35 results about "Cellular development" patented technology

This invention provides gels and matrices having a rigidity in the range of 150-750 Pa, methods of manufacturing same, and method of preserving a mesenchymal stem cell population or studying mesenchymal stem cells, comprising same.

Methods are described that bias cells, such as potent and multipotent stem cells, by transfection with a nucleic acid sequence, to differentiate to a desired end-stage cell or a cell having characteristics of a desired end-stage cell. In particular embodiments, human neural stem cells are transfected with vectors comprising genes in the homeobox family of transcription factor developmental control genes, and this results in a greater percentage of resultant transformed cells, or their progeny, differentiating into a desired end-stage cell or a cell having characteristics of a desired end-stage cell.

The invention provides methods of determining the aggressiveness, prognosis and response to therapy for particular cancers, which include comparing the expression levels of one or a plurality of differentially expressed genes from one or more 5 functional metagenes, including a carbohydrate / lipid metabolism metagene, a cell signalling metagene, a cellular development metagene, a cellular growth metagene, a chromosome segregation metagene, a DNA replication / recombination metagene, an immune system metagene, a metabolic disease metagene, a nucleic acid metabolism metagene, a post-translational modification metagene, a protein 10 synthesis / modification metagene and a multiple networks metagene. The method disclosed herein may be particularly suitable as a companion diagnostic for cancer therapies.

The invention relates to a space load predication method with consideration of a cellular development degree. The method comprises: a power geographic information system (GIS) is established, and integration of land information, a power supply range of a 10-kV feeder line, and classification load data is realized; cellular units are generated in the GIS in the power supply range of the 10-kV feeder line; changing situations of classification load densities are described by using a logistic function, and regular curves corresponding to all classification load density development trends are generated respectively; development degrees of classification load densities in all cellular units in a current year are determined, and thus positions of classification load densities in all cellular units in a current year on the load density development rule curves of the classes are found; and a cellular load value of a target year is predicted, a classification load density index in the cellular unit at the target year is determined by combining the classification load density development rule curves according to the classification load density development degrees in all cellular units at the current year, and then multiplication by areas corresponding to all kinds of loads in the cellular units to realize prediction of cellular load values.

Methods and apparatuses for extracting, modifying and returning cells to / from a patient to treat the patient. These apparatuses and methods may use nanostraws to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into the cells rapidly and efficiently. The methods and apparatuses described herein are repeatedly capable of delivering biologically relevant cargo into cells with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency, including in a continuous or semi-continuous manner.

The invention discloses peanut cookie and a processing method thereof. The peanut cookie is prepared by the following raw materials in parts by weight: 60-80 parts of flour, 10-15 parts of peanut, 2-3 parts of baking soda, 3-4 parts of piny flower agent, 6-8 parts of cream, 6-8 parts of grape juice, 2-4 parts of mulberry leaves, 2-4 parts of cortex albiziae, 2-3 parts of semen cassiae, 1-2 parts of mint, 2-3 parts of white paeony root, 1-3 parts of anise, 2-3 parts of poria, 1-4 parts of panax quinquefolium, 6-8 parts of custer sugar and 6-8 parts of nutrition additives. According to the peanut cookie and the processing method of the peanut cookie, provided by the invention, the processing method is simple in technology, is reasonable in recipe and is suitable for industrialized production; meanwhile, as multiple traditional Chinese medicine ingredients, such as the mulberry leaves, the cortex albiziae and the semen cassiae, having a healthcare function are added in the raw materials, the peanut cookie has the efficacies of promoting the growth and development of a human body and promoting the cell development.

The invention relates to a construction method of an STAT3 mitochondrial localization conditional gene knock-in mouse model. The method comprises the following steps: firstly, constructing an STAT3 mitochondrial localization conditional gene knock-in recombinant targeting vector, transfecting the recombinant targeting vector to fertilized ova of a donor mouse, and then transplanting the donor mouse fertilized ova into the uterus of a fake pregnant mother mouse to produce a chimeric mouse; mating the chimeric mouse with a C57BL / 6J mouse to obtain an F1 generation; mating the F1 generation witha mouse of tissue-specific expression Cre recombinase to obtain a double-hybrid mouse; and are intercrossing the double-hybrid mouse and an STAT3<f / +> or STAT3<f / f> mouse, and inducing the obtained offspring by tamoxifen to obtain the STAT3 mitochondrial localization conditional gene knock-in mouse model. According to the mouse model disclosed by the invention, the STAT3 gene is expressed in specific tissues and cell types, and an ideal model is provided for researching the action mechanism of STAT3 in specific cell development and differentiation, body metabolism and tumor occurrence and development.

The invention discloses a method for separating Eucheuma seaweed protoplast and culturing a regenerated plant, comprising the following steps of: according to the totipotency of the seaweed cell development, decomposing a eucheuma tissue by using seaweed tool enzyme, separating out cell protoplast, obtaining the somatic cell and cell line of the regenerated cell wall, and then culturing the cell to obtain the regenerated plant. In the method, the somatic cell protoplast is adopted to directly carry out seedling without culturing the mature alga of eucheuma; the regenerated cell wall of the somatic cell protoplast is converted into the somatic cell; compared with the technology of amphigony seedling, the method shortens the seedling period without the amphigony process of reproductive cell; moreover, the method has high seedling breeding efficiency and is suitable for commercial seedling production.

Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip.

The invention provides a method for searching for a target gene of a Wnt signal pathway in a process of differentiating embryonic stem cells into primordial germ cells and relates to the technical field of biology. According to the method, firstly, target gene prediction is carried out on a key transcription factor TCF7L2 of the Wnt signal pathway, GO function annotation is carried out on the predicted target gene, and a conservative target gene which is involved in germ cell development and stem cell differentiation is screened out as a to-be-selected target gene. In-vivo / in-vitro experiments are carried out to verify the regulation and control effect of a Wnt signal on the transcription level of the to-be-selected target gene, and when a transcription factor binding site in a promoter of the to-be-selected target gene is lost, whether or not the Wnt signal has a regulation effect on the transcription level of the to-be-selected target gene is detected; the binding effect of a beta-Catenin / transcription factor complex and the to-be-selected target gene is verified through ChIP-qPCR in order to determine whether or not the to-be-selected target gene has a targeting response effect on the Wnt signal. The method is simple and feasible.

The invention provides a tissue preservation solution and an application thereof. The tissue preservation solution comprises a basic culture solution and additive factors, the addition factor at least comprises the following components with final concentration: N-acetylcysteine with the concentration of 0.2 to 5 [mu]M; indole-3-lactic acid with the concentration of 100 to 1000 [mu]M; Y-27632 with the concentration of 10 to 100 [mu]M; GDF11 with the concentration of 10 to 100 ng / ml; a penicillin-streptomycin mixed solution with 1-5 *; and Primocin with the concentration of 0.5 to 5 mg / ml. The tissue preservation solution is applied to preservation of in-vitro tissues before primary culture. The preservation method comprises the following steps: completely immersing tissues in vitro into the tissue preservation solution, and preserving at 2-8 DEG C. According to the tissue preservation solution, the effective preservation period of normal tissues after in-vitro treatment exceeds 12 hours, the effective preservation period of tumor tissues and cells after in-vitro treatment exceeds 48 hours, the tissues preserved for a long time can still keep high cell viability and keep cell development characteristics, and the success rate of primary cell culture is remarkably increased. In addition, the tissue preservation solution disclosed by the invention has important significance on primary culture specimens needing to be preserved for a long time, especially precious clinical specimens.

The invention relates to a dual-induction mCreER system capable of tracking cell differentiation and development. The dual-induction mCreER system comprises mCreER protein and mTEVp protein, wherein the mCreER protein and the mTEVp protein are connected with CD8 positioned on a cell membrane through FRB protein and FKBP protein, and are correspondingly fixed on the cell membrane; and when rapamycin is added, FKBP protein and FRB protein approach each other and are combined to form a complex, cutting of mTEVp protein is induced, mCreER is released from plasma membrane and enters a nucleus under the action of tamoxifen to mediate loxP site recombination, and controllability of recombination time is achieved. According to the dual-induction mCreER system, the Cre recombinase can enter a cell nucleus for recombination only under the induction condition, and the spontaneous recombination phenomenon of a traditional CreER system is avoided. The mCreER is membrane protein, the relative expression quantity is relatively low, and after the mCreER is induced to enter the cell nucleus, the off-target phenomenon of Cre recombinase can be reduced, the cytotoxicity is reduced, and the tissue specificity is improved; and the tissue cells can be tracked more strictly and accurately, and a new thought is provided for cell development, tissue regeneration and disease treatment.

Plant growth enhancing mixture and method of selectively timing the application of same during the development of crop plants or other plants to positively augment cell number increase and cellular development of crop plants or other plants to enhance development and / or productivity of the economic portion of the crop plant or other plant. Application of the plant growth enhancing mixture at flowering enhances both weak flowers and normally strong flowers. The plant growth enhancing mixture and method of application have also been shown to impart varying disease resistance to the treated crop or other plants. The plant growth enhancing mixture and method of application also increases the depth and strength of rooting for greater access and transport of water and nutrients for growth and productivity of the crop plant.

Conditionally-immortalized human CNS progenitor cell lines are provided. Such cell lines, which may be clonal, may be used to generate neurons and / or astrocytes. Such cell lines and / or differentiated cells may be used for the development of therapeutic agents to prevent and treat a variety of CNS-related diseases. Such cell lines and / or differentiated cells may also be used in assays and for the general study of CNS cell development, death and abnormalities.

The invention discloses a preparation method for a plant mutant. The preparation method comprises the following steps that T1, a CRISPR / cas9 gene knockout vector is constructed, and a CYP78A13 gene isknocked out; T2, agrobacterium is transformed; T3, gene knockout detection is carried out; and T4, phenotype identification is carried out on a transgenic plant. According to the preparation method for the plant mutant, a rice cell nucleus fertility gene serves as a target gene, gene knockout is carried out by using CRISPR / cas9 gene silencing, the function is lost after gene silencing, a cell isdeveloped into a complete plant, but the complete plant does not have pollen which is developed to have normal functions, and the plant mutant can be taken as a research object and used for creating asterile line.

The invention provides a granulocyte and monocyte marking system. The marking system comprises an Ms4a3 gene, an IRES-Cre recombinase and a LoxP-Stop-LoxP-reporter reporting system. The Ms4a3 gene isspecifically expressed by granulocyte and monocyte precursors, and the sequence of the gene is shown as SEQ ID NO. 1. The invention further provides a construction method and a detection method of themarking system. The invention further provides wide application of the system in the aspects of hematopoietic system development, immune cell development and functions, oncology research and the like.

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.

The invention belongs to the technical field of biology, and discloses a method for extracting cotton fiber cell early development RNA, which comprises the following steps: taking a plurality of ovaryin different development stages, and taking complete ovules; putting the ovules into a lysis solution, vacuumizing, centrifuging and taking a supernate; filtratioffing the supernatant, adding absolute ethyl alcohol into the filtered supernatant to precipitate nucleic acid, collecting the obtained solution, adding a deproteinization solution RW1 to wash protein, adding a DNaseI working solution todigest DNA, adding the deproteinization solution RW1 and a rinsing solution RW to wash other impurities except the nucleic acid, and collecting the eluted RNA solution; and finally, performing RNA concentration and purity detection. The method is suitable for any cotton variety. The method can be used for rapidly extracting the cotton fiber cell development early-stage specific RNA which is difficult to separate in -1DPA, 0DPA, 1DPA, 2DPA, 3DPA, 4DPA and 5DPA periods, and meets the requirements of transcriptional library building.

The invention provides a marker for evaluating hepatic lineage cell maturity, a biomics kit and a construction method. According to the invention, a microRNA set and a gene set which have time sequence characteristics and have a regulatory relationship in cell development are elaborated from a new perspective for the first time, a transcription factor-microRNA-target gene regulatory network related to hepatocyte differentiation and maturation regulation is established, and a gene tag and a microRNA tag of hepatolineage specificity are obtained, wherein these tags or combinations thereof can be used as potential new markers for hepatic lineage cells. The invention also provides a biomics kit for the hepatic lineage cells, and a new scheme is provided for large-scale amplification of stable hepatic lineage cells and monitoring of in-vitro culture states of the hepatic lineage cells.

The present invention provides an isolated interaction polynucleotide that contains a tag sequence and two or more genetic elements. The present invention also provides a method for identifying an interaction between two or more genetic elements. One embodiment of the present invention provides a method for analyzing and identifying the interaction between two or more genetic elements under various culture conditions or as a result of differentiation or pathological cellular development, wherein the genetic elements interact to stimulate or inhibit cell growth.

The invention provides a tissue preservation solution and its application. The tissue preservation solution includes basal culture solution and added factors; the added factors include at least the following final concentration components: N-acetylcysteine, 0.2-5 μM; indole-3-lactic acid: 100-1000 μM; Y-27632 10- 100μM; GDF11 10-100ng / ml; Penicillin-Streptomycin Mixture, 1-5×; Primocin, 0.5-5mg / ml. The tissue preservation solution is used in the preservation of isolated tissues before primary culture. The preservation method includes: completely immersing the tissue in the above-mentioned tissue preservation solution after being isolated, and preserving it at 2-8°C. The tissue preservation solution of the present invention has an effective storage period of more than 12 hours for normal tissues after isolation, and an effective storage period of tumor tissues and their cells for more than 48 hours after isolation, and tissues preserved for a long time can still maintain a high Cell viability and maintain cell development characteristics, significantly improve the success rate of primary cell culture. In addition, the tissue preservation solution of the present invention is of great significance to primary culture specimens that need to be preserved for a long time, especially precious clinical specimens.

Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1a or Lif and disruption of the Sdf-1a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.

The present invention relates to a method for modeling the process of gene translation in cells, comprising the following steps: obtaining whether each gene is expressed at each discrete time point according to the experimental data of the species; querying the genes at each discrete time point, if the gene is expressed, Then initialize; otherwise, do not initialize; compare the number of mRNA copies corresponding to each gene at each time point with the number of mRNAs at the previous time point to obtain the number of initializations in various mRNAs; calculate the total initialization speed of mRNA molecules; calculate ribosomal molecules The total elongation speed on the corresponding mRNA; the probability of events is obtained according to the total initialization speed and the total elongation speed; an event is randomly selected according to the weight of the event probability; and the states of various molecules are updated. The invention adopts the principle based on a completely asymmetric and simple repulsion process, and considers the dynamic changes of gene expression at the same time, so that the translation process presents a time series feature, which is closer to the essence of cell development, and is suitable for studying the dynamic development properties of cells.

The invention relates to construction of an SV40LT gene-mediated megakaryocyte model and a cell bank of megakaryocyte, belonging to the field of medical research. The construction is mainly characterized in that an SV40LTag-pcDNA3.1 (-) recombinant is constructed from T4DNA ligase, BamHI, pcDNA3.1 (-) DNA and SV40LTag DNA by virtue of conventional means; the recombinant is purified by virtue of competent escherichia coli; the recombinant is introduced into megakaryocyte cultured in vitro through a lipofection transfection method, so that the recombinant is integrated with the DNA of the cell; a cell screened by virtue of G418 and containing a positive recombinant is subjected to subculture and enlarged cultivation; a cell which is consistent with the biological properties of an immortalized cell in cellular morphology, cell growth curve, karyotype, nude mice carcinogenicity test, detection of SV40 large T gene in transfection cell DNA, determination of an mRNA expression product and a DNA sequence determination result is screened out as a megakaryocyte model, and is cryopreserved in liquid nitrogen, so as to construct an SV40LT gene-mediated megakaryocyte model and the cell bank of megakaryocyte; therefore, a method for long-term in vitro subculture of the megakaryocyte is established, and the method is applicable to the in vitro research of development, function and pathogenic mechanism of the megakaryocyte.

Plant growth enhancing mixture and method of selectively timing the application of same during the development of crop plants or other plants to positively augment cell number increase and cellular development of crop plants or other plants to enhance development and / or productivity of the economic portion of the crop plant or other plant. Application of the plant growth enhancing mixture at flowering enhances both weak flowers and normally strong flowers. The plant growth enhancing mixture and method of application have also been shown to impart varying disease resistance to the treated crop or other plants. The plant growth enhancing mixture and method of application also increases the depth and strength of rooting for greater access and transport of water and nutrients for growth and productivity of the crop plant.

The invention provides a construction method of a humanized immune system mouse with NK cell and ADCC capacity, which is characterized by comprising the step of artificially supplementing exogenous cytokines IL-15. After exogenous human cytokines are supplemented, the development level of NK cells in a mouse body of a humanized immune system is greatly improved, the level of a corresponding NK cell subset in peripheral blood of a normal person is achieved, and corresponding functions are achieved.

This technology belongs to the field of biotechnology, and discloses a method for extracting early RNA of cotton fiber cell development. Several ovaries of different development stages are taken, and complete ovules are taken; Clear; filter the supernatant, add absolute ethanol to the filtered supernatant to precipitate nucleic acid, collect the obtained solution, then add protein-removing solution RW1 to wash the protein, add DNase I working solution to digest DNA, then add protein-removing solution RW1 and rinse Liquid RW was used to wash other impurities except nucleic acid, and then the eluted RNA solution was collected; finally, the RNA concentration and purity were tested. The present invention is applicable to any cotton variety. It can quickly extract specific RNAs in the early stages of cotton fiber cell development that are difficult to separate at ‑1DPA, 0DPA, 1DPA, 2DPA, 3DPA, 4DPA, and 5DPA, and meet the requirements for transcriptional library construction.

The invention discloses a cell development map of mandible tissues of human four-month-old embryos. Comprising 19 cell populations including endothelial cells, Schwann cells, proliferating cells, satellite cells, muscle cells, red blood cells, myoblasts, transformed cells 3, transformed cells 1, mesenchymal stem cells, monocytes, osteoclasts, transformed cells 2, neural crest cells, osteoblasts, epithelial cells, chondrocytes, pericytes and tendons. The invention further discloses a construction method of the cell development map of the mandible tissue of the human four-month-old embryo, and the cell development map of the mandible tissue of the human four-month-old embryo is constructed by carrying out single cell sequencing on the mandible tissue of the human four-month-old embryo, carrying out UMAP dimension reduction grouping and annotating a cell population by utilizing a Marker gene. The invention provides a complete and comprehensive cell development map of mandible tissues of human four-month-old embryos, and provides new basic data for researching the growth and development of mandible.

The present invention relates to a plant growth regulator composition comprising a first component involved in transcriptional level regulation of cell development in response to cell development, a second component that induces a promoter response in response to cell division, and a third component that provides a base material for vital activity in which the first component and the second component are involved. The plant growth regulator composition can act on pedicels or inflorescence tissues of rosa benthamiana, so that the rosa benthamiana recovers a normal state with commercial value.