Method for separating Eucheuma seaweed protoplast and culturing regenerated plant

A technology for protoplast and plant regeneration, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of occupying a large area of ​​water, unable to improve varieties, unstable seed breeding and production, etc., and achieve breeding efficiency. The effect of high and shortened seedling cycle

Active Publication Date: 2011-08-10
OCEAN UNIV OF CHINA
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Problems solved by technology

However, due to the large water area occupied by this method, and the environmental impact of the algae in summer due to high water temperature and high precipitation, the salinity is too low, the seed breeding and production are unstable.
In addition, the body wall of Eucheuma seaweed has a thick gelatinous layer, so it is impossible to directly apply genetic transformation technologies such as electroporation for species improvement.

Method used

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  • Method for separating Eucheuma seaweed protoplast and culturing regenerated plant
  • Method for separating Eucheuma seaweed protoplast and culturing regenerated plant
  • Method for separating Eucheuma seaweed protoplast and culturing regenerated plant

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Embodiment Construction

[0019] The present invention will be further described through specific embodiments below in conjunction with the accompanying drawings.

[0020] The experimental material of this embodiment is Eucheuma ( Eucheuma denticulatum ).

[0021] 1. Protoplast isolation method

[0022] Take 1-2 grams of Eucheuma, grind or cut it into small tissue pieces or small branches, use marine shellfish (balone) digestive glands to extract and purify, and make seaweed according to the method of biochemically purifying proteins (enzymes) For tool enzymes, add 2 M glucose as an osmotic agent, and immerse small tissue pieces or small branches in the seaweed tool enzyme solution at 20-25°C for 2-3 hours, stirring with a glass rod during this period. Use a 300-mesh sieve to filter the cell mixture to remove undigested cells, cell clusters, and debris; take the supernatant and centrifuge at 800 rpm for 10 minutes to make the single cells (protoplasts) sink, and the cell debris remains in the super...

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Abstract

The invention discloses a method for separating Eucheuma seaweed protoplast and culturing a regenerated plant, comprising the following steps of: according to the totipotency of the seaweed cell development, decomposing a eucheuma tissue by using seaweed tool enzyme, separating out cell protoplast, obtaining the somatic cell and cell line of the regenerated cell wall, and then culturing the cell to obtain the regenerated plant. In the method, the somatic cell protoplast is adopted to directly carry out seedling without culturing the mature alga of eucheuma; the regenerated cell wall of the somatic cell protoplast is converted into the somatic cell; compared with the technology of amphigony seedling, the method shortens the seedling period without the amphigony process of reproductive cell; moreover, the method has high seedling breeding efficiency and is suitable for commercial seedling production.

Description

technical field [0001] The invention relates to a method for cultivating algae, in particular to a method for isolating and cultivating protoplasts of Euglena genus seaweed and regenerating plants. Background technique [0002] The natural reproduction of Eucheuma genus seaweed is mainly carried out by the mode of tetrasporangium and fruit sporangia. At present, in the cultivation and production of Eucheuma genus seaweed, it is mainly cultivated by cutting and regenerating. However, due to the large water area occupied by this method, and the environmental impact of the algae body due to high water temperature and high precipitation in summer due to low salinity, the breeding and production of seedlings is unstable. In addition, the body wall of Eucheuma seaweed has a thick gelatinous layer, so it is impossible to directly apply genetic transformation techniques such as electroporation to improve species. Contents of the invention [0003] In view of the problems referre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘涛张偲金月梅
Owner OCEAN UNIV OF CHINA
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