The invention relates to a disease-resistant wood vinegar water-soluble fertilizer as well as a preparation method and application thereof. The disease-resistant wood vinegar water-soluble fertilizercontains the following raw materials in parts by weight: 50-80 parts of wood vinegar, 30-40 parts of mashed garlic, 45-55 parts of crofton weed, 50-60 parts of peanut shell, 5-10 parts of potassium fulvate, 5-20 parts of potassium dihydrogen phosphate, 5-20 parts of ammonium dihydrogen phosphate, 1-10 parts of magnesium sulfate, 1-10 parts of ferrous sulfate, 1-5 parts of boric acid, 1-2 parts ofammonium molybdate, 1-3 parts of manganese sulfate, 1-3 parts of zinc sulfate, 0.5-1 part of copper sulfate, 5-15 parts of citric acid, 500-800 parts of water and 0.3 part of a surfactant. The water-soluble fertilizer is rich in nutrients, the nutrients do not mutually have antagonistic action, the balanced supplementation of various nutrients of crops can be realized, the water-soluble fertilizerdoes not precipitate, is capable of promoting the balanced absorption of plants to the nutrients and has excellent antibacterial and sterilizing effects, and the occurrence rates of plant diseases and insect pests are low; and the operation steps during the preparation are simple and reasonable in design, various components are easily available, the cost is low, and the maximum utilization of theresources is realized.