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Uses of triptolide for treating obesity and fatty liver

A kind of triptolide ketone, fat technology, applied in the field of triptolide ketone treatment of obesity and fatty liver

Pending Publication Date: 2019-11-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that it has male anti-fertility, anti-leukemia and anti-malignant tumor effects, but whether it has anti-obesity effects has not been reported at home and abroad.

Method used

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  • Uses of triptolide for treating obesity and fatty liver
  • Uses of triptolide for treating obesity and fatty liver
  • Uses of triptolide for treating obesity and fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 The weight loss effect of triptolide ketone treatment on obese mice

[0063] (1) Experimental materials

[0064] (1) Experimental animals:

[0065] Strain: ICR mice, male and female;

[0066] Source: Experimental Animal Center of Soochow University School of Medicine;

[0067] Certificate of Conformity: Experimental animal production license number: XCYK (Su) 2002-0008, experimental animal use license number: SYXK (Su) 2002-0037;

[0068] Weight: 18-22g;

[0069] Number of animals in each group: 8.

[0070] (2) Mouse feed formula: maltodextrin (12%), sucrose (18%), cholesterol (2%), imported lard (21%), casein (21%), whole milk powder (5%) , Alfalfa Powder (3%), Corn Starch (8.5%), L-Cystine (0.5%), Vegetable Oil (3%), Premix (6%).

[0071] (3) Test drug: Triptolide was diluted with normal saline to the desired concentration.

[0072] (2) Experimental method

[0073] 24 ICR mice, weighing 18-22g. They were randomly divided into three groups with 8 rat...

Embodiment 2

[0093] Example 2 triptolide inhibits fat accumulation in obese model mice

[0094] (1) Experimental materials

[0095] Mouse fat and liver tissues were from Example 1; the hematoxylin-eosin staining kit was purchased from Nanjing Jiancheng Biology Co., Ltd.

[0096] (2) Experimental method

[0097] The fixed tissue was dehydrated with graded ethanol, and the xylene was transparent for 1 hour, then the xylene in the tissue was replaced with paraffin to harden the tissue, and soaked in wax overnight at 55°C. Embed the wax-soaked tissue block in fresh paraffin to make a wax block. The wax dipping temperature is 66°C. After the paraffin is solidified, it is cooled and solidified immediately, and it is solidified at -20°C. After it is fully solidified, the wax block is taken out. The embedded tissue was fixed on a paraffin microtome for sectioning. Before dewaxing, place the tissue slices at room temperature in a 60°C incubator and bake for 30 minutes, soak the tissue slices in ...

Embodiment 3

[0100] Embodiment 3 oil red dyeing experiment

[0101] (1) Experimental materials

[0102] Mouse fat and liver tissues were obtained from Example 1; the oil red staining kit was purchased from Nanjing Jiancheng Biological Co., Ltd.

[0103] (2) Experimental method

[0104] Take out the frozen section, warm it up at room temperature, wash off the embedding agent with distilled water; soak in 60% isopropanol for 2 minutes, rinse in advance to facilitate oil red o staining; stain with oil red o working solution for 2-5 minutes, adjust with 60% isopropanol Coloring needs to be completed with the help of a microscope to adjust the appropriate color; after the coloring is completed, wash it with water immediately; counterstain with hematoxylin for 1 minute, the running water turns blue, and observe under the microscope; seal the slide with water-based mounting agent; take pictures with an upright microscope as soon as possible , Statistics of oil red o staining results.

[0105] ...

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Abstract

The present invention relates to the field of medicines, particularly relates to uses of triptolide for treating obesity and fatty liver, and specifically relates to applications of the triptolide inpreparation of drugs for treating and / or preventing the obesity and fatty liver. Experimental results show that after the treatment by the triptolide, the body weight, the fat accumulation, the liverfat production and liver coefficients of obese mice with high fat diet can be significantly reduced, and ALT, AST, LDL and CHO1 content in blood of the obese mice with the high fat diet are reduced, indicating that the triptolide has therapeutic effects on the obesity and fatty liver.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the use of triptolide for treating obesity and fatty liver. Background technique [0002] Obesity is an increasing epidemic disease all over the world and has become a global health problem. Whether in developing or developed countries, whether in adults or children, the incidence of obesity has increased dramatically. In recent years, more and more people suffer from fatty liver. Obesity and fatty liver have become potential risk factors for a variety of metabolic diseases, leading to increased morbidity and mortality of cardiovascular and cerebrovascular diseases and cancer. At present, the overweight rate of children in the world has reached 10%, and the obesity rate has reached 2%-3%. In the United States, the childhood obesity rate has increased by more than three times compared with the past 50 years. In China, the 2010 China Chronic Disease Inspection data shows that the overwei...

Claims

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Application Information

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IPC IPC(8): A61K31/585A61P3/04A61P1/16
CPCA61K31/585
Inventor 周泉生吕春萍曹志飞
Owner SUZHOU UNIV
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