17 results about "Aconitic acid" patented technology

The application provides a recombinant Aspergillus niger with high trans-aconitic acid yield and a construction method and application thereof, and relates to the technical field of genetic engineering. Specifically, the recombinant Aspergillus niger heterogeneously expresses aconitate isomerase TbrA and / or Adi1. The application provides a new engineering strain for efficient production of trans-aconitic acid, solves the source predicament of needing plant extraction or needing chemical synthesis in traditional production of trans-aconitic acid, and greatly improves the yield of trans-aconitic acid.

The invention discloses application of a biomarker in diagnosis of lung cancer soft meningeal metastasis. According to the present invention, it is found for the first time that the metabolites such as Indolastic acid, L-Glutic acid, N-Lactoylphenylalanine, cis-Acetic acid, Formiglumic acid and N-Acetylneomic acid in the cerebrospinal fluid have good diagnosis efficiency on the lung cancer soft meningeal metastasis, have high accuracy, high sensitivity and high specificity, can be used as the biological marker of the lung cancer soft meningeal metastasis, and can be used in the effective diagnosis of the lung cancer soft meningeal metastasis. The invention provides a brand-new thought and strategy for research and development of related diagnostic products for the lung cancer soft meningeal metastasis, and has wide application prospects and important transformation significance in the technical field of diagnosis of the lung cancer soft meningeal metastasis.

The invention relates to the technical field of concrete admixtures, and particularly discloses a rapid dispersing type polycarboxylate superplasticizer and a preparation method thereof.The rapid dispersing type polycarboxylate superplasticizer is prepared from, by mass, 200-300 parts of polyether monomer, 1.5-3.5 parts of oxidizing agent, 0.2-0.5 part of reducing agent, 1.2-3 parts of chain transfer agent, 25-33 parts of unsaturated acid, 10-20 parts of self-made functional monomer modified alkyl glycoside, 5-10 parts of aconitic acid and 250-300 parts of tap water. The preparation method comprises the following steps: dissolving, preparing a solution A, preparing a solution B and synthesizing to prepare the quick dispersing type polycarboxylic acid water reducing agent. By introducing alkyl glycoside, wetting of cement particles is promoted, water-cement interfacial tension is rapidly reduced, meanwhile, cement particle aggregation is hindered through stereoisomerization of alkyl glycoside, the effect of rapid dispersion of cement particles is achieved, and modified alkyl glycoside provides adsorption points and electrostatic repulsion of cement particles to hinder cement particle aggregation, so that the cement particles are dispersed rapidly. The cement particles can be quickly dispersed in a low-temperature environment.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for evaluating the prognosis of patients with hypertrophic cardiomyopathy and application thereof. The application determines that one or more of 3-hydroxyrilotene, asparagine-lysine, cyclohexylamine, arachidonic acid, aconitic acid, propionyl carnitine, ceramide, tryptophan, 3-hydroxysebacic acid, traumatic acid, phosphatidylcholine, alanine-proline, lysophosphatidylcholine, taurine and S-garlicin cysteine are related to the prognosis of hypertrophic cardiomyopathy, can evaluate the prognosis of patients with hypertrophic cardiomyopathy after undergoing muscle resection of interventricular septum, and shows accurate judgment on all-cause death or rehospitalization, cardiovascular events and postoperative new-onset arrhythmia. Finally, all the prognostic biomarkers are reproduced in an independent cohort, which is helpful for postoperative risk evaluation of patients with hypertrophic cardiomyopathy, has high specificity and high sensitivity.

The invention relates to the technical field of gene engineering, and discloses a recombinant strain, a construction method and application thereof, and a method for producing total fatty acid. The recombinant strain is obtained by genetic modification of an original strain, and compared with the original strain, the recombinant strain overexpresses citric acid transporter Yhm2p, cis-aconitase E.HA and isocitrate dehydrogenase IDP located in cytoplasm at the same time, and does not express isocitrate dehydrogenase IDH1 and isocitrate dehydrogenase IDH2 located in mitochondria. The recombinant strain provided by the invention forms a high-efficiency pathway of citric acid retention-cytoplasm transport-directional production of NADPH, so that the core bottleneck of insufficient supply of cytoplasm NADPH in fatty acid synthesis is solved, the total fatty acid yield is remarkably increased, the transformation logic is accurate, and a stable and reliable source is provided for industrial high-efficiency production of fatty acid.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The application provides a recombinant Aspergillus niger strain with high trans-aconitic acid yield, and belongs to the technical field of genetic engineering. The recombinant Aspergillus niger strain can simultaneously express aconitase and aconitate isomerase. The genetically engineered Aspergillus niger strain provided by the application has a generally higher trans-aconitic acid yield than a starting strain, and the strain with the highest yield can reach 51 g / L at a shake flask level, and has a very strong application value.

The application belongs to the technical field of rice cultivation and relates to a plant factory cultivation method for shortening the growth period of rice based on carbon and nitrogen nutrition regulation. In view of the technical problem that the existing plant factory rice cultivation method has a long growth period and is difficult to meet the rapid cultivation demand, the method comprises seed soaking, germination, seedling raising, post-transplanting management and harvesting, and the core is to transplant rice seedlings to a plant factory water culture system, and synchronous control of cultivation conditions is used to promote early flowering: maintaining CO2 concentration of 400-1600 ppm, using full-spectrum artificial light source, using basic nutrient solution with total nitrogen of 1.2-3.2 mM, molar ratio of ammonium nitrogen to nitrate nitrogen of 2:1-4:1 and pH of 5.5-6.5, and adding L-carnitine and aconitic acid metabolism promoters with a molar ratio of 1.2:1-2.2:1 at the tillering peak stage. The method is mainly used for rapid cultivation of rice in a plant factory, and the cultivation efficiency is improved by shortening the growth period.

The invention discloses a construction method of escherichia coli for efficiently synthesizing glycollic acid, and belongs to the technical field of biochemical engineering. According to the invention, citric acid synthase gltA and aconitic acid synthase acnA and acnB are overexpressed in escherichia coli cells, pyruvate dehydrogenase transcription inhibition factor pdhR is knocked out, transhydrogenase pntA and pntB are overexpressed, and transhydrogenase stA is knocked out, so that the constructed recombinant escherichia coli can produce glycollic acid by using glucose in a short time. The reaction cost is lower, the yield is higher, the concentration of glycollic acid produced after fermentation in a 5L fermentation tank for 48 hours is 81 g / L, the conversion rate is 0.62 g / g, and the production intensity is 1.69 g / L / h.

The invention discloses a bio-based electrolyte additive, a preparation method and application, the bio-based electrolyte additive is N-(3-indoleethyl)-5-oxopyrrolidine-2, 3-dicarboxylic acid, the raw material of the additive is derived from renewable biomass resources, a tryptophan precursor can be derived from microbial metabolism of natural tryptophan, and the tryptophan precursor can be derived from natural tryptophan. The trans-aconitic acid can be directly extracted from plants such as aconite, and the N-(3-indoleethyl)-5-oxopyrrolidine-2, 3-dicarboxylic acid is synthesized through two green and renewable bio-based raw materials. As an electrolyte additive of a water-based zinc battery, the tryptamine-trans-aconitic acid composite electrolyte fuses interface adsorption of tryptamine and ion coordination capability of trans-aconitic acid, synergistically constructs a stable electrode-electrolyte interface, can synergistically inhibit side reactions such as growth of zinc dendrites and hydrogen evolution, significantly alleviates zinc negative electrode corrosion, realizes a long-cycle-life water-based zinc battery, and has a wide application prospect. The cycle life of the aqueous zinc battery reaches 2500 hours.

The invention discloses a recombinant yarrowia lipolytica strain with high yield of itaconic acid and a construction method and application thereof, and belongs to the field of strain cultivation, the recombinant yarrowia lipolytica strain is obtained by metabolism reconstruction of a wild type yarrowia lipolytica strain, a cis-aconitic acid decarboxylase gene cadA derived from Aspergillus terreus ATCC20542 is inserted into a genome, and a stable expression cassette is formed through a TEF1 promoter and a CYC1 terminator; a CIT1 gene front-end signal peptide is fused at the N end of the cadA to realize directional expression of mitochondria; iDH1, ACL1 and ADH1 genes are knocked out at the same time; cIT1 and ACO1 genes are overexpressed to enhance the flux of citric acid in the direction of cis-aconitic acid; and the noxE gene of Lactococcus lactis is introduced to regulate the balance of NADH (Nicotinamide Adenine Dinucleotide Hormone) in the cell. The strain is fermented for 72 hours under the conditions that glucose is used as a carbon source, the temperature is 30 DEG C and the pH value is 5.5-6.0, and the yield of itaconic acid can reach 12 g / L or above. According to the construction method, gene knockout and homologous recombination integration are realized by adopting CRISPR-Cas9, and a target strain is obtained after screening and verification. The method is used for preparing degradable polymers, plasticizers and medical intermediates. The method has the beneficial effects that the itaconic acid yield and metabolism efficiency are improved.

The application provides a gastric juice metabolite composition and application for early gastric cancer screening, a method for screening early gastric cancer related metabolites and an early gastric cancer disease risk prediction method, and provides a gastric juice metabolite composition for gastric cancer screening, which is composed of glutamic acid, cis-aconitic acid, methionine and taurine.The application can realize non-invasive sampling independent of gastroscopy based on the sampling capsule mode, and finally provides an effective non-invasive screening method for early gastric cancer for the clinic.Through non-invasive gastric juice acquisition and marker detection, non-invasive auxiliary diagnosis of gastric cancer can be realized, the compliance of the subject is improved, and the body damage is reduced.

The invention discloses application of biological energy active molecules in preparation of medical instruments for skin, and belongs to the technical field of medical instruments. In the application, the biological energy active molecules comprise at least one of succinic acid, alpha-ketoglutaric acid, malic acid, citric acid, cis-aconitic acid and fumaric acid. Biological energy active molecules are applied to preparation of hydrogel medical instruments for skin wound healing and / or scar inhibition, and are combined with natural polymer biological materials, so that on one hand, the obtained hydrogel has excellent biocompatibility, mechanical properties, air permeability, water absorption and storage stability; on the other hand, the hydrogel dressing also has multiple effects of supplying energy, inhibiting oxidative stress, regulating and controlling an inflammation microenvironment and the like, rapid wound healing is realized, wound scar formation is reduced, and the problems that an existing hydrogel dressing for the skin is relatively single in performance and function and difficult to meet the rapid wound healing requirement in a complex pathological environment are solved; wide application prospects are realized.

The application provides a CO2 capture method and a CO2 capture device coupled with electrochemical regeneration of hydrogen. The method comprises the following steps: absorbing CO2 in a CO2-containing gas by using an alkaline solution to form a CO2-rich solution; performing acid-base neutralization of the CO2-rich solution and an acidic solution to obtain a neutralization solution and CO2; performing electrolysis of the neutralization solution to obtain a cathode electrolysis product and an anode electrolysis product, wherein the cathode electrolysis product is H2 and the alkaline solution, and the anode electrolysis product is O2 and the acidic solution; and the acidic solution is selected from one or a combination of more than two of the following solutions: pyruvic acid solution, gluconic acid solution, malonic acid solution, tartaric acid solution, glyceric acid solution, oxalacetic acid solution, formic acid solution, isopropionic acid solution, isobutyric acid solution, fumaric acid solution, aconitic acid solution, isocitric acid solution and hexanediol diacid solution. The acidic solution generated by electrolysis can avoid corrosion of the device equipment and reduce the net energy consumption and net cost of the CO2 capture process.

To provide a tree trunk injection agent having good stability of an active ingredient.SOLUTION: A tree injection containing an aqueous alcohol solution soluble compound such as acetic acid, hydroxyacetic acid, methacrylic acid, acrylic acid, oxalic acid, malonic acid, succinic acid, adipic acid, tartaric acid, diethyl tartrate, trans-aconitic acid, citric acid, gallic acid, phthalic acid, isophthalic acid, terephthalic acid, butanediol, trimethylolpropane, glycerol, dithiothreitol, dithioerythritol, sorbitol, maltitol, and reduced starch syrup, a monohydric alcohol having 1 to 4 carbon atoms such as methanol, ethanol, 1-propanol, 2-propanol, and 1-butanol, acetamiprid, and water.SELECTED DRAWING: None

This invention provides a recombinant *Yarrowia lipolytica* strain, its construction method, and a method for preparing trans-aconitic acid. The provided recombinant *Yarrowia lipolytica* carries the following nucleotide fragments: a nucleotide fragment encoding aconitic acid isomerase and a nucleotide fragment encoding a mitochondrial tricarboxylic acid transporter. *Yarrowia lipolytica* is a biosafe strain, and the recombinant *Yarrowia lipolytica* strain constructed using it can synthesize trans-aconitic acid using common carbon sources such as glucose and glycerol as fermentation substrates, resulting in low production cost and high efficiency.

The present application relates to a kind of based on aconitate UV curing aluminum-based composite film and its preparation method and application, it solves the technical problems existing in interface bonding force, aging resistance and solidification efficiency of existing aluminum-plastic film, based on aconitate UV curing aluminum-based composite film includes aluminum base material and UV curing film layer;Aluminum base material surface is treated by vinyl silane coupling agent to form the covalent layer containing Si-O-Al covalent bond and carbon-carbon double bond;The UV curing film layer is by methacrylate, aconitate, crosslinking agent, photo-initiator is copolymerized by ultraviolet light curing;Wherein, aconitate is one or the mixture of more than two of various carbon number alkyl aconitate, cycloalkyl aconitate;The present application can be widely used in soft package battery, aluminum-plastic film, lithium battery packaging field.