Serum-free medium and preparing method and application thereof

A technology of serum-free medium and culture supernatant, applied to bone/connective tissue cells, vertebrate cells, animal cells, etc. Complex serum components, unsatisfactory culture effects, etc., achieve the effect of maintaining proliferation ability and multi-directional differentiation potential, good proliferation ability and multi-directional differentiation potential, and eliminating the risk of spreading heterogeneous pathogens
CN105420189AInactive Publication Date: 2016-03-23郭镭 +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
郭镭
Publication Date
2016-03-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a novel serum-free medium. The serum-free medium comprises, by volume, 0.05-0.2 part of beta-mercaptoethanol, 0.5-2 parts of a non-essential amino acid aqueous solution, 4-6 parts of human mesenchymal stem cell cultural concentrated supernatant and 90-95 parts of a-MEM / DMEM-F12 and recombination human basic fibroblast growth factors with the final concentration of 5-15 ng / ml. A preparing method of the cultural concentrated supernatant includes the following steps that umbilical cord mesenchymal stem cell cultural supernatant is collected; cells, cell debris, impurities and the like are centrifugally removed; filtering is carried out through a micro-filtration membrane; ultrafiltration and concentration are carried out. When cultured through the medium, stem cells still keep the multi-directional differentiation potential and the high multiplication capacity under the long-time culturing condition.
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Description

technical field

[0001] The invention relates to the research field of stem cells, in particular to a novel, high-efficiency serum-free medium and its preparation method and application. Background technique

[0002] Mesenchymal stem cells are ubiquitous in various tissues and organs of the human body, have multi-directional differentiation potential, stimulate tissue regeneration, regulate immunity and other functions, and have broad application prospects in the field of cell therapy.

[0003] Bone marrow mesenchymal stem cells have been widely used clinically, and current research shows that umbilical cord-derived mesenchymal stem cells can not only become an ideal substitute for bone marrow mesenchymal stem cells, but also have greater application potential. Among them, human umbilical cord mesenchymal stem cells (humanUmbilicalCordmesenchymalstemcells, hUC-MSCs) derived from human umbilical cord express a variety of unique markers of embryonic stem cells, with high differ...

Claims

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