37 results about "Icr mice" patented technology

The present invention discloses six kinds of 3H-imidazo[4,5-c]pyridine-6-formyl-Met-AA-benzyl esters, preparation methods and antithrombotic activities of thereof, and further discloses anti-inflammatory activities on ICR mice, such that the present invention discloses double applications of the 3H-imidazo[4,5-c]pyridine-6-formyl-Met-AA-benzyl esters in anti-thrombus and anti-inflammation.

The invention discloses a preparation method of a 3D drug composite stent for a polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix. The preparation method comprises the following steps: firstly, preparing a circular acellular dermal matrix (ADM) stent from ICR rat full skin; secondly, grafting 6-arm amino polyethylene glycol (PEG) to GO to prepare polyethylene glycol modified graphene oxide (GO-PEG) under the condition that graphene oxide (GO) is catalyzed by EDC; thirdly, adsorbing quercetin (Que) to GO-PEG, and cross-linking prepared GO-PEG/Que to the surface of an ADM stent; and finally, preparing the 3D drug composite stent for the polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix (ADM-GO-PEG/Que). After the composite stent is transplanted to a diabetic skin wound, effects of transferring drug and healing the wound can be achieved.

The invention relates to an application of nicotinamide adenine dinucleotide to prepare drug for treating inflammatory pain. The modern pharmacological experiment proves that after injecting nicotinamide adenine dinucleotide to an ICR mouse, the nicotinamide adenine dinucleotide has analgesic effect to the inflammatory pain induced by formalin or complete Freund's adjuvant; it indicates that the nicotinamide adenine dinucleotide can be applied to treat inflammatory pain.

The invention relates to the field of gene engineering, and discloses a construction and an expression for two engineering bacteria of two human glucagon-like peptide-1 analogues, and a preparation method for the objective peptides. The objective peptides are formed by inserting objective sequence after double digestions, adding His labels at the front ends of fusion proteins, obtaining objective peptides by cutting the fusion proteins with acid via gaining the fusion proteins, and after an isoelectric precipitation, obtaining the objective peptides again through an affinity chromatography of a Ni agarose gel after an isoelectric precipitation. Freeze-dried objective peptides are dissolved in a PBS buffer solution, oral glucose tolerance test and determination of serum insulin concentration are carried out on normal ICR mice. The two analogues are modified peptides of human glucagon-like peptide-1. Experiments demonstrate that both the two peptides have effects for reducing blood glucose and can be used for treating type 2 diabetes mellitus. Furthermore, the invention also provides the preparation method for the peptides.

The invention discloses a synthetic peptide vaccine of B cell epitope based on TGF(transforming growth factor)-beta1 and application thereof, inhibits auxo-action of the cell factor TGF-B1-BB during the development process of liver fibrosis so as to inhibit the development of liver fibrosis to a some extent by constructing two epitope peptides of AY25 and LS30, coupling carrier proteins to synthesize an TGF-beta1 peptide vaccine, immunizing an ICR mice by intraperitoneal injection to induce the same to generate an anti-TGF-beta1 antibody, and neutralizing the cell factor TGF-B1-BB over expressed during CCL4 induced hepatic fibrosis process.

The invention provides application of an antibiotic substitute drug melatonin in preventing the infection of meningitis escherichia coli pathogens on child patients. Meningitis-type escherichia coli is separated and identified from muscovy duck cerebrospinal fluid, a meningitis model of 3-week-old ICR mice is constructed for the first time by utilizing the strain, and the successful construction of the mouse meningitis model is evaluated from the aspects of neurological symptom scoring, mortality difference statistics, the damage degree of a blood-brain barrier, expression of meningitis-related inflammatory factors and the like respectively. With antibiotics as references, the mice are treated with the melatonin and the four combined antibiotics respectively, one week later, the disease onset conditions of the meningitis mice are detected, results show that through the melatonin and the combined use of the antibiotics, the neurological symptoms of the meningitis mice can both be reduced, the survival rate of the meningitis mice is increased, integrity keeping of the blood-brain barrier is promoted by the melatonin, the invasion of inflammatory cells into the central nervous systemis reduced, the disease onset of the meningitis in the mice is prevented through the function of the brain-gut axis, and a new antibiotic substitute and treatment strategy are provided for preventingneonatal meningitis diseases.

The invention discloses an application of carbon monoxide molecules in inhibition on acute rejection after skin grafting. According to a large number of cell tests, the inventor discovers that after the carbon monoxide molecules are dissolved into a dimethylsulfoxide (DMSO) solvent and placed into an organism, CO can be continuously released, so the carbon monoxide molecules can serve as exogenous CO donors. The skin of ICR mice and BALB/C mice can serve as a donor and a receptor; an allogeneic gene acute rejection model can be established; exogenous CO is injected into the caudal veins of the mice; and early intervention and the test are performed, so that the carbon monoxide release molecules have inhibition effect on the acute rejection after skin grafting. The carbon monoxide release molecules are used for inhibiting the acute rejection after skin grafting; the concentration is controlled relatively easily and correctly; during long-term use, the carbon monoxide release molecules are added conveniently; and the carbon monoxide molecules can serve as an immuno-inhibitor with high specificity, low toxicity and high efficiency.

The invention provides a preparation method of a liver cancer animal model. The method comprises the following specific steps that (1), a 4-week-old ICR mouse is selected, observing and breeding are conducted for 2 weeks, HepG2 cells are subjected to culturing, passage and amplification in a DMEM culture medium mixed with 10% fetal bovine serum and then injected into the abdominal cavity of the ICR mouse through a syringe; (2), 2 mL of ascites is extracted after the mouse has obvious ascites, and added into a centrifuge tube, centrifugation is conducted, supernatant is discarded, 5 mL of a PBSsolution is added for resuspending and purging, then centrifugation is conducted for 5 minutes, and supernatant is discarded; (3), washing is conducted repeatedly 2-3 times according to the same method in step (2) through the PBS solution; (4), 5 mL of a PBS solution is added in sediment obtained after the supernatant is discarded in step (3) to blow away the cells, centrifugation is conducted for 3 minutes, erythrocyte of the upper layers of the supernatant and the sediment are taken out through a precise pipettor, and white sediment on the lower layer is retained. The effect of the preparedprimary liver cancer mouse model is greatly superior to an existing primary liver cancer mouse model building method at present, and compared with an existing model, the model is more realistic, practical, convenient and economical.

The invention provides a separation and culture method of mouse mesenchymal stem cells. The separation and culture method comprises the following steps: (1) an ICR mouse is anesthetized and killed; (2) thighbones and tibiae are taken and placed in ethyl alcohol of 75% to be soaked and disinfected; (3) washing is conducted for three times through PBS, and the epiphysis ends of the thighbones and the tibiae are cut off; (4) a culture medium containing double resistants is sucked through a needle tube of 10 ml to rush out bone marrow; (5) a percoll separating medium is used for centrifuging through a density gradient method, and a middle white layer is taken; (6) washing is conducted for two times through the PBS; (7) the cell activity is detected; (8) the cells are counted; (9) the cell morphology is identified; (10) detecting is conducted through an ELISA method; and (11) RT-PCR detection is conducted. The separation and culture method of the mouse mesenchymal stem cells is easy to operate, large in number of the obtained cells, high in survival rate and ideal, and provides the reliable cell resource for experiments.

The invention provides application of caffeic acid 3,4-dyhydroxy phenethyl ester (CADPE) to preparing a medicine for preventing colorectal cancer. The medicine is prepared from CADPE as an active component and a pharmaceutically acceptable carrier. The CAPDE is capable of effectively preventing early-stage occurrence of ICR (Institute for Cancer Research) mouse colorectal cancer induced by 1,2-dimethylhydrazine (DMH) and dextran sulphate sodium salt, has no conspicuous toxicity, and has the function mechanisms of inhibiting inflammatory cell invasion and infiltration and inhibiting NF-KappaB expression in monocyte, inhibiting expression of beta-catenin in aberrant crypt, reducing expression of cell proliferin PCNA and Cyclin D1, an anti-apoptoasis protein surviving and a cancer gene c-Myc,and inhibiting tumor vessel proliferation. Novel treatment means are provided for clinical prevention and treatment on colorectal cancer.

The invention provides a mouse skeletal muscle satellite cell separation and culture method. The mouse skeletal muscle satellite cell separation and culture method comprises following steps: 1, mice are killed through intraperitoneal injection of pentobarbital sodium, and are immersed in 75% alcohol for disinfection; 2, the mice are fixed on plates with the venter posterior surfaces upward, hind limb skin is peeled under aseptic conditions, and thighbone muscle tissues are collected; 3, in an aseptic operation bench, PBS washing is carried out for three times, surface blood vessels, tendons, and connective tissues are removed; 4, the processed tissues are cut into pieces, and are subjected to digestion with preheated trypsin and IV type collagenase respectively; 5, digestion is stopped, centrifugation is carried out, and an obtained supernatant is removed; 6, a cell filtrate is collected, and trypan-blue drying detection is adopted to detect cell activity; 7, after cell counting, a complete medium is adopted for resuspension inoculation into a coated culture flask for culture at 37 DEG C at 5% CO2 environment; 8, differential attachment method is adopted to remove fibrocytes; 9, cell morphology is carried out; 10, ELISA method detection is carried out; and 11, RT-PCR detection is carried out. The mouse skeletal muscle satellite cell separation and culture method is simple in operation, and is high in survival rate; the number of obtained cells is large; the mouse skeletal muscle satellite cell separation and culture method is an ideal method, and is capable of providing experiments with reliable cell resources.

The invention relates to a building method of an acute hyperuricemia mouse model, and belongs to the technical field of model building. According to the method, inosine enterocoelia is injected into an ICR mouse at an injection amount of 400 to 500 mg/kg, thus obtaining the acute hyperuricemia mouse model. According to the building method, an acute hyperuricemia animal model is firstly built by inosine for an ICR mouse serving as a common experimental animal in a biomedical research. The model has a typical illness representation; quantity indexes have statistical significance; the model is a simple, feasible, reliable and practical technology; furthermore, the invention provides a hypeluricemia animal model obtained by simulating a human uric acid metabolism path, and the hypeluricemia animal model has an important significance for developing researches on relevant diseases.

The invention belongs to the field of pharmacy, and particularly relates to a 3-deoxy-5-hydroxy-1-amino carbohydrate compound with a general formula shown in the specification and an application of the 3-deoxy-5-hydroxy-1-amino carbohydrate compound, and R is described in the claims and the specification. The protected 3-deoxy-5-hydroxycyclohexanone is synthesized through a stereoselective reduction method and a position selective reduction method, and the compound has a novel carbohydrate skeleton. The preparation method comprises the following steps: enabling a compound to react with small molecular amines by virtue of a reductive amination method, so as to obtain a corresponding target object protected by a protecting group, and finally, carrying out a deprotecting group reaction, so asto obtain an amino carbohydrate target object with 3-deoxy-5-hydroxyl structural characteristics. The target object has good inhibitory activity on alpha-glycosidase, and has a significant inhibitoryeffect on blood glucose rise of normal ICR mice after sucrose loading, and the target object can be used for further development of hypoglycemic drugs.

The invention belongs to the technical field of new species of vertebrates, and discloses a detection method for oxidative stress functions of different organs of mice. The clean male ICR mice with the weight of 20 g are selected, the mice are randomly grouped, and CdSe/ZnS QDs establishes a poisoned mouse model through tail intravenous injection; low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD605 are prepared, and control groups, low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD525 are prepared; all the control groups are treated by normal saline; the exposure times of the dosage groups are 11 days, 22 days and 33 days respectively. According to the method, by observing the influence of the quantum dots on genes and proteomes relevant to DNA impairment of the mice and liver and kidney antioxidase, the molecular toxicity effect of the CdSe/ZnS quantum dots on the animal bodies is further disclosed, and basic materials are provided for toxicology invention and bio-safety evaluation of the quantum dots.

The invention discloses an application of caffeic acid phenethylester for preparing a pharmaceutical for reinforcing a learning and memory ability. The effective ingredient of the pharmaceutical for reinforcing the learning and memory ability comprises the caffeic acid phenethylester. A grouping comparison test is executed to ICR mice, and the error frequency of the incubation period and the observation period of the normal ICR mice is counted through a step-down test and a dark avoidance test, so the application field of the caffeic acid phenethylester is broadened.

The invention belongs to the technical field of experimental animal breeding, relates to a method for breeding ICR mice in high blastocyst formation rate and aims to solve the problem of low blastocyst formation rate in existing in-vitro fertilization. The method includes steps: selecting a multiparous ICR female mouse as a line establishment female mouse, subjecting to mating line establishment with a line establishment male mouse to produce F1-generation mice, and by early sex determination, selecting and keeping female mice as F1-generation ICR female mice for backcross; subjecting the F1-generation ICR female mice with the line establishment male mouse to backcross to produce F2-generation mice, and by early sex determination, selecting and keeping female mice as F2-generation ICR female mice for backcross; subjecting the F2-generation ICR female mice with the line establishment male mouse to backcross to produce F3-generation mice, and by early sex determination in the F3-generation mice, screening out F3-generation female mice as the ICR mice in high blastocyst formation rate. By the method, in-vitro 2-cell development block can be effectively overcome, and the blastocyst formation rate is increased.

The invention discloses a novel hypolipidemic effect of piperine. The piperine significantly reduces serum total cholesterol TC, total triglyceride TG and low density lipoprotein cholesterol LDL-C levels in lipid-loaded ICR mice; the piperine concentration-dependently increases cholesterol efflux from human THP-1 macrophages, specifically upregulates ABCA1 protein levels, and acts similarly to calpeptin, a calpain inhibitor. The upregulation of the protein levels of ABCA1 by the piperine is achieved by inhibiting the activity of total calpain in cells. The piperine has the beneficial effect ofproviding a theoretical basis for further development of the piperine as a blood fat reducing medicament or a health food.

A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation and related to suppressing thymic cancer, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, immunogenic and apoptotic genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation to be accurately detected by preventing the interference of confounding variables.

The invention discloses a method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF) and relates to the field of medical technology. The method includes the steps of adopting ICR mice to carry out animal experiments, utilizing a Morris water maze method to measure the influence of the procyanidins from the lotus seed pots on learning capacity and memory capacity of the mice and brain antioxidase systems of the mice due to the ELF-EMF and measure the content or the activity of lipid peroxidation products, nitric oxide and nitric oxide synthase in brain tissues and hippocampus of the mice, measuring the influence of the procyanidins from the lotus seed pots on the cell survival rate, cell survival forms, the content of antioxidase, the content of reactive oxide radicals, the content of the nitric oxide and the content of nitric oxide synthase through research of an in-vitro cultured hippocampal neuron cell damage model caused by the ELF-EMF. The result proves that the procyanidins from the lotus seed pots have a remarkable preventive effect on learning and memory deterioration due to the brain oxidative stress injury caused by the ELF-EMF in vitro and in vivo.

A method for detecting genes sensitive to high-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to high-level ionizing radiation discovered in a carcinogenic entity and verified in a normal entity are detected, by subjecting a cancerous AKR/J mouse and a normal ICR mouse to high-level radiation. Thymus is collected therefrom and fatty acid metabolism-related genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.