63 results about "Plant enzyme" patented technology

The invention relates to the technical field of health care product preparation, and particularly discloses an oyster peptide enzyme oral liquid capable of enhancing immunity and a preparation method thereof, the oyster peptide enzyme oral liquid comprises the following components: 20 parts of oyster peptide, 50 parts of plant enzyme liquid, 5 parts of honey, 2 parts of fructo-oligosaccharide, 2 parts of lemon juice and 100 parts of purified water; the oyster peptide enzyme oral liquid contains rich natural antioxidant ingredients including oyster peptide and plant enzyme liquid, the oyster peptide contains a large amount of sulfur-containing amino acid, and the ingredients can be used as antioxidants to reduce generation of free radicals and protect cells from oxidative damage; the plant enzyme is rich in natural antioxidant enzyme, can promote synthesis of antioxidant enzyme in vivo and remove free radicals in vivo so as to slow down senescence and diseases, the oyster peptide and the enzyme act together to improve the activity of catalase in vivo, and oxidative stress is reduced by removing superoxide anion free radicals.

The invention relates to whitening and stain-removing toothpaste and a preparation method thereof, and belongs to the technical field of oral care. The toothpaste contains 1.5-2.5 parts of plant zymolyte, 15-25 parts of composite abrasive and the like, and the plant zymolyte is obtained by grinding walnut shell powder, beech, oyster shell and Manny root ice river mud into powder according to the mass ratio of 3: 3: 2: 1 and mixing the powder with compound enzyme liquid for enzymolysis; the composite friction agent and the chelating agent are respectively prepared by mixing substances with specific particle sizes / molecular weights according to a ratio, and also contain a film-forming agent, an enamel repairing component and the like. The preparation method comprises the following steps: preparing plant zymolyte; preparing a mixed solution; and subsequently stirring, degassing and filling. Through cooperation of multiple components, the toothpaste achieves efficient whitening, stain removal and bacteriostasis, has the effects of long-acting stain prevention, enamel repair and oxidation resistance, and solves the problems that traditional toothpaste is not thorough in stain removal, damages enamel and the like.

The invention relates to a microbial agent for preventing and treating the root knot nematode disease of angelica sinensis and application of the microbial agent. The microbial agent is prepared from the following components: bacillus velezensis with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2022923, bacillus paraamycoides with the preservation number of CCTCC No: M2022924, a szechwan Chinaberry fruit extract, amino-oligosaccharin, fulvic acid and a medium trace element fertilizer. When the microbial agent is used in the angelica sinensis planting process, the root activity, the root enzyme activity, the plant enzyme activity and the proline content can be remarkably improved, and the nematode resistance of crops is improved; the pH, medium trace elements, enzyme activity and microbial diversity index in the soil are remarkably improved, the phenolic acid content of the soil is remarkably reduced, and the soil environment is improved; the Chinese angelica root-knot nematode disease prevention and treatment method has the advantages that the Chinese angelica root-knot nematode disease prevention and treatment effect is obvious, and the Chinese angelica root-knot nematode disease prevention and treatment method has a wide application prospect. The Chinese angelica root-knot nematode disease prevention and treatment method can be used for preventing and treating the Chinese angelica root-knot nematode disease and preventing and treating the Chinese angelica root-knot nematode disease.

The invention discloses a preparation method of a whole-bean rumex hanus nutrient plant enzyme, which comprises the following steps: S1, taking soybeans, and preparing whole-bean minced soybean; taking rumex hanus to prepare rumex hanus whole pulp; taking grains, and preparing whole grain pulp paste; taking fruits and vegetables, and preparing fruit and vegetable puree; s2, mixing the whole-bean minced soybean, the rumex hanus whole pulp, the whole-grain minced pulp and the fruit and vegetable primary pulp obtained in the step S1 to obtain whole-bean rumex hanus nutrient plant enzyme primary pulp, and then sequentially performing enzymolysis by combining cellulase with pectinase, amylase, saccharifying enzyme and protease; and S3, taking the whole-bean rumex hanus nutrient plant enzyme primary pulp subjected to enzymolysis in the step S2, performing anaerobic fermentation by using saccharomycetes and / or distiller's yeast under the negative pressure condition of-0.1 to-0.06 MPa, and filtering to obtain the whole-bean rumex hanus nutrient plant enzyme. The invention further provides the whole-bean rumex hanus nutrient plant enzyme. According to the technical scheme, the defects of traditional bean products, rumex hanus food and edible plant enzymes can be overcome.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a moisturizing effect and a preparation method and application thereof. The composition comprises the following components in percentage by weight: 0.3%-0.8% of a plant fermentation product, 1.2%-2.0% of a plant enzymolysis product, 15%-20% of a humectant, 1.6%-2.5% of a chondrus crispus extract, 0.5%-1.1% of a Chinese herbaceous peony extract, 2.6%-3.9% of a rosemary extract and the balance of water. The composition is suitable for people with allergic reaction caused by face dryness, has a good moisturizing effect, has a long-acting moisturizing effect, also has repairing and anti-aging effects, is mild and non-irritant, and can be used for preparing face moisturizing products.

The invention relates to the field of feed raw materials, and discloses a feed plant enzymolysis protein with a strong food calling effect and a preparation method thereof, the preparation method comprises the following steps: S1, dispersing and reducing viscosity: reducing the viscosity of a protein powder raw material through shearing force generated by high-speed rotation of a dispersing disc; step S2, enzymolysis of polysaccharide substances: plant protein powder is added into a reaction kettle in which water is added in advance, then polysaccharide enzymes are added, and enzymolysis is performed; step S3: proteolysis: adding sodium carbonate to adjust pH, adding compound protease into the reaction kettle, and performing enzymolysis; and S4, conveying the wet material to a spray drying tower for spray drying. According to the method, the pea powder syrup protein powder, the wheat protein powder and the soybean concentrated protein powder are used as raw materials, through dispersion, enzymolysis and spray drying, flavor substances are generated, the bitter taste is eliminated, the palatability of plant protein is remarkably improved, the strong food calling performance is shown in feeding experiments of piglets and cats, and the method is few in steps, mild in reaction condition and low in cost.

The invention relates to the technical field of health-care food, and discloses a health-care composition containing microbial beneficial bacteria and a preparation method of the health-care composition. Comprising the following components in parts by weight: 30-40 parts of gelatinized starch, 8-15 parts of plant enzyme powder, 2-10 parts of microcapsule compound beneficial bacteria, 3-5 parts of selenium-enriched yeast powder, 0.5-2 parts of an American ginseng extract, 12-20 parts of fructo-oligosaccharide, 1-2.5 parts of curcumin, 7-9 parts of ovalbumin and 5-8 parts of sodium carboxymethyl cellulose. Microcapsule composite beneficial bacteria are prepared by compounding bifidobacterium longum, lactobacillus acidophilus, lactobacillus rhamnosus and lactococcus lactis, bridging with carboxymethylated beta-glucan and a Fe < 3 + >-polyphenol network structure and embedding with a protein-polysaccharide system, and can stably exist in an environment with strong acid and high-concentration bile salt to gastrointestinal tracts; through the synergistic effect of different nutrient substances, the intestinal microbe balance can be improved, and the immunity can be remarkably improved.

The invention belongs to the technical field of cactus polysaccharide, and discloses a method for extracting cactus polysaccharide by compound enzyme, which comprises the following steps: S1, pretreating fresh cactus to obtain cactus powder, sealing and storing; s2, adding a compound enzyme into the cactus powder, and carrying out polysaccharide extraction by utilizing ultrasonic waves; wherein the compound enzyme is any two or three of papain, cellulase and pectinase; s3, after ultrasonic extraction, enzyme deactivation and centrifugation are conducted, an upper-layer crude extracting solution is taken and concentrated, ethanol is added for precipitation, standing and centrifugation are conducted, precipitates continue to be washed with absolute ethyl alcohol, acetone and diethyl ether respectively, drying is conducted, and crude cactus polysaccharide is obtained. According to the method, the cactus polysaccharide is extracted by a compound enzyme enzyme method mainly comprising natural plant enzymes, so that the cost is reduced, the extraction purity is improved, and the cactus polysaccharide can be efficiently extracted.

1. Name of the product of this design: Label (plant enzyme). 2. Purpose of this design product: used as a label affixed to a packaging bag. 3. The design key points of this appearance design product are the combination of shape, pattern and color. 4. The picture or photo that best illustrates the key points of the design: main view. 5. The design for which protection is sought includes color. 6. This design product is a thin product, and the rear view, top view, bottom view, left view and right view are omitted.

The present invention provides an astaxanthin-based antioxidant composition, a preparation method thereof, and an application thereof. The antioxidant composition comprises the following components in parts by weight: 0.02-1.0 parts of astaxanthin, 0.01-4 parts of a plant enzymatic hydrolysate, 0.01-3 parts of a plant fermentation product, and 0.01-1.5 parts of a grape skin extract. The present invention, by adding the plant enzymatic hydrolysate and the plant fermentation product, can synergistically improve the antioxidant properties of the antioxidant composition with astaxanthin; simultaneously, the addition of the plant enzymatic hydrolysate and the plant fermentation product can improve the stability of the antioxidant composition; and when astaxanthin, the plant enzymatic hydrolysate, and the plant fermentation product are mixed under specific ratios, the soothing effect of the antioxidant composition on the skin can be improved.

The present application relates to a kind of enzyme egg yolk liquid and its biological production method, belong to egg product technical field, using including compound plant component such as green pricklyash, Pleurotus eryngii as fermentation substrate, and using Lactobacillus bulgaricus and lactobacillus to ferment, preparation obtains compound plant enzyme, fermentation product can effectively inhibit the activity of oxidase, reduce the oxidative decomposition of lipid, maintain the quality and nutritional value of egg yolk liquid.Pretreated egg yolk liquid is mixed and is subjected to heat treatment, and enzyme egg yolk liquid is prepared.In the process of heat treatment, the protein in egg yolk liquid, including apo-protein, can be decomposed, the interaction between protein molecules is reduced, the formation of gel structure after egg yolk liquid freeze-thaw is reduced, and the bubble characteristics and taste are restored.

The invention provides a mild whitening composition and application thereof, and relates to the technical field of cosmetics. The nicotinamide, the tranexamic acid, the Grass rose extract, the bisabolol and the plant zymolyte are matched, the optimal compatibility dosage is given, all the components take effect in a synergistic mode, irritation of a traditional whitening component can be obviously reduced, and the whitening effect is improved. The invention also finds that the Grass rose extract, the bisabolol and the plant zymolyte are added into the whitening component, so that the whitening effect of the whitening component can be obviously improved.

The article of the design is a salad dressing enclosed in a packaging container. The size of the container is about 120 mm in length and 50 mm in width, and can hold an amount of dressing suitable for one person's salad. The dressing is a liquid made by extracting vegetable enzymes from onions, brewed vinegar, rapeseed oil (non-genetically modified), raw sugar, salt, garlic, fermented vegetable extract, etc., and is recommended to be stored refrigerated (at about 10°C or less). In order to maintain the state of the dressing for a long period of time, the packaging container is made of aluminum foil film using an extrusion laminate film with a biomass content of 20%. More specifically, this aluminum foil film is made by laminating PET12 / AL7 / PET12 / LLDPE40.

The application provides a full-value pet can for maintaining bone health and a preparation method, which comprises the following raw materials in mass parts: chicken breast meat 15-20 parts, chicken skeleton 3-7 parts, chicken heart 2-6 parts, beef 1-5 parts, beef liver 2-5 parts, tuna 5-10 parts, starch 9-14 parts, vegetables 17-23 parts, EPA 3-7 parts, vitamin K1-3 parts, folic acid 1-2 parts, nicotinic acid 0.5-1 part, mineral 2-6 parts, plant fermentation extract 5-9 parts, plant enzymolysis extract 3-6 parts and water 50-60 parts. The full-value pet can can provide full-value nutrition for pet cats, is beneficial to maintaining the bone health of the pet cats, prolongs the can storage time, inhibits the breeding of bacteria on the bottle opening and in the bottle after the can is opened, and improves the edible safety and storage time after the can is opened.

The invention discloses a high-efficiency green extraction method for active ingredients of sapindus mukorossi, and belongs to the technical field of plant extraction. According to the method, Chinese soapberry fruit peel is taken as a raw material, and active ingredients are extracted through a normal-temperature process comprising the steps of raw material pretreatment, enzyme synergistic primary extraction, hydrogen peroxide assisted dissolution extraction, sodium carbonate pH regulation secondary extraction and filtration detection, and the core is that cell walls of the fruit peel are destroyed by utilizing the synergistic effect of plant enzyme and hydrogen peroxide; and the solubility of the saponin is improved in combination with a weakly alkaline environment. High temperature is not needed, the retention rate of active ingredients reaches 92% or above, and the dissolution rate reaches 88% or above; only food-grade auxiliary agents are used, no chemical solvent is left, and filter residues are fully recycled; and compared with a high-temperature technology, the energy consumption is reduced by 65%, and the comprehensive cost is reduced by 20- The method fills the technical blank of'high efficiency-green-low cost 'extraction of the soapberry, is suitable for the fields of natural daily chemicals, medicines and the like, and has a remarkable industrialization value.

The present invention relates to a microbial agent for preventing and treating angelica root knot nematode disease and application thereof. The microbial agent comprises the following components: Bacillus velezine with a preservation number of CCTCC No: M2022923 ( Bacillus velezensis ), Bacillus mycoides with a deposit number of CCTCC No: M2022924 ( Bacillus Paramycoid ), Toosendan extract, amino oligosaccharides, fulvic acid and trace element fertilizers. The microbial agent can significantly improve root vitality, root enzyme activity and plant enzyme activity and proline content in the process of Angelica sinensis planting, improve crop resistance to nematodes; significantly increase soil pH, trace elements, enzyme activity and microbial diversity index, significantly reduce soil phenolic acid content, improve soil environment; and significantly reduce southern root-knot nematodes ( Meloidogyne incognita ) The number of second-instar larvae and the disease index can be reduced, and the nematode reduction rate can be increased. It has a significant effect on the prevention and control of southern root-knot nematode disease of Angelica sinensis and has broad application prospects.

The article of the design is a salad dressing enclosed in a packaging container. The size of the container is about 120 mm in length and 50 mm in width, and can hold an amount of dressing suitable for one person's salad. The dressing is a liquid made by extracting vegetable enzymes from onions, brewed vinegar, rapeseed oil (non-genetically modified), raw sugar, salt, garlic, fermented vegetable extract, etc., and is recommended to be refrigerated (stored at about 10°C or less). In order to maintain the state of the dressing for a long period of time, the packaging container is made of aluminum foil film using an extrusion laminate film with a biomass content of 20%. More specifically, this aluminum foil film is made by laminating PET12 / AL7 / PET12 / LLDPE40. The sales form of the article of the application can be placed in a transparent plastic bag with a leaflet introducing the types of dressing woven in, as shown in reference figures (1) and (2) showing the usage state.

The invention relates to the technical field of health care product preparation, in particular to fat dissolving powder and a preparation method thereof. Comprising the following raw materials in parts by weight: white kidney beans, flammulina velutipes, resistant dextrin, food essence, lactitol, inulin, vegetable and fruit plant enzyme powder, apple powder, compound probiotic powder, edible glucose, sorbitol, polydextrose, fructo-oligosaccharide, stachyose, xylooligosaccharide, L-arabinose, lactose and galactooligosaccharide. The fat dissolving powder disclosed by the invention selects the white kidney beans and the needle mushrooms as main materials, has the effects of promoting intestinal tract movement, accelerating the detoxification speed, helping a body to burn redundant fat, losing weight and beautifying, is rich in antioxidant substances, such as vitamin C and polyphenol compounds, and the components are beneficial to scavenging free radicals and reducing oxidative stress, so that the aging process is delayed, and the fat dissolving powder is suitable for being eaten by people. Various saccharides are added to further improve the intestinal environment, and L-arabinose is supplemented to inhibit the absorption of cane sugar and disaccharide in high-sugar and high-fat diet so as to achieve the effect of reducing blood sugar.

The invention belongs to the technical field of microorganisms, and particularly relates to a strain of Caribik Meyer yeast and application thereof. The strain is isolated from natural plant enzymes. In an in-vitro test, the strain has relatively high TG (Triglyceride) degradation capacity and T-CHO (T-CHO) degradation capacity; in an in-vivo test, the enzyme prepared by fermenting a seed solution of the strain can effectively reduce the weight, fat coefficient and blood fat level of obese mice and relieve pathological changes of fatty liver, has no obvious toxic or side effect, and can be used as a candidate for replacing weight-reducing medicines, so that the enzyme has good practical application value.

The invention discloses a bacteriostasis and deodorization composition, a preparation method thereof and a bacteriostasis and deodorization agent, the bacteriostasis and deodorization composition comprises a plant extract and a composite biological enzyme preparation, and the mass ratio of the plant extract to the composite biological enzyme preparation is 1: (0.1-0.5); the composite biological enzyme preparation comprises at least one plant enzyme and at least one animal enzyme, and the mass ratio of the plant enzyme to the animal enzyme is 1: (0.1-0.7). By implementing the cleaning agent, the effect of removing peculiar smell is achieved while cleaning and antibiosis are achieved, and no harm is caused to human beings and pets.

The invention provides a vegetable protein peptide special dietary nutrition product and a preparation method thereof, and relates to the technical field of food science, and the vegetable protein peptide special dietary nutrition product is prepared from the following raw materials: soybean protein isolate, inulin, polydextrose, resistant dextrin, soybean peptide powder, oat fiber powder, plant enzyme powder, hydroxymethyl cellulose, compound vitamins and compound minerals. The health-care food is rich in various amino acids, vitamins, mineral substances, dietary fibers and various nutritional ingredients necessary for human bodies, and can meet the nutritional requirements of special crowds; the vegetable protein peptide has the characteristics of small molecular weight and simple structure, and is easily digested and absorbed by a human body; through reasonable formula design and process optimization, the health-care food has good mouth feel and flavor.

The invention discloses mixed enzymolysis processing equipment compatible with animal and plant raw materials and a processing method. The mixed enzymolysis processing equipment comprises a main frame body; the plant protein enzymolysis assembly comprises a plant enzymolysis barrel, a first stirring rod arranged on the plant enzymolysis barrel and an enzyme feeding piece arranged on the plant enzymolysis barrel; the animal protein enzymolysis assembly comprises an animal enzymolysis barrel arranged beside the plant enzymolysis barrel, a second stirring rod arranged in the animal enzymolysis barrel and a decomposition part arranged on the animal enzymolysis barrel, and the animal enzymolysis barrel is connected with the plant enzymolysis barrel; the fusion assembly comprises a bearing cylinder connected with the plant enzymolysis cylinder and the animal enzymolysis cylinder, an acid-base adjusting part arranged in the bearing cylinder and a monitoring module arranged outside the bearing cylinder; the plant enzymolysis barrel, the animal enzymolysis barrel and the bearing barrel are arranged in the main frame body.

This invention relates to a whitening and stain-removing toothpaste and its preparation method, belonging to the field of oral care technology. The toothpaste contains 1.5-2.5 parts of plant enzymatic hydrolysate, 15-25 parts of a composite abrasive, and other components. The plant enzymatic hydrolysate is obtained by grinding walnut shell powder, beech, oyster shell, and Manegug glacial mud into powder in a mass ratio of 3:3:2:1, and then mixing and enzymatically hydrolyzing it with a composite enzyme solution. The composite abrasive and chelating agent are substances of specific particle sizes / molecular weights mixed in proportion. It also contains film-forming agents and enamel repair components. The preparation process includes the preparation of the plant enzymatic hydrolysate, the preparation of the mixture, and subsequent stirring, degassing, and filling steps. This toothpaste achieves highly effective whitening and stain removal, antibacterial properties, and long-lasting stain prevention, enamel repair, and antioxidant effects through the synergistic effect of multiple components, solving the problems of incomplete stain removal and enamel damage caused by traditional toothpastes.

The invention relates to the technical field of traditional Chinese medicine health-care drinks, in particular to spleen-tonifying and dampness-eliminating three-ginseng tea and a preparation method thereof.The three-ginseng tea is composed of American ginseng, codonopsis pilosula, radix pseudostellariae, astragalus membranaceus, radix ophiopogonis, corn stigma, poria cocos, phaseolus calcaratus, pericarpium citri reticulatae, lophatherum gracile and fresh lalang grass rhizome. According to the embodiment of the invention, the effects of American ginseng, codonopsis pilosula and other medicinal materials are combined, qi tonifying, yin nourishing and dampness clearing are considered, the nature is mild, and modern people with complex physique and daily nursing of sub-health people are adapted; low-temperature dynamic warm soaking at 60-75 DEG C is combined with compound plant enzyme extraction, so that heat-sensitive effective components are protected, the extraction rate is increased, and the biological activity of the product is guaranteed; the tea bag is prepared by stir-frying and stir-frying medicinal materials with honey and blending the taste to improve the taste, and then spray-drying and dry granulation, so that a traditional decoction is converted into standard granules, the stability and quality controllability of active ingredients are ensured, and the problems of easy yin injury, low dissolution rate of the active ingredients and poor taste due to imbalance of qi tonifying and dampness clearing in the prior art are finally solved.

1. Name of the product of this design: Packaging bottle (plant enzyme milk). 2. Purpose of this design product: This product is used for packaging. 3. The design highlights of this design product are: Design 1 is the combination of shape, pattern and color, and Design 2 is the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: Design 2 3D drawing. 5. Designate Design 2 as the base design. 6. Other circumstances that require explanation: Design 1 of this design requests protection of color.

The application discloses a kind of plant seed reinforced ground material and ground reinforcing method, belong to ground reinforcing technical field.The above-mentioned plant seed reinforced ground material includes plant enzyme supernatant and reaction liquid;Wherein, the plant enzyme supernatant is prepared by the following method: seed dry powder is immersed in leaching solution, stirring forms suspension after being placed, and filtrate and supernatant I are obtained by filtration;The obtained residue is immersed in leaching solution again, stirred and placed, and supernatant II is collected by filtration, and supernatant I and supernatant II are uniformly mixed to be prepared;Reaction liquid is urea and calcium chloride mixed solution.In the application, raw materials used are less, and the raw materials involved do not contain acetone and other chemical solvents, and filter cloth and other consumables and by-products such as residue can be recycled, which realizes true renewable and pollution-free in raw material use;The preparation process is simple, and centrifugation and other plant enzyme extraction processes are not required, which can save a lot of manpower and financial resources, and is helpful to large-scale application of EICP technology.

The invention discloses a non-instant multipurpose seasoning, and relates to the technical field of seasonings. Comprising the following raw materials in parts by weight: 9-17 parts of acer truncatum seeds, 7-14 parts of burdock roots, 4-8 parts of astragalus membranaceus, 2-9 parts of mulberry leaves, 2-8 parts of osmanthus fragrans, 2-8 parts of chrysanthemum, 4-9 parts of dandelion, 2-6 parts of mint leaves, 4-10 parts of purslane, 4-8 parts of mulberries, 4-9 parts of momordica grosvenori, 3-7 parts of bitter apricot kernels, 2-5 parts of Chinese yams, 4-7 parts of spina date seeds, 1-5 parts of radix angelicae, 2-6 parts of ginkgo and 1-6 parts of cassia seeds. The plant enzyme seasoning can be used for replacing various seasonings and improving the taste, has the effects of reducing the original flavor and nutrition of food, can degrade various toxins, chemical fertilizer and pesticide residues, purine, harmful heavy metals and the like in drinking water and food materials, is free of tainting of odor, can purify intestinal tracts, enhance metabolism, eliminate dampness and cold and eliminate damp toxin and cold toxin, and has the effects of improving the health of people and improving the health of people. And the gastrointestinal, skin and physical states can be improved.

Drought is one of the biggest problems in the world. Drought in agricultural production soils also increases the salinity of the soil. The plant is more than one abiotic in natüre encounters stress. In this process, if the plant reacts to the antioxidant enzymes and survives, it is called a resistant plant. The enzyme response is insufficient and the increase in the level of TBARS causes plant deaths. This invention organic fertilizer strengthens the plant antioxidant enzyme and protects the plant against drought and salt stress.

The invention belongs to the technical field of enzyme activity detection, and particularly relates to a method for detecting plant ALKBH enzyme activity and application. The method for detecting the enzyme activity of the plant ALKBH comprises the following steps: expressing and purifying by using an expression system to obtain a recombinant ALKBH protein; incubating the recombinant ALKBH protein and a nucleic acid molecule substrate, and then carrying out enzyme digestion to obtain an enzyme digestion product; and carrying out electrophoresis migration and nucleic acid protein interaction analysis on the enzyme digestion product, and representing the plant ALKBH enzyme activity according to the number and definition of bands of an image. The method disclosed by the invention is high in safety and simple to operate, can effectively detect the activity of the ALKBH enzyme in the plant, and can be used as a plant ALKBH enzyme activity detection method which is wide in application range, high in practicability and high in stability.

The invention relates to the technical field of cotton planting, in particular to a liquid fertilizer for cotton planting in high-salt soil and a preparation method of the liquid fertilizer, and the liquid fertilizer is prepared from the following components in percentage by volume: 35-40% of nitrohumic acid solution, 25-30% of nitrohumic acid potassium solution, 15-20% of compound microbial agent, 10-15% of plant enzyme and 3-5% of synergist. Wherein the compound microbial agent comprises one or more of bacillus subtilis, bacillus mucilaginosus and saccharomyces cerevisiae. According to the method, a nitrohumic acid-bio-organic fertilizer-complex microbial inoculant compound fertilizer system is created for the first time, the salt content of the high-salinity land is reduced in a stepped mode within 1-2 years, the planting environment of the high-salinity land is restored, and cotton planting is facilitated.