Composition for improving basic metabolism during low-carbon diet

Abstract

The invention discloses a composition for improving basic metabolism during low-carbon diet, and belongs to the technical field of food. The composition is prepared from the following components in parts by weight: 0.01 to 0.5 part of opuntia ficus-indica juice powder, 0.01 to 0.5 part of guarana extract, 10 to 50 parts of virgin coconut oil microcapsule powder, 1 to 10 parts of medium chain triglyceride microcapsule powder, 0.1 to 1 part of grape seed extract, 0.01 to 0.1 part of potato extract and 0.1 to 1 part of pomegranate extract. The composition can effectively improve the basal metabolic rate during a low-carbon diet period at a lower dosage. According to the innovative combination mode, the basal metabolic rate is increased, the problem of insufficient energy possibly occurring in the low-carbon diet period is relieved, and side effects and cost increase possibly caused by addition of high-dose plant extracts are avoided.

Description

Technical Field

[0001] The invention belongs to the field of food technology, and in particular relates to a composition for improving basal metabolism during a low-carb diet. Background Art

[0002] In the fields of nutrition, food science and metabolic regulation, low-carb diet has received widespread attention as a popular diet. Low-carb diet mainly promotes fat burning and utilization by limiting carbohydrate intake and putting the body into a ketogenic state. However, there are also some problems with low-carb diet, such as a decrease in basal metabolic rate and reduced energy consumption, which may lead to stagnation or rebound in weight loss. Therefore, how to improve basal metabolism during a low-carb diet has become a hot topic in research in this field.

[0003] Solutions of existing technologies: Among existing technologies, there are some solutions to increase the basal metabolic rate by adding specific nutrients. For example, adding ingredients such as caffeine and capsaicin can promote metabolism and increase the basal metabolic rate. In addition, there are some solutions to increase the basal metabolic rate by adding specific plant extracts. For example, adding prickly pear cactus extract, guarana extract, etc. can promote fat decomposition and increase the basal metabolic rate.

[0004] Problems with existing technologies: Although there are some solutions in existing technologies that can increase the basal metabolic rate, the effects of these solutions are often limited and may have some side effects. For example, adding ingredients such as caffeine and capsaicin may cause insomnia, stomach discomfort and other problems. In addition, existing plant extract solutions often require higher doses to achieve significant effects, which may increase costs and may also increase the burden on organs such as the liver. Therefore, how to effectively increase the basal metabolic rate by adding lower doses during a low-carb diet has become an urgent problem to be solved in this field. Summary of the invention

[0005] One of the purposes of the present invention is to provide a composition for improving basal metabolism during a low-carb diet, the composition comprising the following components in parts by weight: 0.01-0.5 parts of prickly pear cactus juice powder, 0.01-0.5 parts of guarana extract, 10-50 parts of virgin coconut oil microcapsule powder, 1-10 parts of medium-chain triglyceride microcapsule powder, 0.1-1 parts of grape seed extract, 0.01-0.1 parts of potato extract and 0.1-1 parts of pomegranate extract.

[0006] Preferably, the composition comprises the following components in parts by weight: 0.05 parts of prickly pear cactus juice powder, 0.05 parts of guarana extract, 30 parts of virgin coconut oil microcapsule powder, 3 parts of medium chain triglyceride microcapsule powder, 0.5 parts of grape seed extract, 0.05 parts of potato extract and 0.5 parts of pomegranate extract.

[0007] The second object of the present invention is to provide a method for preparing the above-mentioned composition, which comprises the following steps:

[0008] (1) preparing prickly pear cactus juice powder, guarana extract, virgin coconut oil microcapsule powder, medium chain triglyceride microcapsule powder, grape seed extract, potato extract, and pomegranate extract;

[0009] (2) Mix all the components evenly and make the mixture into powder.

[0010] Preferably, during the mixing process in step (2), a stirrer is used for mixing.

[0011] More preferably, in step (2), the stirring speed of the stirrer is 500 rpm and the stirring time is 10 minutes.

[0012] More preferably, the particle size of the powder in step (2) is 10 microns.

[0013] More preferably, the method further comprises step (3) packaging.

[0014] The third object of the present invention is to provide the use of the above composition in the preparation of products for improving basal metabolism during a low-carb diet.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The plant extract composition of the present invention can effectively improve the basal metabolic rate during a low-carb diet at a relatively low dose by scientifically mixing a variety of plant extracts such as prickly pear cactus juice powder, guarana extract, virgin coconut oil microcapsule powder, medium-chain triglyceride microcapsule powder, grape seed extract, potato extract, and pomegranate extract. This innovative combination not only improves the basal metabolic rate, but also avoids the side effects and cost increase that may be caused by adding high doses of plant extracts.

[0017] (2) The plant extract composition of the present invention, wherein the virgin coconut oil microcapsule powder and the medium-chain triglyceride microcapsule powder contained therein, can provide medium-chain fatty acids, which can be quickly decomposed and utilized to provide energy, while promoting the decomposition and utilization of fat, further improving the basal metabolic rate. This innovative ingredient can not only improve the basal metabolic rate, but also provide energy, alleviating the energy deficiency problem that may occur during a low-carb diet.

[0018] (3) The plant extract composition of the present invention, including grape seed extract and potato extract, can provide antioxidants and anti-inflammatory substances, which can help reduce oxidative stress and inflammatory reactions that may occur during a low-carb diet, protect organs such as the liver, and improve the overall health of the body. This innovative ingredient can not only increase the basal metabolic rate, but also protect the overall health of the body.

[0019] (4) The plant extract composition of the present invention has a simple preparation process, is easy to implement, and is suitable for large-scale production and application. This innovative preparation process not only improves production efficiency but also reduces production costs, making the plant extract composition easier to promote and apply.

[0020] Due to the advanced nature of this technical solution, it can be widely used in application fields such as nutrition, food science, and metabolic regulation. First, in the field of nutrition, this technical solution provides a plant extract composition that improves basal metabolism during a low-carbon diet. By adding a lower dose, the basal metabolic rate can be effectively improved, which will help solve the problem that a higher dose needs to be added in the prior art to achieve a significant effect, thereby reducing costs and reducing the burden on organs such as the liver. In addition, since the plant extracts added in this technical solution are all natural extracts and have no side effects, this will give it broad application prospects in the field of nutritional products and health foods. Secondly, in the field of food science, this technical solution provides a new type of food additive. By adding the plant extract composition of this technical solution, the metabolic function of food can be improved and the utilization efficiency of food by the human body can be improved. This will help improve the taste and nutritional value of food and meet consumers' demand for high-quality food, thus having broad application prospects in the food industry. Finally, in the field of metabolic regulation, this technical solution provides a new metabolic regulation method. By adding the plant extract composition of this technical solution, the basal metabolic rate during a low-carbon diet can be effectively improved, which will help solve the problem of decreased basal metabolic rate and reduced body energy consumption in a low-carbon diet, thus having broad application prospects in the field of metabolic regulation. In general, as people's awareness of healthy diet and nutritional needs continues to increase, this technical solution has broad application prospects and market demand in application fields such as nutrition, food science, and metabolic regulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is the resting metabolic rate of each group of zebrafish in Example 2.

[0022] Figure 2 This is a typical graph of the fluorescence signal intensity of zebrafish yolk sac fat after treatment in each group in Example 3.

[0023] Figure 3 This is a quantitative statistical graph of the fluorescence intensity of Nile red staining of zebrafish yolk sac fat after treatment in each group in Example 3. DETAILED DESCRIPTION

[0024] The pear cactus juice powder used in the following examples is produced by Tianjin Binhai Jiecheng Special Chemical Co., Ltd., guarana extract is produced by Hangzhou Tiancao Technology Co., Ltd., virgin coconut oil microcapsule powder is produced by Qingdao Haizhiyuan Life Science Co., Ltd., medium-chain triglyceride microcapsule powder and grape seed extract are produced by Dalian Yinuo Biological Co., Ltd., potato extract is produced by Shanghai Chifeng Biotechnology Co., Ltd., and pomegranate extract is produced by Shanghai Nuoshen Food Trading Co., Ltd.

[0025] Example 1

[0026] This embodiment provides a composition for improving basal metabolism during a low-carb diet, and the specific steps are as follows:

[0027] Step 1: Prepare prickly pear cactus juice powder and guarana extract, adding them in amounts of 0.05 parts and 0.05 parts respectively.

[0028] Step 2: Prepare virgin coconut oil microcapsule powder and medium chain triglyceride microcapsule powder, adding 30 parts and 3 parts respectively.

[0029] Step 3: Prepare grape seed extract, potato extract, and pomegranate extract, and add them in amounts of 0.5 parts, 0.05 parts, and 0.5 parts respectively.

[0030] Step 4: Mix all the extracts and microcapsule powder evenly. During the mixing process, use a blender at a speed of 500 rpm for 10 minutes.

[0031] Step 5: Powder the mixture using a spray dryer, and the mixture is powdered with a powder particle size of 10 microns.

[0032] Step 6: Packaging: The powdered composition is packaged into small bags, each bag having a net content of 10 grams.

[0033] The plant extract composition in this embodiment, by adding an appropriate amount of pear cactus juice powder and guarana extract, can effectively improve the basal metabolism during the low-carbon diet, and avoid the problem of stagnation or rebound of weight loss. At the same time, by adding virgin coconut oil microcapsule powder and medium-chain triglyceride microcapsule powder, energy and essential fatty acids can be provided to the body without adding too much calories. In addition, the addition of grape seed extract, potato extract and pomegranate extract can protect organs such as the liver and reduce the burden on organs such as the liver. Finally, by combining all extracts and microcapsule powders, a synergistic effect can be exerted to improve the overall effect.

[0034] Example 2 Zebrafish resting metabolic rate determination

[0035] As a new model organism, zebrafish is widely used in the efficacy research of drugs and functional foods. Its genes are 87% similar to humans, and it is similar to mammals in energy metabolism and oxidative stress response, which indicates that zebrafish and humans may have common mechanisms in certain metabolic pathways. Therefore, the present technology uses zebrafish to detect the effect of the patent composition on the basal metabolic rate (resting metabolic rate). Referring to the self-made flow-type respirometer method of Professor Luo Yiping's laboratory at Southwest University (Li Y, Lv X, Zhou J, Shi C, Duan T, Luo Y. Does air-breathing meet metabolic demands of the juvenile snakehead, Channa argus, in multiple conditions. Biol Open. 2017 May 15; 6 (5): 642-647.), the self-made respirator measures the oxygen consumption rate of zebrafish, and the average value of the oxygen consumption rate is used as the resting metabolic rate value. The respirometer measurement includes a large water tank, 15 closed respiration chambers, a water pump system, a temperature control system, a switch that can adjust the flow rate, and a sensitive fiber optic sensor dissolved oxygen monitoring system. The specific steps are as follows:

[0036] (1) The experiment set up a blank control group, Example 1 group and Comparative Example 1 group, selected 5-month-old AB strain zebrafish, 10 zebrafish in each group, and placed the zebrafish in pure water containing 3 mg / mL of the composition of Example 1 and 3 mg / mL of the composition of Comparative Example 1, respectively. The blank control group was pure water, and fresh water containing the corresponding combination was replaced every 24 hours. After 72 hours of treatment, 4 fish were randomly selected from the three groups for the experiment.

[0037] Table 1

[0038]

[0039] (2) Before the experiment, zebrafish were placed in a respiration chamber (8 mL) to acclimate for 12 h. To maintain a constant temperature, the respiration chamber should be immersed in a water tank, and a heating rod with a temperature control switch should be used to keep the water temperature of the entire water system constant at 28°C, and the room temperature should be regulated at 28°C by air conditioning.

[0040] (3) Before measurement, calculate the flow rate by timing the time it takes to fill up a 10 mL volumetric flask, and adjust the valves to make the flow rates in each breathing chamber similar.

[0041] (4) A respiration chamber without fish was used as a control to eliminate interference from other oxygen consumption factors. The oxygen consumption of the fish was calculated by detecting the flow rate and dissolved oxygen at the outlet.

[0042] The calculation formula is: oxygen consumption rate (MO2) = ΔO2 × v / m

[0043] Where ΔO2(mg O2L -1 ) is the difference in dissolved oxygen between the blank control respiratory chamber and the experimental group respiratory chamber; v(lh -1 ) is the flow rate of water passing through the respiration chamber; m(g) is the weight of the experimental fish. The oxygen consumption rate was measured every 3 h, and after 8 measurements in 24 h, the average value was taken as the resting metabolic rate value.

[0044] like Figure 1 The results showed that compared with the blank control group, the resting metabolic rate of the zebrafish fed with Example 1 was significantly increased (p < 0.05), while the resting metabolic rate of the zebrafish fed with Comparative Example 1 was not significantly changed compared with the blank control group (p > 0.05).

[0045] Example 3 Zebrafish model for promoting lipolysis

[0046] The lipid composition of zebrafish yolk sac (mainly triglycerides and lecithin) is similar to the abdominal fat of mammals / humans (white fat, about 95% of which is triglycerides). If the absorption of zebrafish yolk sac can be promoted, it is equivalent to promoting fat decomposition and metabolism, similar to fat burning in the human body. Therefore, this technology uses zebrafish fry to evaluate the effect of the patented composition in promoting decomposition. The specific steps are as follows:

[0047] (1) The experiment used AB zebrafish, and the zebrafish embryos were bred by natural pair mating. Phenylthiourea solution was added to 1dpf zebrafish embryos to remove most of the melanin, and the zebrafish fry on the second day after fertilization (2dpf) were used to evaluate the maximum tolerated concentration of each composition to promote lipolysis and its efficacy evaluation;

[0048] (2) Normally developed zebrafish at 2 dpf were randomly selected and placed in a six-well culture plate, with 15 zebrafish in each well. The standard dilution water in the six-well plate was removed without harming the embryos, and 3 mL of 5 mg / mL, 2.5 mg / mL, 1.25 mg / mL, 0.625 mg / mL, 0.3125 mg / mL, and 0.1562 mg / mL dilutions of the composition of Example 1 were quickly added to each well. The culture plate panel was covered and wrapped with aluminum foil, and incubated in a biochemical incubator at (28.5±1)°C in the dark. After 2 days, the maximum tolerance concentration (MTC) of the sample for zebrafish was determined. The results showed that fry died at 5 mg / mL, 2.5 mg / mL, 1.25 mg / mL, and 0.625 mg / mL, and the survival rate of zebrafish at 0.3125 mg / mL and 0.1562 mg / mL was 100%. Therefore, the maximum tolerance concentration of 2dpf zebrafish fry for the composition of Example 1 was 0.3125 mg / mL. In the subsequent Nile red staining experiments, Comparative Examples 1-3 and Example 1 were treated at the same concentration;

[0049] Table 2

[0050]

[0051] (3) Randomly select 2 dpf zebrafish fry with normal development, put them into a six-well culture plate, 15 per well, add 0.3125 mg / mL of the composition to each well, add 3 mL of fish culture water as a blank control group, and add 11.4 μg / mL resveratrol solution as a positive control group;

[0052] (4) 10 ng / mL Nile red solution was added to 3 dpf zebrafish for staining;

[0053] (5) At 4 dpf, 10 zebrafish were randomly selected from the blank control group, positive control group, and experimental group. The fluorescent markers were observed and photographed under a fluorescence microscope. The intensity of the fluorescence signal of zebrafish yolk sac fat was calculated using ImageJ software and statistically analyzed using GraphPad Prism. This indicator was used to evaluate the effect of each experimental group on promoting the decomposition of zebrafish fat.

[0054] like Figure 2 , 3 As shown, compared with the blank control group, the fluorescence intensity of zebrafish yolk sac fat treated with the positive control and Example 1 compositions was significantly weakened (p < 0.0001), indicating that the positive control and Example 1 compositions promoted the decomposition of zebrafish yolk sac fat after treatment, while the compositions of Comparative Examples 1, 2, and 3 did not significantly promote the decomposition of zebrafish fat (p > 0.05).

[0055] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A composition for improving basal metabolism during a low-carb diet, characterized in that: The composition comprises the following components in parts by weight: 0.01-0.5 parts of prickly pear cactus juice powder, 0.01-0.5 parts of guarana extract, 10-50 parts of virgin coconut oil microcapsule powder, 1-10 parts of medium chain triglyceride microcapsule powder, 0.1-1 parts of grape seed extract, 0.01-0.1 parts of potato extract and 0.1-1 parts of pomegranate extract.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 0.05 parts of prickly pear cactus juice powder, 0.05 parts of guarana extract, 30 parts of virgin coconut oil microcapsule powder, 3 parts of medium chain triglyceride microcapsule powder, 0.5 parts of grape seed extract, 0.05 parts of potato extract and 0.5 parts of pomegranate extract.

3. A method for preparing the composition according to claim 1 or 2, characterized in that: The method comprises the following steps: (1) preparing prickly pear cactus juice powder, guarana extract, virgin coconut oil microcapsule powder, medium chain triglyceride microcapsule powder, grape seed extract, potato extract, and pomegranate extract; (2) Mix all the components evenly and make the mixture into powder.

4. The method according to claim 3, characterized in that During the mixing process of step (2), a stirrer is used for mixing.

5. The method according to claim 4, characterized in that In step (2), the stirring speed of the stirrer is 500 rpm and the stirring time is 10 minutes.

6. The method according to claim 5, characterized in that The particle size of the powder in step (2) is 10 microns.

7. The method according to claim 6, characterized in that The method also includes step (3) packaging.

8. Use of the composition according to claim 1 or 2 in the preparation of a product for improving basal metabolism during a low-carb diet.

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