Method for breeding ICR mice in high blastocyst formation rate

A breeding method, mouse technology, applied in animal husbandry, etc., can solve the problems of complex experimental process, high cost, poor operability, etc., and achieve the effect of increasing gene homozygosity, increasing gene homozygosity, and increasing blastocyst formation rate

Active Publication Date: 2019-06-28
TAIZHOU ENZE MEDICAL CENT GROUP
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Problems solved by technology

For example, the Chinese patent application (publication number: CN105647853A) discloses a method for improving the developmental quality of female embryos attached to in vitro fertilization by adding retinoic acid in the experimental group to improve the blastocyst rate in th

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  • Method for breeding ICR mice in high blastocyst formation rate
  • Method for breeding ICR mice in high blastocyst formation rate

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Embodiment Construction

[0020] The technical solutions of the present invention will be further specifically described below through specific embodiments and accompanying drawings, but the present invention is not limited to these embodiments.

[0021] combine figure 2 As shown, the specific breeding method of the present invention is as follows: select 8-week-old ICR male mice with a plug-seeing rate higher than 80% as male mice for establishing a line, and select multi-parous ICR female mice for pairing to establish a line to produce F1. It is best to select ICR dams with a litter size of ≥ 10 and 10-15 weeks of age for ICR dams, who have experience in giving birth and strong reproductive ability; In the F1 generation, early gender identification was carried out by comparing the size of the reproductive process and the distance between the anal process, and the offspring male mice with larger reproductive processes and longer distances between the anus and the reproductive process were eliminated,...

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Abstract

The invention belongs to the technical field of experimental animal breeding, relates to a method for breeding ICR mice in high blastocyst formation rate and aims to solve the problem of low blastocyst formation rate in existing in-vitro fertilization. The method includes steps: selecting a multiparous ICR female mouse as a line establishment female mouse, subjecting to mating line establishment with a line establishment male mouse to produce F1-generation mice, and by early sex determination, selecting and keeping female mice as F1-generation ICR female mice for backcross; subjecting the F1-generation ICR female mice with the line establishment male mouse to backcross to produce F2-generation mice, and by early sex determination, selecting and keeping female mice as F2-generation ICR female mice for backcross; subjecting the F2-generation ICR female mice with the line establishment male mouse to backcross to produce F3-generation mice, and by early sex determination in the F3-generation mice, screening out F3-generation female mice as the ICR mice in high blastocyst formation rate. By the method, in-vitro 2-cell development block can be effectively overcome, and the blastocyst formation rate is increased.

Description

technical field [0001] The invention relates to a method for cultivating ICR mice with a high blastocyst formation rate, belonging to the technical field of experimental animal breeding. Background technique [0002] Mice are important model organisms for medical research and have been widely used in the field of life science research. In particular, humanized mouse models are living surrogate models for studying human diseases, and have great advantages in elucidating pathogenesis and drug screening. and broad application prospects, and the establishment of these models needs to obtain high developmental potential embryo engineering in vitro as the basis. "Test-tube baby technology" is a major invention of human reproductive medicine in the 19th century, and "test-tube baby technology" has strict requirements on the environment, culture medium, etc. In order to ensure a stable success rate and the health of offspring, a sensitive and Highly reliable inspection system. [...

Claims

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Application Information

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IPC IPC(8): A01K67/02
Inventor 杨欢利诸溢扬陆文昊吴为玲李真法蔡娇娇陈辉波
Owner TAIZHOU ENZE MEDICAL CENT GROUP
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