Molecular marker method of turnip mosaic virus resistance gene in non-heading Chinese cabbage

A technology of turnip mosaic virus and head cabbage, applied in biochemical equipment and methods, material analysis through observation of the influence on chemical indicators, microbial measurement/inspection, etc., can solve the problem of non-heading cabbage resistance to turnip The gene of cyanine mosaic virus has not been reported, etc.

Inactive Publication Date: 2009-03-25
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Although domestic and foreign studies on TUV-resistant genes have been carried out for ten years, researches on non-heading Chinese cabbage TUV-resistant genes have not been reported.

Method used

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  • Molecular marker method of turnip mosaic virus resistance gene in non-heading Chinese cabbage
  • Molecular marker method of turnip mosaic virus resistance gene in non-heading Chinese cabbage

Examples

Experimental program
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Effect test

Embodiment 1

[0029]Material screening: Using non-heading Chinese cabbage from the Horticultural Research Institute of Shanghai Academy of Agricultural Sciences as the test material, 5 parts of disease-resistant materials and 3 parts of susceptible materials, using AFLP (Amplified fragment length polymorphism, amplified fragment length polymorphism) Marking, using the DPS data processing system, constructing a clustering dendrogram according to UPGMA, and selecting the disease-resistant material Q048 with the lowest genetic similarity, that is, the most distant genetic relationship, and the disease-resistant material A168-5D as materials in this example. The materials and samples used can be obtained from the Institute of Horticulture, Shanghai Academy of Agricultural Sciences through public channels such as purchase.

[0030] Cultivation of materials: After selecting full seeds to germinate, sow them in a matrix mixed with peat, vermiculite and organic fertilizer (volume ratio 4:4:1), pour ...

Embodiment 2

[0072] Material screening: non-heading Chinese cabbage from the Institute of Horticulture, Shanghai Academy of Agricultural Sciences was used as the test material, 5 parts of disease-resistant materials, 3 parts of disease-resistant materials, using AFLP (Amplified fragment length polymorphism, amplified fragment length polymorphism) for labeling , using the DPS data processing system, constructing a clustering dendrogram according to UPGMA, and selecting the disease-resistant material Q048 with the lowest genetic similarity, that is, the most distant genetic relationship, and the susceptible material A168-5D as materials in this example.

[0073] Cultivation of materials: After selecting full seeds to germinate, sow them in a matrix mixed with peat, vermiculite and organic fertilizer (volume ratio 4:4:1), pour water and drill 0.5 cm deep holes, put the seeds in, Then cover with 0.5 cm thick peat. Put the plug trays into a greenhouse with an insect-proof net for cultivation, t...

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Abstract

The invention relates to a molecular marker method of a non-heading Chinese cabbage turnip-mosaic-virus-resistance gene, including screening and cultivating of turnip-mosaic-virus-resistance non-heading Chinese cabbage parent materials, parent hybridization and F1 selfing, vaccination and testing of turnip mosaic viruses, DNA extraction of non-heading Chinese cabbage materials and the establishment of a resistance pool, the molecular marker screening of resistance genes, calculation of exchange rate and distance of resistance gene linked markers and establishment of a resistance gene linked marker map and the like; wherein, the molecular marker technology is applied to screen the parents of the turnip-mosaic-virus-resistance non-heading Chinese cabbage and the marking of the linked genes of the resistance genes; and different molecular markers have different reaction systems and amplification procedures. The method constructs the molecular marker procedures and systems of turnip-mosaic-virus-resistance genes with non-heading Chinese cabbage as material and provides bases for positioning the non-heading Chinese cabbage turnip-mosaic-virus-resistance gene on chromosome, resistance gene cloning and assisted breeding research of resistance molecules.

Description

technical field [0001] The invention relates to a method for screening virus resistance gene molecular markers in the field of agricultural technology, in particular to a molecular marker method for non-heading cabbage resistance to turnip mosaic virus genes. Background technique [0002] Non-heading cabbage (also known as green cabbage) is a very important popular green leafy vegetable in my country, and it is supplied to the market all year round. However, viral diseases, especially turnip mosaic virus, are seriously harmful to non-heading cabbage, and turnip mosaic virus is transmitted through aphids , spreads rapidly, infects plants systematically, and has an incubation period. After the onset, mosaic leaves and diseased spots will be produced, resulting in a decline in vegetable quality and crop yield. There is no pesticide to cure it, and once it occurs, it will suffer heavy losses. The commonly used control method is to control the occurrence and spread of aphids. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53G01N21/78
Inventor 陈火英王新华刘杨庄天明
Owner SHANGHAI JIAO TONG UNIV
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