The invention discloses a liquid fertilizer prepared from microorganisms and suitable for green vegetables and a preparation method of liquid fertilizer. The liquid fertilizer comprises 1-5 parts of a microbial zymophyte liquid, 10-35 parts of an organic fertilizer, 2-5 parts of nutrient elements, 2-5 parts of chlorophyll, 5-10 parts of potassium salt and 10-20 parts of water. The microbial zymophyte liquid is obtained by respectively fermenting rhizoma bletillae endophytic trichoderma and eucommia endophyte through a culture medium. The preparation method comprises the following steps: adding microorganisms into a carbon source and a nitrogen source according to a certain ratio, conducting fermenting, adding the obtained microbial zymophyte liquid into the organic fertilizer, carrying out secondary fermentation, carrying out solid-liquid separation, adding the nutrient elements, chlorophyll and monopotassium phosphate into secondary fermentation liquid according to prescription amounts, and conducting stirring to dissolve the nutrient elements, the chlorophyll and the monopotassium phosphate, thereby obtaining the liquid fertilizer. The problem that pesticide residues of green vegetables easily exceed the standard and food safety and environment problems caused by long-term excessive use of chemical fertilizer can be solved; and it is guaranteed that the fertilizer can provide sufficient and various nutrients, and meanwhile, natural effective components with a pest control function are added, so operation of farmers is facilitated, the labor cost is reduced, the use of chemical fertilizers and pesticides is reduced, the purposes of improving the edible safety of the green vegetables and effectively utilizing eutrophic fermentation liquor are achieved, and the effect of environmental protection is achieved.