31 results about "Dmem f12" patented technology

The invention provides connexin core sequence-containing amphiphilic polypeptide AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH2, wherein the amphiphilic polypeptide and the amphiphilic polypeptide with the structure of AcN-RADARADARADARADA-CONH2 are triggered by Ca2+or a cell culture medium DMEM-F12 to automatically form into a compound, i.e. a nanometer-grade fiber material, and the nanometer-grade fiber material is taken as a bracket of the bone mesenchymal stem cells, so that the biological behavior of the cells can be obviously improved, the degenerated intervertebral disc can be repaired, and the original height can be recovered.

The invention belongs to the technical field of in-vitro establishment of tissue engineering cartilages, more particularly to an experimental method for establishing tissue engineering cartilage in vitro by taking a knee-joint cartilage unit of a rabbit as a seed cell. The experimental method comprises the following steps of: taking a keen joint of a rabbit, repeatedly cutting the joint cartilage into fragmentized tissues, washing, removing supernatant away and putting in a sterile conical flask for later use; adding dispase and II-type collagenase into the conical flask according to 12mL DMEM-F12 culture solution / g cartilage, stirring and digesting to form a digested suspension solution; filtering and centrifuging the digested suspension solution, adding a primary chondrocyte culture solution to prepare a sterile chondrocyte suspension solution, and carrying out sodium alginate gel three-dimensional culture on the obtained cartilage unit to construct the tissue engineering cartilage. The invention has the beneficial effects that: the cartilage unit has stable form and multiplication situation in the in-vitro long-term culturing process, the physiological function of substrate ingredients, such as secreted II-type collagen, and the like is improved, and the effect of repairing cartilage injury by adopting a tissue engineering method is expectedly accelerated.

A serum-free chondrocyte culture medium, in which exogenous additives are added to the basic culture medium. The basal culture fluid is DMEM culture fluid or F12 culture fluid or the mixed culture fluid of DMEM culture fluid and F12 culture fluid, and the exogenous additives are non-essential amino acids, dextran, vitamin C, glutamine, sodium pyruvate, insulin or Insulin-like growth factor, transferrin, growth hormone, triiodothyronine, hydrocortisone, dexamethasone, sodium selenite, beta-mercaptoethanol, lipid concentrate, progesterone, butylenediamine, base fibroblast growth factor, epidermal growth factor, transforming growth factor, bone morphogenic protein, platelet-derived growth factor. The culture medium does not add albumin, which can avoid the potential hidden dangers caused by the use of serum, improve the ability of chondrocytes to secrete extracellular matrix, and is close to the growth and proliferation state in the serum-containing culture medium. The improvement of matrix secretion ability is obviously better than that of serum-containing culture medium.