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Novel bifidobacterium longum or lactobacillus rhamnosus strain having effect of preventing or treating obesity, and use thereof

A technology of Bifidobacterium longum and strains, which is applied in the field of Bifidobacterium longum strains or Lactobacillus rhamnosus strains, can solve the problem of undisclosed Lactobacillus rhamnosus strains, etc., so as to improve lipid metabolism, prevent or treat obesity Effect

Pending Publication Date: 2021-04-16
KOREA RES INST OF BIOSCI & BIOTECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned documents do not disclose "a Bifidobacterium longum strain or a Lactobacillus rhamnosus strain having an effect of preventing or treating obesity by inducing the formation of beige adipocytes and brown adipocytes and uses thereof" according to the present disclosure.

Method used

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  • Novel bifidobacterium longum or lactobacillus rhamnosus strain having effect of preventing or treating obesity, and use thereof
  • Novel bifidobacterium longum or lactobacillus rhamnosus strain having effect of preventing or treating obesity, and use thereof
  • Novel bifidobacterium longum or lactobacillus rhamnosus strain having effect of preventing or treating obesity, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1. Search for anti-obesity active bacterial strains

[0103] To observe the possibility of using 55 Lactobacillus strains to transform into beige and brown adipocytes in 3T3-L1 preadipocytes, each Lactobacillus strain was cultured in MRS medium for 48 hours, and then centrifuged to obtain the supernatant. The resulting supernatant was freeze-dried, and then 1 / 10 of the initial volume of sterile distilled water was added to the supernatant to prepare a concentrate thereof. 1, 5 and 10 μl of each of this concentrate were applied to 3T3-L1 cells, in which the accumulated amount of triglyceride was quantified by Oil Red O staining. Therefore, active candidate groups (bars filled with diagonal lines) that cause browning and beige adipocytes were selected in the lactic acid bacteria medium. In addition, candidate groups (bars filled with dots) were selected from which suppressed adipocyte formation (Fig. 1B).

[0104] In the group in which the proliferation of adi...

Embodiment 2

[0106] Embodiment 2. Effect of lactic acid bacteria medium on mouse brown adipocyte and beige adipocyte-specific gene expression influences

[0107] Beige adipocytes can express the UCP1 (uncoupling protein 1) gene that is not expressed in white adipose tissue. Expression of this gene in white adipocytes is known to indicate that transdifferentiation from white adipocytes to beige adipocytes or brown adipocytes has occurred, and the transdifferentiation of cells occurs reversibly depending on feeding or external environment. Therefore, in this experiment, the effect of culture media of selected strains on the expression of genes specific to beige adipocytes and brown adipocytes was investigated.

[0108]The effect of lactic acid bacteria medium on mouse brown and beige adipocyte-specific gene expression was studied. As shown in Figure 3A, in 3T3-L1 adipocytes treated with #30 lactic acid bacteria medium, the expression of the uncoupling protein 1 (UCP1) gene, which is cal...

Embodiment 3

[0112] Embodiment 3. Lactic acid bacteria medium is to the expression of fat degradation and β-oxidation related genes and to PKA signal transduction The effect of the activation of the process

[0113] In order to further identify the effect of the lactic acid bacteria medium according to the present disclosure, the lactic acid bacteria medium was applied to 3T3-L1 cells, and then the expression levels of genes Atgl, HSL, Plin1, and Plin5 related to lipolysis were identified. The expression levels of the genes LCAD, MCAD, LCPT and Abhd5 genes related to the β-oxidation of lipids were identified.

[0114] As a result, in 3T3-L1 cells treated with lactic acid bacteria medium #30 and #51 according to the present disclosure, the expression levels of Atgl, HSL, Plin1, Plin5 genes and LCAD, MCAD, LCPT and Abhd5 genes were higher than the control. (FIGS. 5A and 5B). Therefore, it was confirmed that the treatment has the effect of suppressing fat accumulation and weight gain by ...

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Abstract

The present invention relates to a novel Bifidobacterium longum or Lactobacillus rhamnosus strain having an effect of preventing or treating obesity, and a use thereof. Treatment of each of the lactic acid bacteria strains of Bifidobacterium longum and Lactobacillus rhamnosus, of the present invention, causes browning of white adipocytes and, particularly, it has been confirmed that beige adipocyte- and brown adipocyte-specific gene expression significantly increases over that of an untreated control group. In addition, as a result of experimentation on high fat diet-induced obese mice is carried out, each strain exhibits a weight gain inhibitory effect more significant than that of a negative control group, and an effect of increased expression of a thermogenesis-specific gene, in the white adipocytes of the mice, has been confirmed. Therefore, each of the lactic acid bacteria strains of Bifidobacterium longum and Lactobacillus rhamnosus exhibits an anti-obesity effect so as to be effectively usable as a food, a drug or a feed for preventing or treating obesity, thereby being very useful in related industries.

Description

【Technical field】 [0001] The present disclosure relates to novel Bifidobacterium longum strains or Lactobacillus rhamnosus strains having obesity prevention or treatment effects and uses thereof. 【Background technique】 [0002] With the modernization of the economy in recent years and the improvement of living standards, the intake of fat and sugar has increased, and the intake of fiber has decreased. Especially due to the establishment of the culture of eating out, the phenomenon of preference for high-protein diets is remarkable. Instead, the reality is that the obese population is rapidly increasing due to the lifestyle of modern people with little physical activity. Obesity, especially abdominal obesity, is becoming a serious social problem as the intake of high-calorie foods increases and fiber intake decreases. Obesity refers to a state in which adipose tissue is excessively increased, and weight gain due to obesity is mainly due to increased fat. The mechanism by wh...

Claims

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Application Information

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IPC IPC(8): A61K35/744A61K35/745A61K35/747A23L33/135A23K10/16A61P3/04
CPCA61K35/744A61K35/745A23K10/16A61K35/747A23L33/135A61P3/04A23V2002/00A23V2200/332
Inventor 金容植朴斗常孙美英奇秀真金志宣吴贤宇李婑璃全晙亨郑先娥
Owner KOREA RES INST OF BIOSCI & BIOTECH
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