444results about How to "Growth impact" patented technology

The invention discloses a concentric-ring sprayer structure for material vapor phase epitaxy, which solves the problem that the large-area deposition region provides a uniform flow field of a precursor gas mixture in a large-substrate or multi-substrate crystal growth process. The sprayer structure comprises more than one independent air inlet pipeline, wherein each air inlet pipeline is provided with a controller for monitoring and regulating inlet gas flow speed and flow rate; the bottom of the sprayer is provided with an air outlet baffle; more than one concentric ring is arranged in the sprayer; independent cavities are formed among the concentric rings and are mutually separated; the top end of each concentric ring is connected with one independent air inlet pipeline; and the air outlet baffle at the bottom end of each concentric ring is provided with one or more air outlets. The air sources are mutually separated and independently controlled; and the multi-sprayer integrated use mode obviously improves the quality of the large-area deposited grown crystal, and greatly enhances the production efficiency.

The invention belongs to the fields of fruit tree cultivation and fruit tree ecology, and discloses a shaping method for a downward-arrowhead-shaped tree shape of a pear tree. The tree shape has a tree height of 2.4-2.5 m, and a trunk height of 0.6-0.7 m, and only two main branches and one centre trunk of a first layer are reserved, therefore, the tree shape is called as the downward-arrowhead-shaped tree shape. The downward-arrowhead-shaped tree shape is small in top and large in bottom, the influence of upper branches on lower branches is less, and ventilation and light transmission conditions are good. The downward-arrowhead-shaped tree shape of a pear tree is an innovation based on the '3-plus-1' tree shape disclosed previously, which is simple in structure, convenient in culture, easy to grasp by fruit growers, convenient in field management, and easy to spread and popularize; and the tree shape is a novel high-quality and labour-saving cultivation tree shape of a pear tree.

The present invention discloses method of inoculating Guanhua desert cistanche to tamarisk stem. The method includes mainly inducing tamarisk stem to root, inoculating Guanhua desert cistanche to tamarisk root, and maintaining the dark condition after inoculating to form the parasitism between the seed of Guanhua desert cistanche and the root of tamarisk. The present invention has can reach the aim of fast and effectively inoculation of Guanhua desert cistanche, and makes it possible to cultivate desert cistanche in saline alkali soil and tidal land.

The invention relates to the technical field of breeding house air purification, and provides an air purifying system for a breeding house. The system comprises a breeding house body and further comprises an air exchanger and a heating module, wherein a breeding bed is arranged on the inner part of the breeding house body; the air exchanger is arranged on the top of the breeding house body and is used for introducing fresh air; the heating module is used for heating the fresh air which is introduced by the air exchanger; an air outlet is formed in the bottom of the breeding house body. The air exchanger is used for introducing the fresh air outside the breeding house body; moreover, the heating module is used for heating the introduced fresh air; the heated fresh air is positioned in the upper layer of the air in the house, so that the fresh air is supplemented from top to bottom; the original air in the breeding house body is exhausted through the air outlet in the bottom, so that the replacement of air in the breeding house is realized; moreover, the introduced fresh air has been heated, the temperature in the house cannot be reduced during the replacement of the air in the breeding house body, and influences on growth of livestock cannot be caused.

The invention relates to a heavy metal trapping agent in the field of environment protection. The heavy metal trapping agent is prepared from polymer containing a polyethylene diamine tetra-acetic acid unit, wherein by weight percentage, the content of the polyethylene diamine tetra-acetic acid unit is 45-100% of polymer; the polymer is homopolymer or copolymer; the polyethylene diamine tetra-acetic acid unit is a form of sodium salt, potassium salt or ammonium salt. The defect that a chelate compound of existing ethylenediamine tetra-acetic acid and the heavy metal is generally soluble is overcome, and the provided heavy metal trapping agent has strong heavy metal chelating power, and the solubility can be controlled by pH of the solution; moreover, the trapping agent is free of toxicity.

The invention discloses dendrobium nobile accompanying tea. An operation method comprises the first step of seedling hardening, the second step of preparation of matrixes, the third step of laying of the matrixes, the fourth step of transplanting and planting of hardened seedlings, and the fifth step of planting management of the hardened seedlings. The tea obtained through the method equivalently grows in a large environment of dendrobium nobile, flowers are large in number and concentrated and scent the air when dendrobium nobile blooms, and at the same time, the flowers attract many bees and birds to come to gather honey, eat insects and inhabit. As the tea continuously absorbs the fragrance of the flowers of dendrobium nobile in the growing process, some microelements in dendrobium nobile are absorbed into the tea, the natural quality of the tea is improved, and the tea has the effect of strengthening the body.

The invention relates to a soil conditioner for reducing the cadmium and lead pollution to crops, comprising inorganic components and organic components, wherein the effective components of the inorganic components are Ca(OH)2 and Na2SiO7 or mixtures of the Ca(OH)2 and the Na2SiO7; and the organic components include rice straws and tephrosia caudida casts or mixtures of the rice straws and the tephrosia caudida casts. The soil conditioner is used before the crops are cultivated; the inorganic components are prepared into agricultural-grade powder and 0.5-4 g of the inorganic components is used for one kilogram of wind drying soil; and the grain diameter of the crushed organic components is less than 1 cm and 5-40 g of the organic components is used for one kilogram of wind drying soil. The soil conditioner effectively reduces the biological effectiveness of heavy metal, the absorption of cadmium and lead by the crops and the heavy metal pollution to the crops and especially leafy vegetables without reducing the production property of the corps.

The invention relates to a method of interplanning a variety of crops in wheat land, belonging to the method of cultivating crops. The method of interplanning a variety of crops in wheat land comprises the following steps: A, sowing wheat seeds in the left part of the vacant land which is 0.5-1 meter wide, includes a dead furrow of 0.2-0.4 meter wide and is one part of the furrowed land of 2.0-2.5meter wide; B, sowing winter spinach seeds in the edges of the dead furrows; C, harvesting the winter spinach 170-180 days after the sowing of the winter spinach seeds; D, sowing one line of spring corns seeds in the winter spinach stubble land 185-200 days after the sowing of the wheat seeds; E, harvesting the wheat 50-60 days after the sowing of the spring corn seeds; F, sowing two rows of summer corn seeds between the lines of the spring corn 20-30 days after the harvesting of the wheat; G, harvesting the corn 38 days after the sowing of the spring corn; and H, harvesting the summer corn seeds 120-135 days after the harvesting of the spring corn. The invention takes advantage of the potential of the land to additionally harvest the crop which can be harvested after one season growth inthe land of one mu every year, thereby increasing the yield of the crop yield.

The invention relates to an arrangement method of an ecological gabion chamber revetment type, and belongs to a revetment structure type in river training works. A stepped sandstone cushion layer is laid on the revetment of a river; a sinuous ecological gabion chamber is arranged on the stepped sandstone cushion layer; and plants are planted in the sinuous ecological gabion chamber. The arrangement method has the advantages of novel structure, reliable operation, wide application range, capabilities of improving the engineering safety and favorably improving water eco-environments of rivers and lakes and great application prospect.

The invention relates to a special rhodococcus ruber (CGMCC NO. 17012) fermentation method, a preparation process of adjuvants with different dosage forms and an application as an adjuvant in animalvaccines. Rhodococcus ruber strains are separated from a farm and obtained through single colony cloning purification and identification, the invention provides a special fermentation process where the strain inactivation product is used as an animal vaccine adjuvant, and provides a preparation process of different dosage forms of adjuvants containing the strain and the product. Animal experimentsprove that when the adjuvant product is used for univalent or multivalent animal vaccines, particularly Newcastle disease inactivated vaccine, avian influenza inactivated vaccine and swine fever livevaccine, definite non-specific immune enhancement effect can be provided, specifically, the antibody peak value level induced by the animal vaccine is obviously improved, the time for producing the protective antibody level is advanced, the antibody maintenance period is prolonged, and the immune effect of the animal vaccines is enhanced.

The invention discloses a nonferrous mine waste rock storage yard ecological environmental management method. The method comprises the following steps that 1, by means of a slurry spraying machine, apasty neutralization and improvement material is sprayed to the outer surface of a waste rock storage yard, and a neutralization and improvement layer is formed; 2, by means of the slurry spraying machine, a pasty buffer isolation material is sprayed to the outer surface of the neutralization and improvement layer, and a buffer isolation layer is formed; 3, a vegetation recovery layer is formed onthe outer surface of the buffer isolation layer. Accordingly, wet spraying is adopted, a high-concentration mixed material is sprayed by adopting the slurry spaying machine to the waste rock storageyard, and specially for the neutralization and improvement layer, the pasty neutralization and improvement material is better embedded into a gap of an outer surface layer of the waste rock storage yard; compared with existing dry spraying machine spraying, the neutralization and improvement material can be sufficiently embedded, the problem that a large number of gaps are formed does not exist, and therefore due to sufficient embedding, even though the waste rock storage yard deforms slightly, a packing layer is not likely to fail.

The invention provides a split type crucible for crystal growth of silicon carbide. The crucible comprises the following components: a raw material chamber for containing raw materials for crystal growth of SiC; a growth chamber which is nested on the upper part of the raw material chamber in relative movement mode in order to form a crystallization area of crystal, wherein the growth chamber comprises a growth chamber, and a seed crystal holder which is arranged on a upper wall of the growth chamber; and a double layer structure which is formed by sidewalls of the growth chamber and comprises an inner cylinder and an outer cylinder. The crucible can be used for adjusting distance between the crystal surface and the raw material surface in a process of growth, and the stability of a temperature field is kept.

The invention discloses an aluminium nitride single crystal growth device dispensing with a seed crystal bonding technology and a process and relates to the semiconductor manufacture technology. The aluminium nitride single crystal growth device comprises a heating system, an infrared temperature measurement system, seed crystals, a growth crucible, a crucible partition and a double-layered nestedcrucible, wherein the heating system is arranged on the outermost side; a temperature difference exists between the bottom and the top of the crucible; the crucible is arranged in a heat insulation material; the seed crystals are placed at the bottom of the crucible; the double-layered nested crucible is vertically placed on the upper side of the crucible partition and comprises an inner-layer crucible and an outer-layer crucible; the heights of the walls of the inner-layer crucible and the outer-layer crucible are kept the same; and a gap between the side walls of the inner-layer crucible and the outer-layer crucible is filled with high-purity aluminium nitride powder. Doping of aluminium nitride single-crystal impurity elements can be reduced, the crystal quality is improved, the single-crystal usable area is increased, the aluminium nitride single crystal growth device is simple and easy to use, facilitates low-cost preparation of aluminium nitride single crystals, and can preventgrowth of the aluminium nitride single crystals from being influenced by the seed crystal bonding technology.

The invention belongs to the technical field of plant disease and insect pest control, and particularly discloses a method for preventing the citrus tristeza virus. The method is especially suitable for preventing the citrus tristeza virus of fruit bearing production type citrus trees. According to the method, through special management of spring shoots, summer shoots, early and late autumn shoots and winter shoots of the citrus trees every year, the food sources and breeding sites of citrus psylla spreading the citrus tristeza virus can be effectively eliminated so that eggs and nymphs can not grow and develop normally, the number of subcultured pest sources is reduced greatly, and then an ideal preventing effect is realized; meanwhile, the usage amount of insecticides can be reduced, natural enemy protection is achieved, and prevention cost is reduced; furthermore, compared with the shoot control scheme, the method has the advantages that the growth of the citrus trees is not affected, and the effect of eliminating the food sources and breeding sites of citrus psylla spreading the citrus tristeza virus is remarkable.

The invention belongs to the field of pig feeds, and concretely relates to a drug-free growing-fattening pig feed. Every 1000kg of the feed comprises 997.7-999.4kg of low protein ration, 0.1-0.8kg of an enzyme preparation and 0.5-1.5kg of a probiotic-functional oligosaccharide composition, wherein the low protein ration meets a condition that the content of proteins in the feed is not greater than 15.0%, the enzyme preparation comprises xylanase, mannanase and cellulase, and the probiotic-functional oligosaccharide composition comprises Bacillus subtilis, Clostridium butyricum, Bacillus licheniformis and Chitosan oligosaccharide. The feed disclosed in the invention solves a problem of use of antibiotic drugs in feeds, starts with raw material utilization and intestinal tract flora health, has a good growth performance effect, and brings good benefits to raising households.

The invention relates to a pesticide, and especially relates to a complex bactericide composed of bacillus subtilis and a chemical bactericide. The bactericide is used for controlling brassica rapa pekinensis downy mildew. The bactericide comprises the chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, and auxiliary materials. The bactericide is characterized in that the chemical bactericide in the active components is any one selected from metalaxyl, propamocarb, and mancozeb. A biocontrol bacterium is bacillus subtilis XM16, with the strain collection number of CGMCC No.0372. A weight ratio of metalaxyl to bacillus subtilis XM16 is 1-8:1-10. A weight ratio of propamocarb to bacillus subtilis XM16 is 1-10:15-140. A weight ratio of mancozeb to bacillus subtilis XM16 is 1-15:1-10. A form of the bactericide is usually hydrophilic powder or aqueous solution. The complex agent of the biocontrol bacterium and the chemical bactericide has a substantial synergetic effect in controlling brassica rapa pekinensis downy mildew. The biocontrol bacterium bacillus subtilis XM16 is advantaged in high bacteriostatic activity, high stress resistance, and wide bactericidal spectrum. The biocontrol bacterium is complexed with the oomycetes chemical bactericide, such that the pesticide dosage is reduced, and controlling effect is improved. The bactericide is an environment-friendly bactericide. The bactericide is advantaged in high biological safety, low cost, and substantial economic benefit.

The invention discloses a harmless management method for diseases, insects and weeds at a tea tree seedling stage of an organic tea garden and belongs to the technical field of tea leaf ecological production. The method specifically comprises four steps of manually weeding, covering light reflecting weeding films, hanging yellow sticky boards and releasing preying acarus. The method is adopted for production management, chemical herbicides need not to be applied for weeding at the seedling stage of the organic tea garden, chemical pesticides are not used for insect prevention, the labor cost of weeding at the seedling stage can be greatly reduced, no pesticide residue exist, environment pollution does not exist, and the production of organic tea is really realized.

The invention discloses a three-dimensional composite duck and shrimp culture method carried out in a rice field. The method includes the following steps of preparing the rice field, selecting rice seeds, ducklings and shrimps, cultivating rice, putting the ducklings into the rice field, putting the shrimps into the rice field, carrying out field management, luring the ducklings to get out of the rice field, harvesting rice and catching the shrimps. The ecological adaptability of the method is high, the structure of products obtained in the method is various, the economic efficiency is high, diseases, insect pests and weeds can be prevented for the rice by means of the ducklings and the shrimps, the use quantity of pesticides and chemical fertilizers can be reduced, pollution to the environment is reduced and planting cost can be further reduced.

The invention relates to aquaculture bivalve offspring seed production, in particular to a method for preventing pinna pectinata larvae from floating upwards and adhering. Two wave-making pumps are arranged in a diagonal shape on the pond wall of a culture pond in a width direction; waves and water flows which are generated by the wave-making pumps are used for dispersing the pinna pectinata larvae so that the pinna pectinata larvae are uniformly distributed in the culture pond, thus the pinna pectinata larvae are prevented from floating upwards and adhering; 24 hours after pinna pectinata fertilized ovums are incubated into D-shaped larvae, when the D-shaped larvae float upwards and are adhered, the wave-making pumps are installed; the proper power of the wave-making pumps is 12-30 W, the proper flow rate is 5-10 m3 / h, the proper distance horizontally kept between the water outlet centers of the wave-making pumps and a water surface is 15-25 cm, and the proper distance between the water outlet centers of the wave-making pumps and the adjacent pond wall of the culture pond is 20-40 cm. The invention can reduce upward floating and adhesion ratio matched groups of the pinna pectinata larvae more than 80 percent, enhance the survival rate by 80 percent without obvious adverse effect on the growth of the larvae and achieve the culture density of the larvae by 4-5 / ml, thereby effectively enhancing the survival rate and the culture efficiency of the pinna pectinata larvae.

The invention provides a composite corn seed coating agent which comprises the following raw materials according to the mass percent: 4 percent to 7 percent of chlorpyrifos, 2 percent to 4 percent of dniconazole, 0.1 percent to 0.5 percent of compound sdium ntrophenolate, 1 percent to 1.5 percent of film-forming agent, 1 percent to 1.5 percent of dispersant, 2 percent to 3 percent of calcium dodecyl benzene sulfonate, 1 percent to 1.5 percent of preservative agent, 1 percent to 4 percent of antifreeze agent, 5 percent to 10 percent of bulking agent, 1 percent to 1.5 percent of alert color and the rest of water. The seed coating agent is mainly used for seed coating before the corn sowing, can effectively control the maize head smut and the soil insects, also can promote the breeding of roots and healthy seedlings, enhance the anti-adversity of the crops and improves the yield of the corns. The test result shows that the control effect of the seed coating agent to the maize head smut reaches 87.65 percent and 90.53 percent, and the control effect of the seed coating agent to the soil insects of the corns reaches 76.84 percent and 85.69 percent, and the yield increasing rate reaches 7.6-10.2 percent.

The invention discloses a hydroponic cultivation method of spinach comprising following steps: A accelerating germination; B. sowing; C. transplanting: single spinach seedlings are moved into nursery tanks of a nursery container, and are exposed under ultraviolet lamps for 1 hour and exposed under LED daylight lamps for 7 hours, and then cultivated in the dark for 16 hours every day, after 3 weeks, seedling culture liquid is replaced once until harvesting. The seedling culture liquid is prepared by following components: 1.2[mu]mol / L manganous sulfate, 0.2[mu] mol / L ammonium molybdate, 0.6[mu] mol / L cobalt chloride, 0.1 [mu]mol / L cobalt chloride nickel chloride, 0.1 mmol / L 5-aminolevulinic acid, 2 mmol / L ammonia sulfate and 5 to 10 g / L extracting solution; magnetic water is used as the solvent of the seedling culture liquid, and the pH is regulated to 6.5 to 7.0. By means of the hydroponic cultivation method of spinach, the yield of hydroponic spinach in winter can be increased, the content of antioxidant substance in the spinach is increased and therefore the health care effect of spinach is enhanced.

The invention discloses a method for preparing a PCR template rapidly in a large batch manner, belongs to the technology field of plant molecule detection, and in particular relates to thermal cracking of plant tissues in lysate in order to realize the neutralization with the lysate. The method adopts the technical proposal that trace plant leaves are put in the lysate with certain volume for thermal cracking and neutralization; reaction mixed solution can be used as the template of PCR reaction directly; the PCR reaction is stable and the repeatability is strong under the circumstance of adding with proper polymerase stabilizing agent. The method is particularly suitable for the rapid preparation of the PCR reaction template in the large batch manner, greatly increases the efficiency of the molecule detection and the genetic analysis, and lowers the cost extremely markedly.

The invention relates to a method for preparing ternary-component AlxGaI-xN nanocone, realizing control in the full-ingredient range (x is larger than 0 and smaller than 1). The method for preparing the ternary-component AlxGaI-xN nanocone comprises the following steps of: making anhydrous GaCl3 as a gallium source and anhydrous AlCl3 as an aluminum source with a nitrogen source by adopting a chemical vapor deposition method under the condition that the evaporation temperatures of GaCl3 and AlCl3 are respectively 70-90 DEG C and 130-150 DEG C to generate single-phase AlxGaI-xN nanocone, wherein x is larger than 0 and smaller than 1, the preferred nitrogen source is NH3, and the deposition temperature is 650-800 DEG C. In the invention, the vapor partial pressure of reaction precursors GaCl3 and AlCl3 is finely controlled in a tube furnace of a three-segment temperature area to obtain a one-dimensional nano material with a single-crystal structure and uniformly distributed components, and the atom ratio of Al / Ga can be controlled in the full-ingredient range (x is larger than 0 and smaller than 1) by changing growth conditions.

The invention relates to the technical field of water-based colour paste and provides water-based colour paste which is environmentally friendly, non-toxic, good in dispersing performance, strong in tinting strength and resistant to light, does not become thick, has anti-ultraviolet ray property, good weatherability and resistance to chemical attack and does not get mildew. The water-based colour paste is prepared from the following raw materials in parts by weight: 50-60 parts of pigment, 4-6 parts of wetting agent, 9-12 parts of dispersing agent, 6-10 parts of antioxidant, 0.3-0.45 part of antiseptic, 0.3-0.5 part of mildew preventive, 0.5-0.7 part of water-based defoaming agent, 6-8 parts of thickening and anti-settling agnet, 6-10 parts of ultraviolet light absorber and 30-60 parts of deionized water.

The invention discloses a cultivating device for agricultural planting. The culturing device for agricultural planting comprises a halogen lamp, a planting cultivating box, cultivating troughs and a base, wherein the planting cultivating box is mounted at the top of the base, the cultivating troughs are horizontally hinged to both sides of a rotary shaft in the planting cultivating box, and an electromagnetic guide rail is horizontally mounted on the part, directly above each cultivating trough, of the rotary shaft; a fixed rod is horizontally arranged at the bottom of each electromagnetic guide rail through a slide block, a water distributing pipe is laid in each fixed rod, and rotary sprayers are evenly arranged at the bottom of each water distributing pipe through a duct. According to the cultivating device for agricultural planting, through the arrangement of the slide blocks, the fixed rods, the water distributing pipes, the rotary sprayers, a fertilizer solution storage tank anda water pump, a fertilizer solution can be pumped into the water distributing pipes through the water pump at any time during seedling raising, fertilization is conducted on seedlings through the rotary sprayers, the operation is convenient, and by utilizing constant movement of the slide blocks on the electromagnetic guide rails, uniform fertilization applying on the seedlings can be achieved, sothat the situation of inconsistent growth of the seedlings is avoided.

The invention discloses a lactose induction type expression vector, which can utilize cheap lactose to replace expensive isopropyl thioamide-beta-D- galactoside (IPTG) to be exutory. The lactose induction type expression vector can be widely used on the expression of exogenous gene and the reconstruction of serratia marcescens metabolic pathway, for example, the lactose induction type expression vector is used to lead meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) in serratia marcescens. The invention also discloses a serratia marcescens which utilizes gene recombination technique to reconstruct, which changes the metabolic pathway of natural meso-2,3-butanediol in bacteria through leading in exogenesis meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) gene. The serratia marcescens which is reconstructed by gene recombination technique which is provided by the invention expresses meso-2,3-butanediol dehydrogenase in an expected level, which improves production and accumulation of meso-2,3-butanediol in host strain, and improves the productivity of meso-2,3-butanediol in serratia marcescens.

The invention relates to the field of agricultural equipment and provides an integrated solid-liquid fertilizer distributor stirring device and a method thereof. The integrated solid-liquid fertilizerdistributor stirring device comprises a base, a stirring tank is fixedly connected at the top of the base, a partition plate is fixedly and vertically connected to the inner center of the stirring tank and partitions the stirring tank into a liquid fertilizer cavity and a solid fertilizer cavity, and a stirring motor is arranged at the top, corresponding to the liquid fertilizer cavity, of the stirring tank. A driving end of the stirring motor penetrates the stirring tank to fixedly connect with a stirring rod. By arrangement of a scattering mechanism, caked fertilizers in solid fertilizers can be scattered to avoid blockage of a discharging port, and influences on crop growth due to excessive nutrients of lump fertilizers can be avoided. In addition, an electric push rod drives a brush to extend out to contact with the bottom, the liquid fertilizer cavity can be better cleaned, and functional diversity is improved.

The invention belongs to the technical field of cultivation of vegetables, flowers, rice, fruit trees, grapes, hops or edible seaweeds, and discloses a kiwifruit planting method. The method includes the steps of soil preparation, interplanting, seedling, scaffolding, shaping, fertilization, disease and pest prevention, pollination and the like, and by interplating euphorbia tirucalli linn, not only can the ground grip effect of kiwifruits be increased, but also disease and pest prevention effects are achieved. The method solves the problem in the prior art that fruits easily fall in windy dayswhen the kiwifruits have the fruits.

The invention discloses a culture method capable of reducing chlorella adherence.The culture method includes preparing a BG-11 culture solution, and adding a 10-300 ppm emulsifying agent into the BG-11 culture solution to obtain a culture solution capable of reducing chlorella adherence.The culture method capable of reducing chlorella adherence means that the culture solution capable of reducing chlorella adherence is used for culturing chlorella.When cultured chlorella cells reach a stable phase, dry cell weight as high as 875-956 mg can be obtained from every L of culture solution and is increased by 40-66% than a BG-11 chlorella culture solution without the emulsifying agent.The culture solution capable of reducing chlorella adherence has no adverse effect on growth of the chlorella, and accordingly the cultured chlorella is safe and free of toxicity and is particularly suitable as raw materials of health products, feed, food additives, fine chemical products and medical preparations.

The invention relates to a simple and convenient agrobacterium tumefaciens-mediated sugarcane transgene method. The transgene method comprises the steps of preparing a bacteriostatic agent, disinfecting a culture container, transforming agrobacterium tumefaciens, preparing a culture medium, co-culturing, selectively culturing, culturing by screening, culturing strong seedlings and culturing by rooting, wherein the bacteriostatic agent can be simply prepared; the culture medium can be prepared by preparing the required components according to different conventional methods of culture medium formulae and adding the bacteriostatic agent; both the culture container and the culture medium do not need to be sterilized by high temperature and high pressure, so that in a process of preparing the culture medium of the agrobacterium tumefaciens-mediated sugarcane transgene, the working amount and the energy consumption are greatly reduced, the tissue culture process is simplified, and the tissue culture cost is lowered; compared with the conventional agrobacterium tumefaciens-mediated sugarcane transgene method, the cost can be reduced by above 10%. The simple and convenient agrobacterium tumefaciens-mediated sugarcane transgene method is simple to operate, strong in practicality and good in popularity.