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Seed selection method of cabbage type brassica juncea cytoplasmic male sterile line

A technology of Brassica napus and male sterile lines, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problem of single cytoplasm, and achieve the effects of stable inheritance, high efficiency, and simple method

Inactive Publication Date: 2012-05-30
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the cytoplasm of the existing rapeseed cytoplasmic male sterile type is single, and the male sterile line produces a trace amount of normal fertile pollen under low temperature or high temperature conditions, and proposes A Breeding Method for Cytoplasmic Male Sterile Line and Maintainer Line of Brassica napus

Method used

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  • Seed selection method of cabbage type brassica juncea cytoplasmic male sterile line
  • Seed selection method of cabbage type brassica juncea cytoplasmic male sterile line
  • Seed selection method of cabbage type brassica juncea cytoplasmic male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Breeding a cytoplasmic male sterile line of Brassica napus with Huashuang 4 genetic background

[0022] (1) The cytoplasmic male sterile line WJS1A (2n=36, AABB) of mustard type rapeseed, which is derived from the natural sterile plant of the mustard type rapeseed farm variety Yuxi Gaoke in Yunnan Province, WJS1A was delivered on November 26, 2010 The Chinese Type Culture Collection Center in Wuhan University, Wuhan City, Hubei Province, and its preservation number is CCTCC NO: P201018) is the female parent, and Beijing Chinese cabbage (B.rapa, 2n=20, AA) is the male parent for distant hybridization , to harvest F 1 generation hybrid seeds;

[0023] (2) The F obtained in step (1) 1 Generation hybrid seeds are planted in the field and harvested F 1 free pollinated F 2 substitute seeds;

[0024] (3) The F obtained in step (2) 2 Seeds are planted in the field. in F 2 The selected male sterile plants were testcrossed with the Brassica napus variety Huashu...

Embodiment 2

[0030] Example 2: Breeding a cytoplasmic male sterile line of Brassica napus with the genetic background of Zhongshuang 7

[0031] (1) The cytoplasmic male sterile line WJS1A (2n=36, AABB) of mustard type rapeseed, which is derived from the natural sterile plant of the mustard type rapeseed farm variety Yuxi Gaoke in Yunnan Province, WJS1A was delivered on November 26, 2010 The Chinese Type Culture Collection Center in Wuhan University, Wuhan City, Hubei Province preserves, its preservation number is CCTCC NO: P201018) as the female parent, Chinese cabbage (B. species) as the male parent to carry out distant hybridization, and harvest the hybrid F 1 seeds;

[0032] (2) The obtained hybrid F 1 Seeds are planted in the field. Harvest F 1 F 2 substitute seeds;

[0033] (3) The F obtained in step (2) 2 Seeds are planted in the field. in F 2 In the generation, the male sterile plants were selected as the male parent for test crossing with the double low cabbage rape variet...

Embodiment 3

[0039] Example 3: Comparison of the Restoration Relationship between the Cytoplasmic Male Sterile Line of Brassica napus and Other Rapeseed CMS Lines

[0040] Eight Brassica napus cultivars or lines (Hui 01, 91 Hui-5900, 25CC1, Huashuang 3, Huashuang 4, Huayou 8, Zhongyou 821 and Zhongshuang 7) were selected and compared with WNJ01A CMS, Pol CMS , Ogu CMS and Kos CMS for testcross, the harvested F 1 The hybrids were planted in the field, and the fertility identification was carried out at the flowering stage. The results of the recovery relationship determination are shown in Table 1. The recovery relationship of WNJ01A CMS is different from that of Pol CMS, Ogu CMS and Kos CMS, indicating that WNJ01A CMS is a cytoplasmic type different from Pol CMS, Ogu CMS and Kos CMS.

[0041] Table 1 The results of determination of the relationship between four kinds of cytoplasmic sterile lines

[0042]

[0043] Note: (1) S in Table 1 means sterile, and F means fertile;

[0044] (2)...

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Abstract

The invention relates to a seed selection method of a cabbage type brassica juncea cytoplasmic male sterile line. The invention belongs to the technical field of crop seed breeding. The method is characterized in that: a brassica juncea cytoplasmic sterile line WJS1A (AABB) is hybridized with a Chinese cabbage variety of Shanghai cabbage or Beijing cabbage with a genome of AA; in an F2 generation, sterile plants with cabbage type rape characteristics are selected as female parents, and are subject to male parent hybridization with elite cabbage type rape varieties such as Huashuang No.4 (AACC), such that male parent selfed seeds and hybridization seeds are obtained; the seeds are seeded, and F1 plant fertility is assessed during a flowering phase; sterile plants shown infertility combinations are backcrossed for 3-4 generations by using the original male parent, until a cabbage type brassica juncea cytoplasmic male sterile line WNJ01A with stable male sterile characteristics is obtained. The recurrent parent Huashuang No.4 is a maintainance line of the sterile line. As a result of the restoring and maintaining of the relationship, the WNJ01A sterile line is a novel sterile cytoplasm type different from polima cytoplasmic male sterility (Pol CMS), radish cytoplasmic male sterility (Ogu CMS), and Kos cytoplasmic male sterility (Kos CMS). With the method provided by the invention, the obtained WNJ01A sterile line is a novel cytoplasmic male sterile type of rapes and cruciferae crops, and assists in establishing a firm germplasm basis for heterosis utilization of the crops.

Description

technical field [0001] The invention relates to a breeding method for a cytoplasmic male sterile line of Brassica napus and mustard, belongs to the technical field of crop breeding, and is specially used for utilization of heterosis in Brassica napus. Background technique [0002] Using cytoplasmic male sterile line (CMS), maintainer line and restorer line to produce hybrids is the main way to utilize rapeseed heterosis. The types of CMS in rape that have been reported at home and abroad include Pol CMS, Shan 2A CMS, Ogu CMS, Kos CMS, Nap CMS and Tour CMS. According to its source, it can be divided into two categories: the first type of homologous cytoplasmic male sterility, that is, male sterility caused by natural mutation during plant reproduction and hybridization between varieties, including Pol CMS, Shaanxi 2A CMS and Nap CMS. Among them, the Pol CMS system has become the most widely used rapeseed cytoplasmic male sterile material in my country because it was the firs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 吴江生石淑稳刘克德刘超王小峰
Owner HUAZHONG AGRI UNIV
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