seed breeding process is accelerated.

The invention belongs to the technical field of agricultural production, and particularly relates to a cold-resistant agent for brassica rapa L. The cold-resistant agent for the brassica rapa L. seedlings is mainly characterized by comprising raw materials as follows: 15-25 mg / L of ABA (abscisic acid) and the balance of water, wherein the purity of the ABA (abscisic acid) is higher than or equal to 99.9%. The cold-resistant agent aims to solve the problem that the brassica rapa L. in the northern cold and arid regions is vulnerable to low-temperature freezing damage, growth of the overground part and the root system of the brassica rapa L. is promoted with chemical control means, especially the thickening growth of roots, the outstanding performance refers to increase of dry matter accumulation, and safe wintering of the brassica rapa L. is guaranteed; meanwhile, permeability (relative electrical conductivity) of leaf cell membranes as well as accumulation of membrane lipid peroxidation malonaldehyde can be reduced, so that the low-temperature freezing degree of leaves of the brassica rapa L. is reduced, the cold resistance of the brassica rapa L. is remarkably improved, and the cold-resistant agent has great practical significance on brassica rapa L. production.

The invention discloses an InDelmolecular marker KB1 co-separated with a brassica rapa pekinensis clubroot resistance gene Crr1a, a primer and application of the molecular marker. The invention provides a method for identifying or auxiliary identifying whether brassica rapa pekinensis contains the clubroot resistance gene Crr1a or not. The method comprises the steps of adopting a brassica rapa pekinensis genome to be detected as a template; carrying out PCR (Polymerase Chain Reaction) amplification by the primer provided by the invention; according to the size of an amplified product, judgingwhether the brassica rapa pekinensis to be detected contains the clubroot resistance gene Crr1a or not and is the brassica rapa pekinensis with clubroot resistance or not. The molecular marker provided by the invention is utilized for auxiliary selecting the brassica rapa pekinensis with clubroot resistance, is high in accuracy, low in cost, and time and labor saving.