35 results about "Triptonide" patented technology

The invention provides a method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations, which realizes detection by using a micro-emulsion eletrokinetic chromatography method. The method establishes the brand new micro-emulsion eletrokinetic chromatography method, and is used for detecting important toxicity and the content of effective constituents in thunder god vine in a separated manner. Experimental results show the method provided by the invention has the advantages of good linear relation and high precision; and the triptolide and the triptonide in a sample can reach the effect of the base line separation within 10 minutes, and the method is high in separation degree, can be used for detecting an actual sample, and provides powerful supplement for quality control of the thunder god vine medicinal materials or preparations.

The invention discloses a method for preparing tripdiolide and 2-epitripdiolide. Structures of triptonide, triptolide, tripdiolide and 2-epitripdiolide in common threewingnut root are quite similar, wherein tripdiolide and 2-epitripdiolide are a pair of chiral isomers and difficult to separate. When the four compounds are separated in the prior art, the separation depends on repeated silica-gel column chromatography, however, the repeated silica-gel column chromatography is high in solvent consumption, low in repeatability, just suitable for a small amount of separation in a laboratory and difficult to popularize in industrial production. The column chromatography which is only in use in the current industrial production is macroporous resin. According to the method, triptonide, triptolide, tripdiolide and 2-epitripdiolide can be separated and prepared without dependence on the repeated silica-gel column chromatography. With adoption of the HPLC method, tripdiolide and 2-epitripdiolidein the chiral isomers can be effectively separated on the basis of a conventional C18 chromatographic column, the cost is low, and the repeatability is high.

The invention discloses a method for extracting and purifying 15-hydroxy triptonide. The 15-hydroxy triptonide of which the purity achieves 99 percent or higher can be extracted and obtained through the steps of taking root hearts, root barks, stems and leaves of triptolide as raw materials, crushing, soaking, percolating, extracting, silica gel column chromatography separation and purification, re-crystallizing and the like, and the yield of the 15-hydroxy triptonide is about 9*10<-5> and is improved by about 80 times in compassion with an existing literature report extraction process, so that a material basis is provided for the study on chemical structure, pharmacologic action and the like of the 15-hydroxy triptonide.

The invention discloses a biogenic source insect-proof fabric and a preparation method of the biogenic source insect-proof fabric. The preparation method of the biogenic source insect-proof fabric is characterized by comprising the following steps: (1) diluting biogenic source insect-proof missible oil for 50 to 100 times with water to form impregnation liquid; (2) impregnating the fabric into the impregnation liquid; drying by spinning and washing with the water; drying by spinning again and sizing to prepare the biogenic source insect-proof fabric provided by the invention. The biogenic source insect-proof missible oil provided by the invention is prepared from the following components in percentage by weight: 5 percent to 15 percent of pyrethroid, 2 percent to 4 percent of triptonide, 8 percent to 15 percent of an emulsifier and the balance of an organic solvent. The biogenic source insect-proof fabric prepared by the invention has a high invasion repelling capability on insects including mosquitoes and the like and has a lasting mosquito-repelling effect.

The invention discloses a method for preparing tripdiolide and 2-epitripdiolide. Structures of triptonide, triptolide, tripdiolide and 2-epitripdiolide in common threewingnut root are quite similar, wherein tripdiolide and 2-epitripdiolide are a pair of chiral isomers and difficult to separate. When the four compounds are separated in the prior art, the separation depends on repeated silica-gel column chromatography, however, the repeated silica-gel column chromatography is high in solvent consumption, low in repeatability, just suitable for a small amount of separation in a laboratory and difficult to popularize in industrial production. The column chromatography which is only in use in the current industrial production is macroporous resin. According to the method, triptonide, triptolide, tripdiolide and 2-epitripdiolide can be separated and prepared without dependence on the repeated silica-gel column chromatography. With adoption of the HPLC method, tripdiolide and 2-epitripdiolidein the chiral isomers can be effectively separated on the basis of a conventional C18 chromatographic column, the cost is low, and the repeatability is high.

The invention discloses a method for preparing triptonide. Structures of the triptonide, triptolide, tripdiolide and 2-epitripdiolide existed in common caulis tripterygii wilfordii are close, whereinthe tripdiolide and the 2-epitripdiolide are a pair of chiral isomers with big separating difficulty. The four compounds are separated by depending on repeated silica column chromatography in the prior art, while repeated silica column chromatography consumes solvent, is poor in reproducibility, only suitable for less separation in experiments, and hard to popularize in industrial production. In current industrial production, only used column chromatography is a macroporous resin. The method provided by the invention can be used for separating and preparing the triptonide, the triptolide, thetripdiolide and the 2-epitripdiolide without depending on repeated silica column chromatography. The HPLC (Hight Performance Liquid Chromatography) method provided by the invention can be used for effectively separating the chiral isomers i.e. the tripdiolide and the 2-epitripdiolide based on a conventional C18 chromatographic column, and is low in cost and high in repeatability.

The invention discloses a biogenic emulsifiable insecticide and a method for preparing the biogenic emulsifiable insecticide. The biogenic emulsifiable insecticide comprises the following components in percentage by weight: 5-15% of pyrethroid, 2-4% of triptonide, 8-15% of an emulsifier and the balance of an organic solvent. The triptonide and the pyrethroid are used together to take the synergistic effect to effectively improve the capacity of killing the insects such as the mosquitoes and the flies of the biogenic emulsifiable insecticide. Because the biogenic triptonide is added into the biogenic emulsifiable insecticide, one part of pyrethroid can be replaced effectively, and the biogenic emulsifiable insecticide is more environmental friendly. The biogenic emulsifiable insecticide can be diluted and sprayed to kill the insects such as the adult mosquitoes and the adult flies to reduce the insect density rapidly.

The invention discloses a modifying process of attenuation and synergy for Tripterygium wilfordii and its extract, wherein the procedure (1) comprises immersing Tripterygium wilfordii with acid solution, conversing Triptolidenol Triptonide into Tripchlo-rolide (II). While the procedure (2) comprises preparing concrete from Tripterygium wilfordii, then purifying Tripterygium wilfordii or its concrete, acid treating or immersing with acid solution.

The invention provides a method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations, which realizes detection by using a micro-emulsion eletrokinetic chromatography method. The method establishes the brand new micro-emulsion eletrokinetic chromatography method, and is used for detecting important toxicity and the content of effective constituents in thunder god vine in a separated manner. Experimental results show the method provided by the invention has the advantages of good linear relation and high precision; and the triptolide and the triptonide in a sample can reach the effect of the base line separation within 10 minutes, and the method is high in separation degree, can be used for detecting an actual sample, and provides powerful supplement for quality control of the thunder god vine medicinal materials or preparations.

Provided is a new use of polynucleotide-5' kinase-3' phosphatase. In particular, provided is the use of polynucleotide-5' kinase-3' phosphatase in the preparation of products for diagnosing pancreatic cancer. Also provided is the use of triptonide as a polynucleotide-5' kinase-3' phosphatase inhibitor. Further provided is the use of polynucleotide-5' kinase-3' phosphatase as a target in the preparation of a drug for treating pancreatic cancer.

The invention relates to a liquid chromatography-tandem mass spectrometry test method of wilforlide, triptonide, triptolide and tripterine. The test method includes the following steps: taking liquid samples or tissue samples, cutting into pieces and placing into a tube with a stopper; adding a certain amount of a sodium hydroxide solution into the samples to adjust the pH to be 9-10, adding ethyl acetate, oscillating for 10 minutes, and performing high speed centrifugation for 10 minutes; after separation of an organic phase, adding an organic solvent for secondary extraction, mixing the two obtained organic phases, and placing on a concentrator with the temperature of 50 DEG C for evaporation until the organic phases are dried; using an initial mobile phase to dilute the residue, enabling the obtained solution to pass through a 0.22-[um]m microporous organic membrane, and taking the filtrate for analysis by a liquid chromatography-tandem mass spectrometer. The test method provided by the invention is simple, efficient, quick, sensitive, high in accuracy and extensive in practicability, can be applied to qualitative and quantitative testing for wilforlide, triptonide, triptolide and tripterine in biological samples, and is suitable for tests on in-vitro samples and suspicious physical evidences.

The invention discloses a method for preparing triptolide. Triptonide, triptolide, tripdiolide and 2-epitripdiolide in common threewingnut roots are of very approximate structures, tripdiolide and 2-epitripdiolide are a pair of chiral isomers with large separation difficulties. In the prior art, the four compounds are separated all through repeated silica-gel column chromatography, however, repeated silica-gel column chromatography consumes a great amount of solvents and is poor in reproducibility, only applicable to slight separation in laboratories, and hard to popularize in industrial production. In conventional industrial production, only a macroporous resin is available as column chromatography in service. By adopting the method disclosed by the invention, triptonide, triptolide, tripdiolide and 2-epitripdiolide can be separated and prepared without repeated silica-gel column chromatography. On the basis of a common C18 chromatographic column, the HPLC (High Performance Liquid Chromatography) method disclosed by the invention is capable of effectively separating chiral isomers, namely tripdiolide and 2-epitripdiolide, and is low in cost and good in repeatability.