Preparation method and application of cordyceps active oligosaccharide

A technology of Cordyceps and oligosaccharides, which is applied in the preparation of sugar derivatives, organic active ingredients, chemical instruments and methods, etc., can solve the problems of difficult mass production, and achieve the effects of less side reactions, high yield, and no waste liquid

Inactive Publication Date: 2015-10-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, chemical synthesis can synthesize oligosaccharides. Due to the multi-step synthesis strategies involved in the selection of glycosidation reagents, intermediate product synthesis, group protection, and removal of by-products, it is only suitable for small quantities of g-grade products in the laboratory, and it is difficult to prepare in large quantities.

Method used

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  • Preparation method and application of cordyceps active oligosaccharide
  • Preparation method and application of cordyceps active oligosaccharide
  • Preparation method and application of cordyceps active oligosaccharide

Examples

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Effect test

Embodiment 1

[0038] The preparation method of embodiment 1 class cordyceps active oligosaccharides, the implementation steps are as follows:

[0039] Mix glucose, galactose, mannose, and rhamnose monomer mass ratio 1.5︰10︰4︰0.3 mixture (85%), phosphoric acid (1.5%), deionized water (13.5%), and mix well, and the mixed materials enter Groove belt microwave reactor, microwave frequency 2.45MHz, microwave power 950w, temperature 110-115°C, condensation reaction for 8 minutes to form Cordyceps-like oligosaccharides, soaked in cold ethanol, precipitated and dried, and pulverized to obtain Cordyceps-like active oligosaccharides powder .

Embodiment 2

[0040] The preparation method of embodiment 2 class cordyceps active oligosaccharides, the implementation steps are as follows:

[0041] Mix glucose, galactose, mannose, rhamnose monomer mass ratio 1.5︰2.5︰4.8︰0.5 mixture (89%) with citric acid (2.5%), deionized water (8.5%), mix evenly, and put the material into the concave Slot-belt microwave reactor, microwave frequency 2.45MHz, microwave power 1000w, temperature 120-128°C, condensation reaction for 7 minutes to form Cordyceps-like oligosaccharides, soaked in cold ethanol, precipitated and dried, and pulverized to obtain Cordyceps-like active oligosaccharides powder.

Embodiment 3

[0042] Example 3 The preparation method of Cordyceps-like active oligosaccharides, the implementation steps are as follows:

[0043] Glucose, galactose, mannose, rhamnose monomer mass ratio 1.5︰4︰5︰0.2 mixture (88%) hydrochloric acid (1.5%), deionized water (10.5%) are mixed evenly, the mixed material is melted, Put into a grooved belt microwave reactor, microwave frequency 2.45MHz, microwave power 2000w, temperature 120-140°C, condensation reaction 8.5min, form Cordyceps-like oligosaccharides, soak in cold ethanol, precipitate and dry, and pulverize to obtain Cordyceps-like activity Oligosaccharide powder.

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Abstract

The invention provides a preparation method and application of cordyceps active oligosaccharide, and belongs to the technical field of functional dietary fiber preparation. According to the preparation method, the cordyceps active oligosaccharide is composed of the components of glucose, galactose, mannose and rhamnose according to the weight ratio of 1-10:2-10:1-20:0.2-2; the glucose, the galactose, the mannose and the rhamnose are raw material carbohydrate and are formed through compound reaction of monosaccaride under the action of an electromagnetic field and an acid catalyst, and the bond types of the glucose, the galactose, the mannose and the rhamnose are main 1-3, 1-6 and 1-2. The preparation method has the advantages of being fast in synthetic speed, free of waste liquid, less in side reaction, friendly to environment and high in yield. The molecular weight of the cordyceps active oligosaccharide reaches 0.7-4 kDa, and the polymerization degree of cordyceps active oligosaccharide reaches 4-22. Biological experiments prove that the cordyceps active oligosaccharide has the better vitro antioxidant property and can promote probiotics to proliferate; after being taken orally, the cordyceps active oligosaccharide can lower blood lipid of obese mice, regulate intestinal flora and boost immunity, and has a certain anti-obesity effect.

Description

technical field [0001] A preparation method and application of a cordyceps-like active oligosaccharide belongs to the technical field of preparation of functional dietary fiber. Background technique [0002] Cordyceps is a fungus belonging to the Ergotaceae family, with a wide distribution area, unique life history, and diverse species, including cicadae, Cordyceps militaris, Cordyceps macrocystis, Cordyceps Kaohsiung, etc. So far, more than 400 species of Cordyceps have been discovered. Cordyceps polysaccharide is the One of the main pharmacologically active ingredients with multiple biological activities: [0003] 1. Anti-tumor activity: Intracellular and extracellular polysaccharides of Cordyceps can stimulate non-specific defense system, inhibit tumor cell growth, reduce micronucleus rate, and indirectly kill cancer cells through immune regulation. Ascites tumor etc. have inhibitory effect. [0004] 2. Immunomodulatory effect: promote the proliferation and differentiat...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H3/06C07H1/00A61K31/715A61K31/702A61P1/00A61P37/04A61P3/06A61P39/00
Inventor 施用晖乐国伟王海松沈弘
Owner JIANGNAN UNIV
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