The invention discloses a method and a device for observing the conformation transition of macromolecules and the biochemical reaction between target molecules and probe molecules. The method comprises the steps as follows: the macromolecules and one or more of the probe molecules which are different or identical are arranged on single side surfaces of various micro-cantilevers in a micro-
cantilever array; the incident parallel rays emitted from a
light source reach the micro-cantilevers, and the reflection rays are received by an optical
receiver assembly to form an array of reflection
ray spots corresponding to the micro-cantilevers one by one; when the conformation transition of macromolecules occurs or the combination of the probe molecules and the target molecules present in a solution to be tested occurs, the micro-cantilevers bend and deform with the deflection of the reflection rays, and accordingly, the positions of the corresponding reflection
ray spots change; and according to the position change and the displacement of the reflection
ray spots corresponding to each micro-
cantilever, the conformation transition of the macromolecules can be learned, whether the target molecules corresponding to the probe molecules are present or absent in the solution to be tested can be determined, and the concentration of the target molecules present in the solution can be obtained. The invention can realize the simultaneous observation of the displacement signals of a plurality of micro-cantilevers, and at the same time eliminate the
noise signals due to the variation of the
refraction index and the change of the intensity of a
light source. The invention has the advantages that the effects of vagabond rays can be greatly reduced by controlling the measurement of the
light beam polarization state, and the
measurement precision is improved.