The invention discloses a construction and identification method of super-
obese mice. The super-
obese mice comprise male PPAR alpha- / -mice, female OB / OB mice, a PCR (
polymerase chain reaction) amplification instrument,
a DNA (deoxyribonucleic acid) amplification product, 2%
agarose gel electrophoresis, an identification basis and a
leptin gene, wherein a PCR kit is arranged in the PCR amplification instrument; raw materials are arranged in the PCR kit; the raw materials comprise
DNA double strands,
DNA single strands, primers, TapDNA
polymerase, adenine deoxynucleotide,
guanine deoxynucleotide,
cytosine deoxynucleotide and
thymine deoxynucleotide; and the identification basis comprises white adipose tissues, brown adipose tissues,
triglyceride,
cholesterol, liver,
fatty liver, glucose andinsulin. The invention also discloses an identification method of the super-
obese mice. The construction and identification method has the
advantage that the PPAR alpha
delta OB / OB super-obese mice provide a more ideal model for researching
obesity and metabolic syndromes thereof and researching the effect of a PPAR alpha
signal channel in the
obesity occurrence and development process.