21 results about "Diafiltration" patented technology

The present disclosure provides high viscosity ultrafiltration / diafiltration (UF / DF) and single pass tangential flow filtration (SPTFF) processes for the purification of polypeptides. A method for purifying a polypeptide from a polypeptide preparation can include the following steps in sequence: a) subjecting the polypeptide preparation to one or more purification processes and recovering a first resulting pool having a viscosity of about 5 to 40 cP; and b) subjecting the pool recovered from step a) to an SPTFF operation and recovering a second resulting pool having an operating viscosity of about 41 to 400 cP, the SPTFF operation comprising the use of membranes in either or both of a tandem membrane configuration and / or a parallel membrane configuration, where i) the operating temperature is about 15 to 55 DEG C, and ii) the feed flux is about 5 to 50 L / m2 / hr (LMH).

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange / hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration / diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

The present disclosure provides methods for deprotecting and purifying an oligomeric compound. The method provides for deprotection of a protecting group, which may be a trityl protecting group, and removal of a byproduct by diafiltration. Also provided are oligomeric compounds prepared by the method.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and / or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and / or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg / g; and a heavy metal content of less than about 60 μg / g.

Systems, devices, and methods for managing air gaps in a semiconductor device are provided. In one aspect, a method includes removing coelomic fluid from an exterior surface of the worms using a drying agent; removing blood from the worms; performing centrifugation on the blood to generate a blood supernatant; concentrating, by a tangential flow filtration system with a single membrane, the blood supernatant to form a concentrated blood product; and performing, by the tangential flow filtration system, tangential flow filtration of a diafiltration mixture comprising the concentrated blood product.

Disclosed herein is a purification process comprising an organic ultrafiltration / diafiltration process in which a crude mixture containing a conjugate compound to be purified is filtered through a semipermeable membrane and the filtrate from the crude mixture is replaced with an exchange medium containing an organic solvent and a buffer system.

The invention is directed to a composition of soluble placental ECM in storage solution and the methods of manufacture related thereto. The solubilized placental ECM solution is manufactured through a process of partitioning placental ECM from the placental tissue in a high acidic pH environment and performing an ultrafiltration step and diafiltration step through a tangential flow filtration system to increase the pH. The soluble placental ECM retains the native characteristics of placental ECM components and includes collagens (types I, III, V, VI, IX, and X), elastin, laminin, and fibronectin.

A method for improving the harvest or purification of a target protein such as a biologic or biosimilar is provided. The method improves conventional harvest / purification methodologies by adding a chromatography step, such as a mixed-mode or ion exchange chromatography step, towards the end of the polishing phase of harvest / purification, after conventional chromatographic polishing steps such as protein A or ion exchange chromatography steps have been completed and the resultant eluate subjected to filtration, such as ultrafiltration / diafiltration. The surprising result of returning to chromatographic polishing after filtration is that all forms of high molecular weight products are reduced, facilitating the purification of target protein sufficient to meet government regulations, such as Quality Target Protein Profiles.

Disclosed is a high resolution affinity chromatography for analyzing a capture process and an affinity capture process using a single chromatography matrix combination, the high resolution affinity chromatography resulting in an improved resolution between closely related molecular species, and the total product yield of large-scale commercial production of heterodimer proteins (such as bispecific antibodies) is significantly improved. Furthermore, storage tank and equipment requirements are reduced by reducing salt concentration while increasing product purity and concentration without dilution, ultrafiltration or percolation capability.

High resolution affinity chromatography combining affinity resolving and affinity capture processes using a single chromatography matrix results in improved resolution between closely related molecular species and significantly enhances overall product yield for large scale commercial production of heterodimeric proteins such as bispecific antibodies. Moreover, tankage and equipment requirements are reduced via the ability to reduce salt concentration, while increasing product purity and concentration, without the need for dilution, ultrafiltration or diafiltration.

The present invention describes a novel process for the isolation of glycosphingolipids lacking amide-linked fatty acyl groups, comprising the steps of providing a solution comprising the glycosphingolipids of formula (1) or salts thereof and one or more contaminants, percolating the solution by using a membrane having an MWCO of 100-300 kDa, separating the percolated glycosphingolipids from the percolated glycosphingolipids, and separating the percolated glycosphingolipids from the percolated glycosphingolipids. And separating the glycosphingolipids or salts thereof from one or more contaminants, thereby obtaining a diafiltration trapped fluid (DFR) comprising the glycosphingolipids or salts thereof, thereby separating the glycosphingolipids or salts thereof from the one or more contaminants.

The present invention describes a method for producing an undenatured pre-acidified whey protein isolate and a protein-fortified beverage incorporating such an undenatured pre-acidified whey protein isolate. The method comprises the steps of ultrafiltration of a liquid whey composition to obtain a liquid whey protein-retaining solution and a liquid whey permeate, and microfiltration of the liquid whey protein-retaining solution to obtain a microfiltered whey protein-retaining solution and a microfiltered whey protein permeate. The method comprises the steps of lowering the pH of the microfiltered whey protein permeate to less than 3.8 to form a pH-reduced whey protein permeate, and ultrafiltration and diafiltration of the pH-reduced whey protein permeate to obtain the undenatured pre-acidified whey protein isolate. The method optionally comprises the step of nanofiltration of the undenatured pre-acidified whey protein isolate.

The present invention in particular relates to a method of producing an immunogenic composition exhibiting reduced virucidal activity, as well as to the immunogenic composition and uses thereof, wherein the method in particular comprises the steps of: (a) providing a mixture with a first liquid and a recombinant protein, (b) concentrating the recombinant protein in the mixture by removing a portion of the first liquid from the mixture, and (c) processing the solution resulting from step (b) by continuous diafiltration.

The invention relates to the medical and food industry. What is proposed is a method for producing a composition of metallic micronutrient chelate complexes. According to the invention, a 5-20% aqueous solution of micellar casein has added thereto a solution of a chloride of one or more metallic micronutrients with a concentration of 40-50%, wherein the ratio of the mass of the protein to the mass of the salt is 5:1 - 3:1. The mixture is then held for 40-120 minutes at a temperature of 30-60°С and a рН of 7.5-9.4 under agitation, with the given pH value being continuously controlled and maintained by titration. The resulting aqueous micellar casein chelate solution is then purified of residual inorganic salts by microfiltration and diafiltration with sublimation or spray drying. The dried micellar casein-based chelates are mixed, in an amount that satisfies the daily micronutrient requirement, with a pharmaceutically acceptable carrier. The following are used as metallic micronutrients: calcium, magnesium, zinc, copper, iron, manganese, chromium and cobalt. The concentration of micronutrients in the resulting composition is 0.01-100 mg of micronutrients per gram of the composition. Not less than 90% of the micronutrients are chemically bound to the micellar casein. The invention is directed toward increasing the effectiveness of a composition of metallic micronutrient chelate complexes by virtue of particle size uniformity.

The present invention relates to a process of purifying a protein. Specifically, the present invention relates to a process of purification of a polyclonal antibody and / or antibody fragments. The present invention employs an adsorptive separation using anion exchange chromatography and cation exchange chromatography along with ultrafiltration and diafiltration to achieve high-purity antibody / or antibody fragments. The process provided in the present invention is applicable for polyclonal antibody and / or antibody fragments, which are associated with conditions but not limited to snake envenomation, scorpion envenomation, diphtheria infection, tetanus infection, rabies virus infection and gas gangrene infection due to Clostridium species.

The present disclosure provides for a preparation method of highly concentrated antibody solution that binds to human programmed death receptor 1 (PD1). This process is able to manufacture the high concentrated antibody solution by an ultrafiltration / diafiltration (UF / DF) unit operation described herein. The UF / DF preparation method comprises a single-pass tangential flow filtration (SPTFF) as the first ultrafiltration concentration step, a buffer solution diafiltration step and a second ultrafiltration concentration step using conventional UF / DF. The process has a broad operation parameter range and maintains antibody stability and integrity.

This invention provides a method for stabilizing proteins in aqueous solutions during protein purification and / or concentration. [Solution] (a) Protein and general formula (I): JPEG2026062713000015.jpg46170 The method comprises the steps of: (a) providing an aqueous solution containing a polyalkoxy fatty acid acyl surfactant; (b) contacting the aqueous solution with a separation membrane; and (c) subjecting the aqueous solution to a diafiltration step and / or an ultrafiltration step to produce a retention solution product which is an aqueous solution containing protein, wherein the compound of formula (I) reduces protein aggregation in steps (a) to (c) of the method, and the compound of formula (I) passes through the separation membrane in step (c).

High resolution affinity chromatography combining affinity resolving and affinity capture processes using a single chromatography matrix results in improved resolution between closely related molecular species and significantly enhances overall product yield for large scale commercial production of heterodimeric proteins such as bispecific antibodies. Moreover, tankage and equipment requirements are reduced via the ability to reduce salt concentration, while increasing product purity and concentration, without the need for dilution, ultrafiltration or diafiltration.

Disclosed herein are purification methods including an organic ultrafiltration / diafiltration process wherein a crude mixture containing a conjugate compound to be purified is filtered through a semi-permeable membrane, and filtrate from the crude mixture is replaced with an exchange medium containing an organic solvent and a buffer system.

The application discloses a medical collagen extraction method and belongs to the technical field of biomedical materials. The extraction steps are as follows: after removing surface foreign matters from a bovine Achilles tendon, the bovine Achilles tendon is cut into particles, washed by a NaCl solution and soaked by a Tris-HCl buffer solution containing EDTA-Na2; the pretreated bovine Achilles tendon tissue is added into a swelling treatment liquid prepared by taking lactic acid and betaine as raw materials to perform soaking, so that swelled bovine Achilles tendon tissue is obtained; the swelled bovine Achilles tendon tissue is subjected to enzymolysis by pepsin, so that a crude collagen extraction liquid is obtained; poly(N-isopropyl acrylamide) is added into the crude collagen extraction liquid to precipitate a collagen-containing precipitate; after resuspension of the precipitate, constant-volume ultrafiltration diafiltration purification is performed, so that a collagen concentrate liquid is obtained, and the medical collagen extraction is completed. The product has the characteristics of high purity, structural integrity and low endotoxin, meets medical-grade safety standards and is suitable for large-scale popularization and use.

Methods and systems for real-time monitoring and control of an ultrafiltration / diafiltration (UF / DF) conditioned pool in a UF / DF collection tank are disclosed. In various embodiments, the UF / DF pool is received in the collection tank for conditioning or dilution with a buffer. In-line Raman measurements of the conditioned UF / DF pool can be performed and provided as input to a trained machine learning model. The machine learning model can then predict product quality attributes of the UF / DF conditioned pool, such as the protein concentration and osmolality of the conditioned UF / DF pool.