2 results about "High level expression" patented technology

The present invention relates to an IFITM scaffold enabling high-efficiency engineering of extracellular vesicles. In the present invention, active substances to be expressed in extracellular vesicles are linked to the IFITM scaffold so that the active substances can be expressed in the vesicles at a high level. In particular, the active substance linked to the C-terminus of the IFITM scaffold is present on the surface of the vesicles to exhibit the intrinsic activity. In addition, the present invention provides a U-shaped IFITM scaffold, which enables two types of active substances linked to the N-terminus and the C-terminus of the IFITM scaffold to be present on the surface of extracellular vesicles at a 1:1 ratio.

This invention relates to an engineered *Escherichia coli* strain and a method for preparing gene 3 knockout helper phages. The genome of the engineered *E. coli* strain integrates a DNA module containing elements such as T7 Lysozyme, araC, ParaBAD, T7 RNA polymerase, PT7-lacO, and Ff gene 3. Through dual induction with L-arabinose and IPTG, tight regulation and high-level expression of the g3p protein can be achieved. Its property of strictly limiting leakage expression effectively reduces g3p protein degradation and misfolding. Using this strain to produce gene 3 knockout helper phages significantly improves the packaging efficiency and yield of the helper phages, reaching ≥ 8.5 × 10⁻⁶. 14 The pfu / L concentration is at least 600 times higher than existing technologies, significantly reducing production costs. This invention has significant application value in the field of phage display technology.