52 results about "High lipids" patented technology

The invention discloses a lipophilic liposome containing polydeoxyribonucleotide as well as a preparation method and application of the lipophilic liposome. The lipophilic liposome comprises the following components in parts by weight: 0.001-3 parts of polydeoxyribonucleotide; 0.5 to 3 parts of phospholipid; 0.1-2 parts of a cationic emulsifier; 0.1-3 parts of an anionic emulsifier; 40 to 70 parts of polyol; the total amount is 100 parts. According to the invention, an emulsifier system formed by combining lecithin with anionic and cationic compound surfactants is adopted, so that the transdermal absorption of PDRN is facilitated. The lipophilic liposome containing polydeoxyribonucleotide prepared by the invention can effectively resist nuclease degradation, prolong the acting time of PDRN in skin, and improve the skin anti-aging and repairing effects of the liposome. The anti-aging effect of the active ingredients is improved, and the bioavailability of the active ingredients is improved. The cosmetic composition can be widely applied to cosmetic formulas of various dosage forms.

The invention relates to double-encapsulated lipid nanoparticles entering brain through nose and a preparation method and application thereof, the double-encapsulated lipid nanoparticles comprise lipid nanoparticles, drugs in the lipid nanoparticles and rana odorata lectin coupled to the surfaces of the lipid nanoparticles, and the drugs comprise hydrophilic drugs and hydrophobic drugs. According to the invention, the rana odorata lectin and the lipid nanoparticles are coupled, and the rana odorata lectin can be specifically combined with L-fucose residues expressed by olfactory epithelial cells, so that the adhesion of the lipid nanoparticles to olfactory epithelium during nasal administration is enhanced, and the brain entering efficiency of the lipid nanoparticles is finally improved. After entering the brain, the lipid nanoparticles respond to diseased regions with high active oxygen content so as to release drugs. In addition, double-drug encapsulation can target different pathogenesis of central nervous system diseases, and the limitation that a traditional treatment drug is single in action target is improved.

The invention discloses a cationic lipid capable of being ionized and application of the cationic lipid. According to the ionizable cationic grease, adsorption is formed by the structural design of the head and biological drugs such as nucleic acid, and the encapsulation efficiency is improved; the tail part adopts a saturated or unsaturated aliphatic chain to form a branch structure, and the head part and the tail part are connected by an ester bond, so that in-vivo degradation is facilitated to reduce toxicity; due to the design of the unsaturated aliphatic chain, the membrane flowability during release of the endosome is improved, and the release efficiency of the biological medicine is effectively improved; moreover, the ionizable cationic grease provided by the invention has good temperature stability, not only can the product quality be improved, but also the accessibility of the product is expanded. In a word, the ionizable cationic lipid disclosed by the invention has the advantages of high encapsulation efficiency, low toxicity, high endosome release efficiency, good temperature stability and the like, the quality and the treatment effect of lipid nanoparticle drugs can be improved, and a new scheme and a new choice are provided for biological drug delivery.

The invention discloses liposome nanoparticles as well as a preparation method and application thereof, and relates to the field of biological medicines. The liposome nanoparticle comprises a lipid compound modified with a fusion protein, the fusion protein comprises apolipoprotein E or polypeptide thereof and an antibody, the modification of the fusion protein not only can actively target cells, tissues or organs of corresponding ligands, but also can effectively improve the transfection efficiency and the targeting delivery capability of the liposome nanoparticle to the cells, and the targeting delivery capability of the liposome nanoparticle to the cells can be effectively improved. The nucleic acid delivery system can be applied to preparation of anti-tumor drugs.

The present invention discloses a method for improving the liposome encapsulation efficiency by modifying a cholesterol composite prodrug. Specifically, the drug curcumin and vitamin E succinate are first reacted with cholesterol to obtain a cholesterol-curcumin complex and a cholesterol-vitamin E succinate complex. The two obtained complexes are then uniformly mixed with a lipid and then prepared by thin film hydration to obtain drug-loaded liposomes. The present invention utilizes the cholesterol composite prodrug method to improve the low solubility of curcumin and vitamin E succinate in liposomes, improve the encapsulation efficiency of the two drugs in liposomes, and provide a new approach for the preparation of liposomes containing poorly soluble compounds.

The invention discloses a lipid nano-micelle preparation method, which comprises: (1) providing an amphiphilic lipid alcoholic solution with a concentration of 20-50 mg / mL and a buffer solution, the buffer solution being selected from a PBS buffer solution, a citrate buffer solution, a Tris-HCl buffer solution or an acetate buffer solution; (2) mixing the alcoholic solution of the amphiphilic lipid and a buffer solution according to the flow velocity ratio of the alcoholic solution of the amphiphilic lipid to the buffer solution of (2-4): 1 by adopting microfluidic mixing equipment to obtain a mixed product; and (3) carrying out liquid change on the mixed product by using a buffer solution to remove the alcohol solvent. On the premise of ensuring the small particle size of the lipid nano-micelle, the uniformity and narrow particle size distribution of the lipid nano-micelle are improved, and the target of batch-to-batch reproducibility is achieved. The method does not need to use dangerous chemicals, is high in safety, is simple to operate, is low in amplification production difficulty by combining a microfluidic technology with a liquid change technology, is low in energy consumption, can effectively improve the production efficiency, and reduces the production cost.

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and / or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

The invention provides a preparation method of liver-targeted drug-loaded liposome, and belongs to the field of biological medicine. The hepatic targeting carbohydrate chain is constructed through a multi-step enzymatic reaction, and the accuracy of a modification site is ensured. The synthesized galactose ligand is connected to the surface of the liposome through a covalent bond, so that the targeting property and the stability are enhanced. The synthesis of the galactose ligand provides targeting functional groups with clear structures for the liposome, and the groups are integrated to the surface of the liposome through physical entrapment and chemical coupling in the preparation of the liposome, so that active targeting delivery is finally realized. The collaborative design of the two is based on an ASGPR-mediated liver targeting mechanism, and the delivery efficiency is optimized through enzymatic synthesis and a nanotechnology. In in vivo experiments, plasma and liver distributions of liposomes are determined. In an in-vitro experiment, the uptake efficiency of the lipidosome in liver cancer cells is determined. The experiments aim at verifying that the advantages of the lipidosome in liver cancer cell delivery are improved by optimizing the PEG chain length and the exposure of residues.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

This invention discloses a polypeptide with anti-wrinkle and oil-controlling effects, belonging to the field of cosmetic polypeptide technology. The polypeptide is heptapeptide-6, with the amino acid sequence Asn-Asp-Glu-Gly-Leu-Tyr-Leu. At concentrations of 0.001 mg / mL to 0.1 mg / mL, heptapeptide-6 significantly promotes the synthesis of type I collagen and elastin by human skin fibroblasts, exhibiting excellent anti-wrinkle and firming activity. At concentrations of 1 ppm to 50 ppm, it significantly inhibits lipid accumulation in *C. elegans* and lipid expression in zebrafish, with a maximum lipid inhibition rate of 24.38%, and also demonstrates clear oil-controlling activity. This invention's polypeptide has a single structure, overcoming the shortcomings of existing skincare polypeptides with single efficacy and complex formulations. It features stable processing, high safety, and is suitable as a core active ingredient in anti-wrinkle and oil-controlling cosmetics, providing a new avenue for the development and application of multifunctional skincare polypeptides.

The invention relates to the technical field of biological information analysis, in particular to a goose follicular granule cell lipid droplet separation and multi-dimensional quality evaluation system and method, and the system comprises a lipid droplet image acquisition module, a lipid droplet separation module, a Raman spectrum detection module, a chemical component analysis module and a quality comprehensive evaluation module. According to the method, a multi-stage data acquisition system based on space identification and dynamic separation is constructed, so that the form of the lipid droplet at a single cell scale is accurately captured and positioned, and the target lipid droplet is screened and transferred by utilizing a fluid control strategy on the premise of not damaging the structure; high-purity molecular information is obtained by combining the field enhancement effect of spectral signals and lattice parameter tuning, the proportion difference of fatty acid and triglyceride is deconstructed through signal characteristics, the quantitative corresponding relation between morphological parameters and chemical compositions is established, multi-dimensional comprehensive characterization of lipid droplets is achieved, and the precision and result consistency of lipid metabolism state analysis are improved.

The invention relates to the technical field of feed and feed additives. The invention provides an astaxanthin-rich cladophora sp. SYSU-A2024-1, the classification name of the cladophora sp. SYSU-A2024-1 is Oedacladium sp.SYSU-A2024-1, the cladophora sp. SYSU-A2024-1 is preserved in China Center for Type Culture Collection on June 3, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241138. The invention also provides a composition containing the cladophora sp. SYSU-A2024-1 and the chrysophyta laminarin, and an application of the composition in preparation of a low-fish-meal feed for aquatic animals. The trachinotus ovatus low-fish-meal feed containing the composition prepared by the invention can more comprehensively improve the lipid utilization rate of trachinotus ovatus, relieve oxidative stress and inflammatory injury, improve the liver health of fish bodies and remarkably improve the growth performance of the fish bodies through synergistic interaction, thereby ensuring the health and vitality of the fish bodies.

The present application provides a kind of wheat germ special composite enzyme inhibitor, wheat germ product and its preparation method, the wheat germ special composite enzyme inhibitor includes by mass percentage: rosemary acid 50-60%, bilberry extract 20-24%, green tea extract 14-17% and turmeric extract 5-9%.The present application is dissolved with hot water, homogenized sterilization after the composite enzyme inhibitor, then mixed with wheat germ, drying, so as to effectively inhibit 80%-90% of lipase activity, improve lipid stability, reduce the destruction of active ingredients, obtain long shelf life, less nutrient loss of wheat germ product.The present application process is simple, strong applicability, easy to large-scale industrial production.

The invention provides a synthesis method and a synthesis device of lipidosome, and the method comprises the following steps: respectively introducing two liquids of an organic phase and a water phase into two sample introduction channels of a Y-shaped flow channel; the Y-shaped flow channel comprises the two sample introduction channels and a synthesis channel communicated with the convergence position of the two sample introduction channels; and driving a regular pentagonal special ultrasonic device arranged at the bottom of the synthesis channel to work, and mixing the two liquids in the synthesis channel at least through micro vortexes which are generated in the liquids in the synthesis channel and are distributed at the positions of the edges of the regular pentagon when the special ultrasonic device works, so as to generate the target liposome. Through collaborative design of the micro-channel structure and the special ultrasonic device, the synthesis efficiency of the lipidosome can be remarkably improved, and the average particle size and uniformity of a product in the synthesis process of the lipidosome are optimized.

The invention discloses a cationic lipid compound as well as a preparation method and application thereof, and the cationic lipid compound has the following structural characteristics: 1, the lipid compound has a multi-amino amidino head structure, so that the delivery efficiency of nucleic acid molecules can be improved; 2, the lipid compound has a phenylamidine structure, so that the structural stability of amidino can be improved, and the physical and chemical stability of lipid molecules can be improved; and 3, double biodegradation structures based on amidino and amidino bonds on two sides of a benzene ring in a lipid compound structure can be hydrolyzed and broken into small molecules after nucleic acid molecules are effectively delivered, so that the cell biocompatibility of lipid molecules can be improved, and the in-vivo safety can be improved. In conclusion, the multi-amino amidino cationic lipid molecule has the characteristics of high delivery efficiency, good cell biocompatibility and excellent storage stability, provides more choices for nucleic acid drug delivery, and has important significance for development and application of nucleic acid drugs.

This invention relates to the fields of environmental and bioanalytical technology, and particularly to a method for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. The technical solution includes a framework process for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. This invention establishes a dual-solvent secondary extraction system composed of n-hexane and methyl tert-butyl ether, achieving differentiated adaptation to different tissue matrices. By combining the advantages of non-polar and moderately polar solvents, it can simultaneously handle the relatively highly polar bisphenol S and the relatively non-polar bisphenol AF in a single extraction process. Furthermore, this method sets key physical parameters during the extraction process according to the histological characteristics of each organ. For heart and lung tissues, specific ultrasonic power is used to ensure thorough cell disruption; while for liver and spleen with high lipid content, different power and time are used to ensure the full release of lipid-bound target substances.

The invention relates to ceramide liposome as well as a preparation method and application thereof. The ceramide liposome is prepared from the following raw materials: water, an alcohol solvent, phospholipid, cholesterol, a co-emulsifier and an active component, the active components comprise ceramide and a candida albicans / glucose / flat nuclear wood oil fermentation product. The invention develops a liposome product simultaneously loading ceramide and candida albicans / glucose / flat nuclear wood oil fermentation products, the two active components are mutually matched and have synergistic interaction in the aspect of skin barrier repair, the transdermal permeation effect of the active components can be improved by virtue of a liposome form, and the bioavailability of the liposome product is improved. Compared with unfermented flat nuclear wood oil, the candida albicans / glucose / flat nuclear wood oil fermentation product is selected, so that the hydrophilicity and stability of the liposome can be improved, the encapsulation performance of the liposome on ceramide and the transdermal permeation effect of the ceramide can be improved, and finally the skin barrier repairing effect of the liposome product is improved.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive containing chitosan oligosaccharide and mulberry leaf flavone and application of the feed additive. The invention discloses a feed additive containing chitosan oligosaccharide and mulberry leaf flavone. The feed additive can be applied to promoting digestion of beef cattle and maintaining rumen health after being added into a basal feed. Chitosan oligosaccharide and folium mori flavone influence microbial communities through mediation on changes of metabolites such as N-(3-oxyhexanoyl) homoserine lactone, morusin, mulberrin and auraflavones, the effect of improving lipid metabolism and the effect of resisting oxidation are achieved, differential microorganisms play respective roles, and the effect of improving lipid metabolism is achieved. The influence is generated on the nutrient substance apparent digestibility and rumen fermentation performance of the beef cattle. The animal test also verifies the result of the in-vitro test, and determines that the chitosan oligosaccharide and the mulberry leaf flavone have interaction, the chitosan oligosaccharide and the mulberry leaf flavone have good effects on improving nutrient substance digestion and maintaining rumen health, and the test animal does not have adverse conditions.

The present invention relates to the use of a compound for the preparation of a composition for reversing higher lipid oxidation end products (ALE) and / or higher glycosylation end products (AGE). The compounds are represented by the following formula I, wherein R1, R2, R3 and R4 are as defined in the specification. The invention also relates to a composition for reversing ALE and / or AGE comprising said compound and to a cosmetic process comprising applying an effective amount of said compound to the skin of an organism.

The application provides a preparation method of a lipid nanoparticle and a complex thereof, wherein the lipid nanoparticle comprises an amino lipid compound, and the amino lipid compound comprises at least one of the following steps: compound (I), compound (II) and compound (III). The amino lipid compound has hydrophobic characteristics due to containing long non-polar residues, and has hydrophilic characteristics due to containing amino groups. Due to the amphiphilic characteristics, the amino lipid compound can be used to form the lipid nanoparticle, the hydrophobic part of the amino lipid compound helps to stabilize the structure of the lipid nanoparticle, and the amino group can produce strong hydrophilicity in the body, so that the water solubility and biocompatibility of the lipid nanoparticle are increased, the loading capacity and the encapsulation efficiency of the lipid nanoparticle are improved, the drug or gene delivery is more efficient, and the required administration dose is reduced and the side effects are reduced.

The invention discloses application of an HDAC (histone deacetylase) inhibitor to improvement of in-vivo delivery of lipid nanoparticles, and belongs to the technical field of gene therapy. HDACi is a small molecule compound for inhibiting histone deacetylase (HDAC), and the gene expression is influenced by inhibiting the HDAC so as to improve the acetylation level of histone or other non-histone substrates. After an organism is treated by the HDAC inhibitor, the in-vivo delivery efficiency of the lipid nanoparticles can be improved by 62%, and the effectiveness and safety of the nucleic acid medicine based on the lipid nanoparticles are improved. Therefore, the method disclosed by the invention lays a solid foundation for gene therapy based on lipid nanoparticles.

The present application describes the use of functionalized siloxane polymer for improving the intralipid resistance of aromatic polycarbonate, as is relevant especially for medical devices comprising elements which are intended to come into contact with intralipid solution during the intended use of the medical devices.

The application discloses a kind of DOC-Chaps synergistic core's clinical and animal tissue rapid transparent reagent and three-dimensional imaging method. Rapid transparent reagent is mainly dissolved in deionized water by sodium deoxycholate (DOC), 3-[(3-cholamide propyl) dimethylamino]-1-propane sulfonic acid (Chaps), urea and 1-methylimidazole; DOC efficiently dissolves high-density lipid, Chaps gently destroys membrane structure, both complement each other, realize lipid rapid, depth clearance and significantly reduce light scattering; urea loosens collagen fiber and protein aggregate, improves the penetration rate of reagent; 1-methylimidazole selectively removes hemoglobin, reduces light absorption. The transparent reagent of the application can make millimeter thick brain slices in 4 (mouse brain) / 6 (human brain) hours The light transmittance of specific wavelength is greater than or equal to 90%, and there is no obvious tissue shrinkage / expansion, the compatibility of fluorescent protein and antibody label is excellent, suitable for three-dimensional imaging of high-lipid, high-hemoglobin samples such as in-vitro human brain, kidney, liver and mouse brain, heart, kidney, liver, intestinal tract, embryo, etc.

The application discloses ionizable cationic lipids and application thereof. The ionizable cationic lipids of the application can form adsorption with biological drugs such as nucleic acids through structural design of a head part, so as to improve encapsulation efficiency; a saturated or unsaturated fatty chain is used to form a branched structure in a tail part, and an ester bond is used to connect the head part and the tail part, so as to facilitate in-vivo degradation and reduce toxicity; the design of the unsaturated fatty chain increases membrane fluidity during endosome release, so as to effectively improve the release efficiency of biological drugs; and the ionizable cationic lipids of the application have good temperature stability, which can not only improve product quality, but also expand product accessibility. In a word, the ionizable cationic lipids of the application have the advantages of high encapsulation efficiency, low toxicity, high endosome release efficiency and good temperature stability, can improve the quality and treatment effect of lipid nanoparticle drugs, and provide a new scheme and selection for biological drug delivery.

The invention relates to the technical field of biology, in particular to schizochytrium limacinum LH-G2 for co-production of DHA (docosahexaenoic acid) and carotenoid. The invention provides schizochytrium limacinum LH-G2 with the preservation number of CCTCC NO: M 2025675, the schizochytrium limacinum LH-G2 is obtained through adaptive evolution, and the schizochytrium limacinum LH-G2 is high in content of lipids such as DHA and carotenoid, can be used for industrial production of lipids such as DHA and carotenoid, and has important popularization and application values.

The present application relates to a strain of microalgae (Nannochloropsis gaditana) Micractinium sp. ) FSH-BY1, which has been preserved in the China General Microbiological Culture Collection Center on May 12, 2020, with a preservation number of CGMCC No. 19983. The present application also provides a culture method and application of the microalgae (Nannochloropsis gaditana) Micractinium sp. ) FSH-BY1. The microalgae provided by the present application can utilize carbon dioxide for rapid growth of light energy, obtain biomass rich in oil, and have the properties of tolerating SO2 and low temperature.

The application provides a liposome synthesis method and a synthesis device, and the method comprises the following steps: two liquids of an organic phase and an aqueous phase are respectively introduced into two sample introduction channels of a Y-shaped flow channel; the Y-shaped flow channel comprises the two sample introduction channels and a synthesis channel which is in communication with the converging position of the two sample introduction channels; a special ultrasonic device in the shape of a regular pentagon arranged at the bottom of the synthesis channel is driven to work, and at least through the micro-vortices generated in the liquid in the synthesis channel and distributed at the positions of the edges of the regular pentagon during the working of the special ultrasonic device, the two liquids in the synthesis channel are mixed to generate target liposomes. Through the cooperative design of the micro-flow channel structure and the special ultrasonic device, the synthesis efficiency of the liposomes can be significantly improved, and the average particle size and uniformity of the product in the synthesis process of the liposomes are optimized.

The invention discloses lactobacillus plantarum LP-G7 and application thereof, and the lactobacillus plantarum LP-G7 provided by the invention is acid-resistant and cholate-resistant, has acid production, bacteriostasis and adhesion capabilities, and can improve the growth performance of meat ducks, reduce the deposition of fat in serum, abdomen and liver, improve lipid metabolism and immunocompetence and improve the meat quality of the meat ducks. The method can be widely applied to meat duck breeding, and favorable support is provided for high-quality development of meat duck breeding.

The invention provides Porro lactobacillus mucus with a lipid-lowering function and application of the Porro lactobacillus mucus, and belongs to the technical field of microorganisms. The invention relates to a lactobacillus mucus strain FVT08 of Porro, and the preservation number of the lactobacillus mucus strain FVT08 is CGMCC (China General Microbiological Culture Collection Center) No.33640. The strain FVT08 not only has high lipid-lowering capacity under in-vitro conditions, can inhibit the activity of lipase (pancreatic lipase and cholesterol esterase) to reduce fat absorption of a body, but also has high bile salt hydrolase activity to promote cholesterol discharge, and at the animal level, the strain FVT08 colonizes in intestinal tracts to promote cholesterol discharge. The liver blood fat level and the liver tissue antioxidant level are reduced, lipid metabolism related gene expression and intestinal flora disorder are improved, and the content of short-chain fatty acids (propionic acid, n-butyric acid, isobutyric acid, n-valeric acid and isovaleric acid) can be increased; meanwhile, the Lactobacillus mucilaginosus FVT08 of Porro can obviously reduce the expression level of hepatic tissue inflammatory factors and improve hepatic tissue structure damage caused by obesity.