The invention relates to the field of plant extracts, in particular to application of a flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care. The flammulina velutipes extract provided by the invention is used for regulating the expression quantities of an XRCC1 gene, a UNG gene, an OGG1 gene, an MPG gene, an ERCC1 gene, an ERCC6 gene, a GPX1 gene, an MLH1 gene, an MSH6 gene, an SOD gene, an MKP-1 gene and a DDC gene, improving the anti-glycation activity, inhibiting cell fat accumulation, promoting lipolysis, improving the skeletal muscle basal metabolic rate, resisting depression, inhibiting melanogenesis, improving the anti-ultraviolet ability of skin, improving SOD activity, reducing ROS expression quantity, improving granulocyte activity and improving oxidation resistance. The flammulina velutipes extract is obtained by extracting flammulina velutipes with a solvent, and the solvent is water, alcohol or an alcohol-water mixture.