Application of Butylenephthalide

A technology of butylene phthalide and purpose, applied in the application field of butylene phthalide, can solve the problems of high cost, lack of technical means, infusion not meeting economic benefits and the like

Active Publication Date: 2021-06-22
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there is no effective technical means for the above two methods of increasing the proportion of brown fat in the body
Among them, although some studies have pointed out that exercise or a cold environment can stimulate fat browning in animals, modern people generally lack exercise and cold environments are not everywhere, and it is expected that exercise or a cold environment will be effective and stable. It becomes impractical to achieve the purpose of promoting fat browning in animals
In addition, due to the scarcity of sources of brown adipocytes / brown adipocytes, the cost of cell acquisition remains high, which also makes the infusion of brown adipocytes / brown adipocytes uneconomical

Method used

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  • Application of Butylenephthalide
  • Application of Butylenephthalide
  • Application of Butylenephthalide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1: The effect of butylenephthalide (BP) on promoting the differentiation of stem cells into brown adipocytes

[0064] As mentioned above, unlike white adipocytes, brown adipocytes / brown adipocytes have the characteristics of (i) containing many small fat droplets, (ii) containing a large number of mitochondria, and (iii) highly expressing UCP1 protein in cell morphology . Among them, due to containing a large number of mitochondria, compared with white fat, brown adipocytes / brown adipocytes have a larger oxygen consumption rate and potential respiratory capacity (wherein, potential respiratory capacity refers to the maximum oxygen consumption rate and the basic The difference in oxygen consumption rate represents the amount of oxygen not used to generate ATP). Therefore, by observing the number of small fat droplets in cells, the oxygen consumption rate of adipocytes, and the expression of UCP1 gene and protein, etc., we can understand the amount or ratio of br...

Embodiment 2

[0091] Embodiment 2: observe the situation of the fat browning of mice

[0092] It is known that brown adipocytes contain many small fat droplets. In order to know whether butylenephthalide (BP) has the effect of promoting fat browning in animals, subcutaneous adipose tissue and visceral adipose tissue provided by B2-3 were collected by Formalin-fixed, embedded in paraffin, and sectioned. The aforementioned sections were stained with hematoxylin-eosin stain (hematoxylin-sosin stain, H&E stain) to observe the cell morphology of the adipose tissue of mice in each group, and the results are shown in Figure 5A (subcutaneous fat) and Figure 5B (Visceral fat).

[0093] Depend on Figure 5A and Figure 5B It can be seen that the subcutaneous adipose tissue and visceral adipose tissue of the "HFD" group mice are composed of single and large white adipocytes, while the "HFD+BP" group, "HFD+ADSC" group, "HFD+BP+ADSC" group The subcutaneous adipose tissue and visceral adipose tiss...

Embodiment 3

[0095] Example 3: Appearance of heart, liver, kidney, subcutaneous adipose tissue and visceral adipose tissue of mice

[0096] In order to know whether butylenephthalide (BP) can improve the accumulation of white fat, the appearance photos of the liver, heart, kidney, subcutaneous fat tissue and visceral fat tissue of mice in each group recorded in B2-3 are shown in Figure 6A . The weights of subcutaneous adipose tissue and visceral adipose tissue of each group of mice recorded in B2-3 were averaged, and are shown in Table 1, and Figure 6B , Figure 6C .

[0097] Table 1

[0098] Subcutaneous fat tissue (grams) Visceral adipose tissue (grams) "ND" group 0.22 0.59 "HFD" group 0.42 1.0 "HFD+BP" group 0.24 0.57 "HFD+ADSC" group 0.31 0.62 "HFD+BP+ADSC" group 0.28 0.64 "HFD+BLC" group 0.21 0.54 "HFD+BP+BLC" group 0.20 0.46

[0099] Depend on Figure 6A It can be seen that compared with the "ND" group, the "HF...

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Abstract

The application of butylenephthalide (butylidenephthalide, BP) includes using butylenephthalide (BP) to provide a kind of kit for promoting the differentiation of stem cells into brown adipocytes, and using butylenephthalide (BP) in the manufacture of A use of a medicament, wherein the medicament is used for inhibiting the accumulation of white fat, promoting the transformation of white fat into brown fat, inhibiting weight gain and / or reducing the content of triglyceride, glucose and total cholesterol in blood.

Description

technical field [0001] The present invention relates to the application of butylenephthalide (butylidenephthalide, BP), especially about the application of using butylenephthalide (BP) to promote the differentiation of stem cells into brown adipocytes and the use of butylenephthalide (BP) in inhibiting white fat Accumulation, promoting the conversion of white fat to brown fat, inhibiting weight gain and / or lowering the levels of triglycerides, glucose and total cholesterol in the blood. Among them, by using butylenephthalide (BP) together with mesenchymal stem cells and / or brown adipocytes for an individual, it can provide more excellent inhibition of weight gain, reduction of adipose tissue accumulation, and promotion of white fat conversion into brown for the individual. Benefits of Fat. Background technique [0002] Fat can be roughly divided into two categories: white fat and brown fat. White fat is white in appearance and is composed of white fat cells, while brown fat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077A61K31/365A61P3/04A61P3/10A61P9/00A61P9/12A61P13/12
CPCA61K31/365C12N5/0653C12N2501/999C12N2501/33C12N2501/30C12N2500/40C12N2500/30A61K31/341A61P3/04A61K35/28A61K31/4439A61K31/661A61K31/522C12N2500/76A61K2300/00A61K35/35
Inventor 邱紫文林欣荣韩鸿志苏鸿麟刘诗平陆康芸谢济安
Owner DONGHUA UNIV
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