467 results about "Phloroglucinol" patented technology

The present invention provides methods, enzymes, and cells for the biosynthetic production of phloroglucinol from malonyl-CoA, which is ultimately obtained from simple starting materials such as glucose; also provided are methods for preparing derivatives of biosynthetic phloroglucinol, including, e.g., resorcinol.

The present invention relates to the preparation process of phloroglucinol superior to available preparation process. The present invention features that the preparation process of phloroglucinol includes the complexing reaction between anhydrous aluminum trichloride as complaxing agent and 1, 3, 5-trimethoxy benzene, hydrolysis in concentrated hydrochloric acid solution, cooling and crystallization of hydrolysate to obtain coarse phloroglucinol product, decolorizing with active carbon and re-crystallization in water to obtain refined phloroglucinol product. The said preparation process of the present invention has phloroglucinol product with good color and high purity, short reaction time, less waste liquid exhaust, simple operation condition and high reaction stability.

The invention provides a method of treating the phloroglucinol producing wastewater and the recycle utilization of the recovery organic compounds. The wastewater of the phloroglucinol producing with 2, 6-dichlorophenol and excess KOH as material is adjusted by hydrochloric acid to pH less than 3 before adsorbed by high-crosslinked adsorption resin. The secondary effluent water of adsorption is adjusted to be neutral by KOH before distilled. The KCl is recovered after the cooling crystallization and the centrifugal of the residue. The distilled effluent returns to the former manufacturing process to be indiscriminate used or biochemical treated by aerobic and discharges in standard. The distilled mother liquor merges into other batch of secondary effluent water of adsorption. The resin adsorbing the phloroglucinol is desorbed by KOH. The desorption liquid is applied in the hydrochloric acid acidization step of the former manufacturing process or hydrochloric acid is adopted directly to neutralize to be neutral before centrifugal separation to get phloroglucinol. By adopting the method, phloroglucinol and KCl can be recovered while the wastewater is treated. The pollution is reduced and the cost is saved. The organic combination of wastewater treatment and resource recovery as well as recycle utilization is realized.

The invention discloses a method for improving phloroglucinol synthetic capability of recombinant cells, and particularly relates to a method for improving the yield of phloroglucinol synthesized by a biological method through recombinant cell reconstruction by metabolic engineering means.

The invention discloses a preparation method of a covalent organic framework/carbon nitride composite material and application thereof. Firstly, a covalent organic framework material is synthesized byusing p-phenylenediamine and triformyl phloroglucinol as synthetic monomers through a room-temperature solid-phase method, then the covalent organic framework material and carbon nitride are mixed, stirred and dried in methyl alcohol, and pyrolysis is carried out under the protection of inert gas to obtain a target product. The covalent organic framework/carbon nitride composite material disclosed by the invention is large in specific surface area, high in nitrogen doping amount and rich in pore structure and can effectively remove toxic organic pollutants in water by constructing a novel Fenton-like system, and new application of the covalent organic framework material in the field of catalysis is expanded; unavoidable metal ion leaching in the activating process of a metal nano catalystis overcome; and the preparation method has the advantages of simple process, easiness for repeated operation, controllable structure, suitability for large-scale production and the like.

The invention relates to a composite material of core/shell structure having covalent organic framework, wherein the composite material is formed by coating a core, formed from a magnetic particle, with a shell, formed from the covalent organic framework material, a core having a structure of Fe3O4 particles coated with a polydopamine layer, wherein the particle is modified by amino-containing alkoxysilane so that -NH2 functional group is formed on the surface of the polydopamine layer; a shell structure being a reaction product of 3,3'-dimethoxybenzidine and trialdehyde phloroglucinol. The invention further relates to a preparation method and an application of the composite material.

The invention provides a preparation method for carbon aerogel for an electric adsorption electrode. The metal nickel modified carbon aerogel is prepared by performing sol-gel reaction, normal-pressure drying and high-temperature carbonization on resorcinol, phloroglucinol, nickel acetate and formaldehyde which serve as raw materials and sodium carbonate serving as a catalyst. According to the preparation method, the reaction speed is properly adjusted by adjusting the content of the phloroglucino in the raw materials; by virtue of solvent replacement, organic aerosol is prepared under normal-temperature and normal-pressure conditions. The method provided by the invention is used for preparing the nickel-loading carbon aerogel, so that the specific surface area of the carbon aerogel is large, the pore volume and the aperture are large, and the resistivity is low; the carbon aerogel can be widely applied in the fields of treatment of a supercapacitor electrode and organic wastewater, removal of negative and positive ions, heavy metal ions and radioactive isotopes and the like. The preparation method provided by the invention is simple in preparation process and convenient and easy to operate and is short in production period; requirement on equipment is low, and industrialization development and popularization application are facilitated.

The invention discloses preparation and application of a covalent organic framework porous nano composite material, and belongs to the technical field of environmental protection. Firstly, 2,4,6-trialdehyde phloroglucinol (Tp) and benzidine (BD) are subjected to a Schiff base reaction under proper conditions to prepare a covalent organic framework (COF-TpBD), then diindium trisulfide (In2S3) nanoparticles are loaded on the COF-TpBD in situ, and the composite material (In2S3@COF) with In2S3 and COF characteristics is obtained. The In2S3@COF prepared by the method is rich in a large amount of Selement and can adsorb Hg2+ through HgS interaction, and on the other hand, the inherent large specific surface area and excellent pore size of the COF can also greatly improve the adsorption capacityto Hg2+. Inductively coupled plasma mass spectrometry test results show that the In2S3@COF has excellent Hg2+ adsorption performance. The method is simple, stable in structure, low in cost, environmentally friendly and high in adsorption and removal efficiency of Hg 2+ in a water body, and can be used as an efficient adsorbent for Hg 2+ in environmental wastewater.

jA process for preparing phloroglucinol from 1,3,5-trimethoxy benzene includes reacting on lewis acid in the hydrocarbon-type solvent to generate intermediate, acidic hydrolyzing by inorganic acid, layering, crystallizing in liquid layer, and recrystallizing. Its advantages are short time, high productivity and high purity and output rate of product.

A method for preparing carbon aerogels and carbon aerogels obtained therefrom are disclosed. The method for preparing carbon aerogels comprises: mixing organic starting materials including phloroglucinol and furfural with a solvent capable of dissolving the organic materials in a predetermined ratio to form a sol solution; adjusting pH of the sol solution adequately by using an acidic or basic catalyst, gelling the sol solution at room temperature under atmospheric pressure, and aging the resultant gels; substituting the solvent in thus obtained gels with liquid carbon dioxide, followed by drying in a supercritical state, to form organic aerogels; and pyrolyzing the organic aerogels in an electric furnace under inert atmosphere to obtain carbon aerogels. Particularly, the gels are formed at room temperature in a short period of time by adequately adjusting pH of the sol solution. Therefore, the method provides improved time efficiency and energy efficiency as compared to existing methods for preparing gels. Additionally, the method allows supercritical drying while avoiding a need for an additional solvent substitution, thereby simplifying the overall process. Further, the method enables preparation of carbon aerogels for supercapacitors having a high specific surface area and high capacitance even in the absence of additional activation step.

A low molecular weight chondroitin sulfate preparation method relates to chondroitin sulfate. The method includes the steps of stewing sturgeon to remove flesh and fat on the surface of bone, soaking with petroleum ether, washing with ethanol, drying and pulverizing to obtain cartilage particles; adding a NaOH solution to the cartilage particles, stirring for the first time and adjusting the pH to neutral with acid to obtain a cartilage mother solution; adding malic acid to the cartilage mother solution, adjusting the pH to 3.0 to 3.2, adding pepsin, stirring for the second time, adjusting the pH to 5.0 to 5.5, adding papain and cellulase, stirring for the third time, adding activated carbon, stirring for the fourth time and filtering to obtain clarified enzymatic hydrolysate; subjecting the enzymatic hydrolysate to resin adsorption, gradient elution in a NaCl solution, phloroglucinol spectrophotometry detection and combined collection to obtain an eluent with color reaction; precipitating the eluate with ethanol, collecting precipitate through centrifugation and drying to obtain low molecular weight chondroitin sulfate crystals.

The invention discloses a cleaning agent suitable for cleaning thick photoresist and with low etching performance, which contains (a) potassium hydroxide, (b) dissolvent, (c) pentaerythritol, (d) alkylol amine and (e) phloroglucinol. The cleaning agent for the photoresist can also be used for removing the photoresist and other residues on metal, metal alloy or dielectric medium substrates, has low etch rate on metal including copper (Cu) and the like simultaneously, and has good application prospect in microelectronics fields including semiconductor chip cleaning and the like.

The invention discloses a genetic engineering bacterium for high-yield phloroglucinol as well as a construction method and an application of the genetic engineering bacterium and belongs to the technical field of genetic engineering. The genetic engineering bacterium over-expresses carbon storage and regulation factor gene CsrB shown by SEQ ID NO.1 and co-expresses a polyketide synthase gene phlD, a multiple resistance activating factor gene marA and acetyl CoA carboxylase gene ACCase. The invention also provides the construction method and the application of the genetic engineering bacterium for the high-yield phloroglucinol. With the adoption of the genetic engineering bacterium, the yield of the phloroglucinol is firstly increased by 115.6 percent by adopting a mode of globally regulating carbon metabolism after post-transcriptional level of the genetic engineering bacterium, and the genetic engineering bacterium has a high industrial application value.

The invention belongs to the field of medicinal preparations, and discloses a method for preparing phloroglucinol injection. The method for preparing the phloroglucinol injection comprises the following steps of: (1) preparing solution containing phloroglucinol and trimethyl phloroglucinol; (2) stirring the solution uniformly and then filtering the solution for sterilization; (3) encapsulating the filtered solution by a conventional method and sterilizing the encapsulated solution, wherein the temperature for the sterilizing filtration in the step (2) is 5 to 40 DEG C, and preferably 10 to 35 DEG C. The technical scheme can ensure stable content of the trimethyl phloroglucinol during the preparation of the phloroglucinol injection. Meanwhile, the method for preparing the phloroglucinol injection ensures the sterility assurance level of the injection by using the sterilization process with over sterilization effect.

An aqueous adhesive composition includes: a phenol/aldehyde resin; and an unsaturated elastomer latex. The phenol/aldehyde resin is based on at least: an aromatic polyaldehyde bearing at least two aldehyde functional groups and including at least one aromatic nucleus; and phloroglucinol. The adhesive composition is useable to make a textile material adhere to a crosslinkable rubber composition, and may be used as an advantageous alternative to a formaldehyde-based RFL adhesive.

The invention discloses a phloroglucinol derivative capable of inhibiting PTP1B activity, and a preparation method and applications thereof, and more specifically relates to a phloroglucinol compound which is obtained via extraction and separation from Eugenia jambolana Lam seeds, a preparation method thereof, and applications of the phloroglucinol compound in preparing drugs used for treating diabetes and obesity, or inhibitors of protein tyrosine phosphatase 1B (PTP1B). The general molecular formula of the phloroglucinol compound is represented by formula I, wherein R1 is used for representing methyl or hydrogen, R2 is used for representing methyl or hydrogen, R1 and R2 are used for representing the same or the different groups, and R3 is used for representing an alkane or olefin side chain containing 12 to 26 carbon atoms. The phloroglucinol compound is a novel compound with excellent PTP1B inhibition effect; the PTP1B inhibition activity IC50 value ranges from 0.42 to 3.16<mu>M; the phloroglucinol compound can be used for preparing PTP1B inhibitors, and drugs used for treating diabetes, obesity, and complications of diabetes and obesity.

The invention relates to a method for synthesizing flavone compounds, in particular to a method for synthesizing 5,7-dihydroxyflavone. The method comprises the following steps: in the presence of a condensing agent, reacting an active methylene compound (ethyl acetoacetate) with an acylating agent (benzoyl chloride), and importing benzoyl into the active methylene; selectively hydrolyzing the obtained product by utilizing ammonium chloride solution so as to remove acetyl, thus obtaining ethyl benzoylacetate; mixing the obtained intermediate with phloroglucinol to dissolve the obtained intermediate, then warming, vacuumizing and reacting to obtain a crude product; and refining the crude product by utilizing DMF (dimethylformamide) and ethanol so as to obtain the 5,7-dihydroxyflavone. The method provided by the invention has the advantages of rich sources of feed, low cost and short reaction step, and is simple in operation; and meanwhile, through the optimization of reaction conditions, the method also has the advantages of short reaction step, high yield, low cost, less environmental pollution and the like. The products prepared by the method can be used for preparing medicaments and the like.

The present invention relates to an improved preparation method for synthesizing phloroglucin. Said invention uses 2.4-dichlorophenol or 2.6-dichlorophenol as original raw material, and utilizes potassium hydroxide and inert solvent with high boiling point to make reaction of first-elimination after-addition to prepare phloroglucin by means of one-step process. Its yield is 56.6%-65.2%.

The invention discloses a preparation method for phloroglucinol, comprising the following steps: mixing 1, 3, 5-trimethoxy benzene and concentrated hydrochloric acid or concentrated sulfuric acid, and adding H3PO4 into them, and stirring them for 24-48 h at room temperature; adding sodium carbonate into them in batches and neutralizing them, and regulating the pH value to 2-3 and filtering them; extracting the filter liquid by ether in batches, and drying the extraction liquid and evaporating out ether to obtain yellow solid phloroglucinol. The preparation method has an advantage of low production cost. The yield of the obtained phloroglucinol is higher than 80% and purity thereof is higher than 90%.

A method for synthesizing phloroglucinol by microbial catalysis relates to a method for synthesizing phloroglucinol by catalysis of engineering escherichia coli cells. The method for synthesizing phloroglucinol by catalysis of engineering escherichia coli cells comprises the following steps of: compounding, inducing, selecting and optimizing anti-phloroglucinol mutant strains by employing HNO2 and LiCl; carrying out PCR amplification by taking the genome DNA of bacterium Pseudomonas fluorescens Pf-5 as a template to obtain a polyketide synthase gene (phlD); connecting with a pET carrier after cleavage, transferring to the anti-phloroglucinol mutant strains, and inudcing and expressing a target protein by using IPTG to obtain the engineering escherichia coli for catalyzing and synthesizing the phloroglucinol. The phloroglucinol can be obtained in the fermentation liquor after the engineering escherichia coli are fermented, and the phloroglucinol is extracted and concentrated to get the finished phloroglucinol. The invention first provides the method for synthesizing phloroglucinol by catalysis of the engineering escherichia coli, and lays a solid foundation for catalyzing and synthesizing phloroglucinol by an environment-friendly synthetic process in future.

The invention relates to a method for obtaining antioxidant extracts from macroalgae using ultrasound-assisted continuous aqueous extraction. The process can be performed using fresh algae or dry algae, after resuspending same in water. A suspension of alga in water is prepared with a solid concentration of 10% to 30%. The mixture is fed to an ultrasonic breakdown system. The extract is filtered and lyophilised, obtaining total polyphenol concentrations of 62.4 mg eq. of phloroglucinol /g of lyophilizate with Bifurcaria bifurcata and 44 mg eq. of phloroglucinol /g of lyophilizate with Ascophyllum nodosum. The extract can be used as an ingredient in cosmetic and food formulations.

The invention discloses a phloroglucinol orally disintegrating tablet and a preparation method thereof. The phloroglucinol orally disintegrating tablet is prepared by adopting a powder pelletization and compression method by a wet method and contains a therapeutically effective amount of phloroglucinol and a pharmaceutical excipient, and the excipient comprises a disintegrant; and according to the total weight of the tablet, the content of the phloroglucinol is between 15 and 30 percent, the content of the excipient is between 70 and 85 percent, and the content of the disintegrant is between 3 and 23 percent. The phloroglucinol orally disintegrating tablet has the advantages of higher hardness, simple preparation technology, and remarkable curative effect, is quickly disintegrated within 10 seconds at the soonest when contacting saliva in an oral cavity, and is convenient for patients to take.

The sodium hydroxide solution of phloroglucinol is as the detection liquid. The concentration of phloroglucinol is 1% and the concentration of sodium hydroxide is 12%. Several portions of formalin with the concentration from 0-500 ppm are utilized as the standard substances. When detection of formalin ingredient is carried out, the detection liquid drops to the samples to be tested and the several standard substances. With the color being changed, observing the changer color making the qualitative or semi quantitative determination. The color of the sample to be tested is compared with the colors of standard substances with different grads. If the color of the sample tested is between the colors of standard substances with adjacent concentration values, then the formalin concentration of the sample to be tested is between the products from the concentration and the diluent times.

The invention relates to a preparation method of a porous carbon nanosphere with a controllable diameter. In terms of weight, phloroglucinol, terephthalaldehyde and water are taken in the proportion of 1:0.7-0.9:300-340. A phenolic prepolymer solution is obtained after being reacted for 15 minutes to 12 hours at the stirring speed of 400-1000 revolutions per minute at the temperature of 70 DEG C. In terms of weight, resorcinol, formaldehyde solution and ammonia water are taken in the proportion of 1:1.5-1.8:0.4-0.45, and mixed. A mixed solution is obtained. The mixed solution is added dropwisely into the phenolic prepolymer solution. The mass proportion of the mixed solution and the Phenolic prepolymer solution is 1:40-45. Obtained solution is reacted for 24 hours at the stirring speed of 400-1000 revolutions per minute and undergoes hydro-thermal treatment under the temperature of 100 DEG C, and polymer nanospheres are obtained after an obtained product is dried for 2-12 hours under the temperature of 40-100 DEG C. The polymer nanospheres are protected by inert gas in a tube furnace and heated to 600-1000 DEG C for carbonization at the heating rate of 0.5-10 DEG C per minute. The porous carbon nanaspheres can be obtained when the temperature of the polymer nanospheres naturally lowers to the room temperature. The porous carbon nanosphere is controllable in diameter, good in dispersity, and improtant in application prospect in aspects such as catalysis, environment, energy and biology.