A kind of cultivation method of high blastocyst formation rate icr mice

Abstract

The invention relates to a method for cultivating ICR mice with a high blastocyst formation rate, belonging to the technical field of experimental animal breeding. In order to solve the problem of low blastocyst formation rate in the existing in vitro fertilization, a method for cultivating ICR mice with a high blastocyst formation rate is provided, which includes selecting ICR females that have been born as female mice for establishing a line, and making them and males for establishing a line Mice were paired to establish a line to produce F1 generation mice. Through early sex identification, female mice were selected as F1 generation ICR female mice for backcrossing; F1 generation ICR female mice were backcrossed with established male mice to produce F2 generation mice. Through early gender identification, the female mice were selected as the F2 generation ICR female mice for backcrossing; the selected F2 generation ICR female mice were backcrossed with the established male mice to produce F3 generation mice, and the F3 generation mice passed the early stage F3 generation female mice were selected as ICR mice with high blastocyst formation rate by sex identification. The invention can effectively overcome the 2-cell development block in vitro and improve the blastocyst formation rate.

Description

technical field [0001] The invention relates to a method for cultivating ICR mice with a high blastocyst formation rate, belonging to the technical field of experimental animal breeding. Background technique [0002] Mice are important model organisms for medical research and have been widely used in the field of life science research. In particular, humanized mouse models are living surrogate models for studying human diseases, and have great advantages in elucidating pathogenesis and drug screening. and broad application prospects, and the establishment of these models needs to obtain high developmental potential embryo engineering in vitro as the basis. "Test-tube baby technology" is a major invention of human reproductive medicine in the 19th century, and "test-tube baby technology" has strict requirements on the environment, culture medium, etc. In order to ensure a stable success rate and the health of offspring, a sensitive and Highly reliable inspection system. [...

AI Technical Summary

Problems solved by technology

For example, the Chinese patent application (publication number: CN105647853A) discloses a method for improving the developmental quality of female embryos attached to in vitro fertilization by adding retinoic acid in the experimental group to improve the blastocyst rate in the in vitro fertilization experiment. , the experimental process is too complicated and costly, and the operability is poor, and it is precisely because of the problem that the blastocyst rate is affected by the 2-cell arrest phenomenon that it needs to be reprocessed in vitro

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