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Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide

A technology of Bacillus subtilis and fungicides, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of easy resistance of pathogenic bacteria, high resistance risk level, low toxicity of pathogenic bacteria, etc., and achieve synergistic effect Significant, reducing pesticide residues, increasing the effect of the site of action

Inactive Publication Date: 2012-10-24
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mancozeb is a protective fungicide, which has low toxicity to pathogenic bacteria in the prevention and treatment of downy mildew of Chinese cabbage. If it is to effectively prevent and control the disease, the dose required is high, and the problem of pesticide residues is serious; As a new type of systemic fungicide, phylloline has a single site of action, long-term use, pathogenic bacteria are prone to drug resistance, and it is also easy to form cross-resistance with other agents, and the risk of resistance is high

Method used

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  • Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide
  • Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide
  • Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Get 34.01 grams of 98% metalaxyl former powder, Bacillus subtilis former powder (1.05 × 10 12 pcs / g) 37.7 grams, 25 grams of sodium butyl naphthalene sulfonate, 50 grams of sodium naphthalene sulfonate formaldehyde condensate and 353.29 grams of light calcium carbonate. Grind the rice after mixing to make 6.67% metalaxyl 7.9×10 10 Live spores / gram Bacillus subtilis wettable powder 500 grams. Metalaxyl (metalaxyl) Nanjing Heyuan Chemical Co., Ltd.

Embodiment 2

[0042] Example 2 Take 15.31 grams of 98% propamocarb stock solution, Bacillus subtilis fermentation concentrate (1.0 × 10 11 pieces / g) 335.78 grams, emulsifier 0203B 25 grams and water 123.91 grams. First mix the propamocarb stock solution with the Bacillus subtilis fermentation concentrate, stir while mixing, then add 25 grams of 0203B, add water to make up 500 grams, and stir evenly to make 500 grams of 3% propamocarb·6.72×10 10 live spores per gram of Bacillus subtilis aqueous solution. Propamocarb (propamocarb) Zhejiang Heben Technology Co., Ltd.

[0043] 0203B Shandong Tiandao Biological Engineering Co., Ltd.

Embodiment 3

[0044] Example 3 Take 10.20 grams of 98% propamocarb stock solution, Bacillus subtilis fermentation concentrate (1.0 × 10 11 pieces / g) 335.42 grams, emulsifier 0203B 25 grams and water 129.38 grams. First mix propamocarb stock solution with Bacillus subtilis fermentation concentrate, stir while mixing, then add 25 grams of 0203B, add water to make up 500 grams, stir evenly, and then make 500 grams of 2% propamocarb·6.72×10 10 live spores per gram of Bacillus subtilis aqueous solution.

[0045] Propamocarb (propamocarb) Zhejiang Heben Technology Co., Ltd.

[0046] 0203B Shandong Tiandao Biological Engineering Co., Ltd.

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Abstract

The invention relates to a pesticide, and especially relates to a complex bactericide composed of bacillus subtilis and a chemical bactericide. The bactericide is used for controlling brassica rapa pekinensis downy mildew. The bactericide comprises the chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, and auxiliary materials. The bactericide is characterized in that the chemical bactericide in the active components is any one selected from metalaxyl, propamocarb, and mancozeb. A biocontrol bacterium is bacillus subtilis XM16, with the strain collection number of CGMCC No.0372. A weight ratio of metalaxyl to bacillus subtilis XM16 is 1-8:1-10. A weight ratio of propamocarb to bacillus subtilis XM16 is 1-10:15-140. A weight ratio of mancozeb to bacillus subtilis XM16 is 1-15:1-10. A form of the bactericide is usually hydrophilic powder or aqueous solution. The complex agent of the biocontrol bacterium and the chemical bactericide has a substantial synergetic effect in controlling brassica rapa pekinensis downy mildew. The biocontrol bacterium bacillus subtilis XM16 is advantaged in high bacteriostatic activity, high stress resistance, and wide bactericidal spectrum. The biocontrol bacterium is complexed with the oomycetes chemical bactericide, such that the pesticide dosage is reduced, and controlling effect is improved. The bactericide is an environment-friendly bactericide. The bactericide is advantaged in high biological safety, low cost, and substantial economic benefit.

Description

technical field [0001] The invention relates to a compound fungicide, which achieves the purpose of preventing and treating downy mildew of Chinese cabbage by compounding Bacillus subtilis and oomycete fungicide, belongs to the field of biological control and chemical control of plant diseases, and is specially used for the comprehensive control of downy mildew of Chinese cabbage . Background technique [0002] Chinese cabbage (Brassica rapa pekinensis) is a common cruciferous vegetable. Chinese cabbage downy mildew is an important oomycete disease caused by parasitic downy mildew (Peronospora parasitica). %Loss. In the 1960s, the Chinese cabbage downy mildew epidemic occurred, which caused serious losses to agricultural production. Because downy mildew seriously affects the yield and quality of Chinese cabbage, measures such as strengthening cultivation management, selecting disease-resistant varieties, and chemical agent control are usually adopted in production to reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N47/14A01N47/12A01N37/46A01N25/14A01N25/04A01P3/00
Inventor 梁晨杨丽娟李宝笃赵洪海辛玉成
Owner QINGDAO AGRI UNIV
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