35 results about "Triptonide" patented technology

The invention discloses a method for preparing tripdiolide and 2-epitripdiolide. Structures of triptonide, triptolide, tripdiolide and 2-epitripdiolide in common threewingnut root are quite similar, wherein tripdiolide and 2-epitripdiolide are a pair of chiral isomers and difficult to separate. When the four compounds are separated in the prior art, the separation depends on repeated silica-gel column chromatography, however, the repeated silica-gel column chromatography is high in solvent consumption, low in repeatability, just suitable for a small amount of separation in a laboratory and difficult to popularize in industrial production. The column chromatography which is only in use in the current industrial production is macroporous resin. According to the method, triptonide, triptolide, tripdiolide and 2-epitripdiolide can be separated and prepared without dependence on the repeated silica-gel column chromatography. With adoption of the HPLC method, tripdiolide and 2-epitripdiolidein the chiral isomers can be effectively separated on the basis of a conventional C18 chromatographic column, the cost is low, and the repeatability is high.

The invention discloses a method for preparing triptonide. Structures of the triptonide, triptolide, tripdiolide and 2-epitripdiolide existed in common caulis tripterygii wilfordii are close, whereinthe tripdiolide and the 2-epitripdiolide are a pair of chiral isomers with big separating difficulty. The four compounds are separated by depending on repeated silica column chromatography in the prior art, while repeated silica column chromatography consumes solvent, is poor in reproducibility, only suitable for less separation in experiments, and hard to popularize in industrial production. In current industrial production, only used column chromatography is a macroporous resin. The method provided by the invention can be used for separating and preparing the triptonide, the triptolide, thetripdiolide and the 2-epitripdiolide without depending on repeated silica column chromatography. The HPLC (Hight Performance Liquid Chromatography) method provided by the invention can be used for effectively separating the chiral isomers i.e. the tripdiolide and the 2-epitripdiolide based on a conventional C18 chromatographic column, and is low in cost and high in repeatability.

The invention discloses a biogenic emulsifiable insecticide and a method for preparing the biogenic emulsifiable insecticide. The biogenic emulsifiable insecticide comprises the following components in percentage by weight: 5-15% of pyrethroid, 2-4% of triptonide, 8-15% of an emulsifier and the balance of an organic solvent. The triptonide and the pyrethroid are used together to take the synergistic effect to effectively improve the capacity of killing the insects such as the mosquitoes and the flies of the biogenic emulsifiable insecticide. Because the biogenic triptonide is added into the biogenic emulsifiable insecticide, one part of pyrethroid can be replaced effectively, and the biogenic emulsifiable insecticide is more environmental friendly. The biogenic emulsifiable insecticide can be diluted and sprayed to kill the insects such as the adult mosquitoes and the adult flies to reduce the insect density rapidly.

The invention discloses a modifying process of attenuation and synergy for Tripterygium wilfordii and its extract, wherein the procedure (1) comprises immersing Tripterygium wilfordii with acid solution, conversing Triptolidenol Triptonide into Tripchlo-rolide (II). While the procedure (2) comprises preparing concrete from Tripterygium wilfordii, then purifying Tripterygium wilfordii or its concrete, acid treating or immersing with acid solution.

The invention provides a method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations, which realizes detection by using a micro-emulsion eletrokinetic chromatography method. The method establishes the brand new micro-emulsion eletrokinetic chromatography method, and is used for detecting important toxicity and the content of effective constituents in thunder god vine in a separated manner. Experimental results show the method provided by the invention has the advantages of good linear relation and high precision; and the triptolide and the triptonide in a sample can reach the effect of the base line separation within 10 minutes, and the method is high in separation degree, can be used for detecting an actual sample, and provides powerful supplement for quality control of the thunder god vine medicinal materials or preparations.

Provided is a new use of polynucleotide-5' kinase-3' phosphatase. In particular, provided is the use of polynucleotide-5' kinase-3' phosphatase in the preparation of products for diagnosing pancreatic cancer. Also provided is the use of triptonide as a polynucleotide-5' kinase-3' phosphatase inhibitor. Further provided is the use of polynucleotide-5' kinase-3' phosphatase as a target in the preparation of a drug for treating pancreatic cancer.

The invention discloses a method for preparing triptolide. Triptonide, triptolide, tripdiolide and 2-epitripdiolide in common threewingnut roots are of very approximate structures, tripdiolide and 2-epitripdiolide are a pair of chiral isomers with large separation difficulties. In the prior art, the four compounds are separated all through repeated silica-gel column chromatography, however, repeated silica-gel column chromatography consumes a great amount of solvents and is poor in reproducibility, only applicable to slight separation in laboratories, and hard to popularize in industrial production. In conventional industrial production, only a macroporous resin is available as column chromatography in service. By adopting the method disclosed by the invention, triptonide, triptolide, tripdiolide and 2-epitripdiolide can be separated and prepared without repeated silica-gel column chromatography. On the basis of a common C18 chromatographic column, the HPLC (High Performance Liquid Chromatography) method disclosed by the invention is capable of effectively separating chiral isomers, namely tripdiolide and 2-epitripdiolide, and is low in cost and good in repeatability.