43 results about "CELL DEBRIS" patented technology

The invention discloses an extraction preparation method and application of algae micromolecule PDRN, and relates to the technical field of biology. The invention establishes a process for extracting PDRN from microalgae, which comprises the following steps: carrying out cell disruption on microalgae liquid, and removing cell debris to obtain filtrate; the filtrate is subjected to DNA dissolution, DNA separation and DNA purification, and a PDRN solution is obtained; wherein the dry weight of the microalgae in the algae liquid is 0.1 to 0.333 g / mL. Through process optimization, the extraction efficiency and purity of the microalgae PDRN are improved, and the extracted PDRN has excellent anti-aging and anti-inflammatory effects.

The invention relates to the technical field of medical detection, in particular to a microneedle array-containing S100A9 sample sampling and detecting device which comprises a base, a limiting column and a color developing assembly, the limiting column is fixedly connected to the top end of the base, and a working sliding groove for inserting the color developing assembly is formed between the limiting column and the base; a sampling groove is formed in the top end of the base, the inner bottom wall of the sampling groove is fixedly connected with a microneedle assembly used for puncturing the skin of a patient for sampling, and the top of the microneedle assembly communicates with a pretreatment assembly used for filtering an extracted sample; driving force is provided for the pretreatment assembly in the process that the color developing assembly is inserted into the working sliding groove, and the filtered sample is input into the color developing assembly to be detected. According to the invention, convenient sampling is realized through the microneedle assembly, and a sample is pertinently filtered by using the pretreatment assembly, so that macromolecular interference impurities such as cell debris and hemoglobin are effectively removed, the interference on S100A9 detection is reduced, and the accuracy and reliability of S100A9 detection are improved.

The invention discloses a sleep-promoting nasal spray based on mesenchymal stem cell-derived exosomes and preparation and application thereof.The preparation method comprises the steps that 1, mesenchymal stem cells separated from umbilical cord tissue are cultured to P3-P5 generations, cell culture supernate is collected, cells are digested, and then cell debris and macromolecular impure protein are removed through pretreatment; (2) filtering and concentrating the pretreated cell culture supernatant and replacing a part of solution; (3) carrying out degerming filtration on the solution obtained in the step (2) through a 0.22 [mu] m filter membrane to obtain an MSC-Exos solution; and (4) adding a stabilizer with a proper concentration into the MSC-Exos solution to obtain a compound MSC-Exos solution, namely the sleep-promoting nasal spray. The product is a cell-derived natural active substance, can significantly improve sleep, and does not involve any pharmaceutical ingredient.

The invention discloses a purification method of cord blood mesenchymal stem cell source exosome, and relates to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out pH regulation pretreatment on cell culture supernate through a technical path of combining gradient centrifugation and membrane filtration, and sequentially removing cell debris and large-particle impurities by adopting multi-stage differential centrifugation; then carrying out selective protein precipitation by utilizing polyethylene glycol with specific molecular weight, and realizing fine separation of the exosome by combining sucrose-meglumine diatrizoate density gradient centrifugation; and finally, terminal purification is completed under low-temperature and low-pressure conditions through a polyethersulfone membrane filtration system with an optimized pore size. According to the method, centrifugal parameters, reagent concentration and filtering conditions are precisely regulated and controlled, so that the particle size of the obtained exosome is intensively distributed in the range of 30-150nm, the protein purity reaches 95% or above, the recovery rate exceeds 85%, and the separation efficiency and quality of the exosome are remarkably improved.

The invention discloses a tumor cell detection kit, and relates to the technical field of detection kits, the tumor cell detection kit comprises a detection uniformizing module, the detection uniformizing module comprises: a first gear rotatably arranged around a first axis of the first gear; the driving disc is in coaxial running fit with the first gear; the gear assembly further comprises at least one set of strip-shaped holes and arc-shaped holes which are matched with each other, the strip-shaped holes are formed in the radial direction of the first gear, the strip-shaped holes are in guide fit with movable assemblies, the movable assemblies comprise matching shafts matched with the arc-shaped side faces of the arc-shaped holes, and the strip-shaped holes and the arc-shaped holes are formed in the first gear and the driving disc respectively. According to the uniform detection module, through the matching mode of the driving disc and the first gear, the overall structural stability is high, the working requirement of low-speed centrifugation can be met, the working requirement of cell debris separation can be met, and the working efficiency is improved. The adaptability of the detection kit is improved.

The invention relates to a battery monomer, a battery and a power utilization device. Each single battery comprises a battery cell and a first insulating part, and the first insulating part wraps the battery cell; the first insulating part is provided with an inner side surface facing the battery cell and an outer side surface deviating from the battery cell, and a first through hole is formed in the first insulating part; the first through hole penetrates through the inner side surface and the outer side surface, and the cross-sectional area of the first through hole on the inner side surface is smaller than that of the first through hole on the outer side surface; alternatively, the first through hole comprises a plurality of first through holes, and each first through hole penetrates through the inner side face and the outer side face. According to the battery monomer, the flow area of the electrolyte can be increased, the flowability of the electrolyte is better, electrolyte infiltration is facilitated, meanwhile, cell falling objects or other foreign matters can be blocked, the condition of metal filing falling or carbon powder falling is improved, the safety is improved, and the service life is prolonged.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

The utility model discloses a stem cell conditioned medium filter device which comprises a filter device shell, a filter tank is arranged in the filter device shell, a square sleeve is arranged on the lower side in the filter tank, a coarse-mesh filter screen is fixedly arranged in the square sleeve, and a filter screen is arranged in the coarse-mesh filter screen. A plurality of fine-hole filter screens fixed in the square sleeve are fixedly arranged below the coarse-hole filter screen; a sealing plate fixed in the square sleeve is arranged above the coarse-mesh filter screen, and a first stepping motor is fixedly mounted in the sealing plate. According to the utility model, the situation that the filter holes in the filter screen are blocked by cell debris, dead cells or metabolic wastes in the stem cell conditioned culture medium and even penetrate through the filter holes due to relatively intense impact generated between the filter screen and the stem cell conditioned culture medium when the filter screen is used for filtering the stem cell conditioned culture medium is prevented; through the technical scheme, the filtering effect of the equipment is improved.

The application relates to a method for purifying umbilical cord blood mesenchymal stem cell-derived exosomes, and relates to the technical field of bioengineering. The method combines gradient centrifugation and membrane filtration. First, the cell culture supernatant is pretreated by adjusting the pH, and cell debris and large-particle impurities are removed by multi-stage differential centrifugation. Then, selective protein precipitation is carried out by using polyethylene glycol with a specific molecular weight, and the exosomes are finely separated by sucrose-meglumine diatrizoate density gradient centrifugation. Finally, terminal purification is completed under low-temperature and low-pressure conditions by using a polyether sulfone membrane filtration system with optimized pore size. By precisely controlling the centrifugation parameters, reagent concentration and filtration conditions, the exosomes obtained by the method have a particle size concentrated in the range of 30-150 nm, a protein purity of more than 95%, and a recovery rate of more than 85%, and the separation efficiency and quality of the exosomes are significantly improved.

This invention provides an in vivo circulating tumor cell (CTC) clearance system, relating to the field of medical device technology. The in vivo CTC clearance system provided in this invention utilizes switching between three pathways: a CTC separation pathway, a CTC electroporation pathway, and a cell debris removal pathway. Based on a metal filter membrane, it continuously separates CTCs in vivo and periodically performs electroporation to break down and remove the collected CTCs. This avoids clogging of the filter pores due to continuous metal filter operation, allowing for continuous, periodic operation to clear all existing and newly generated circulating tumor cells (CTCs) in the body.

The invention relates to a method for operating a biological treatment plant (1) comprising a purification device (2) for removing cell debris from a cell culture fluid (10), in which the purification device (2) comprises a fluidized bed centrifuge (8) having at least one centrifugal chamber (11), in which the cell culture fluid (10) is separated into at least a waste fraction (12) and an output fraction (13), wherein the separation of the cell culture fluid (10) into a waste fraction (12) and an output fraction (13) comprises: a loading step (14) during which the centrifugal chamber (11) is loaded with cells of the cell culture fluid (10) up to a capacity lower than or equal to the maximum cell loading capacity of the centrifugal chamber (11); an overloading step (15) during which the centrifugation chamber (11) is further loaded with a cell culture fluid (10), whereby cells are flushed out of the centrifugation chamber (11); collecting the cells flushed out during the overload step (15) as the waste fraction (12); and collecting cells from the chamber as the output fraction (13).

The invention provides an engineered small extracellular vesicle as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, removing cell debris in a sample to obtain supernate; extracting from the supernate to obtain the small extracellular vesicles; s2, preparing a phospholipid solution; dissolving the polypeptide A with Tris. HCl, and then adding the phospholipid solution for reaction to obtain a coupled polypeptide A system; taking EDC and sulfo-NHS, then adding rhodamine polypeptide B and the small extracellular vesicles, and dissolving the rhodamine polypeptide B and the small extracellular vesicles in tris (hydroxymethyl) aminomethane hydrochloride for reaction to obtain a coupled rhodamine polypeptide B system; and S3, uniformly mixing the coupled FITC polypeptide A system and the coupled rhodamine polypeptide B system, and performing dialysis to obtain the engineered small extracellular vesicles. The obtained engineered small extracellular vesicles are stable, can be supplied continuously, can reduce neurotoxicity effect and relieve the condition of Parkinson's disease, and provide a new target and a new way for clinical treatment of Parkinson's disease.

The invention provides an acellular matrix for treating stress urinary incontinence as well as a preparation method and application of the acellular matrix. Splitting the soft tissue by using a cell lysis solution to remove cell debris; removing nucleic acid, degreasing and then micronizing; and adding the soft tissue particles and the soft tissue cross-linked particles into a phosphate buffer solution according to a ratio, mixing and emulsifying to obtain the acellular matrix for injection for treating stress urinary incontinence. The cell removal rate reaches 99%, the fat residue is less than 0.3%, and the injection is injected around the sphincter of the urethra (middle section or bladder neck) and / or in the urethra according to 0.5 ml / point and 3-4 points during clinical application to play roles in filling, connecting and repairing tissues, so that the damaged urethra supporting structure is repaired, the structural integrity and strength of connective tissues and the sphincter around the urethra are maintained, and the application prospect is wide. Therefore, the symptom of stress urinary incontinence is improved.

A particulate lyophilized platelet lysate composition suitable for use as a cell culture medium can include growth factors, cytokines, and chemokines released from lysis of source platelets, wherein cellular debris from the source platelets is removed (partially or fully) by filtration. The growth factors, cytokines, and chemokines are lyophilized to form a particulate lyophilized platelet lysate composition.

This utility model discloses a vaccine microcarrier recovery filter with a clean structure, including a workbench. A cleaning tank is located at the top of the workbench, and a first water tap is located at one end of the cleaning tank. A water storage tank is located at the bottom of the workbench. A filter groove is formed on the inner wall of the workbench, and a filter is movably installed in the filter groove. This utility model achieves automatic rotational cleaning of the filter through the cooperation of a linear motor and a waterproof motor. This dynamic cleaning method is more efficient than static cleaning, and can more thoroughly remove cell debris, culture medium residues, and microbial contamination from the filter surface, ensuring the cleanliness and effectiveness of the filter.

The invention discloses an LSPR (Local Surface Plasmon Resonance) micro-fluidic chip system for rapid typing detection of various virus antigens, and particularly relates to the cross technical field of biomedical engineering, a micro-nano sensing technology, molecular diagnosis and micro-fluidic chip application. According to the present invention, by introducing the PEGylation anti-fouling layer and the BSA sealing treatment, the non-specific adsorption interference is effectively reduced, and compared with the problems that the traditional antigen rapid detection (Ag-RDT) is easily influenced by the virus epitope mutation and the variation is difficult to distinguish, the system can utilize the LSPR peak position red shift difference caused by the combination of different variation antigens and the specific antibody to achieve the precise typing of the multi-variant virus, meanwhile, white light dark field hyperspectral detection is combined with baseline correction, noise filtering and other algorithms, spectral signals can be accurately extracted, and even if interference components such as impurity protein and cell debris exist in a sample, feature signals of a target antigen can still be stably recognized.

The invention belongs to the technical field of biological medicine and clinical medicine, and particularly relates to an efficient purification method of brain tissue small extracellular vesicles. The purification method comprises the following steps: cutting mouse brain tissues into uniform small blocks by using a steel wire mesh, digesting and dissociating, filtering, taking filtrate, and fixing the volume of the filtrate by using an EBSS buffer solution to obtain a tissue supernatant; the method comprises the following steps: sequentially removing complete cells, cell debris, plasma membranes and large vesicles through gradient centrifugation, filtering, taking filtrate, carrying out ultracentrifugation to obtain sEVs precipitates, and resuspending the sEVs precipitates with a PBS buffer solution to obtain a sEVs crude purification product; and preparing a single sucrose pad from a sucrose solution with the concentration of 25-35% and a PBS buffer solution, and carrying out ultracentrifugation on the sEVs crude purification product, wherein the obtained precipitate is the purified brain tissue sEVs. The purification method provided by the invention solves the problems of tedious flow, long consumed time, low yield, poor purity and the like in the existing sEVs purification technology.

The invention discloses an integrated micro-fluidic metasurface chip for real-time detection of exosome (POCT). The chip is formed by bonding a micro-fluidic structure layer and an optical sensing substrate layer, and sequentially comprises a sample / buffer solution converging region, a gradient film pretreatment region, a snake-shaped mixing region, a micro-column enrichment region and a multi-channel super-surface detection region in the flow direction. The coarse filtration membrane and the fine filtration membrane realize graded removal of cell debris and platelets, and are matched with a back-flushing structure to reduce blockage of the filter membranes; the microcolumn array is modified by a general exosome antibody and is used for efficient immune enrichment and mild washing or elution of exosome; the multi-channel metasurface array is used for respectively fixing different disease-related probes and carrying out high-sensitivity parallel optical detection on multiple markers of exosomes. According to the present invention, the single-chip integration of exosome pretreatment, enrichment and detection is achieved, the sample loss is reduced, the detection sensitivity and the flux are improved, and the method is suitable for the POCT application scenarios such as tumor early screening. Figure 1 is a structural diagram of the integrated micro-fluidic metasurface sensing chip provided by the invention.

The present invention provides a method for obtaining a composition comprising exosome-like vesicles or biomimetic exosomes from microalgae cells comprising a a)homogenizing microalgae cells by high-pressure, and b) eliminating cell debris from the homogenized solution obtained in step a). The invention also relates to a composition obteined by said method and to cosmetical and medical uses thereof.

The invention relates to a method for removing tissue fragments and impurities in a cell suspension, which comprises the following steps: S1, acquiring a cell suspension, centrifuging the cell suspension, removing supernate, and mixing with a cell precipitation resuspension to obtain a cell resuspension; s2, obtaining a gradient separation liquid and a centrifugal tube, adding the gradient separation liquid into the centrifugal tube, and adding the cell resuspension along the inner wall of the centrifugal tube to form a separation liquid layer and a cell suspension layer in the centrifugal tube; s3, carrying out gradient centrifugation on the centrifugal tube, taking the precipitate, and resuspending to obtain a purified cell suspension; wherein the gradient separation liquid comprises a Percoll solution or a sucrose solution. According to the technical scheme, fragments and impurities are separated by using a gradient density centrifugation method, and living cells, cell fragments and impurity fragments with relatively low density can be separated. And part of dead cells break cell membranes in the centrifugation process, become cell fragments and are removed along with density gradient centrifugation.

The application discloses a cell microdroplet identification method based on XGBoost, and relates to the technical field of single-cell RNA sequencing, wherein step one is to search for the boundary of cells and empty droplets by using a RankMSE index and to construct a training set, and to solve the class imbalance problem in the training data by using multiple rounds of downsampling; step two is to combine the gene expression of cells and the cell quality control features calculated in advance such as cell entropy, and to use a machine learning method XGBoost to construct a cell-empty droplet binary classification model suitable for the current data; and step three is to iterate the cell-empty droplet binary classification model, add newly predicted empty droplet data to the model for retraining, and obtain an optimized prediction model. The cell microdroplet identification method based on XGBoost has the advantages that cells can be robustly identified in different data sets, and empty droplets and low-quality cells or cell fragments in the data can be effectively excluded, and the method has higher accuracy and stability compared with the prior art.

The invention relates to the technical field of biological medicine, in particular to a gene recombination cell exosome and application thereof in preparation of anti-cancer drugs. The gene recombinant cell exosome is extracted from an IL15K562 cell culture supernatant by the following method, and the method comprises the following steps: (1) collecting the IL15K562 cell culture supernatant, and centrifuging to remove cell debris; (2) adding a treatment reagent, uniformly mixing, and incubating for 6-20 hours; and (3) centrifuging to obtain a precipitate, further suspending the precipitate in a buffer solution, and centrifuging again to obtain the gene recombinant cell exosome. Researches show that the gene recombinant cell exosome prepared from the IL15K562 cell culture supernatant by the method disclosed by the invention has a remarkable anti-cancer effect; therefore, the compound has important application value when being used as an active ingredient for preparing the anti-cancer drugs.

This invention relates to the field of biotechnology, particularly to the field of IPC G01N1, and further to a method for preparing and applying ultrathin cell sections. The method includes the following steps: (1) sample collection; (2) pre-fixation; (3) agar pre-embedding; (4) fixation; (5) dehydration, permeation, and embedding; (6) ultrathin sectioning; and (7) staining. This invention uses tannic acid instead of osmium tetroxide for sample fixation, avoiding the harm of osmium tetroxide to human health and reducing costs. During tannic acid fixation, uranium acetate is added for further fixation, which avoids incomplete cell membranes, prevents the formation of large amounts of cell debris, and prevents cell swelling, further improving the subsequent staining effect, especially suitable for the fixation of CHO cells.

The invention relates to a method for separating and purifying osteopontin from human milk, which comprises the following steps: (1) pretreatment: collecting human milk and pretreating the human milk to remove fat and cell debris impurities to obtain pretreated emulsion; (2) osteopontin precipitation: adding a precipitator into the pretreated emulsion to precipitate osteopontin, and collecting the precipitate; (3) osteopontin dissolution: adding a solvent capable of dissolving osteopontin into the obtained precipitate, fully mixing, and then removing insoluble substances to obtain an osteopontin crude solution; (4) dialyzing and concentrating: dialyzing and concentrating the osteopontin crude solution to obtain an osteopontin concentrated solution; (5) chromatography: purifying the osteopontin concentrated solution in a chromatography manner to obtain an osteopontin purified solution; and (6) drying: drying the osteopontin purified liquid to obtain osteopontin powder. By adopting the method disclosed by the invention, the osteopontin purity of 95% or more by mass can be efficiently realized.

The present invention relates to a method for obtaining exosome-like vesicles from microalgae. The present invention provides a method for obtaining a composition comprising exosome-like vesicles or biomimetic exosomes from microalgae cells, said method comprising a) homogenizing the microalgae cells by high pressure, and b) removing cell debris from the homogenized solution obtained in step a). The present invention also relates to the composition obtained by said method and to its cosmetic and medical uses.

Methods for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer, based on the presence of certain structures associated with circulating cancer associated macrophage-like cells (CAMLs), including micronuclei (MN), extracellular vesicles (EVs), enlarged polynuclearization (EPN), internalized intact cells and large internal cellular debris, are provided.

The invention discloses a suspension cell continuous culture process and an impurity removal method of a cell suspension, and belongs to the technical field of cell culture. In the continuous culture process of the suspension cells, dead cells, cell agglomerates and / or cell debris are removed by adjusting the standing and settling conditions, especially the temperature, so that damage to the cells caused by centrifugation, introduction of exogenous reagents and the like is avoided, and the high cell viability is maintained. Dead cells, cell agglomerates and / or cell debris in a cell suspension in a reactor are regularly removed in a continuous culture process, so that single cells with high cell activity and high purity are continuously obtained, rapid and high-value multiplication of the cells is realized, and a guarantee is provided for the stability of subsequent experimental research and industrial production.

To provide antioxidant compositions containing cell debris of Thraustochytrids microorganism as an active ingredient, and methods for producing the cell disruption suspension.SOLUTION: The present inventors have found that the cell disruption suspension of Thraustochytrids microorganisms has excellent antioxidant activity, and completed the present invention.SELECTED DRAWING: Figure 1

The present invention provides a method for determining the resistance of a microorganism to a drug by detecting at least a transcript of a drug resistance gene from a microorganism in a biological sample, the method comprising the steps of: (i) lysing the cells by means of a chemical or a mechanical method, thereby obtaining a lysate and cell debris; (ii) obtaining a ribosome-antibody complex from the lysate using an antibody or a fragment thereof which binds specifically to a microorganism-ribosomal protein; (iii) purifying the mRNA associated to the ribosome-antibody complex by means of a nucleic acid extraction method; and (iv) submitting the resulting mRNA to a specific gene detection method, thereby identifying the at least one drug resistance gene transcript of the biological sample. Provided methods and kits allow determining the resistance to antibiotics of a biological sample in a rapid and reliable manner, thereby minimizing the risk of AMR and allowing the definition of the therapeutic potential of a selected antibiotic