66 results about "Aconitic acid" patented technology

The invention provides bacillus thuringiensis for efficiently poisoning and killing plant parasitic nematodes and application of the bacillus thuringiensis, and belongs to the technical field of microorganisms. According to the invention, a strain of bacillus thuringiensis BMB-0181 capable of efficiently poisoning and killing plant parasitic nematodes is screened from soil, and the preservation number of the bacillus thuringiensis BMB-0181 is CCTCC (China Center for Type Culture Collection) NO: M 20242644. According to the present invention, the detection results of the strain fermentation broth prove that the trans-aconitic acid yield can be high, and the biological assay and the pot culture experiment prove that the strain has the high poisoning activity on the meloidogyne incognita, can effectively control the field planting of the meloidogyne incognita, and further has the certain growth promoting effect on the tomato; the strain disclosed by the invention can be developed into a novel biological nematode killing pesticide.

The invention relates to a carboxylic acid ligand-copper metal organic framework nano material, a preparation method thereof and an injection. The carboxylic acid ligand-copper metal organic framework nano material is of a polycrystal MOF structure formed by connecting carboxylic acid ligands and copper ions through coordinate bonds; the carboxylic acid ligand comprises at least one of dicarboxylic acid and tricarboxylic acid; preferably, the dicarboxylic acid comprises at least one of itaconic acid and fumaric acid, and the tricarboxylic acid comprises at least one of aconitic acid and citric acid; the valence states of the copper ions comprise zero valence, + 1 valence and + 2 valence; preferably, based on the total amount of the copper ions being 100 at%, the total content of Cu0 and Cu + is 85-90 at%, and the content of Cu2 + is 10-15 at%.

The invention provides a recombinant Aspergillus niger strain with high yield of trans-aconitic acid, and belongs to the technical field of gene engineering. The recombinant aspergillus niger strain can simultaneously express aconitase and aconitic acid isomerase. According to the genetic engineering aspergillus niger strain provided by the invention, the trans-aconitic acid fermentation yield is generally higher than that of an original strain, and the strain with the highest yield can reach 51g / L on a shake flask level, so that the genetic engineering aspergillus niger strain has very high application value.

The invention relates to a high-yield trans-aconitic acid engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene engineering. In the scheme of the invention, the inventor introduces mitochondrial localization signal peptide to be combined with the aconitic acid isomerase to form a gene element, and an expression cassette containing the gene element is introduced into the strain by adopting a genetic engineering technical means, so that the aconitic acid isomerase expressed in the constructed recombinant strain can be localized into mitochondria, and the expression efficiency of the recombinant strain is improved. Metabolic pathways related to trans-aconitic acid synthesis are located in the same zone, so that the capacity of fermenting and producing trans-aconitic acid by the recombinant strain is improved. The technical scheme disclosed by the invention not only has a good application prospect and production economic value, but also provides a referential new thought for transformation of strains related to other fermentation processes.

The application provides a recombinant Aspergillus niger with high trans-aconitic acid yield and a construction method and application thereof, and relates to the technical field of genetic engineering. Specifically, the recombinant Aspergillus niger heterogeneously expresses aconitate isomerase TbrA and / or Adi1. The application provides a new engineering strain for efficient production of trans-aconitic acid, solves the source predicament of needing plant extraction or needing chemical synthesis in traditional production of trans-aconitic acid, and greatly improves the yield of trans-aconitic acid.

The invention provides a composite surfactant for oil displacement of a hypersalinity oil reservoir and a preparation method of the composite surfactant, and relates to the technical field of petrochemical engineering. The composite surfactant is prepared from the following components in percentage by weight: 0.1 to 0.3 percent of anionic-nonionic surfactant, 0.01 to 0.03 percent of cationic surfactant and the balance of formation water, wherein the anionic-nonionic surfactant is prepared by the following steps: reacting a hydroxyl-containing compound with ethylene oxide to obtain polyoxyethylene ether, then carrying out esterification modification by using aconitic acid modified rosin, then sulfonating by using a sulfonating agent, and finally neutralizing. The composite surfactant shows relatively low interfacial tension under the condition of hypersalinity (50000-200000 mg / L), is high in stability, and can be used for improving the recovery efficiency of hypersalinity oil reservoirs.

The invention belongs to the technical field of water treatment, and particularly discloses a green scale inhibitor for barium sulfate and a preparation method thereof, the scale inhibitor is a product generated by esterification reaction of polysuccinimide and hydroxyethyl methylacrylate under alkaline conditions, and a copolymer formed by graft copolymerization of aconitic acid and sodium lignin sulfonate, wherein the mass ratio of polysuccinimide to hydroxyethyl methylacrylate to aconitic acid to sodium lignin sulfonate is 2: 4: (1-6): (1-4). The scale inhibitor is a composite copolymer formed by polymerizing and grafting a product obtained after esterification of polysuccinimide / hydroxyethyl methylacrylate, aconitic acid and sodium lignin sulfonate, has carboxyl, hydroxyl, amino, sulfonic acid and other groups at the same time, and can generate strong interaction with key ions or sites in the BaSO4 crystal growth process, so that the scale inhibitor can be used for inhibiting the growth of BaSO4 crystals. The normal growth and aggregation of BaSO4 crystals are prevented, so that the formation of BaSO4 scales is effectively inhibited.

The invention discloses an electrolyte additive and application thereof, and particularly relates to application in an aqueous zinc battery. The electrolyte additive is trans-aconitic acid (TAA), the trans-aconitic acid and electrolyte salt are added into water to form an electrolyte, and the electrolyte is applied to the aqueous zinc battery. The trans-aconitic acid is used as an electrolyte additive, so that the electrolyte is safe and environment-friendly; the trans-aconitic acid molecule contains three carboxylic acid groups and one unsaturated double bond, has strong coordination ability with metal ions, can effectively regulate and control the solvation structure of the metal ions, and can participate in formation of a stable solid electrolyte interface, inhibition of side reaction and the like; the electrolyte containing the trans-aconitic acid as the additive is applied to the water-based zinc battery and has remarkable effects on hydrogen evolution reaction, corrosion inhibition, zinc dendrite growth and the like of the battery, and the water-based zinc battery with super-long cycle life is obtained.

The invention provides recombinant aspergillus niger capable of producing trans-aconitic acid at high yield as well as a construction method and application of the recombinant aspergillus niger, and relates to the technical field of genetic engineering. Specifically, the recombinant aspergillus niger is used for heterologous expression of aconitic acid isomerase TbrA and / or Adi1. A new engineering strain is provided for efficient production of trans-aconitic acid, the source dilemma that plant extraction or chemical synthesis is needed in traditional production of trans-aconitic acid is solved, and the yield of trans-aconitic acid is greatly increased.

The invention belongs to the field of high polymer materials, and particularly relates to a biodegradable composite material and a preparation method thereof. The technical problem that an existing biodegradable material product is poor in aging resistance and barrier property is solved. Aromatic-aliphatic copolyester with a side chain is used as a base material, and an esterification-polycondensation process is performed to prepare the polyester. The composite material comprises the following components in parts by weight: 60-90 parts of a copolyester material, 10-30 parts of other blended resin, 20-40 parts of an inorganic filler, 0.5-2 parts of a lubricant and 0.01-2 parts of an epoxy chain extender. The epoxy chain extender is an epoxy chain extender with bio-based aconitic acid as a framework, and the mechanical property and the anti-aging property of the degradable composite material are comprehensively improved based on coordination and cooperation of the base material, the epoxy chain extender and other raw materials. The composite material is expected to be applied to the fields of biodegradable shopping bags, agricultural plastic mulching films, foaming materials and the like.

The invention discloses application of a biomarker in diagnosis of lung cancer soft meningeal metastasis. According to the present invention, it is found for the first time that the metabolites such as Indolastic acid, L-Glutic acid, N-Lactoylphenylalanine, cis-Acetic acid, Formiglumic acid and N-Acetylneomic acid in the cerebrospinal fluid have good diagnosis efficiency on the lung cancer soft meningeal metastasis, have high accuracy, high sensitivity and high specificity, can be used as the biological marker of the lung cancer soft meningeal metastasis, and can be used in the effective diagnosis of the lung cancer soft meningeal metastasis. The invention provides a brand-new thought and strategy for research and development of related diagnostic products for the lung cancer soft meningeal metastasis, and has wide application prospects and important transformation significance in the technical field of diagnosis of the lung cancer soft meningeal metastasis.

The present invention provides a strain of Bacillus thuringiensis that is highly effective in killing plant parasitic nematodes and its application, belonging to the field of microbial technology. The present invention screens and obtains a strain of Bacillus thuringiensis (Bacillus thuringiensis) BMB-0181 that is highly effective in killing plant parasitic nematodes from soil, and the deposit number is CCTCC NO:M 20242644. The fermentation liquid of the strain is used to detect and confirm that it can produce high trans-aconitic acid. It is confirmed by bioassay and potted plant experiments that it has high toxicity to southern root-knot nematodes, can effectively control the colonization of southern root-knot nematodes, and has a certain growth-promoting effect on tomatoes. The strain of the present invention can be developed into a new biological nematode pesticide.

The invention relates to the technical field of concrete admixtures, and particularly discloses a rapid dispersing type polycarboxylate superplasticizer and a preparation method thereof.The rapid dispersing type polycarboxylate superplasticizer is prepared from, by mass, 200-300 parts of polyether monomer, 1.5-3.5 parts of oxidizing agent, 0.2-0.5 part of reducing agent, 1.2-3 parts of chain transfer agent, 25-33 parts of unsaturated acid, 10-20 parts of self-made functional monomer modified alkyl glycoside, 5-10 parts of aconitic acid and 250-300 parts of tap water. The preparation method comprises the following steps: dissolving, preparing a solution A, preparing a solution B and synthesizing to prepare the quick dispersing type polycarboxylic acid water reducing agent. By introducing alkyl glycoside, wetting of cement particles is promoted, water-cement interfacial tension is rapidly reduced, meanwhile, cement particle aggregation is hindered through stereoisomerization of alkyl glycoside, the effect of rapid dispersion of cement particles is achieved, and modified alkyl glycoside provides adsorption points and electrostatic repulsion of cement particles to hinder cement particle aggregation, so that the cement particles are dispersed rapidly. The cement particles can be quickly dispersed in a low-temperature environment.

The application discloses a terpolymer scale inhibitor and a preparation method thereof, and belongs to the technical field of polymer macromolecules and water treatment. The terpolymer scale inhibitor has the following general structure formula: wherein x, y and n are any integer in 1-10. In addition, the application further provides a preparation method of the terpolymer scale inhibitor, which comprises the following steps: mixing aconitic acid and water, stirring and dissolving at 50-55 DEG C, then adding N,N-dimethyl acrylamide and p-aminobenzoic acid, then increasing the temperature to 65-85 DEG C and adding an initiator dropwise, and then reacting at 65-90 DEG C to obtain the terpolymer scale inhibitor. The scale inhibitor is green and environment-friendly, and has excellent scale inhibition performance.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for evaluating the prognosis of patients with hypertrophic cardiomyopathy and application thereof. The application determines that one or more of 3-hydroxyrilotene, asparagine-lysine, cyclohexylamine, arachidonic acid, aconitic acid, propionyl carnitine, ceramide, tryptophan, 3-hydroxysebacic acid, traumatic acid, phosphatidylcholine, alanine-proline, lysophosphatidylcholine, taurine and S-garlicin cysteine are related to the prognosis of hypertrophic cardiomyopathy, can evaluate the prognosis of patients with hypertrophic cardiomyopathy after undergoing muscle resection of interventricular septum, and shows accurate judgment on all-cause death or rehospitalization, cardiovascular events and postoperative new-onset arrhythmia. Finally, all the prognostic biomarkers are reproduced in an independent cohort, which is helpful for postoperative risk evaluation of patients with hypertrophic cardiomyopathy, has high specificity and high sensitivity.

The invention relates to the technical field of gene engineering, and discloses a recombinant strain, a construction method and application thereof, and a method for producing total fatty acid. The recombinant strain is obtained by genetic modification of an original strain, and compared with the original strain, the recombinant strain overexpresses citric acid transporter Yhm2p, cis-aconitase E.HA and isocitrate dehydrogenase IDP located in cytoplasm at the same time, and does not express isocitrate dehydrogenase IDH1 and isocitrate dehydrogenase IDH2 located in mitochondria. The recombinant strain provided by the invention forms a high-efficiency pathway of citric acid retention-cytoplasm transport-directional production of NADPH, so that the core bottleneck of insufficient supply of cytoplasm NADPH in fatty acid synthesis is solved, the total fatty acid yield is remarkably increased, the transformation logic is accurate, and a stable and reliable source is provided for industrial high-efficiency production of fatty acid.

The invention provides an application of metabolites in sow vaginal secretion in early pregnancy diagnosis, and finds that three metabolic molecules can be used as markers for the early pregnancy diagnosis of sows for the first time on the basis of non-targeted metabonomics and machine learning conjoint analysis. According to the present invention, the metabolic markers are respectively INDOLEPROPIONIC ACID (indolepropionic acid), cis-Amonitate (cis-Amonitate) and 4-p-Coumaroylquinic acid (4-p-coumaroylquinic acid), the ROC curve AUC values of the metabolic markers are respectively 0.88, 0.92 and 0.77, and the metabolic markers have excellent diagnosis performance during the combination diagnosis. The pregnancy diagnosis is carried out by adopting the vaginal secretions of the sows 18 days after hybridization, the effective pregnancy diagnosis time is advanced by 5-8 days on the basis of a wave diagnosis method, and the method has important significance for reducing cost and increasing ultrasonic efficiency in live pig feeding.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The invention discloses a high-toughness composite PVC film and a preparation method thereof. The high-toughness composite PVC film is prepared from the following components in parts by weight: 62 to 74 parts of PVC resin, 54 to 67 parts of oxidized polyethlene wax, 25 to 35 parts of silicon dioxide, 26 to 39 parts of aconitic acid monoester, 14 to 28 parts of terephthalic acid, 24 to 36 parts of silane coupling agent, 16 to 28 parts of ethylene propylene diene monomer, 14 to 18 parts of glyceryl stearate, 14 to 19 parts of rare earth stearic acid and 16 to 26 parts of oleamide. Compared with a film at the present stage, the PVC film provided by the invention forms a more stable spatial network structure, can further increase the air permeability, can effectively play a supporting effect, has higher toughness, and is mainly embodied in high elongation at break, high falling standard impact strength, improved impact strength and improved air permeability of the film.

The present invention belongs to the technical field of specialty paper preparation, and specifically relates to a method for producing specialty paper. The method for producing specialty paper of the present invention uses a compound of bleached sulfate masson pine pulp and bleached wheat straw pulp to construct a three-dimensional skeleton structure in which long and short fibers are interwoven, and uses modified calcium titanate whiskers, modified magnesium borate whiskers and polyacrylate microspheres as fillers. The rigid skeleton of the modified calcium titanate whiskers and the flexible buffer of the modified magnesium borate whiskers form a stress gradient transmission, and the addition of polyacrylate microspheres can reduce local stress concentration. The three act synergistically to improve tensile strength and folding endurance. The high retention rate of acorn starch ensures that the filler is in full contact with the fiber, providing dense reaction sites for trans-aconitic acid crosslinking, thereby forming a water-resistant crosslinked network. Thus, the raw materials work synergistically to ensure that the produced specialty paper has excellent dry tensile strength, bursting resistance, folding endurance and wet strength retention rate.

The invention belongs to the field of antioxidants, and particularly relates to a chitosan-based antioxidant as well as a preparation method and application thereof. The chitosan-based antioxidant provided by the invention is prepared by carrying out Michael addition reaction on an antioxidant with a structure as shown in a formula (I) and a biomass raw material and then carrying out amidation reaction with chitosan, the biomass raw material is one or more of itaconic acid, fumaric acid, mesaconic acid, muconic acid, aconitic acid, methacrylic acid, linoleic acid and terpenoids. According to the invention, a specific biomass raw material is taken as a bridging agent, and a small molecular antioxidant is grafted into chitosan through Michael addition reaction and amidation reaction, so that the molecular weight of the antioxidant is increased, and the thermal stability and migration resistance of the antioxidant are improved; meanwhile, a chitosan structure exists in the antioxidant, residual metal ions in the rubber emulsion can be chelated, the catalytic oxidation effect of the metal ions is inhibited, and the oxidation resistance of the antioxidant is improved.

The invention discloses a Trichoderma reesei engineering strain for producing trans-aconitic acid as well as a construction method and application of the Trichoderma reesei engineering strain, and relates to the technical field of biological engineering. The construction method comprises the following steps: transforming a coding gene of mitochondrial tricarboxylic acid transport protein and a coding gene of aconitic acid isomerase into a trichoderma reesei host bacterium, and carrying out gene recombination construction to obtain the trichoderma reesei engineering strain, the amino acid sequence of the mitochondrial tricarboxylic acid transporter is as shown in SEQ ID NO. 1; the amino acid sequence of the aconitic acid isomerase is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. A new production strain is provided for microbial fermentation production of trans-aconitic acid, a large amount of trans-aconitic acid can be produced through direct fermentation by using common carbon sources such as glucose or cellulose, and the highest yield can reach 26.5 g / L.

The invention provides a high-density drilling fluid with high temperature resistance and a preparation method thereof, and the high-density drilling fluid comprises the following components in parts by weight: 100 parts of water; 0.5 to 4 parts of a clay substance; 1-6 parts of a non-differentiated functional long-acting treatment agent; 1-6 parts of a humic acid auxiliary material; 1-4 parts of a pH value regulator; 0 to 15 parts of salt; 200 to 250 parts of a weighting agent; the non-differentiated function long-acting treating agent is prepared by carrying out polymerization reaction on an esterified aconitic acid monomer, an itaconic acid monomer, an acrylic acid monomer and a 2-acrylamido-2-methylpropanesulfonic acid monomer according to a certain mass ratio. The high-density drilling fluid is simple in composition and preparation, the rheological property and high-temperature and high-pressure filter loss of the drilling fluid are easy to control, the long-term high-temperature stability is greatly improved, the high-density drilling fluid can be applied to drilling construction of high-temperature and high-pressure strata, the components and dosage of a treating agent are greatly reduced, and the long-term high-temperature stability of the high-temperature and high-density drilling fluid is improved; and well drilling construction is facilitated.

Provided is a novel cis-aconitate synthesis enzyme, more particularly, a recombinant microorganism for producing itaconate including a cis-aconitate synthesis enzyme variant. According to the present disclosure, it was confirmed that the production and yield of itaconate were significantly increased in the recombinant microorganism for producing itaconate into which the novel cis-aconitate synthesis enzyme was introduced. In addition, it was confirmed that in the recombinant microorganism for producing itaconate of the present disclosure, a new carbon flow to itaconate was separated from the existing TCA cycle based on the activity of the corresponding enzyme. Accordingly, the novel aconitate synthesis enzyme of the present disclosure and the recombinant microorganism introduced with the aconitate synthesis enzyme can increase the economic feasibility of itaconate, and thus can be used in various industrial fields, such as synthetic resins, latexes, and food additives in which itaconate is used.

The invention discloses an enzymatic synthesis process of isocitric acid, belonging to the field of drug intermediate enzyme catalysis. Comprise the following steps: step one, the conversion of isocitric acid, in the reaction system, citric acid, water and aconitase solution are respectively added to form a reaction mixture, water bath isothermal reaction, and isocitric acid reaction solution is obtained; during the reaction process, aconitase catalyzes citric acid into isocitric acid; step two, the purification of isocitric acid, the isocitric acid reaction solution is heated and activated carbon is added to adsorb impurities, and the clear liquid is filtered to obtain the clear liquid, and the clear liquid continuously rinses the ion exchange resin column, and the ion exchange resin column is washed after the rinsing is completed, and the ion exchange resin column is de-washed after the washing is completed, and the eluent is cooled and crystallized, and the potassium isocitrate finished product is obtained after solid-liquid separation and drying. The present invention is a kind of synthesis process that is convenient for preparing isocitric acid and prepares a high purity product.

The application provides a recombinant Aspergillus niger strain with high trans-aconitic acid yield, and belongs to the technical field of genetic engineering. The recombinant Aspergillus niger strain can simultaneously express aconitase and aconitate isomerase. The genetically engineered Aspergillus niger strain provided by the application has a generally higher trans-aconitic acid yield than a starting strain, and the strain with the highest yield can reach 51 g / L at a shake flask level, and has a very strong application value.

The invention relates to environment-friendly artificial leather as well as a preparation method and application thereof, and belongs to the field of high polymer materials. The artificial leather structure provided by the invention comprises a base cloth layer, a foaming layer and a surface layer which are sequentially laminated from inside to outside, the foaming layer is prepared from the following components in parts by weight: 100 parts of PVC (Polyvinyl Chloride) resin, 60 to 130 parts of aconite, 100 to 200 parts of filler, 3.5 to 8.0 parts of stabilizer and 8 to 16 parts of foaming agent; the surface layer is prepared from the following components in parts by weight: 65 to 75 parts of PVC (Polyvinyl Chloride) resin, 70 to 80 parts of aconite, 20 to 30 parts of filler and 1.5 to 4 parts of stabilizer. The artificial leather provided by the invention has better environmental protection property, elasticity and aging resistance, and the used aconite plasticizer has lower molecular weight and stronger molecular chain activity, and can effectively open chain entanglement of high-polymerization-degree PVC (Polyvinyl Chloride), thereby finally obtaining a high-elasticity PVC artificial leather product.

The invention relates to electronic atomization liquid and an electronic atomization device. The electronic atomized liquid is prepared from the following components in parts by mass: 75 to 99.997 parts of a solvent, 0.002 to 15 parts of a sweetening agent and 0.001 to 10 parts of an acidulant, wherein the total mass part of the electronic atomized liquid is 100 parts; wherein the acidulant is prepared from glycyrrhizic acid, aconitic acid, gluconic acid and 2-methyl-2-pentenoic acid. The electronic atomized liquid can have proper sweetness after being atomized, and the problems of charring and taste deformation in the atomization process are solved.

The application belongs to the technical field of rice cultivation and relates to a plant factory cultivation method for shortening the growth period of rice based on carbon and nitrogen nutrition regulation. In view of the technical problem that the existing plant factory rice cultivation method has a long growth period and is difficult to meet the rapid cultivation demand, the method comprises seed soaking, germination, seedling raising, post-transplanting management and harvesting, and the core is to transplant rice seedlings to a plant factory water culture system, and synchronous control of cultivation conditions is used to promote early flowering: maintaining CO2 concentration of 400-1600 ppm, using full-spectrum artificial light source, using basic nutrient solution with total nitrogen of 1.2-3.2 mM, molar ratio of ammonium nitrogen to nitrate nitrogen of 2:1-4:1 and pH of 5.5-6.5, and adding L-carnitine and aconitic acid metabolism promoters with a molar ratio of 1.2:1-2.2:1 at the tillering peak stage. The method is mainly used for rapid cultivation of rice in a plant factory, and the cultivation efficiency is improved by shortening the growth period.

The invention discloses a construction method of escherichia coli for efficiently synthesizing glycollic acid, and belongs to the technical field of biochemical engineering. According to the invention, citric acid synthase gltA and aconitic acid synthase acnA and acnB are overexpressed in escherichia coli cells, pyruvate dehydrogenase transcription inhibition factor pdhR is knocked out, transhydrogenase pntA and pntB are overexpressed, and transhydrogenase stA is knocked out, so that the constructed recombinant escherichia coli can produce glycollic acid by using glucose in a short time. The reaction cost is lower, the yield is higher, the concentration of glycollic acid produced after fermentation in a 5L fermentation tank for 48 hours is 81 g / L, the conversion rate is 0.62 g / g, and the production intensity is 1.69 g / L / h.