10 results about "Brown Adipocytes" patented technology

To provide non-naturally occurring three-dimensional (3D) brown adipose-derived stem cell aggregates, and methods of making and using the same.SOLUTION: The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates. Also provided herein is an encapsulation system comprising non-naturally occurring 3D brown adipose-derived stem cell aggregates. The 3D brown adipose-derived stem cell aggregate may comprise brown adipose-derived stem cells that express one or more brown adipocyte gene in the absence of differentiation medium.SELECTED DRAWING: None

The invention relates to a compound for improving heat production and metabolic disorder of middle-aged and elderly people or obese individuals and application of the compound, in particular to application of betaine or an accelerant of the betaine, and particularly discloses application of the betaine or the accelerant of the betaine and application of the betaine or the accelerant of the betaine to preparation of a preparation or a pharmaceutical composition, and the preparation or the pharmaceutical composition is used for (a) adjusting heat production; and / or (b) ameliorating metabolic disorders. The betaine or the accelerant thereof can promote up-regulation of expression of heat-producing genes and / or expression of UCP1 in brown adipocytes, so that the body temperature is increased.

The invention discloses application of vitexin in preparation of medicines for preventing and treating obesity. According to the application of the vitexin in preparation of the medicine for preventing and treating the obesity, the prevention and treatment comprise one or more of prevention, relieving or treatment of the obesity induced by high fat diet, and the medicine can be generally a solution and contains pharmaceutically acceptable auxiliary materials. The vitexin disclosed by the invention can promote the heat production of brown adipocytes and increase the oxygen consumption; the expression of heat production marker protein UCP1 of brown fat and subcutaneous white fat tissues is increased, the metabolic rate of an organism is increased, obesity induced by high fat diet is resisted, and then insulin resistance is improved.

The present invention provides a composition having a metabolism-improving action, which comprises a supernatant of brown adipocytes or a purified product thereof. The present invention also provides a method of preparing the supernatant without using a culture solution comprising a high concentration of glucose. The present invention also provides a method of producing brown adipocytes using pluripotent stem cells, which are useful for preparing the supernatant of brown adipocytes. The present invention has succeeded in obtaining a supernatant having a metabolism-improving action from brown adipocytes. It was also possible to obtain the supernatant without using a culture solution comprising a high concentration of glucose. The present invention has also succeeded in producing brown adipocytes from pluripotent stem cells using a feeder-free culture system without adding a cytokine cocktail or the like. The brown adipocyte supernatant of the present invention is expected to be applied to the development of therapeutic agents and cosmetic products for glucose metabolism diseases.

An object of the present invention is to provide an anti-obesity composition and an oral composition that can promote the gene expression of proteins such as PGC1α and UCP1 related to brown adipocytes or their energy-consuming effects, and have the effect of promoting energy-consuming effects in brown adipocytes. The composition of the present invention contains irigenin and pinitol. The irigenin is preferably irigenin. The composition of the present invention is preferably used as an anti-obesity composition and an oral composition.

The compounds and methods of the present disclosure exhibit induce Ucp1 transcription, enhance of mitochondrial respiration, activate protein kinase A, increase lipolysis, and increase p38 MAPK phosphorylation in cells, particularly brown adipocytes and white adipocytes. They also protect primary cardiomyocytes against hypertrophy induced by adrenergic agonists. Such compounds and methods are useful in the treatment and prevention of conditions such as obesity and associated complex metabolic, endocrine, and hemodynamic changes, as well as related conditions as dyslipidemias, cardiovascular disease, and type 2 diabetes.

The invention discloses a composition for promoting browning of white fat cells and a preparation method thereof, and belongs to the technical field of biological medicine, the composition is prepared from the following raw materials by weight: 5-8 parts of 2-BrO, 2-5 parts of anacaric acid, 1-2 parts of 8-cyclopentyl-1, 3-dipropyl xanthine (DPCPX), 1-2 parts of endothelin-31, 0.1-0.5 part of S-4-nitrobenzyl-6-thioinosine (NBTI), and 1-2 parts of catecholamine. The liposome of which the surface is modified by the CKGGRAKDC is used as a carrier for targeting white fat cells, and the carrier for targeting the white fat cells is coated with 2-BrO, anacardic acid, S-4-nitrobenzyl-6-thioinosine, endothelin-3 and catecholamine. The finally prepared composition for promoting browning of white adipocytes promotes fat in adipose tissues to be decomposed into fatty acid, enhances the beta oxidation effect of the fatty acid, improves heat production efficiency, maintains normal reactivity of fat decomposition, and effectively promotes the white adipocytes to be browned into brown adipocytes.

It is an object of the present invention to provide an anti-obesity composition and an oral administration composition that can promote the gene expression of proteins such as PGC1α and UCP1 that are involved in brown adipocytes and the energy consumption activity of the brown adipocytes, and have the effect of promoting the energy consumption activity in the brown adipocytes. The compositions of the present invention contain a tectorigenin-like substance and pinitol. The tectorigenin-like substance is preferably tectorigenin. The compositions of the present invention can suitably be used as an anti-obesity composition or an oral administration composition.

Provided herein are methods of generating Gata6-positive brown adipose progenitor cells, and brown adipose cells and tissue therefrom. Additionally, provided herein are methods of generating UCP1 positive organoids. Generally, the methods include a step of culturing brown adipose progenitor cells in a medium containing an FGF signaling pathway activator, a BMP signaling pathway activator, a TGFβ activator, a Wnt inhibitor, and a thyroid hormone receptor activator.