32 results about "Aconitic acid" patented technology

The application provides a recombinant Aspergillus niger with high trans-aconitic acid yield and a construction method and application thereof, and relates to the technical field of genetic engineering. Specifically, the recombinant Aspergillus niger heterogeneously expresses aconitate isomerase TbrA and / or Adi1. The application provides a new engineering strain for efficient production of trans-aconitic acid, solves the source predicament of needing plant extraction or needing chemical synthesis in traditional production of trans-aconitic acid, and greatly improves the yield of trans-aconitic acid.

The invention provides a composite surfactant for oil displacement of a hypersalinity oil reservoir and a preparation method of the composite surfactant, and relates to the technical field of petrochemical engineering. The composite surfactant is prepared from the following components in percentage by weight: 0.1 to 0.3 percent of anionic-nonionic surfactant, 0.01 to 0.03 percent of cationic surfactant and the balance of formation water, wherein the anionic-nonionic surfactant is prepared by the following steps: reacting a hydroxyl-containing compound with ethylene oxide to obtain polyoxyethylene ether, then carrying out esterification modification by using aconitic acid modified rosin, then sulfonating by using a sulfonating agent, and finally neutralizing. The composite surfactant shows relatively low interfacial tension under the condition of hypersalinity (50000-200000 mg / L), is high in stability, and can be used for improving the recovery efficiency of hypersalinity oil reservoirs.

The invention discloses application of a biomarker in diagnosis of lung cancer soft meningeal metastasis. According to the present invention, it is found for the first time that the metabolites such as Indolastic acid, L-Glutic acid, N-Lactoylphenylalanine, cis-Acetic acid, Formiglumic acid and N-Acetylneomic acid in the cerebrospinal fluid have good diagnosis efficiency on the lung cancer soft meningeal metastasis, have high accuracy, high sensitivity and high specificity, can be used as the biological marker of the lung cancer soft meningeal metastasis, and can be used in the effective diagnosis of the lung cancer soft meningeal metastasis. The invention provides a brand-new thought and strategy for research and development of related diagnostic products for the lung cancer soft meningeal metastasis, and has wide application prospects and important transformation significance in the technical field of diagnosis of the lung cancer soft meningeal metastasis.

The invention relates to the technical field of concrete admixtures, and particularly discloses a rapid dispersing type polycarboxylate superplasticizer and a preparation method thereof.The rapid dispersing type polycarboxylate superplasticizer is prepared from, by mass, 200-300 parts of polyether monomer, 1.5-3.5 parts of oxidizing agent, 0.2-0.5 part of reducing agent, 1.2-3 parts of chain transfer agent, 25-33 parts of unsaturated acid, 10-20 parts of self-made functional monomer modified alkyl glycoside, 5-10 parts of aconitic acid and 250-300 parts of tap water. The preparation method comprises the following steps: dissolving, preparing a solution A, preparing a solution B and synthesizing to prepare the quick dispersing type polycarboxylic acid water reducing agent. By introducing alkyl glycoside, wetting of cement particles is promoted, water-cement interfacial tension is rapidly reduced, meanwhile, cement particle aggregation is hindered through stereoisomerization of alkyl glycoside, the effect of rapid dispersion of cement particles is achieved, and modified alkyl glycoside provides adsorption points and electrostatic repulsion of cement particles to hinder cement particle aggregation, so that the cement particles are dispersed rapidly. The cement particles can be quickly dispersed in a low-temperature environment.

The application discloses a terpolymer scale inhibitor and a preparation method thereof, and belongs to the technical field of polymer macromolecules and water treatment. The terpolymer scale inhibitor has the following general structure formula: wherein x, y and n are any integer in 1-10. In addition, the application further provides a preparation method of the terpolymer scale inhibitor, which comprises the following steps: mixing aconitic acid and water, stirring and dissolving at 50-55 DEG C, then adding N,N-dimethyl acrylamide and p-aminobenzoic acid, then increasing the temperature to 65-85 DEG C and adding an initiator dropwise, and then reacting at 65-90 DEG C to obtain the terpolymer scale inhibitor. The scale inhibitor is green and environment-friendly, and has excellent scale inhibition performance.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for evaluating the prognosis of patients with hypertrophic cardiomyopathy and application thereof. The application determines that one or more of 3-hydroxyrilotene, asparagine-lysine, cyclohexylamine, arachidonic acid, aconitic acid, propionyl carnitine, ceramide, tryptophan, 3-hydroxysebacic acid, traumatic acid, phosphatidylcholine, alanine-proline, lysophosphatidylcholine, taurine and S-garlicin cysteine are related to the prognosis of hypertrophic cardiomyopathy, can evaluate the prognosis of patients with hypertrophic cardiomyopathy after undergoing muscle resection of interventricular septum, and shows accurate judgment on all-cause death or rehospitalization, cardiovascular events and postoperative new-onset arrhythmia. Finally, all the prognostic biomarkers are reproduced in an independent cohort, which is helpful for postoperative risk evaluation of patients with hypertrophic cardiomyopathy, has high specificity and high sensitivity.

The invention relates to the technical field of gene engineering, and discloses a recombinant strain, a construction method and application thereof, and a method for producing total fatty acid. The recombinant strain is obtained by genetic modification of an original strain, and compared with the original strain, the recombinant strain overexpresses citric acid transporter Yhm2p, cis-aconitase E.HA and isocitrate dehydrogenase IDP located in cytoplasm at the same time, and does not express isocitrate dehydrogenase IDH1 and isocitrate dehydrogenase IDH2 located in mitochondria. The recombinant strain provided by the invention forms a high-efficiency pathway of citric acid retention-cytoplasm transport-directional production of NADPH, so that the core bottleneck of insufficient supply of cytoplasm NADPH in fatty acid synthesis is solved, the total fatty acid yield is remarkably increased, the transformation logic is accurate, and a stable and reliable source is provided for industrial high-efficiency production of fatty acid.

The invention provides an application of metabolites in sow vaginal secretion in early pregnancy diagnosis, and finds that three metabolic molecules can be used as markers for the early pregnancy diagnosis of sows for the first time on the basis of non-targeted metabonomics and machine learning conjoint analysis. According to the present invention, the metabolic markers are respectively INDOLEPROPIONIC ACID (indolepropionic acid), cis-Amonitate (cis-Amonitate) and 4-p-Coumaroylquinic acid (4-p-coumaroylquinic acid), the ROC curve AUC values of the metabolic markers are respectively 0.88, 0.92 and 0.77, and the metabolic markers have excellent diagnosis performance during the combination diagnosis. The pregnancy diagnosis is carried out by adopting the vaginal secretions of the sows 18 days after hybridization, the effective pregnancy diagnosis time is advanced by 5-8 days on the basis of a wave diagnosis method, and the method has important significance for reducing cost and increasing ultrasonic efficiency in live pig feeding.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The invention discloses a high-toughness composite PVC film and a preparation method thereof. The high-toughness composite PVC film is prepared from the following components in parts by weight: 62 to 74 parts of PVC resin, 54 to 67 parts of oxidized polyethlene wax, 25 to 35 parts of silicon dioxide, 26 to 39 parts of aconitic acid monoester, 14 to 28 parts of terephthalic acid, 24 to 36 parts of silane coupling agent, 16 to 28 parts of ethylene propylene diene monomer, 14 to 18 parts of glyceryl stearate, 14 to 19 parts of rare earth stearic acid and 16 to 26 parts of oleamide. Compared with a film at the present stage, the PVC film provided by the invention forms a more stable spatial network structure, can further increase the air permeability, can effectively play a supporting effect, has higher toughness, and is mainly embodied in high elongation at break, high falling standard impact strength, improved impact strength and improved air permeability of the film.

The invention belongs to the field of antioxidants, and particularly relates to a chitosan-based antioxidant as well as a preparation method and application thereof. The chitosan-based antioxidant provided by the invention is prepared by carrying out Michael addition reaction on an antioxidant with a structure as shown in a formula (I) and a biomass raw material and then carrying out amidation reaction with chitosan, the biomass raw material is one or more of itaconic acid, fumaric acid, mesaconic acid, muconic acid, aconitic acid, methacrylic acid, linoleic acid and terpenoids. According to the invention, a specific biomass raw material is taken as a bridging agent, and a small molecular antioxidant is grafted into chitosan through Michael addition reaction and amidation reaction, so that the molecular weight of the antioxidant is increased, and the thermal stability and migration resistance of the antioxidant are improved; meanwhile, a chitosan structure exists in the antioxidant, residual metal ions in the rubber emulsion can be chelated, the catalytic oxidation effect of the metal ions is inhibited, and the oxidation resistance of the antioxidant is improved.

The invention provides a high-density drilling fluid with high temperature resistance and a preparation method thereof, and the high-density drilling fluid comprises the following components in parts by weight: 100 parts of water; 0.5 to 4 parts of a clay substance; 1-6 parts of a non-differentiated functional long-acting treatment agent; 1-6 parts of a humic acid auxiliary material; 1-4 parts of a pH value regulator; 0 to 15 parts of salt; 200 to 250 parts of a weighting agent; the non-differentiated function long-acting treating agent is prepared by carrying out polymerization reaction on an esterified aconitic acid monomer, an itaconic acid monomer, an acrylic acid monomer and a 2-acrylamido-2-methylpropanesulfonic acid monomer according to a certain mass ratio. The high-density drilling fluid is simple in composition and preparation, the rheological property and high-temperature and high-pressure filter loss of the drilling fluid are easy to control, the long-term high-temperature stability is greatly improved, the high-density drilling fluid can be applied to drilling construction of high-temperature and high-pressure strata, the components and dosage of a treating agent are greatly reduced, and the long-term high-temperature stability of the high-temperature and high-density drilling fluid is improved; and well drilling construction is facilitated.

Provided is a novel cis-aconitate synthesis enzyme, more particularly, a recombinant microorganism for producing itaconate including a cis-aconitate synthesis enzyme variant. According to the present disclosure, it was confirmed that the production and yield of itaconate were significantly increased in the recombinant microorganism for producing itaconate into which the novel cis-aconitate synthesis enzyme was introduced. In addition, it was confirmed that in the recombinant microorganism for producing itaconate of the present disclosure, a new carbon flow to itaconate was separated from the existing TCA cycle based on the activity of the corresponding enzyme. Accordingly, the novel aconitate synthesis enzyme of the present disclosure and the recombinant microorganism introduced with the aconitate synthesis enzyme can increase the economic feasibility of itaconate, and thus can be used in various industrial fields, such as synthetic resins, latexes, and food additives in which itaconate is used.

The application provides a recombinant Aspergillus niger strain with high trans-aconitic acid yield, and belongs to the technical field of genetic engineering. The recombinant Aspergillus niger strain can simultaneously express aconitase and aconitate isomerase. The genetically engineered Aspergillus niger strain provided by the application has a generally higher trans-aconitic acid yield than a starting strain, and the strain with the highest yield can reach 51 g / L at a shake flask level, and has a very strong application value.

The application belongs to the technical field of rice cultivation and relates to a plant factory cultivation method for shortening the growth period of rice based on carbon and nitrogen nutrition regulation. In view of the technical problem that the existing plant factory rice cultivation method has a long growth period and is difficult to meet the rapid cultivation demand, the method comprises seed soaking, germination, seedling raising, post-transplanting management and harvesting, and the core is to transplant rice seedlings to a plant factory water culture system, and synchronous control of cultivation conditions is used to promote early flowering: maintaining CO2 concentration of 400-1600 ppm, using full-spectrum artificial light source, using basic nutrient solution with total nitrogen of 1.2-3.2 mM, molar ratio of ammonium nitrogen to nitrate nitrogen of 2:1-4:1 and pH of 5.5-6.5, and adding L-carnitine and aconitic acid metabolism promoters with a molar ratio of 1.2:1-2.2:1 at the tillering peak stage. The method is mainly used for rapid cultivation of rice in a plant factory, and the cultivation efficiency is improved by shortening the growth period.

The invention discloses a construction method of escherichia coli for efficiently synthesizing glycollic acid, and belongs to the technical field of biochemical engineering. According to the invention, citric acid synthase gltA and aconitic acid synthase acnA and acnB are overexpressed in escherichia coli cells, pyruvate dehydrogenase transcription inhibition factor pdhR is knocked out, transhydrogenase pntA and pntB are overexpressed, and transhydrogenase stA is knocked out, so that the constructed recombinant escherichia coli can produce glycollic acid by using glucose in a short time. The reaction cost is lower, the yield is higher, the concentration of glycollic acid produced after fermentation in a 5L fermentation tank for 48 hours is 81 g / L, the conversion rate is 0.62 g / g, and the production intensity is 1.69 g / L / h.

The invention discloses a bio-based electrolyte additive, a preparation method and application, the bio-based electrolyte additive is N-(3-indoleethyl)-5-oxopyrrolidine-2, 3-dicarboxylic acid, the raw material of the additive is derived from renewable biomass resources, a tryptophan precursor can be derived from microbial metabolism of natural tryptophan, and the tryptophan precursor can be derived from natural tryptophan. The trans-aconitic acid can be directly extracted from plants such as aconite, and the N-(3-indoleethyl)-5-oxopyrrolidine-2, 3-dicarboxylic acid is synthesized through two green and renewable bio-based raw materials. As an electrolyte additive of a water-based zinc battery, the tryptamine-trans-aconitic acid composite electrolyte fuses interface adsorption of tryptamine and ion coordination capability of trans-aconitic acid, synergistically constructs a stable electrode-electrolyte interface, can synergistically inhibit side reactions such as growth of zinc dendrites and hydrogen evolution, significantly alleviates zinc negative electrode corrosion, realizes a long-cycle-life water-based zinc battery, and has a wide application prospect. The cycle life of the aqueous zinc battery reaches 2500 hours.

The invention discloses a recombinant yarrowia lipolytica strain with high yield of itaconic acid and a construction method and application thereof, and belongs to the field of strain cultivation, the recombinant yarrowia lipolytica strain is obtained by metabolism reconstruction of a wild type yarrowia lipolytica strain, a cis-aconitic acid decarboxylase gene cadA derived from Aspergillus terreus ATCC20542 is inserted into a genome, and a stable expression cassette is formed through a TEF1 promoter and a CYC1 terminator; a CIT1 gene front-end signal peptide is fused at the N end of the cadA to realize directional expression of mitochondria; iDH1, ACL1 and ADH1 genes are knocked out at the same time; cIT1 and ACO1 genes are overexpressed to enhance the flux of citric acid in the direction of cis-aconitic acid; and the noxE gene of Lactococcus lactis is introduced to regulate the balance of NADH (Nicotinamide Adenine Dinucleotide Hormone) in the cell. The strain is fermented for 72 hours under the conditions that glucose is used as a carbon source, the temperature is 30 DEG C and the pH value is 5.5-6.0, and the yield of itaconic acid can reach 12 g / L or above. According to the construction method, gene knockout and homologous recombination integration are realized by adopting CRISPR-Cas9, and a target strain is obtained after screening and verification. The method is used for preparing degradable polymers, plasticizers and medical intermediates. The method has the beneficial effects that the itaconic acid yield and metabolism efficiency are improved.

The present application discloses a highly water-soluble paclitaxel compound chemically modified with a bisaconitic acid molecule, a synthesis method, and applications. The compound comprises paclitaxel and two aconitic acid molecules covalently bonded via an ester bond. The preparation method of the water-soluble paclitaxel compound is simple and suitable for industrial production. The paclitaxel drug molecule, after structural modification by the method of the present application, possesses amphiphilic properties, i.e., both hydrophilic and lipophilic properties. The improved water solubility and permeability provide the possibility of developing various oral and aerosol inhalation drug formulations. The compound has high water solubility at room temperature, dissolves without foaming, and can reach a concentration of more than 8 mg / ml without requiring any cosolvent. The compound has lower toxicity than the original drug paclitaxel. The ester bond of the compound undergoes accelerated hydrolysis and cleavage under the weakly acidic conditions of tumor tissue in vivo, thereby exhibiting certain tumor tissue targeting properties, good bioavailability, and anti-tumor effects.

The application provides a gastric juice metabolite composition and application for early gastric cancer screening, a method for screening early gastric cancer related metabolites and an early gastric cancer disease risk prediction method, and provides a gastric juice metabolite composition for gastric cancer screening, which is composed of glutamic acid, cis-aconitic acid, methionine and taurine.The application can realize non-invasive sampling independent of gastroscopy based on the sampling capsule mode, and finally provides an effective non-invasive screening method for early gastric cancer for the clinic.Through non-invasive gastric juice acquisition and marker detection, non-invasive auxiliary diagnosis of gastric cancer can be realized, the compliance of the subject is improved, and the body damage is reduced.

The application discloses an in-situ hydrophobic supramolecular system for separating and purifying aconitic acid and a method for separating and purifying aconitic acid, relates to the technical field of aconitic acid separation and purification, and the in-situ hydrophobic supramolecular system comprises a hydrogen bond acceptor neutral amine which is hardly soluble in water and a diluent, and the diluent is one or more of n-hexanol, n-octanol, 2-octanol, isopentyl ether, n-hexyl ether and the like. Therefore, the application provides the in-situ hydrophobic supramolecular system for separating and purifying aconitic acid and the method for separating and purifying aconitic acid, the in-situ hydrophobic supramolecular system has the advantages of good stability, high recovery rate and easy recycling, and the like, the aconitic acid product prepared by the method has high yield and high purity, the method is simple, effective, green, energy-saving, environment-friendly and the like.

The invention discloses application of biological energy active molecules in preparation of medical instruments for skin, and belongs to the technical field of medical instruments. In the application, the biological energy active molecules comprise at least one of succinic acid, alpha-ketoglutaric acid, malic acid, citric acid, cis-aconitic acid and fumaric acid. Biological energy active molecules are applied to preparation of hydrogel medical instruments for skin wound healing and / or scar inhibition, and are combined with natural polymer biological materials, so that on one hand, the obtained hydrogel has excellent biocompatibility, mechanical properties, air permeability, water absorption and storage stability; on the other hand, the hydrogel dressing also has multiple effects of supplying energy, inhibiting oxidative stress, regulating and controlling an inflammation microenvironment and the like, rapid wound healing is realized, wound scar formation is reduced, and the problems that an existing hydrogel dressing for the skin is relatively single in performance and function and difficult to meet the rapid wound healing requirement in a complex pathological environment are solved; wide application prospects are realized.

The application provides a CO2 capture method and a CO2 capture device coupled with electrochemical regeneration of hydrogen. The method comprises the following steps: absorbing CO2 in a CO2-containing gas by using an alkaline solution to form a CO2-rich solution; performing acid-base neutralization of the CO2-rich solution and an acidic solution to obtain a neutralization solution and CO2; performing electrolysis of the neutralization solution to obtain a cathode electrolysis product and an anode electrolysis product, wherein the cathode electrolysis product is H2 and the alkaline solution, and the anode electrolysis product is O2 and the acidic solution; and the acidic solution is selected from one or a combination of more than two of the following solutions: pyruvic acid solution, gluconic acid solution, malonic acid solution, tartaric acid solution, glyceric acid solution, oxalacetic acid solution, formic acid solution, isopropionic acid solution, isobutyric acid solution, fumaric acid solution, aconitic acid solution, isocitric acid solution and hexanediol diacid solution. The acidic solution generated by electrolysis can avoid corrosion of the device equipment and reduce the net energy consumption and net cost of the CO2 capture process.

To provide a tree trunk injection agent having good stability of an active ingredient.SOLUTION: A tree injection containing an aqueous alcohol solution soluble compound such as acetic acid, hydroxyacetic acid, methacrylic acid, acrylic acid, oxalic acid, malonic acid, succinic acid, adipic acid, tartaric acid, diethyl tartrate, trans-aconitic acid, citric acid, gallic acid, phthalic acid, isophthalic acid, terephthalic acid, butanediol, trimethylolpropane, glycerol, dithiothreitol, dithioerythritol, sorbitol, maltitol, and reduced starch syrup, a monohydric alcohol having 1 to 4 carbon atoms such as methanol, ethanol, 1-propanol, 2-propanol, and 1-butanol, acetamiprid, and water.SELECTED DRAWING: None

The invention relates to the technical field of pharmaceutical co-crystals, and discloses a progesterone co-crystal as well as a preparation method and application thereof, and the progesterone co-crystal takes progesterone as a pharmaceutical active component and trans-aconitic acid as a co-crystal formation. The progesterone is used as an active pharmaceutical ingredient, the trans-aconitic acid is used as a co-crystal former, carbonyl in the progesterone serving as the active pharmaceutical ingredient is a good receptor for forming a hydrogen bond, carboxyl in the trans-aconitic acid is a hydrogen bond donor, and a strong intermolecular hydrogen bond is formed between the progesterone and the trans-aconitic acid, so that the progesterone and the trans-aconitic acid are co-crystallized. The progesterone eutectic is successfully synthesized under the intermolecular acting force of hydrogen bonds, and compared with single progesterone, the progesterone eutectic is improved in solubility, permeability and bioavailability.

The invention relates to a bio-based composite plasticizer and a preparation method and application thereof, and solves the technical problems that the existing bio-based plasticizer is high in energy consumption, easy to cause environmental pollution and unsatisfactory in plasticizing effect, the bio-based composite plasticizer contains citrate, and also contains 0.68-23.85% by mass of aconitic acid ester and 0.1-0.5% by mass of fumarate, the mass percent of the fumarate is 1.38%-8.8%, and the balance is the citrate. The method can be widely applied to the field of preparation of high polymer materials.

This invention belongs to the field of water treatment technology, specifically disclosing a green scale inhibitor for barium sulfate and its preparation method. The scale inhibitor is a copolymer formed by the esterification reaction of polysuccinimide and hydroxyethyl methacrylate under alkaline conditions, aconitic acid, and sodium lignosulfonate graft copolymerization, wherein the mass ratio of polysuccinimide / hydroxyethyl methacrylate / aconitic acid / sodium lignosulfonate is 2:4:(1-6):(1-4). The scale inhibitor of this invention is a composite copolymer formed by the polymerization and grafting of the esterified product of polysuccinimide / hydroxyethyl methacrylate, aconitic acid, and sodium lignosulfonate. It simultaneously possesses carboxyl, hydroxyl, amino, and sulfonic acid groups, which can strongly interact with key ions or sites in the BaSO4 crystal growth process, preventing the normal growth and aggregation of BaSO4 crystals, thereby effectively inhibiting the formation of BaSO4 scale.

This invention provides a method for preparing trans-aconitic acid using Aspergillus terrestris fermentation and its application, relating to the field of fermentation engineering technology. By adjusting the pH and dissolved oxygen (DO) during the fermentation process, this invention can significantly increase the trans-aconitic acid content in the fermentation broth. Simultaneously, it solves the problem of excessively high production costs caused by low trans-aconitic acid content during subsequent separation and purification, greatly reducing the production cost of trans-aconitic acid, simplifying the production steps, and providing a simple and widely applicable method for trans-aconitic acid production.

This invention provides a recombinant *Yarrowia lipolytica* strain, its construction method, and a method for preparing trans-aconitic acid. The provided recombinant *Yarrowia lipolytica* carries the following nucleotide fragments: a nucleotide fragment encoding aconitic acid isomerase and a nucleotide fragment encoding a mitochondrial tricarboxylic acid transporter. *Yarrowia lipolytica* is a biosafe strain, and the recombinant *Yarrowia lipolytica* strain constructed using it can synthesize trans-aconitic acid using common carbon sources such as glucose and glycerol as fermentation substrates, resulting in low production cost and high efficiency.

The invention discloses a Zymomonas mobilis recombinant strain for producing itaconic acid and application of the Zymomonas mobilis recombinant strain. Zymomonas mobilis ZMNP-T7 is taken as a starting strain of the recombinant strain, and cis-aconitic acid decarboxylase gene cadA is integrated at the 1650 site of a genome; a cis-aconitase gene CgacnA is overexpressed, an endogenous isocitrate dehydrogenase gene citC of the recombinant strain is subjected to promoter mutation, and an initiation codon ATG is replaced by GTG or TTG; wherein the nucleotide sequences of the gene cadA, the gene CgacnA and the gene citC are shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3 in sequence. Zymomonas mobilis (ZMNP-T7) is taken as a starting strain, production of itaconic acid in the Zymomonas mobilis is realized by introducing a specific exogenous gene cadA, and then the yield of itaconic acid is improved by increasing the copy number of itaconic acid synthesis genes, screening cis-aconitase (Aco) and reducing the activity of isocitrate dehydrogenase.