29 results about "High lipids" patented technology

The invention discloses a lipid nano-micelle preparation method, which comprises: (1) providing an amphiphilic lipid alcoholic solution with a concentration of 20-50 mg / mL and a buffer solution, the buffer solution being selected from a PBS buffer solution, a citrate buffer solution, a Tris-HCl buffer solution or an acetate buffer solution; (2) mixing the alcoholic solution of the amphiphilic lipid and a buffer solution according to the flow velocity ratio of the alcoholic solution of the amphiphilic lipid to the buffer solution of (2-4): 1 by adopting microfluidic mixing equipment to obtain a mixed product; and (3) carrying out liquid change on the mixed product by using a buffer solution to remove the alcohol solvent. On the premise of ensuring the small particle size of the lipid nano-micelle, the uniformity and narrow particle size distribution of the lipid nano-micelle are improved, and the target of batch-to-batch reproducibility is achieved. The method does not need to use dangerous chemicals, is high in safety, is simple to operate, is low in amplification production difficulty by combining a microfluidic technology with a liquid change technology, is low in energy consumption, can effectively improve the production efficiency, and reduces the production cost.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

This invention discloses a polypeptide with anti-wrinkle and oil-controlling effects, belonging to the field of cosmetic polypeptide technology. The polypeptide is heptapeptide-6, with the amino acid sequence Asn-Asp-Glu-Gly-Leu-Tyr-Leu. At concentrations of 0.001 mg / mL to 0.1 mg / mL, heptapeptide-6 significantly promotes the synthesis of type I collagen and elastin by human skin fibroblasts, exhibiting excellent anti-wrinkle and firming activity. At concentrations of 1 ppm to 50 ppm, it significantly inhibits lipid accumulation in *C. elegans* and lipid expression in zebrafish, with a maximum lipid inhibition rate of 24.38%, and also demonstrates clear oil-controlling activity. This invention's polypeptide has a single structure, overcoming the shortcomings of existing skincare polypeptides with single efficacy and complex formulations. It features stable processing, high safety, and is suitable as a core active ingredient in anti-wrinkle and oil-controlling cosmetics, providing a new avenue for the development and application of multifunctional skincare polypeptides.

The invention relates to the technical field of biological information analysis, in particular to a goose follicular granule cell lipid droplet separation and multi-dimensional quality evaluation system and method, and the system comprises a lipid droplet image acquisition module, a lipid droplet separation module, a Raman spectrum detection module, a chemical component analysis module and a quality comprehensive evaluation module. According to the method, a multi-stage data acquisition system based on space identification and dynamic separation is constructed, so that the form of the lipid droplet at a single cell scale is accurately captured and positioned, and the target lipid droplet is screened and transferred by utilizing a fluid control strategy on the premise of not damaging the structure; high-purity molecular information is obtained by combining the field enhancement effect of spectral signals and lattice parameter tuning, the proportion difference of fatty acid and triglyceride is deconstructed through signal characteristics, the quantitative corresponding relation between morphological parameters and chemical compositions is established, multi-dimensional comprehensive characterization of lipid droplets is achieved, and the precision and result consistency of lipid metabolism state analysis are improved.

The present application provides a kind of wheat germ special composite enzyme inhibitor, wheat germ product and its preparation method, the wheat germ special composite enzyme inhibitor includes by mass percentage: rosemary acid 50-60%, bilberry extract 20-24%, green tea extract 14-17% and turmeric extract 5-9%.The present application is dissolved with hot water, homogenized sterilization after the composite enzyme inhibitor, then mixed with wheat germ, drying, so as to effectively inhibit 80%-90% of lipase activity, improve lipid stability, reduce the destruction of active ingredients, obtain long shelf life, less nutrient loss of wheat germ product.The present application process is simple, strong applicability, easy to large-scale industrial production.

The invention discloses a cationic lipid compound as well as a preparation method and application thereof, and the cationic lipid compound has the following structural characteristics: 1, the lipid compound has a multi-amino amidino head structure, so that the delivery efficiency of nucleic acid molecules can be improved; 2, the lipid compound has a phenylamidine structure, so that the structural stability of amidino can be improved, and the physical and chemical stability of lipid molecules can be improved; and 3, double biodegradation structures based on amidino and amidino bonds on two sides of a benzene ring in a lipid compound structure can be hydrolyzed and broken into small molecules after nucleic acid molecules are effectively delivered, so that the cell biocompatibility of lipid molecules can be improved, and the in-vivo safety can be improved. In conclusion, the multi-amino amidino cationic lipid molecule has the characteristics of high delivery efficiency, good cell biocompatibility and excellent storage stability, provides more choices for nucleic acid drug delivery, and has important significance for development and application of nucleic acid drugs.

This invention relates to the fields of environmental and bioanalytical technology, and particularly to a method for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. The technical solution includes a framework process for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. This invention establishes a dual-solvent secondary extraction system composed of n-hexane and methyl tert-butyl ether, achieving differentiated adaptation to different tissue matrices. By combining the advantages of non-polar and moderately polar solvents, it can simultaneously handle the relatively highly polar bisphenol S and the relatively non-polar bisphenol AF in a single extraction process. Furthermore, this method sets key physical parameters during the extraction process according to the histological characteristics of each organ. For heart and lung tissues, specific ultrasonic power is used to ensure thorough cell disruption; while for liver and spleen with high lipid content, different power and time are used to ensure the full release of lipid-bound target substances.

The invention relates to ceramide liposome as well as a preparation method and application thereof. The ceramide liposome is prepared from the following raw materials: water, an alcohol solvent, phospholipid, cholesterol, a co-emulsifier and an active component, the active components comprise ceramide and a candida albicans / glucose / flat nuclear wood oil fermentation product. The invention develops a liposome product simultaneously loading ceramide and candida albicans / glucose / flat nuclear wood oil fermentation products, the two active components are mutually matched and have synergistic interaction in the aspect of skin barrier repair, the transdermal permeation effect of the active components can be improved by virtue of a liposome form, and the bioavailability of the liposome product is improved. Compared with unfermented flat nuclear wood oil, the candida albicans / glucose / flat nuclear wood oil fermentation product is selected, so that the hydrophilicity and stability of the liposome can be improved, the encapsulation performance of the liposome on ceramide and the transdermal permeation effect of the ceramide can be improved, and finally the skin barrier repairing effect of the liposome product is improved.

The present invention relates to the use of a compound for the preparation of a composition for reversing higher lipid oxidation end products (ALE) and / or higher glycosylation end products (AGE). The compounds are represented by the following formula I, wherein R1, R2, R3 and R4 are as defined in the specification. The invention also relates to a composition for reversing ALE and / or AGE comprising said compound and to a cosmetic process comprising applying an effective amount of said compound to the skin of an organism.

The application provides a preparation method of a lipid nanoparticle and a complex thereof, wherein the lipid nanoparticle comprises an amino lipid compound, and the amino lipid compound comprises at least one of the following steps: compound (I), compound (II) and compound (III). The amino lipid compound has hydrophobic characteristics due to containing long non-polar residues, and has hydrophilic characteristics due to containing amino groups. Due to the amphiphilic characteristics, the amino lipid compound can be used to form the lipid nanoparticle, the hydrophobic part of the amino lipid compound helps to stabilize the structure of the lipid nanoparticle, and the amino group can produce strong hydrophilicity in the body, so that the water solubility and biocompatibility of the lipid nanoparticle are increased, the loading capacity and the encapsulation efficiency of the lipid nanoparticle are improved, the drug or gene delivery is more efficient, and the required administration dose is reduced and the side effects are reduced.

The invention discloses application of an HDAC (histone deacetylase) inhibitor to improvement of in-vivo delivery of lipid nanoparticles, and belongs to the technical field of gene therapy. HDACi is a small molecule compound for inhibiting histone deacetylase (HDAC), and the gene expression is influenced by inhibiting the HDAC so as to improve the acetylation level of histone or other non-histone substrates. After an organism is treated by the HDAC inhibitor, the in-vivo delivery efficiency of the lipid nanoparticles can be improved by 62%, and the effectiveness and safety of the nucleic acid medicine based on the lipid nanoparticles are improved. Therefore, the method disclosed by the invention lays a solid foundation for gene therapy based on lipid nanoparticles.

The present application describes the use of functionalized siloxane polymer for improving the intralipid resistance of aromatic polycarbonate, as is relevant especially for medical devices comprising elements which are intended to come into contact with intralipid solution during the intended use of the medical devices.

The application discloses a kind of DOC-Chaps synergistic core's clinical and animal tissue rapid transparent reagent and three-dimensional imaging method. Rapid transparent reagent is mainly dissolved in deionized water by sodium deoxycholate (DOC), 3-[(3-cholamide propyl) dimethylamino]-1-propane sulfonic acid (Chaps), urea and 1-methylimidazole; DOC efficiently dissolves high-density lipid, Chaps gently destroys membrane structure, both complement each other, realize lipid rapid, depth clearance and significantly reduce light scattering; urea loosens collagen fiber and protein aggregate, improves the penetration rate of reagent; 1-methylimidazole selectively removes hemoglobin, reduces light absorption. The transparent reagent of the application can make millimeter thick brain slices in 4 (mouse brain) / 6 (human brain) hours The light transmittance of specific wavelength is greater than or equal to 90%, and there is no obvious tissue shrinkage / expansion, the compatibility of fluorescent protein and antibody label is excellent, suitable for three-dimensional imaging of high-lipid, high-hemoglobin samples such as in-vitro human brain, kidney, liver and mouse brain, heart, kidney, liver, intestinal tract, embryo, etc.

The application discloses ionizable cationic lipids and application thereof. The ionizable cationic lipids of the application can form adsorption with biological drugs such as nucleic acids through structural design of a head part, so as to improve encapsulation efficiency; a saturated or unsaturated fatty chain is used to form a branched structure in a tail part, and an ester bond is used to connect the head part and the tail part, so as to facilitate in-vivo degradation and reduce toxicity; the design of the unsaturated fatty chain increases membrane fluidity during endosome release, so as to effectively improve the release efficiency of biological drugs; and the ionizable cationic lipids of the application have good temperature stability, which can not only improve product quality, but also expand product accessibility. In a word, the ionizable cationic lipids of the application have the advantages of high encapsulation efficiency, low toxicity, high endosome release efficiency and good temperature stability, can improve the quality and treatment effect of lipid nanoparticle drugs, and provide a new scheme and selection for biological drug delivery.

The present application relates to a strain of microalgae (Nannochloropsis gaditana) Micractinium sp. ) FSH-BY1, which has been preserved in the China General Microbiological Culture Collection Center on May 12, 2020, with a preservation number of CGMCC No. 19983. The present application also provides a culture method and application of the microalgae (Nannochloropsis gaditana) Micractinium sp. ) FSH-BY1. The microalgae provided by the present application can utilize carbon dioxide for rapid growth of light energy, obtain biomass rich in oil, and have the properties of tolerating SO2 and low temperature.

The application provides a liposome synthesis method and a synthesis device, and the method comprises the following steps: two liquids of an organic phase and an aqueous phase are respectively introduced into two sample introduction channels of a Y-shaped flow channel; the Y-shaped flow channel comprises the two sample introduction channels and a synthesis channel which is in communication with the converging position of the two sample introduction channels; a special ultrasonic device in the shape of a regular pentagon arranged at the bottom of the synthesis channel is driven to work, and at least through the micro-vortices generated in the liquid in the synthesis channel and distributed at the positions of the edges of the regular pentagon during the working of the special ultrasonic device, the two liquids in the synthesis channel are mixed to generate target liposomes. Through the cooperative design of the micro-flow channel structure and the special ultrasonic device, the synthesis efficiency of the liposomes can be significantly improved, and the average particle size and uniformity of the product in the synthesis process of the liposomes are optimized.

The invention belongs to the technical field of bioengineering, and discloses recombinant schizochytrium limacinum for producing DHA (docosahexaenoic acid) lipid by using carbon dioxide as well as a construction method and application of the recombinant schizochytrium limacinum, and the recombinant schizochytrium limacinum is obtained by introducing optimized encoding genes FDH, FK, FPR and Ti related to CO2 into schizochytrium limacinum; wherein the gene sequence of the gene FDH is SEQ ID No. 17, the gene sequence of the gene FK is SEQ ID No. 18, the gene sequence of the gene FPR is SEQ ID No. 19, and the gene sequence of the gene Ti is SEQ ID No. 20. The schizochytrium limacinum engineering bacterium capable of efficiently and rapidly utilizing the CO2 is constructed through a metabolic engineering means, and the schizochytrium limacinum engineering bacterium can utilize the CO2 as a carbon source to produce the DHA lipid, so that the production cost of the DHA lipid can be reduced, and the production economy of the DHA lipid can be improved.

Aspects of the present disclosure relate to compositions and methods for improving the stability of lipid nanoparticles (LNPs). In some embodiments, the LNPs contain one or more active pharmaceutical ingredients (APIs) encapsulated therein. The present disclosure is based, in part, on compositions that directly or indirectly reduce degradation (e.g., oxidation, hydrolysis, etc.) of one or more lipid components of the lipid nanoparticles. In some embodiments, the compositions contain a histidine buffer. The present disclosure also provides methods for storing the compositions contemplated herein, as well as methods for improving the stability of APIs.

The invention discloses a method for detecting mitochondrial membrane potential of gill or hepatopancreas of crustaceans, and belongs to the technical field of mitochondrial membrane potential detection. Differentiated incubation strategies are respectively formulated according to the structure and physiological difference of gill and hepatopancreas tissues of crustaceans: a method of'in-vivo pre-balancing-short-time staining-rapid flaking 'is adopted for the gill tissues, JC-1 incubation time is strictly controlled within 10 min, and damage of probe toxicity to mitochondria is effectively avoided; hepatopancreas tissues are incubated for 1h by adopting a prolonged living body, so that the sufficient permeation of the dye is ensured. According to the method, under the condition that 2 [mu] M JC-1 and 20 [mu] mol / L CCCP are uniformly used as positive control, the tissue permeability and signal uniformity of the dye are remarkably improved, the problems of uneven dyeing, weak signals and the like caused by exoskeleton barrier and high lipid content are solved, and reliable technical support is provided for aquatic product health assessment and toxicological research.

The application discloses an organic small-molecule fluorescent probe and a preparation method thereof, and belongs to the technical field of biological fluorescent labeling. 16 The application discloses a D-pi-A type near-infrared fluorescent probe with straight-chain alkyl aniline as an electron donor, which discloses for the first time that the lipid droplet targeting imaging performance of the probe is negatively correlated with the weakening of the length of the alkyl chain, and the C4 probe with the optimal comprehensive performance is screened. The probe has the advantages of near-infrared emission, large stokes shift, high lipid droplet specificity and low cytotoxicity, and the preparation method is universal, mild and easy to scale up, and can be widely applied to the research and diagnosis fields of live cell lipid droplet dynamic imaging and lipid metabolism related diseases.

The application provides a preparation method of liposomes. The liposomes are prepared by using lecithin containing calcium citrate as a liposome film material and using a water-soluble solution containing a water-soluble active ingredient as a hydration solution. By mixing calcium citrate in the liposome film, the active ingredient in the liposomes can be quickly released, and the stability of the liposomes can be significantly improved. The raw materials used are non-toxic to human bodies, have high safety, and are suitable for preparing products such as medicines, oral care products and oral health care products.

The present disclosure relates to a nano-delivery carrier and a preparation method therefor. Specifically, the present disclosure relates to an improved nanoscale lipid carrier for nucleic acid delivery or a delivery system thereof. The carrier comprises an ionizable lipid, a helper lipid, a PEGylated lipid, cholesterol, and a cationic lipid. The nano-delivery carrier of the present disclosure solves the problems of low encapsulation efficiency, batch-to-batch inconsistency of artificially mixed particles, high cationic lipid ratio, high lipid-to-drug ratio, and weak immunogenicity in the prior art.

The invention discloses an intelligent hydrogel dressing and a preparation method thereof, and belongs to the field of biomedical materials and intelligent controlled release systems. The dressing comprises a liposome microcapsule system and a piezoelectric ionic gel matrix, and gel is composed of dimethylaminoethyl methacrylate, hydroxyethyl methylacrylate, sulfonated sodium alginate and the like and can generate an electric signal under mechanical stimulation; the liposome microcapsule system entraps a drug for wound treatment, when an electric signal output by the piezoelectric gel acts on the liposome, the membrane permeability of the liposome can be triggered to be increased, and on-demand controlled release is realized. Furthermore, a three-stage embedding structure is constructed through arginine, so that the stability of the liposome in the gel can be remarkably improved. The dressing disclosed by the invention can realize the intelligent controlled release functions of self power supply, dynamic healing promotion, antibiosis and motion coupling, and is suitable for treating chronic wound difficult to heal.

This invention provides the application of transcription factor CUX1 in the preparation of products for the prevention and treatment of diabetic foot ulcers, belonging to the field of biomedical technology. Its key technical point lies in providing the application of transcription factor CUX1 as a target in the preparation of products for the prevention and treatment of diabetic foot ulcers. This application confirms the high expression of transcription factor CUX1 in diabetic foot ulcer wound tissue through specific verification experiments. Using a diabetic rat model of ischemic foot ulcers in the lower limbs, this invention is the first to clearly demonstrate a significant increase in the expression of transcription factor CUX1 in diabetic ulcer wound tissue. Using a high-glucose, high-lipid cell injury model, it is the first to demonstrate that CUX1 expression is significantly high after fibroblast injury due to high glucose and high lipid levels. It is the first to discover that transcription factors have a protective effect on fibroblasts injured by high glucose and high lipid levels. This invention provides a new approach for the treatment of diabetic foot ulcers and a new target for the development of diabetic wound healing.

The application discloses an acid oligopeptide modified bone targeting liposome and a preparation method thereof. The surface of the bone targeting liposome is modified with acid oligopeptide, and the modification density n is 1-5, which is expressed in mol%. The bone targeting liposome is prepared by modifying the liposome surface with D-glutamic acid hexapeptide which has bone targeting effect, and optimizing the modification density of the D-glutamic acid hexapeptide on the liposome surface, so that the liposome preparation with the strongest bone targeting activity is screened, and the bone tissue distribution of the liposome preparation is increased. With the increase of the modification density of the acid oligopeptide, the combination ability of the liposome and hydroxyapatite is enhanced. However, the high negative charge generated by the modification stimulates the phagocytosis of the mononuclear phagocyte system, and the blood retention of the liposome is reduced. However, the high-density acid oligopeptide modification (5 mol%) endows the liposome with outstanding HA combination activity, and the deficiency of the weak blood retention of the liposome is completely compensated, so that the highest bone targeting delivery is realized.

The invention belongs to the technical field of nano materials, and particularly relates to F4 / 80 antibody coupled lipid nanoparticles as well as a preparation method and application thereof. The F4 / 80 antibody coupled lipid nanoparticle comprises a lipid nanoparticle and a sulfydryl modified F4 / 80 antibody, wherein the sulfydryl modified F4 / 80 antibody is a sulfydryl modified F4 / 80 antibody; the F4 / 80 antibody modified by the sulfydryl is coupled to the lipid nanoparticles, and the F4 / 80 antibody modified by the sulfydryl is coupled to the lipid nanoparticles; the molar ratio of the lipid nanoparticles to the sulfydryl modified F4 / 80 antibody is (3-5): 1; the sulfydryl-modified F4 / 80 antibody is a modified antibody obtained by introducing sulfydryl into an F4 / 80 antibody; the amino acid sequence of the F4 / 80 antibody is as shown in SEQ ID NO. 1; the lipid nanoparticles are nanoparticles which take monophosphoryl lipid A as an adjuvant and are loaded with IFN (interferon) gamma mRNA (messenger Ribonucleic Acid) at the same time. According to the F4 / 80 antibody coupled lipid nanoparticle provided by the invention, the in-vivo and in-vitro targeting and transfection efficiency of the lipid nanoparticle can be improved.

This invention discloses a method for fermenting and culturing *Yarrowia lipolytica* for feed using waste liquor from corn processing for sugar refining. The invention uses glucose crystallization secondary mother liquor and corn steep liquor, the main waste products from corn processing for sugar refining, as the carbon and nitrogen sources, respectively. *Yarrowia lipolytica* is cultured in a fermenter using an aerobic fed-batch method. The required culture medium and feed solution, apart from necessary pH adjustment and the addition of an antifoaming agent, only require the secondary mother liquor and corn steep liquor. With the culture medium and fermentation operations remaining the same in the early stages of fermentation, the only change in the later feeding stage is the ratio of the secondary mother liquor and corn steep liquor in the feed solution. This achieves the goals of high lipid accumulation, high protein accumulation, and increased cell yield in the cultured *Yarrowia lipolytica* cells, without altering the characteristics of the accumulated lipids being high in polyunsaturated fatty acids and the proteins being rich in essential amino acids such as lysine, serine, and leucine.

The invention provides a preparation method of liposome. According to the invention, lecithin containing calcium citrate is used as a liposome film material, and an aqueous solution containing a water-soluble active component is used as a hydration solution to prepare the liposome. The calcium citrate is mixed in the liposome film, active ingredients in the liposome can be rapidly released, the stability of the liposome can be remarkably improved, the used raw materials are non-toxic to human bodies, high safety is achieved, and the liposome is suitable for preparing products such as drugs, oral care products and oral health care products.